LIVIVO - Search results -

Search results

Result 1 - 9 of total 9

Search options

Article: Intra- and Extra-Hospital Dissemination of IMP-22-Producing

Gonçalves, Daniela / Cecílio, Pedro / Faustino, Alberta / Iglesias, Carmen / Branca, Fernando / Estrada, Alexandra / Ferreira, Helena

Frontiers in microbiology

2021 Volume 12, Page(s) 777054

Abstract: The emergence of infections (and colonization) ... ...

Abstract	The emergence of infections (and colonization) with
Language	English
Publishing date	2021-12-14
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2587354-4
ISSN	1664-302X
ISSN	1664-302X
DOI	10.3389/fmicb.2021.777054
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Article ; Online: Sampling Methods and Risk Stratification Regarding Environmental Contamination by SARS-CoV-2.

Mendes, Marta / Andrade Oliveira, Ana / Pires, Olga / Branca, Fernando / Beirão, Maria / Santa-Cruz, André / Carvalho, Alexandre / Alves, Joana

Acta medica portuguesa

2021 Volume 34, Issue 12, Page(s) 851–856

Abstract: Introduction: Transmission of COVID-19 through close contact and droplets is well established, but the influence of aerosol and surface contamination remains to be determined. Literature is scarce and inconsistent about the viable virus particles free- ... ...

Abstract	Introduction: Transmission of COVID-19 through close contact and droplets is well established, but the influence of aerosol and surface contamination remains to be determined. Literature is scarce and inconsistent about the viable virus particles free-distance from infected patients, as well as about different swabbing methods for surface contamination evaluation. The aim of this study was to evaluate the most sensitive method for the assessment of surface contamination, classify the likelihood of environmental contamination in risk zones and compare the environmental contamination between oxygenation and ventilatory support. Material and methods: Swabs from potentially contaminated surfaces in a COVID-19 ward, with patients treated with different types of oxygen and ventilatory support, were collected. Three types of swabs were compared in order to evaluate the most sensitive collection method. For risk zone categorization, areas were divided according to the distance from the patient. Results: Of the 63 swabs collected, 17 (27%) tested positive for the presence of SARS-CoV-2. The highest positivity rate was observed with the sterile premoistened swab with saline (n = 8; 38%), but without statistically significant differences. The highest number of positive samples were collected from the high-risk zones, specifically those located one meter from the patient (n = 13; 48%), with statistically significant differences. Only the rooms of patients supported with non-invasive ventilation or high-flow nasal cannula had evidence of bedroom contamination, with 45% and 27% of swab positivity, with statistically significant differences. Discussion: Our findings favour the premoistened swab without transport medium for surface contamination assessment, even though without statistical differences. A statistically significant trend supporting the division in risk zones, according to the distance from the patient, was also identified. The higher positivity rate from the non-invasive ventilation and high-flow nasal cannula bedrooms suggests a significant association between ventilatory strategies and surface contamination, probably due to higher particle dispersion. Conclusion: Our findings support the use of the sterile premoistened swab without preservation medium, the classification of risk areas considering the distance from the patient, and the variability of RNA dispersion between oxygenation and ventilatory support.
MeSH term(s)	COVID-19 ; Hospitals ; Humans ; Respiration, Artificial ; Risk Assessment ; SARS-CoV-2
Language	English
Publishing date	2021-05-20
Publishing country	Portugal
Document type	Journal Article
ZDB-ID	603078-6
ISSN	1646-0758 ; 0870-399X
ISSN (online)	1646-0758
ISSN	0870-399X
DOI	10.20344/amp.16215
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.B 2343: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: OmniSARS2: A Highly Sensitive and Specific RT-qPCR-Based COVID-19 Diagnostic Method Designed to Withstand SARS-CoV-2 Lineage Evolution.

