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  1. Article ; Online: The Regina Elena National Cancer Institute process of accreditation according to the standards of the Organisation of European Cancer Institutes.

    Canitano, Stefano / Di Turi, Annunziata / Caolo, Giuseppina / Pignatelli, Adriana C / Papa, Elena / Branca, Marta / Cerimele, Marina / De Maria, Ruggero

    Tumori

    2015  Volume 101 Suppl 1, Page(s) S51–4

    Abstract: The accreditation process is, on the one hand, a tool used to homogenize procedures, rendering comparable and standardized processes of care, and on the other, a methodology employed to develop a culture of quality improvement. Although not yet proven by ...

    Abstract The accreditation process is, on the one hand, a tool used to homogenize procedures, rendering comparable and standardized processes of care, and on the other, a methodology employed to develop a culture of quality improvement. Although not yet proven by evidence-based studies that health outcomes improve as a result of an accreditation to excellence, it is undeniable that better control of healthcare processes results in better quality and safety of diagnostic and therapeutic pathways. The Regina Elena National Cancer Institute underwent the accreditation process in accordance with the standards criteria set by the Organisation of European Cancer Institutes (OECI), and it has recently completed the process, acquiring its designation as a Comprehensive Cancer Center (CCC). This was an invaluable opportunity for the Regina Elena Institute to create a more cohesive environment, to widely establish a culture of quality, to implement an institutional information system, and to accelerate the process of patient involvement in strategic decisions. The steps of the process allowed us to evaluate the performance and the organization of the institute and put amendments in place designed to be adopted through 26 improvement actions. These actions regarded several aspects of the institute, including quality culture, information communication technology system, care, clinical trials unit, disease management team, nursing, and patient empowerment and involvement. Each area has a timeline. We chose to present the following 3 improvement actions: clinical trial center, computerized ambulatory medical record, and centrality of patient and humanization of clinical pathway.
    MeSH term(s) Accreditation/methods ; Ambulatory Care/standards ; Cancer Care Facilities/standards ; Clinical Trials as Topic/standards ; Critical Pathways/organization & administration ; Critical Pathways/standards ; Europe ; Humans ; International Cooperation ; Italy ; Medical Oncology/organization & administration ; Medical Oncology/standards ; Quality Assurance, Health Care ; Quality Improvement ; Quality of Health Care
    Language English
    Publishing date 2015
    Publishing country Italy
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 280962-x
    ISSN 2038-2529 ; 0300-8916
    ISSN (online) 2038-2529
    ISSN 0300-8916
    DOI 10.5301/tj.5000461
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: 2019-novel Coronavirus severe adult respiratory distress syndrome in two cases in Italy

    Albarello, Fabrizio / Pianura, Elisa / Di Stefano, Federica / Cristofaro, Massimo / Petrone, Ada / Marchioni, Luisa / Palazzolo, Claudia / Schininà, Vincenzo / Nicastri, Emanuele / Petrosillo, Nicola / Campioni, Paolo / Zumla, Alimuddin / Eskild, Petersen / Ippolito, Giuseppe / Abbonizio, Maria Alessandra / Agrati, Chiara / Amadei, Gioia / Amendola, Alessandra / Antonini, Mario /
    Barbaro, Raffaella / Bartolini, Barbara / Benigni, Martina / Bevilacqua, Nazario / Bordi, Licia / Bordoni, Veronica / Branca, Marta / Capobianchi, Maria Rosaria / Caporale, Cinzia / Caravella, Ilaria / Carletti, Fabrizio / Castilletti, Concetta / Chiappini, Roberta / Ciaralli, Carmine / Colavita, Francesca / Corpolongo, Angela / Curiale, Salvatore / D'Abramo, Alessandra / Dantimi, Cristina / Angelis, Alessia De / Angelis, Giada De / Lorenzo, Rachele Di / Stefano, Federica Di / Ferraro, Federica / Fiorentini, Lorena / Frustaci, Andrea / Gallì, Paola / Garotto, Gabriele / Giancola, Maria Letizia / Giansante, Filippo / Giombini, Emanuela / Greci, Maria Cristina / Lalle, Eleonora / Lanini, Simone / Lapa, Daniele / Lepore, Luciana / Lucia, Andrea / Lufrani, Franco / Macchione, Manuela / Marani, Alessandra / Mariano, Andrea / Marini, Maria Cristina / Maritti, Micaela / Matusali, Giulia / Meschi, Silvia / Montaldo, Francesco Messina Chiara / Murachelli, Silvia / Noto, Roberto / Pallini, Emanuele / Passeri, Virgilio / Pelliccioni, Federico / Petrecchia, Antonella / Pisciotta, Maria / Pittalis, Silvia / Proietti, Costanza / Puro, Vincenzo / Rinonapoli, Gabriele / Rueca, Martina / Sacchi, Alessandra / Sanasi, Francesco / Santagata, Carmen / Scarcia, Silvana / Scognamiglio, Paola / Scorzolini, Laura / Stazi, Giulia / Vaia, Francesco / Vairo, Francesco / Valli, Maria Beatrice

