LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 10 of total 91

Search options

  1. Article ; Online: The promise of optogenetic arrhythmia termination.

    Bruegmann, Tobias / Sacconi, Leonardo

    Journal of internal medicine

    2023  Volume 294, Issue 3, Page(s) 248–250

    MeSH term(s) Humans ; Optogenetics ; Arrhythmias, Cardiac
    Language English
    Publishing date 2023-06-12
    Publishing country England
    Document type Editorial ; Comment
    ZDB-ID 96274-0
    ISSN 1365-2796 ; 0954-6820
    ISSN (online) 1365-2796
    ISSN 0954-6820
    DOI 10.1111/joim.13680
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Ua VI Zs.44: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  2. Article: Editorial: Cardiac optogenetics: Using light to observe and excite the heart.

    Bruegmann, Tobias / Smith, Godfrey L / Lehnart, Stephan E

    Frontiers in physiology

    2022  Volume 13, Page(s) 1031062

    Language English
    Publishing date 2022-10-11
    Publishing country Switzerland
    Document type Editorial
    ZDB-ID 2564217-0
    ISSN 1664-042X
    ISSN 1664-042X
    DOI 10.3389/fphys.2022.1031062
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  3. Article ; Online: A Constitutively Active Cytokinin Receptor Variant Increases Cambial Activity and Stem Growth in Poplar.

    Riefler, Michael / Brügmann, Tobias / Fladung, Matthias / Schmülling, Thomas

    International journal of molecular sciences

    2022  Volume 23, Issue 15

    Abstract: The cambial meristem is responsible for bark and wood formation in woody plants. The activity of the cambial meristem is controlled by various factors; one of them is the plant hormone cytokinin. Here, we have explored different approaches to genetically ...

    Abstract The cambial meristem is responsible for bark and wood formation in woody plants. The activity of the cambial meristem is controlled by various factors; one of them is the plant hormone cytokinin. Here, we have explored different approaches to genetically engineering cambial activity in poplar plants by the ectopic expression of a cytokinin biosynthesis gene with enhanced activity (named
    MeSH term(s) Arabidopsis/metabolism ; Cambium/genetics ; Cambium/metabolism ; Cytokinins/metabolism ; Gene Expression Regulation, Plant ; Meristem/genetics ; Plants, Genetically Modified/metabolism ; Populus/metabolism
    Chemical Substances Cytokinins
    Language English
    Publishing date 2022-07-28
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms23158321
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  4. Article: Flexible DNA isolation procedure for different tree species as a convenient lab routine

    Bruegmann, Tobias / Fladung, Matthias / Schroeder, Hilke

    Silvae genetica. 2022 Jan. 01, v. 71, no. 1

    2022  

    Abstract: DNA isolation is a fundamental technique for all molecular biology laboratories. Depending on the plant species, DNA isolation can be challenging. In particular, adapted protocols rarely exist for tree species which are not used as standard model ... ...

    Abstract DNA isolation is a fundamental technique for all molecular biology laboratories. Depending on the plant species, DNA isolation can be challenging. In particular, adapted protocols rarely exist for tree species which are not used as standard model organisms. Here, we describe a flexible DNA isolation protocol that works for 59 tree species in a modular system. It is based on an ATMAB-containing extraction buffer to which proteinase K and/or boric acid are added, depending on the plant species. Subsequent purification steps include one or two precipitations with dichloromethane and, depending on the tree species, an optional sodium acetate precipitation. Using leaf material of a hybrid poplar clone from in vitro culture, it was determined that higher amounts of DNA could be isolated with this material than from field leaves. Starting from leaf material, DNA isolation for difficult cases was achieved with cambium or root tissue. This protocol was used to extract DNA for subsequent PCR amplification. Markers for cpDNA, mtDNA, and genomic DNA were used for standardized testing.
    Keywords Populus ; boric acid ; cambium ; chloroplast DNA ; hybrids ; leaves ; methylene chloride ; molecular biology ; peptidase K ; sodium acetate ; trees
    Language English
    Dates of publication 2022-0101
    Size p. 20-30.
    Publishing place Sciendo
    Document type Article
    ZDB-ID 2210420-3
    ISSN 2509-8934 ; 0037-5349
    ISSN (online) 2509-8934
    ISSN 0037-5349
    DOI 10.2478/sg-2022-0003
    Database NAL-Catalogue (AGRICOLA)

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  5. Book ; Online ; Thesis: Direct optogenetic stimulation of smooth muscle cells to control gastric contractility

    Vogt, Markus [Verfasser] / Brügmann, Tobias [Akademischer Betreuer] / Brügmann, Tobias [Gutachter] / Heßmann, Elisabeth [Gutachter]

    2023  

    Author's details Markus Vogt ; Gutachter: Tobias Brügmann, Elisabeth Heßmann ; Betreuer: Tobias Brügmann
    Keywords Medizin, Gesundheit ; Medicine, Health
    Subject code sg610
    Language German
    Publisher Niedersächsische Staats- und Universitätsbibliothek Göttingen
    Publishing place Göttingen
    Document type Book ; Online ; Thesis
    Database Digital theses on the web

    Full text online

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  6. Article ; Online: Changes in wax composition but not amount enhance cuticular transpiration

    Grünhofer, Paul / Herzig, Lena / Zhang, Qihui / Vitt, Simon / Stöcker, Tyll / Malkowsky, Yaron / Brügmann, Tobias / Fladung, Matthias / Schreiber, Lukas

    Plant, Cell & Environment. 2024 Jan., v. 47, no. 1 p.91-105

    2024  

    Abstract: This study focuses on the role of the qualitative leaf wax composition in modulating the cuticular water loss using a Populus × canescens cer6 mutant line, which accumulates C34–C46 wax ester dimers and is reduced in wax monomers >C24. The two literature‐ ...