Carvalho-Correia, Eduarda / Calçada, Carla / Branca, Fernando / Estévez-Gómez, Nuria / De Chiara, Loretta / Varela, Nair / Gallego-García, Pilar / Posada, David / Sousa, Hugo / Sousa, João / Veiga, Maria Isabel / Osório, Nuno S

Biomedicines

2021 Volume 9, Issue 10

Abstract: Extensive transmission of SARS-CoV-2 during the COVID-19 pandemic allowed the generation of thousands of mutations within its genome. While several of these become rare, others largely increase in prevalence, potentially jeopardizing the sensitivity of ... ...

Abstract	Extensive transmission of SARS-CoV-2 during the COVID-19 pandemic allowed the generation of thousands of mutations within its genome. While several of these become rare, others largely increase in prevalence, potentially jeopardizing the sensitivity of PCR-based diagnostics. Taking advantage of SARS-CoV-2 genomic knowledge, we designed a one-step probe-based multiplex RT-qPCR (OmniSARS2) to simultaneously detect short fragments of the SARS-CoV-2 genome in ORF1ab, E gene and S gene. Comparative genomics of the most common SARS-CoV-2 lineages, other human betacoronavirus and alphacoronavirus, was the basis for this design, targeting both highly conserved regions across SARS-CoV-2 lineages and variable or absent in other
Language	English
Publishing date	2021-09-26
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2720867-9
ISSN	2227-9059
ISSN	2227-9059
DOI	10.3390/biomedicines9101314
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: High level biosynthesis of a silk-elastin-like protein in E. coli.

Collins, Tony / Barroca, Mário / Branca, Fernando / Padrão, Jorge / Machado, Raul / Casal, Margarida

Biomacromolecules

2014 Volume 15, Issue 7, Page(s) 2701–2708

Abstract: Silk-elastin-like proteins (SELPs) have enormous potential for use as customizable biomaterials in numerous biomedical and materials applications, yet success in harnessing this potential has been limited by the lack of a commercially viable industrially ...

Abstract	Silk-elastin-like proteins (SELPs) have enormous potential for use as customizable biomaterials in numerous biomedical and materials applications, yet success in harnessing this potential has been limited by the lack of a commercially viable industrially relevant production process. We have developed a scalable fed-batch production approach which enables a SELP volumetric productivity of 4.3 g L(-1) with E. coli BL21(DE3). This is the highest SELP productivity reported to date and is 50-fold higher than that reported by other groups. As compared to typical fed-batch processes, high preinduction growth rates and low inducer and oxygen concentrations are allowed whereas reduced postinduction feeding rates are preferred. Limiting factors were identified and productivity was found to be strongly influenced by a trade-off between the rate of production and plasmid stability. The process developed is robust, reproducible, and applicable to scale up to the industrial level and moves these biopolymers a step closer to the marketplace.
MeSH term(s)	Batch Cell Culture Techniques ; Elastin/biosynthesis ; Escherichia coli/growth & development ; Escherichia coli/metabolism ; Recombinant Fusion Proteins/biosynthesis ; Silk/biosynthesis
Chemical Substances	Recombinant Fusion Proteins ; Silk ; Elastin (9007-58-3)
Language	English
Publishing date	2014-07-14
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1526-4602
ISSN (online)	1526-4602
DOI	10.1021/bm5005564
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article: Acute Alithiasic Cholecystitis and Human Herpes Virus Type-6 Infection: First Case.

Gomes, Maria Miguel / Antunes, Henedina / Lobo, Ana Luísa / Branca, Fernando / Correia-Pinto, Jorge / Moreira-Pinto, João

Case reports in pediatrics

2016 Volume 2016, Page(s) 9130673

Abstract: A three-year-old male child presented with erythematous maculopapular nonpruritic generalized rash, poor feeding, vomiting, and cramping generalized abdominal pain. He was previously healthy and there was no family history of immunologic or other ... ...