    reponame:Expeditio Repositorio Institucional UJTL ; instname:Universidad de Bogotá Jorge Tadeo Lozano

    An uncommon radiological presentation

    2020  

    Abstract: Introduction Several recent case reports have described common early chest imaging findings of lung pathology caused by 2019 novel Coronavirus (SARS-COV2) which appear to be similar to those seen previously in SARS-CoV and MERS-CoV infected patients. ... ...

    Abstract Introduction Several recent case reports have described common early chest imaging findings of lung pathology caused by 2019 novel Coronavirus (SARS-COV2) which appear to be similar to those seen previously in SARS-CoV and MERS-CoV infected patients. Objective We present some remarkable imaging findings of the first two patients identified in Italy with COVID-19 infection travelling from Wuhan, China. The follow-up with chest X-Rays and CT scans was also included, showing a progressive adult respiratory distress syndrome (ARDS). Results Moderate to severe progression of the lung infiltrates, with increasing percentage of high-density infiltrates sustained by a bilateral and multi-segmental extension of lung opacities, were seen. During the follow-up, apart from pleural effusions, a tubular and enlarged appearance of pulmonary vessels with a sudden caliber reduction was seen, mainly found in the dichotomic tracts, where the center of a new insurgent pulmonary lesion was seen. It could be an early alert radiological sign to predict initial lung deterioration. Another uncommon element was the presence of mediastinal lymphadenopathy with short-axis oval nodes. Conclusions Although only two patients have been studied, these findings are consistent with the radiological pattern described in literature. Finally, the pulmonary vessels enlargement in areas where new lung infiltrates develop in the follow-up CT scan, could describe an early predictor radiological sign of lung impairment.
    Keywords Vasos pulmonares agrandados ; Síndrome respiratorio agudo grave ; COVID-19 ; SARS-CoV-2 ; Coronavirus ; SARS-COV2 ; CT-scan ; Ground glass opacities ; Crazy-paving ; covid19
    Publisher International Journal of Infectious Diseases
    Publishing country co
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: Pharmacokinetics of remdesivir and GS-441524 in two critically ill patients who recovered from COVID-19

    Tempestilli, Massimo / Caputi, Priscilla / Avataneo, Valeria / Notari, Stefania / Forini, Olindo / Scorzolini, Laura / Marchioni, Luisa / Ascoli Bartoli, Tommaso / Castilletti, Concetta / Lalle, Eleonora / Capobianchi, Maria R / Nicastri, Emanuele / D’Avolio, Antonio / Ippolito, Giuseppe / Agrati, Chiara / Abbonizio, Maria Alessandra / Albarello, Fabrizio / Amadei, Gioia / Amendola, Alessandra /
    Antinori, Andrea / Antonini, Mario / Barbaro, Raffaella / Bartolini, Barbara / Benigni, Martina / Bevilacqua, Nazario / Bordi, Licia / Bordoni, Veronica / Branca, Marta / Campioni, Paolo / Capobianchi, Maria Rosaria / Caporale, Cinzia / Caravella, Ilaria / Carletti, Fabrizio / Casetti, Rita / Chiappini, Roberta / Ciaralli, Carmine / Cimini, Eleonora / Colavita, Francesca / Corpolongo, Angela / Cristofaro, Massimo / Curiale, Salvatore / D’Abramo, Alessandra / Dantimi, Cristina / De Angelis, Alessia / De Angelis, Giada / Di Lorenzo, Rachele / Di Stefano, Federica / D’Offizi, Gianpiero / Ferraro, Federica / Fiorentini, Lorena / Frustaci, Andrea / Gallì, Paola / Garotto, Gabriele / Giancola, Maria Letizia / Giansante, Filippo / Giombini, Emanuela / Grassi, Germana / Greci, Maria Cristina / Lanini, Simone / Lapa, Daniele / Lepore, Luciana / Lucia, Andrea / Lufrani, Franco / Macchione, Manuela / Marani, Alessandra / Mariano, Andrea / Marini, Maria Cristina / Maritti, Micaela / Matusali, Giulia / Meschi, Silvia / Messina, Francesco / Montaldo, Chiara / Murachelli, Silvia / Noto, Roberto / Palazzolo, Claudia / Pallini, Emanuele / Palmieri, Fabrizio / Passeri, Virgilio / Pelliccioni, Federico / Petrecchia, Antonella / Petrone, Ada / Petrosillo, Nicola / Pianura, Elisa / Pisciotta, Maria / Pittalis, Silvia / Proietti, Costanza / Puro, Vincenzo / Rinonapoli, Gabriele / Rueca, Martina / Sacchi, Alessandra / Sanasi, Francesco / Santagata, Carmen / Scarcia, Silvana / Schininà, Vincenzo / Scognamiglio, Paola / Stazi, Giulia / Vaia, Francesco / Vairo, Francesco / Valli, Maria Beatrice

