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  1. Article ; Online: Investigation of Factors Associated with Leg Trimmings at Processing: A 12-Year Review.

    Boyett, Taylor / Stayer, Phillip A / Correa, Maria / Crespo, Rocio

    Avian diseases

    2022  Volume 66, Issue 3, Page(s) 1–7

    Abstract: Gastrocnemius tendon injury or rupture is a common consequence from various etiologies and conditions in poultry production. The occurrence of tendon injury can cause significant morbidity and lameness in chickens, as well as quality downgrades and ... ...

    Abstract Gastrocnemius tendon injury or rupture is a common consequence from various etiologies and conditions in poultry production. The occurrence of tendon injury can cause significant morbidity and lameness in chickens, as well as quality downgrades and increased trimming on carcasses at processing. In this study, 12 yr of data from a poultry processing plant on leg trim only were compiled and analyzed. The association between grower, season, shift, and time were investigated in relation to the prevalence of ruptured tendons and carcass condemnations. A total of 8585 separate data entries from 195 growers were analyzed. Problem flocks were defined as those that had a carcass trimming rate of the percentage change equal to or above the 95th percentile of all data points (0.603%). We identified 430 instances of high trimmings in this study period, involving 90 growers. Of those, eight growers had 10 or more problem flocks in the study period. Overall, there were no differences in trimming rates due to shift; however, problem flocks had a higher trimming rate (percentage) during the night shift. A significantly higher rate of carcass trimmings was noted in the winter months. In problem flocks, a second and lower peak of a higher trimming was also observed in August. There was an upward trending carcass trimming that peaked in 2014, and it trended downward each year through 2020 overall. However, when the problem flocks were excluded, the trimming rate percentage change of trimming decreased slightly from 2008 to 2012 and remained steady through 2020. In conclusion, this study was able to demonstrate noninfectious causes that may be associated with increased leg trimmings and consequently made it possible to narrow down management practices to help decrease the instances of leg trimmings in the processing plant.
    MeSH term(s) Animals ; Chickens ; Poultry ; Prevalence ; Tendon Injuries/veterinary
    Language English
    Publishing date 2022-08-24
    Publishing country United States
    Document type Journal Article
    ZDB-ID 40871-2
    ISSN 1938-4351 ; 0005-2086
    ISSN (online) 1938-4351
    ISSN 0005-2086
    DOI 10.1637/aviandiseases-D-21-00111
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Investigation of Factors Associated with Leg Trimmings at Processing: A 12-Year Review

    Boyett, Taylor / Stayer, Phillip A. / Correa, Maria / Crespo, Rocio

    Avian Diseases. 2022 Aug. 24, v. 66, no. 3 p.345-351

    2022  

    Abstract: Gastrocnemius tendon injury or rupture is a common consequence from various etiologies and conditions in poultry production. The occurrence of tendon injury can cause significant morbidity and lameness in chickens, as well as quality downgrades and ... ...

    Abstract Gastrocnemius tendon injury or rupture is a common consequence from various etiologies and conditions in poultry production. The occurrence of tendon injury can cause significant morbidity and lameness in chickens, as well as quality downgrades and increased trimming on carcasses at processing. In this study, 12 yr of data from a poultry processing plant on leg trim only were compiled and analyzed. The association between grower, season, shift, and time were investigated in relation to the prevalence of ruptured tendons and carcass condemnations. A total of 8585 separate data entries from 195 growers were analyzed. Problem flocks were defined as those that had a carcass trimming rate of the percentage change equal to or above the 95th percentile of all data points (0.603%). We identified 430 instances of high trimmings in this study period, involving 90 growers. Of those, eight growers had 10 or more problem flocks in the study period. Overall, there were no differences in trimming rates due to shift; however, problem flocks had a higher trimming rate (percentage) during the night shift. A significantly higher rate of carcass trimmings was noted in the winter months. In problem flocks, a second and lower peak of a higher trimming was also observed in August. There was an upward trending carcass trimming that peaked in 2014, and it trended downward each year through 2020 overall. However, when the problem flocks were excluded, the trimming rate percentage change of trimming decreased slightly from 2008 to 2012 and remained steady through 2020. In conclusion, this study was able to demonstrate noninfectious causes that may be associated with increased leg trimmings and consequently made it possible to narrow down management practices to help decrease the instances of leg trimmings in the processing plant.
    Keywords birds ; lameness ; morbidity ; poultry ; poultry production ; condemnation ; leg trimming ; processing ; tendon rupture ; synovitis
    Language English
    Dates of publication 2022-0824
    Size p. 345-351.
    Publishing place American Association of Avian Pathologists Inc
    Document type Article ; Online
    ZDB-ID 40871-2
    ISSN 1938-4351 ; 0005-2086
    ISSN (online) 1938-4351
    ISSN 0005-2086
    DOI 10.1637/aviandiseases-D-21-00111
    Database NAL-Catalogue (AGRICOLA)

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  3. Article: Enumeration and speciation of coccidia affecting turkeys using flow cytometry method: Turkey coccidia diagnosis by Flow cytometry

    Boyett, Taylor / Crespo, Rocio / Vinueza, Valeria C / Gaghan, Carissa / Mohammed, Javid P / Kulkarni, Raveendra R

    Journal of applied poultry research. 2022,

    2022  

    Abstract: Enumeration of Eimeria oocysts is a common practice in monitoring coccidiosis in turkeys; however, the conventional method of manual microscopic examination of Eimeria oocysts is time-consuming. Previously, we used flow cytometry (FCM) to enumerate and ... ...

    Abstract Enumeration of Eimeria oocysts is a common practice in monitoring coccidiosis in turkeys; however, the conventional method of manual microscopic examination of Eimeria oocysts is time-consuming. Previously, we used flow cytometry (FCM) to enumerate and speciate coccidia affecting chickens and here, we extended those findings to turkey coccidia species, E. adenoides and E. meleagrimitis. Using FCM, a commercial vaccine containing these species was used to optimize the scatter-plot parameters, including Forward-(size) and Side-(shape/granularity) scatter Area, Height and Width patterns for Eimeria. The two Eimeria species populations in the vaccine were accurately phenotyped and the gated populations were then sorted using a Cell sorter instrument to obtain pure oocyst suspensions. The individual Eimeria species identity of sorted oocysts was confirmed by PCR using species-specific primers. A significant (p=0.0466) correlation (R=0.9893) in the total oocyst count between FCM and manual methods were observed. Furthermore, when FCM was employed to analyze farm fecal samples, the close similarities in the oocyst morphologies coupled with organic debris particulate interference prevented a precise separation of these two species resulting in a lack of oocyst count (OPG) correlation between the two methods. The OPG counts by FCM were much lower than the manual method; however, a partial OPG trend between the two methods was observed only at the early timepoint collections during a period of 35 days. Collectively, our findings showed that FCM can be used in the enumeration of turkey Eimeria oocysts with a potential scope for a more precise enumeration and speciation in field samples.
    Keywords coccidiosis ; farms ; flow cytometry ; microscopy ; oocysts ; research ; vaccines
    Language English
    Publishing place Elsevier Inc.
    Document type Article
    Note Pre-press version
    ZDB-ID 1193887-0
    ISSN 1537-0437 ; 1056-6171
    ISSN (online) 1537-0437
    ISSN 1056-6171
    DOI 10.1016/j.japr.2022.100270
    Database NAL-Catalogue (AGRICOLA)

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