Abstract	A three-year-old male child presented with erythematous maculopapular nonpruritic generalized rash, poor feeding, vomiting, and cramping generalized abdominal pain. He was previously healthy and there was no family history of immunologic or other diseases. On examination he was afebrile, hemodynamically stable, with painful palpation of the right upper quadrant and positive Murphy's sign. Laboratory tests revealed elevated inflammatory markers, elevated aminotransferase activity, and features of cholestasis. Abdominal ultrasound showed gallbladder wall thickening of 8 mm with a positive sonographic Murphy's sign, without gallstones or pericholecystic fluid. Acute Alithiasic Cholecystitis (AAC) was diagnosed. Tests for underlying infectious causes were negative except positive blood specimen for Human Herpes Virus Type-6 (HHV-6) by polymerase chain reaction. With supportive therapy the child became progressively less symptomatic with gradual improvement. The child was discharged on the sixth day, asymptomatic and with improved analytic values. Two months later he had IgM negative and IgG positive antibodies (1/160) for HHV-6, which confirmed the diagnosis of previous infection. In a six-month follow-up period he remains asymptomatic. To the best of our knowledge, this represents the first case of AAC associated with HHV-6 infection.
Language	English
Publishing date	2016-04-20
Publishing country	United States
Document type	Journal Article
ZDB-ID	2659094-3
ISSN	2090-6811 ; 2090-6803
ISSN (online)	2090-6811
ISSN	2090-6803
DOI	10.1155/2016/9130673
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article: High Level Biosynthesis of a Silk-Elastin-like Protein in E. coli

Collins, Tony / Barroca MaÌrio / Branca Fernando / PadraÌo Jorge / Machado Raul / Casal Margarida

Biomacromolecules. 2014 July 14, v. 15, no. 7

2014

Abstract	Silk-elastin-like proteins (SELPs) have enormous potential for use as customizable biomaterials in numerous biomedical and materials applications, yet success in harnessing this potential has been limited by the lack of a commercially viable industrially relevant production process. We have developed a scalable fed-batch production approach which enables a SELP volumetric productivity of 4.3 g LâÂ¹ with E. coli BL21(DE3). This is the highest SELP productivity reported to date and is 50-fold higher than that reported by other groups. As compared to typical fed-batch processes, high preinduction growth rates and low inducer and oxygen concentrations are allowed whereas reduced postinduction feeding rates are preferred. Limiting factors were identified and productivity was found to be strongly influenced by a trade-off between the rate of production and plasmid stability. The process developed is robust, reproducible, and applicable to scale up to the industrial level and moves these biopolymers a step closer to the marketplace.
Keywords	Escherichia coli ; biocompatible materials ; biopolymers ; biosynthesis ; oxygen ; plasmids ; proteins
Language	English
Dates of publication	2014-0714
Size	p. 2701-2708.
Publishing place	American Chemical Society
Document type	Article
ISSN	1526-4602
DOI	10.1021%2Fbm5005564
Database	NAL-Catalogue (AGRICOLA)

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Batch production of a silk-elastin-like protein in E. coli BL21(DE3): key parameters for optimisation.

Collins, Tony / Azevedo-Silva, João / da Costa, André / Branca, Fernando / Machado, Raul / Casal, Margarida

Microbial cell factories

2013 Volume 12, Page(s) 21

Abstract: Background: Silk-elastin-like proteins (SELPs) combining the physicochemical and biological properties of silk and elastin have a high potential for use in the pharmaceutical, regenerative medicine and materials fields. Their development for use is ... ...