    Journal of Antimicrobial Chemotherapy

    2020  Volume 75, Issue 10, Page(s) 2977–2980

    Abstract: Abstract Background Remdesivir is a prodrug of the nucleoside analogue GS-441524 and is under evaluation for treatment of SARS-CoV-2-infected patients. Objectives To evaluate the pharmacokinetics of remdesivir and GS-441524 in plasma, bronchoalveolar ... ...

    Abstract Abstract Background Remdesivir is a prodrug of the nucleoside analogue GS-441524 and is under evaluation for treatment of SARS-CoV-2-infected patients. Objectives To evaluate the pharmacokinetics of remdesivir and GS-441524 in plasma, bronchoalveolar aspirate (BAS) and CSF in two critically ill COVID-19 patients. Methods Remdesivir was administered at 200 mg loading dose on the first day followed by 12 days of 100 mg in two critically ill patients. Blood samples were collected immediately after (C0) and at 1 (C1) and 24 h (C24) after intravenous administration on day 3 until day 9. BAS samples were collected on Days 4, 7 and 9 from both patients while one CSF on Day 7 was obtained in one patient. Remdesivir and GS-441524 concentrations were measured in these samples using a validated UHPLC-MS/MS method. Results We observed higher concentrations of remdesivir at C0 (6- to 7-fold higher than EC50 from in vitro studies) and a notable decay at C1. GS-441524 plasma concentrations reached a peak at C1 and persisted until the next administration. Higher concentrations of GS-441524 were observed in the patient with mild renal dysfunction. Mean BAS/plasma concentration ratios of GS-441524 were 2.3% and 6.4% in Patient 1 and Patient 2, respectively. The CSF concentration found in Patient 2 was 25.7% with respect to plasma. GS-441524 levels in lung and CNS suggest compartmental differences in drug exposure. Conclusions We report the first pharmacokinetic evaluation of remdesivir and GS-441524 in recovered COVID-19 patients. Further study of the pharmacokinetic profile of remdesivir, GS-441524 and the intracellular triphosphate form are required.
    Keywords Pharmacology (medical) ; Pharmacology ; Infectious Diseases ; covid19
    Language English
    Publisher Oxford University Press (OUP)
    Publishing country uk
    Document type Article ; Online
    ZDB-ID 191709-2
    ISSN 1460-2091 ; 0305-7453
    ISSN (online) 1460-2091
    ISSN 0305-7453
    DOI 10.1093/jac/dkaa239
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: Risk and predictive factors of prolonged viral RNA shedding in upper respiratory specimens in a large cohort of COVID-19 patients admitted to an Italian reference hospital.