    Abstract This study focuses on the role of the qualitative leaf wax composition in modulating the cuticular water loss using a Populus × canescens cer6 mutant line, which accumulates C34–C46 wax ester dimers and is reduced in wax monomers >C24. The two literature‐based hypotheses to be tested were the importance of the amount of wax esters and the weighted mean carbon chain length in restricting cuticular water loss. The main results were acquired by chemical analysis of cuticular wax and gravimetric cuticular transpiration measurements. Besides additional physiological measurements, the leaf surface properties were characterised by scanning electron microscopy and spectrophotometric light reflectance quantification. Mutation of the CER6 gene resulted in striking changes in qualitative wax composition but not quantitative wax amount. Based on the strong accumulation of dimeric wax esters, the relative proportion of esters increased to >90%, and the weighted mean carbon chain length increased by >6 carbon atoms. These qualitative alterations were found to increase the cuticular transpiration of leaves by twofold. Our results do not support the hypotheses that enhanced amounts of wax esters or increased weighted mean carbon chain lengths of waxes lead to reduced cuticular transpiration.
    Keywords Populus ; carbon ; chemical analysis ; electron microscopy ; environment ; epicuticular wax ; genes ; leaves ; mutants ; mutation ; reflectance ; transpiration
    Language English
    Dates of publication 2024-01
    Size p. 91-105.
    Publishing place John Wiley & Sons, Ltd
    Document type Article ; Online
    Note JOURNAL ARTICLE
    ZDB-ID 391893-2
    ISSN 1365-3040 ; 0140-7791
    ISSN (online) 1365-3040
    ISSN 0140-7791
    DOI 10.1111/pce.14719
    Database NAL-Catalogue (AGRICOLA)

    More links

    Kategorien

    In stock of ZB MED Bonn / Germany

    Z 80/729: Show issues

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  7. Book ; Online ; Thesis: Optogenetics in striated muscle: defibrillation of the heart and direct stimulation of skeletal muscles with light

    Brügmann, Tobias [Verfasser]

    2019  

    Author's details Tobias Brügmann
    Keywords Naturwissenschaften ; Science
    Subject code sg500
    Language English
    Publisher Universitäts- und Landesbibliothek Bonn
    Publishing place Bonn
    Document type Book ; Online ; Thesis
    Database Digital theses on the web

    Full text online

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  8. Article ; Online: Steigerung der Trockenstresstoleranz in Bäumen durch genetische Modifikationen

    Fendel, Alexander / Fladung, Matthias / Brügmann, Tobias

    2023  

    Keywords Text ; ddc:630
    Language German
    Publishing country de
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  9. Article: Optogenetic Stimulation of G

    Cokić, Milan / Bruegmann, Tobias / Sasse, Philipp / Malan, Daniela

    Frontiers in physiology

    2021  Volume 12, Page(s) 768495

    Abstract: G-protein signaling pathways are central in the regulation of cardiac function in physiological and pathophysiological conditions. Their functional analysis through optogenetic techniques with selective expression of opsin proteins and activation by ... ...

    Abstract G-protein signaling pathways are central in the regulation of cardiac function in physiological and pathophysiological conditions. Their functional analysis through optogenetic techniques with selective expression of opsin proteins and activation by specific wavelengths allows high spatial and temporal precision. Here, we present the application of long wavelength-sensitive cone opsin (LWO) in cardiomyocytes for activation of the G
    Language English
    Publishing date 2021-12-20
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2564217-0
    ISSN 1664-042X
    ISSN 1664-042X
    DOI 10.3389/fphys.2021.768495
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  10. Article ; Online: No light without the dark: Perspectives and hindrances for translation of cardiac optogenetics.

    Richter, Claudia / Bruegmann, Tobias

    Progress in biophysics and molecular biology

    2019  Volume 154, Page(s) 39–50

    Abstract: Over the last decade, optogenetic stimulation of the heart and its translational potential for rhythm control attracted more and more interest. Optogenetics allows to stimulate cardiomyocytes expressing the light-gated cation channel Channelrhodopsin 2 ( ... ...

    Abstract Over the last decade, optogenetic stimulation of the heart and its translational potential for rhythm control attracted more and more interest. Optogenetics allows to stimulate cardiomyocytes expressing the light-gated cation channel Channelrhodopsin 2 (ChR2) with light and thus high spatio-temporal precision. Therefore this new approach can overcome the technical limitations of electrical stimulation. In regard of translational approaches, the prospect of pain-free stimulation, if ChR2 expression is restricted to cardiomyocytes, is especially intriguing and could be highly beneficial for cardioversion and defibrillation. However, there is no light without shadow and cardiac optogenetics has to surmount critical hurdles, namely "how" to inscribe light-sensitivity by expressing ChR2 in a native heart and how to avoid side effects such as possible immune responses against the gene transfer. Furthermore, implantable light devices have to be developed which ensure sufficient illumination in a highly contractile environment. Therefore this article reviews recent advantages in the field of cardiac optogenetics with a special focus on the hindrances for the potential translation of this new approach into clinics and provides an outlook how these have to be carefully investigated and could be solved step by step.
    MeSH term(s) Animals ; Darkness ; Heart/physiology ; Heart/radiation effects ; Humans ; Optogenetics/instrumentation ; Optogenetics/methods
    Language English
    Publishing date 2019-09-09
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 209302-9
    ISSN 1873-1732 ; 0079-6107
    ISSN (online) 1873-1732
    ISSN 0079-6107
    DOI 10.1016/j.pbiomolbio.2019.08.013
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Uc I Zs.281: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

To top