Abstract	Background: Silk-elastin-like proteins (SELPs) combining the physicochemical and biological properties of silk and elastin have a high potential for use in the pharmaceutical, regenerative medicine and materials fields. Their development for use is however restrained by their production levels. Here we describe the batch production optimisation for a novel recently described SELP in the pET-E. coli BL21(DE3) expression system. Both a comprehensive empirical approach examining all process variables (media, induction time and period, temperature, pH, aeration and agitation) and a detailed characterisation of the bioprocess were carried out in an attempt to maximise production with this system. Results: This study shows that maximum SELP volumetric production is achieved at 37°C using terrific broth at pH 6-7.5, a shake flask volume to medium volume ratio of 10:1 and an agitation speed of 200 rpm. Maximum induction is attained at the beginning of the stationary phase with 0.5 mM IPTG and an induction period of at least 4 hours. We show that the selection agents ampicillin and carbenicillin are rapidly degraded early in the cultivation and that plasmid stability decreases dramatically on induction. Furthermore, acetate accumulates during the bioprocess to levels which are shown to be inhibitory to the host cells. Using our optimised conditions, 500 mg/L of purified SELP was obtained. Conclusions: We have identified the optimal conditions for the shake flask production of a novel SELP with the final production levels obtained being the highest reported to date. While this study is focused on SELPs, we believe that it could also be of general interest to any study where the pET (ampicillin selective marker)-E. coli BL21(DE3) expression system is used. In particular, we show that induction time is critical in this system with, in contrast to that which is generally believed, optimal production being obtained by induction at the beginning of the stationary phase. Furthermore, we believe that we are at or near the maximum productivity for the system used, with rapid degradation of the selective agent by plasmid encoded β-lactamase, plasmid instability on induction and high acetate production levels being the principal limiting factors for further improved production.
MeSH term(s)	Biomass ; Elastin/metabolism ; Escherichia coli/growth & development ; Escherichia coli/metabolism ; Genetic Vectors/genetics ; Genetic Vectors/metabolism ; Hydrogen-Ion Concentration ; Plasmids/genetics ; Plasmids/metabolism ; Proteins/genetics ; Proteins/metabolism ; Recombinant Proteins/biosynthesis ; Recombinant Proteins/genetics ; Silk/metabolism ; Temperature
Chemical Substances	Proteins ; Recombinant Proteins ; Silk ; Elastin (9007-58-3)
Language	English
Publishing date	2013-02-27
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1475-2859
ISSN (online)	1475-2859
DOI	10.1186/1475-2859-12-21
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Analysis of a local HIV-1 epidemic in portugal highlights established transmission of non-B and non-G subtypes.

Carvalho, Alexandre / Costa, Patrício / Triunfante, Vera / Branca, Fernando / Rodrigues, Fernando / Santos, Catarina L / Correia-Neves, Margarida / Saraiva, Margarida / Lecour, Henrique / Castro, António G / Pedrosa, Jorge / Osório, Nuno S

Journal of clinical microbiology

2015 Volume 53, Issue 5, Page(s) 1506–1514

Abstract: The existing data support Portugal as the western European country with the highest HIV-1 subtype diversity. However, detailed phylogenetic studies of Portuguese HIV-1 epidemics are still scarce. Thus, our main goal was to analyze the phylodynamics of a ... ...

Abstract	The existing data support Portugal as the western European country with the highest HIV-1 subtype diversity. However, detailed phylogenetic studies of Portuguese HIV-1 epidemics are still scarce. Thus, our main goal was to analyze the phylodynamics of a local HIV-1 infection in the Portuguese region of Minho. Molecular epidemiological analysis was applied to data from 289 HIV-1-infected individuals followed at the reference hospital of the province of Minho, Portugal, at which isolated viruses had been sequenced between 2000 and 2012. Viruses of the G (29.1%) and B (27.0%) subtypes were the most frequent, followed by recombinant forms (17.6%) and the C (14.5%), F1 (7.3%), and A1 (4.2%) subtypes. Multinomial logistic regression revealed that the odds of being infected with the A1 and F1 subtypes increased over the years compared with those with B, G, or C subtypes or recombinant viruses. As expected, polyphyletic patterns suggesting multiple and old introductions of the B and G subtypes were found. However, transmission clusters of non-B and non-G viruses among native individuals were also found, with the dates of the most recent common ancestor estimated to be in the early 2000s. Our study supports that the HIV-1 subtype diversity in the Portuguese region of Minho is high and has been increasing in a manner that is apparently driven by factors other than immigration and international travel. Infections with A1 and F1 viruses in the region of Minho are becoming established and are mainly found in sexually transmitted clusters, reinforcing the need for more efficacious control measures targeting this infection route.
MeSH term(s)	Adolescent ; Adult ; Aged ; Aged, 80 and over ; Cohort Studies ; Epidemics ; Female ; Genetic Variation ; Genotype ; HIV Infections/epidemiology ; HIV Infections/transmission ; HIV Infections/virology ; HIV-1/classification ; HIV-1/genetics ; HIV-1/isolation & purification ; Humans ; Male ; Middle Aged ; Molecular Epidemiology ; Phylogeny ; Portugal/epidemiology ; Prevalence ; Sequence Analysis, DNA ; Young Adult
Language	English
Publishing date	2015-05
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	390499-4
ISSN	1098-660X ; 0095-1137
ISSN (online)	1098-660X
ISSN	0095-1137
DOI	10.1128/JCM.03611-14
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Full text