    Mondi, Annalisa / Lorenzini, Patrizia / Castilletti, Concetta / Gagliardini, Roberta / Lalle, Eleonora / Corpolongo, Angela / Valli, Maria Beatrice / Taglietti, Fabrizio / Cicalini, Stefania / Loiacono, Laura / Di Gennaro, Francesco / D'Offizi, Gianpiero / Palmieri, Fabrizio / Nicastri, Emanuele / Agrati, Chiara / Petrosillo, Nicola / Ippolito, Giuseppe / Vaia, Francesco / Girardi, Enrico /
    Capobianchi, Maria Rosaria / Antinori, Andrea / Zito, Sara / Abbonizio, Maria Alessandra / Abdeddaim, Amina / Agostini, Elisabetta / Albarello, Fabrizio / Amadei, Gioia / Amendola, Alessandra / Antonica, Maria Assunta / Antonini, Mario / Bartoli, Tommaso Ascoli / Baldini, Francesco / Barbaro, Raffaella / Bartolini, Barbara / Bellagamba, Rita / Benigni, Martina / Bevilacqua, Nazario / Biava, Gianluigi / Bibas, Michele / Bordi, Licia / Bordoni, Veronica / Boumis, Evangelo / Branca, Marta / Buonomo, Rosanna / Busso, Donatella / Camici, Marta / Campioni, Paolo / Canichella, Flaminia / Capone, Alessandro / Caporale, Cinzia / Caraffa, Emanuela / Caravella, Ilaria / Carletti, Fabrizio / Cataldo, Adriana / Cerilli, Stefano / Cerva, Carlotta / Chiappini, Roberta / Chinello, Pierangelo / Cianfarani, Maria Assunta / Ciaralli, Carmine / Cimaglia, Claudia / Cinicola, Nicola / Ciotti, Veronica / Colavita, Francesca / Cristofaro, Massimo / Curiale, Salvatore / D'Abramo, Alessandra / Dantimi, Cristina / De Angelis, Alessia / De Angelis, Giada / De Palo, Maria Grazia / De Zottis, Federico / Di Bari, Virginia / Di Lorenzo, Rachele / Di Stefano, Federica / Donno, Davide / Evangelista, Francesca / Faraglia, Francesca / Farina, Anna / Ferraro, Federica / Fiorentini, Lorena / Frustaci, Andrea / Fusetti, Matteo / Galati, Vincenzo / Gallì, Paola / Garotto, Gabriele / Gaviano, Ilaria / Tekle, Saba Gebremeskel / Giancola, Maria Letizia / Giansante, Filippo / Giombini, Emanuela / Granata, Guido / Greci, Maria Cristina / Grilli, Elisabetta / Grisetti, Susanna / Gualano, Gina / Iacomi, Fabio / Iaconi, Marta / Iannicelli, Giuseppina / Inversi, Carlo / Lamanna, Maria Elena / Lanini, Simone / Lapa, Daniele / Lepore, Luciana / Libertone, Raffaella / Lionetti, Raffaella / Liuzzi, Giuseppina / Lucia, Andrea / Lufrani, Franco / Macchione, Manuela / Maffongelli, Gaetano / Marani, Alessandra / Marchioni, Luisa / Mariano, Andrea / Marini, Maria Cristina / Maritti, Micaela / Mastrobattista, Annelisa / Mastrorosa, Ilaria / Matusali, Giulia / Mazzotta, Valentina / Mencarini, Paola / Meschi, Silvia / Messina, Francesco / Micarelli, Sibiana / Mogavero, Giulia / Montalbano, Marzia / Montaldo, Chiara / Mosti, Silvia / Murachelli, Silvia / Musso, Maria / Nardi, Michela / Navarra, Assunta / Nocioni, Martina / Noto, Pasquale / Noto, Roberto / Oliva, Alessandra / Onnis, Ilaria / Ottou, Sandrine / Palazzolo, Claudia / Pallini, Emanuele / Palombi, Giulio / Pareo, Carlo / Passeri, Virgilio / Pelliccioni, Federico / Penna, Giovanna / Petrecchia, Antonella / Petrone, Ada / Pianura, Elisa / Pinnetti, Carmela / Pisciotta, Maria / Piselli, Pierluca / Pittalis, Silvia / Pontarelli, Agostina / Proietti, Costanza / Puro, Vincenzo / Ramazzini, Paolo Migliorisi / Rianda, Alessia / Rinonapoli, Gabriele / Rosati, Silvia / Rubino, Dorotea / Rueca, Martina / Ruggeri, Alberto / Sacchi, Alessandra / Sampaolesi, Alessandro / Sanasi, Francesco / Santagata, Carmen / Scarabello, Alessandra / Scarcia, Silvana / Schininà, Vincenzo / Scognamiglio, Paola / Scorzolini, Laura / Stazi, Giulia / Strano, Giacomo / Taibi, Chiara / Taloni, Giorgia / Nardi, Tetaj / Tonnarini, Roberto / Topino, Simone / Tozzi, Martina / Vairo, Francesco / Vergori, Alessandra / Vincenzi, Laura / Visco-Comandini, Ubaldo / Vita, Serena / Vittozzi, Pietro / Zaccarelli, Mauro / Zanetti, Antonella

    International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases

    2021  Volume 105, Page(s) 532–539

    Abstract: Background: Limited data are available about the predictors and outcomes associated with prolonged SARS-CoV-2 RNA shedding (VS).: Methods: A retrospective study including COVID-19 patients admitted to an Italian hospital between March 1 and July 1, ... ...

    Abstract Background: Limited data are available about the predictors and outcomes associated with prolonged SARS-CoV-2 RNA shedding (VS).
    Methods: A retrospective study including COVID-19 patients admitted to an Italian hospital between March 1 and July 1, 2020. Predictors of viral clearance (VC) and prolonged VS from the upper respiratory tract were assessed by Poisson regression and logistic regression analyses. The causal relation between VS and clinical outcomes was evaluated through an inverse probability weighted Cox model.
    Results: The study included 536 subjects. The median duration of VS from symptoms onset was 18 days. The estimated 30-day probability of VC was 70.2%. Patients with comorbidities, lymphopenia at hospital admission, or moderate/severe respiratory disease had a lower chance of VC. The development of moderate/severe respiratory failure, delayed hospital admission after symptoms onset, baseline comorbidities, or D-dimer >1000ng/mL at admission independently predicted prolonged VS. The achievement of VC doubled the chance of clinical recovery and reduced the probability of death/mechanical ventilation.
    Conclusions: Respiratory disease severity, comorbidities, delayed hospital admission and inflammatory markers negatively predicted VC, which resulted to be associated with better clinical outcomes. These findings highlight the importance of prompt hospitalization of symptomatic patients, especially where signs of severity or comorbidities are present.
    MeSH term(s) Adult ; Aged ; COVID-19/virology ; Cohort Studies ; Female ; Humans ; Male ; Middle Aged ; Proportional Hazards Models ; RNA, Viral/analysis ; Respiratory System/virology ; SARS-CoV-2/isolation & purification ; Severity of Illness Index ; Time Factors ; Virus Shedding
    Chemical Substances RNA, Viral
    Language English
    Publishing date 2021-03-03
    Publishing country Canada
    Document type Journal Article
    ZDB-ID 1331197-9
    ISSN 1878-3511 ; 1201-9712
    ISSN (online) 1878-3511
    ISSN 1201-9712
    DOI 10.1016/j.ijid.2021.02.117
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Virological Characterization of the First 2 COVID-19 Patients Diagnosed in Italy

    Colavita, Francesca / Lapa, Daniele / Carletti, Fabrizio / Lalle, Eleonora / Messina, Francesco / Rueca, Martina / Matusali, Giulia / Meschi, Silvia / Bordi, Licia / Marsella, Patrizia / Nicastri, Emanuele / Marchioni, Luisa / Mariano, Andrea / Scorzolini, Laura / Ascoli Bartoli, Tommaso / Di Caro, Antonino / Ippolito, Giuseppe / Capobianchi, Maria Rosaria / Castilletti, Concetta /
    Abbate, Isabella / Agrati, Chiara / Aleo, Loredana / Alonzi, Tonino / Amendola, Alessandra / Apollonio, Claudia / Arduini, Nicolina / Bartolini, Barbara / Berno, Giulia / Biancone, Silvia / Biava, Mirella / Bibbò, Angela / Brega, Carla / Canali, Marco / Cannas, Angela / Carrara, Stefania / Casetti, Rita / Chiappini, Roberta / Ciafrone, Lucia / Cimini, Eleonora / Coen, Sabrina / Condello, Rossella / Coppola, Antonio / D’Arezzo, Silvia / Di Filippo, Stefania / Di Giuli, Chiara / Fabeni, Lavinia / Felici, Luisa / Ferraioli, Valeria / Forbici, Federica / Garbuglia, Anna Rosa / Giombini, Emanuela / Gori, Caterina / Graziano, Silvia / Gruber, Cesare Ernesto Maria / Khouri, Daniele / Leone, Barbara / Massimino, Chiara / Mazzarelli, Antonio / Minosse, Claudia / Montaldo, Claudia / Neri, Stefania / Nisii, Carla / Petrivelli, Elisabetta / Petroni, Fabrizio / Petruccioli, Elisa / Pisciotta, Marina / Pizzi, Daniele / Prota, Gianluca / Raparelli, Fabrizio / Rozera, Gabriella / Sabatini, Rossella / Sarti, Silvia / Sberna, Giuseppe / Sciamanna, Roberta / Selleri, Marina / Selvaggi, Carla / Sias, Catia / Stellitano, Chiara / Toffoletti, Antonietta / Truffa, Silvia / Turchi, Federica / Valli, Maria Beatrice / Venditti, Carolina / Vescovo, Tiziana / Vincenti, Donatella / Vulcano, Antonella / Zambelli, Emma / Abbonizio, Maria Alessandra / Albarello, Fabrizio / Amadei, Gioia / Antonini, Mario / Barbaro, Raffaella / Benigni, Martina / Bevilacqua, Nazario / Bordoni, Veronica / Branca, Marta / Campioni, Paolo / Caporale, Cinzia / Caravella, Ilaria / Ciaralli, Carmine / Corpolongo, Angela / Cristofaro, Massimo / Curiale, Salvatore / D’Abramo, Alessandra / Dantimi, Cristina / De Angelis, Alessia / De Angelis, Giada / Di Lorenzo, Rachele / Di Stefano, Federica / Ferraro, Federica / Fiorentini, Lorena / Frustaci, Andrea / Gallì, Paola / Garotto, Gabriele / Giancola, Maria Letizia / Giansante, Filippo / Greci, Maria Cristina / Lanini, Simone / Lepore, Luciana / Lucia, Andrea / Lufrani, Franco / Macchione, Manuela / Marani, Alessandra / Marini, Maria Cristina / Maritti, Micaela / Montaldo, Chiara / Murachelli, Silvia / Noto, Roberto / Palazzolo, Claudia / Pallini, Emanuele / Passeri, Virgilio / Pelliccioni, Federico / Petrecchia, Antonella / Petrone, Ada / Petrosillo, Nicola / Pianura, Elisa / Pisciotta, Maria / Pittalis, Silvia / Proietti, Costanza / Puro, Vincenzo / Rinonapoli, Gabriele / Sacchi, Alessandra / Sanasi, Francesco / Santagata, Carmen / Scarcia, Silvana / Schininà, Vincenzo / Scognamiglio, Paola / Stazi, Giulia / Vaia, Francesco / Vairo, Francesco

    Open Forum Infectious Diseases

    Phylogenetic Analysis, Virus Shedding Profile From Different Body Sites, and Antibody Response Kinetics

    2020  Volume 7, Issue 10

    Abstract: Abstract Background The pathogenesis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection remains unclear. We report the detection of viral RNA from different anatomical districts and the antibody profile in the first 2 COVID-19 ... ...

    Abstract Abstract Background The pathogenesis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection remains unclear. We report the detection of viral RNA from different anatomical districts and the antibody profile in the first 2 COVID-19 cases diagnosed in Italy. Methods We tested for SARS-CoV-2 RNA clinical samples, either respiratory and nonrespiratory (ie, saliva, serum, urine, vomit, rectal, ocular, cutaneous, and cervico-vaginal swabs), longitudinally collected from both patients throughout the hospitalization. Serological analysis was carried out on serial serum samples to evaluate IgM, IgA, IgG, and neutralizing antibody levels. Results SARS-CoV-2 RNA was detected since the early phase of illness, lasting over 2 weeks in both upper and lower respiratory tract samples. Virus isolate was obtained from acute respiratory samples, while no infectious virus was rescued from late respiratory samples with low viral RNA load, collected when serum antibodies had been developed. Several other specimens came back positive, including saliva, vomit, rectal, cutaneous, cervico-vaginal, and ocular swabs. IgM, IgA, and IgG were detected within the first week of diagnosis, with IgG appearing earlier and at higher titers. Neutralizing antibodies developed during the second week, reaching high titers 32 days after diagnosis. Conclusions Our longitudinal analysis showed that SARS-CoV-2 RNA can be detected in different body samples, which may be associated with broad tropism and different spectra of clinical manifestations and modes of transmission. Profiling antibody response and neutralizing activity can assist in laboratory diagnosis and surveillance actions.
    Keywords Oncology ; Clinical Neurology ; covid19
    Language English
    Publisher Oxford University Press (OUP)
    Publishing country uk
    Document type Article ; Online
    ZDB-ID 2757767-3
    ISSN 2328-8957
    ISSN 2328-8957
    DOI 10.1093/ofid/ofaa403
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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