In stock of ZB MED Cologne/Königswinter

Zs.A 1188: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

Article ; Online: G2P[4] the most prevalent rotavirus genotype in 2007 winter season in an European non-vaccinated population.

Antunes, Henedina / Afonso, Ariana / Iturriza, Miren / Martinho, Isabel / Ribeiro, Cristiana / Rocha, Sandra / Magalhães, Catarina / Carvalho, Liliana / Branca, Fernando / Gray, Jim

Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology

2009 Volume 45, Issue 1, Page(s) 76–78

Abstract: Background: Recently, a high prevalence of G2P[4] rotavirus (RV) infection was reported from Brazil, and linked with the universal RV vaccination programme that used the G1P[8] live oral RV vaccine.: Objective: To determine the genotypes of RV co- ... ...

Abstract	Background: Recently, a high prevalence of G2P[4] rotavirus (RV) infection was reported from Brazil, and linked with the universal RV vaccination programme that used the G1P[8] live oral RV vaccine. Objective: To determine the genotypes of RV co-circulating in a non-vaccinated population, in northern Portugal in the winter season of 2007. Study design: Prospective multicenter study of the genotypes circulating in the northwest region of Portugal during January to March 2007. Children with acute gastroenteritis, who attended the Pediatric Emergency Services of five Hospitals, were included in the study. The parents of the children completed a clinical and epidemiological data questionnaire and stool samples were collected. Stool samples positive in a RV enzyme immunoassay (EIA) were genotyped by reverse transcriptase-polymerase chain reaction. Results: Stool samples were collected from 424 children. Two hundred and thirty-four (55.2%) stool samples were RV-positive. G2P[4] was the predominant RV type (68.6%), followed by G9P[8] (14.0%). Conclusions: Because our population was naïve for RV vaccine, the G2P[4] predominance cannot be explained by vaccination. Rather, this high prevalence of G2P[4] may be within the normal fluctuation of RV genotypes. RV strain surveillance programmes are important for informing RV vaccination programmes.
MeSH term(s)	Acute Disease/epidemiology ; Child ; Child, Preschool ; Feces/virology ; Female ; Gastroenteritis/epidemiology ; Gastroenteritis/virology ; Genotype ; Humans ; Immunoenzyme Techniques ; Infant ; Male ; Portugal/epidemiology ; Prospective Studies ; Reverse Transcriptase Polymerase Chain Reaction ; Rotavirus/classification ; Rotavirus/genetics ; Rotavirus/isolation & purification ; Rotavirus Infections/epidemiology ; Rotavirus Infections/virology ; Rotavirus Vaccines ; Seroepidemiologic Studies ; Surveys and Questionnaires
Chemical Substances	Rotavirus Vaccines
Language	English
Publishing date	2009-05
Publishing country	Netherlands
Document type	Journal Article ; Multicenter Study ; Research Support, Non-U.S. Gov't
ZDB-ID	1446080-4
ISSN	1873-5967 ; 1386-6532
ISSN (online)	1873-5967
ISSN	1386-6532
DOI	10.1016/j.jcv.2009.03.010
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 3790: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

To top

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito