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Article ; Online: Induction of Human T Cell Development In Vitro with OP9-DL4-7FS Cells Expressing Human Cytokines.

Mohtashami, Mahmood / Brauer, Patrick M / Zúñiga-Pflücker, Juan Carlos

Methods in molecular biology (Clifton, N.J.)

2022 Volume 2580, Page(s) 249–260

Abstract: For nearly a generation now, OP9-DL1 and OP9-DL4 cells have provided an efficient and reliable cell system to generate T cells from mouse and human hematopoietic stem cells (HSCs) and pluripotent stem cells. OP9-DL1 and OP9-DL4 were originally derived ... ...

Abstract	For nearly a generation now, OP9-DL1 and OP9-DL4 cells have provided an efficient and reliable cell system to generate T cells from mouse and human hematopoietic stem cells (HSCs) and pluripotent stem cells. OP9-DL1 and OP9-DL4 were originally derived from the OP9 mouse bone marrow stromal cell line, which was transduced to ectopically express Delta-like 1 or 4 proteins, respectively. OP9-DL cells mimic the thymic microenvironment in that when cocultured with mouse or human (h) HSCs, they interact with and activate Notch receptors present on HSCs, required for T cell differentiation. The HSC/OP9-DL cocultures require additional cytokines that are necessary for survival and proliferation of hematopoietic cells. For hHSCs, these factors are interleukin-7 (IL-7), stem cell factor (SCF), and FMS-like tyrosine kinase 3 ligand (FLT3L) that are normally exogenously added to the cocultures. In this chapter, we describe methods for establishing a novel and improved version of OP9-DL4 cells, called OP9-DL4-7FS cells that circumvent the addition of these costly cytokines, by transducing OP9-DL4 cell line to express human IL-7, FLT3L, and SCF (7FS). Herein, we describe the protocol for the generation of OP9-DL4-7FS cells and the conditions for OP9-DL4-7FS/hHSC coculture to support T cell lineage initiation and expansion while comparing it to the now "classic" OP9-DL4 coculture. The use of OP9-DL4-7FS cell system will provide an improved and cost-effective method to the commonly used OP9-DL/HSC coculture for studying both mouse and human T cell development.
MeSH term(s)	Humans ; Mice ; Animals ; Interleukin-7/metabolism ; Cytokines/metabolism ; Cell Differentiation ; Hematopoietic Stem Cells ; Coculture Techniques ; T-Lymphocytes ; Stromal Cells/metabolism
Chemical Substances	Interleukin-7 ; Cytokines
Language	English
Publishing date	2022-11-14
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1940-6029
ISSN (online)	1940-6029
DOI	10.1007/978-1-0716-2740-2_15
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Article ; Online: Cutting Edge: TCR-β Selection Is Required at the CD4

Chen, Edward L Y / Brauer, Patrick M / Martinez, Elisa C / Huang, Xiaotian / Yu, Ning / Anderson, Michele K / Li, Yang / Zúñiga-Pflücker, Juan Carlos

Journal of immunology (Baltimore, Md. : 1950)

2021 Volume 206, Issue 10, Page(s) 2271–2276

Abstract: T cell development is predicated on the successful rearrangement of the TCR gene loci, which encode for Ag-specific receptors. Recombination-activating gene (RAG) 2 is required for TCR gene rearrangements, which occur during specific stages of T cell ... ...

Abstract	T cell development is predicated on the successful rearrangement of the TCR gene loci, which encode for Ag-specific receptors. Recombination-activating gene (RAG) 2 is required for TCR gene rearrangements, which occur during specific stages of T cell development. In this study, we differentiated human pluripotent stem cells with a CRISPR/Cas9-directed deletion of the
MeSH term(s)	Animals ; CD4 Antigens/metabolism ; CD8 Antigens/metabolism ; Cell Differentiation/genetics ; Cell Line, Tumor ; DNA-Binding Proteins/deficiency ; DNA-Binding Proteins/genetics ; Gene Knockout Techniques ; Gene Rearrangement, beta-Chain T-Cell Antigen Receptor/genetics ; Hematopoiesis/genetics ; Human Embryonic Stem Cells/cytology ; Humans ; Lymphocyte Activation/genetics ; Mice ; Mice, Inbred NOD ; Mice, SCID ; Nuclear Proteins/deficiency ; Nuclear Proteins/genetics ; Receptors, Antigen, T-Cell, alpha-beta/genetics ; Receptors, Antigen, T-Cell, alpha-beta/metabolism ; T-Lymphocytes/immunology ; Transduction, Genetic
Chemical Substances	CD4 Antigens ; CD8 Antigens ; DNA-Binding Proteins ; Nuclear Proteins ; RAG2 protein, human ; Receptors, Antigen, T-Cell, alpha-beta
Language	English
Publishing date	2021-05-03
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural
ZDB-ID	3056-9
ISSN	1550-6606 ; 0022-1767 ; 1048-3233 ; 1047-7381
ISSN (online)	1550-6606
ISSN	0022-1767 ; 1048-3233 ; 1047-7381
DOI	10.4049/jimmunol.2100141
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Article ; Online: T Cell Genesis: In Vitro Veritas Est?

Brauer, Patrick M / Singh, Jastaranpreet / Xhiku, Sintia / Zúñiga-Pflücker, Juan Carlos

Trends in immunology

2016 Volume 37, Issue 12, Page(s) 889–901

Abstract: T cells, as orchestrators of the adaptive immune response, serve important physiological and potentially therapeutic roles, for example in cancer immunotherapy. T cells are readily isolated from patients; however, the yield of antigen-specific T cells is ...

Abstract	T cells, as orchestrators of the adaptive immune response, serve important physiological and potentially therapeutic roles, for example in cancer immunotherapy. T cells are readily isolated from patients; however, the yield of antigen-specific T cells is limited, thus making their clinical use challenging. Therefore, the generation of T lymphocytes from hematopoietic stem/progenitor cells (HSPCs) and human pluripotent stem cells (PSCs) in vitro provides an attractive method for the large-scale production and genetic manipulation of T cells. In this review, we discuss recent strategies for the generation of T cells from human HSPCs and PSCs in vitro. Continued advancement in the generation of human T cells in vitro will expand their benefits and therapeutic potential in the clinic.
MeSH term(s)	Animals ; Cancer Vaccines/immunology ; Cell Differentiation ; Cell Proliferation ; Cell Survival ; Genetic Engineering ; Hematopoietic Stem Cells/physiology ; Humans ; Immunotherapy, Adoptive/methods ; In Vitro Techniques ; Lymphopoiesis ; Neoplasms/immunology ; Neoplasms/therapy ; Organ Culture Techniques ; Pluripotent Stem Cells/physiology ; T-Lymphocytes/physiology ; T-Lymphocytes/transplantation
Chemical Substances	Cancer Vaccines
Language	English
Publishing date	2016-12
Publishing country	England
Document type	Journal Article ; Review
ZDB-ID	2036831-8
ISSN	1471-4981 ; 1471-4906
ISSN (online)	1471-4981
ISSN	1471-4906
DOI	10.1016/j.it.2016.09.008
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Article: Building a better understanding of the intracellular tyrosine kinase PTK6 - BRK by BRK.

Brauer, Patrick M / Tyner, Angela L

Biochimica et biophysica acta

2010 Volume 1806, Issue 1, Page(s) 66–73

Abstract: Protein tyrosine kinase 6 (PTK6), also referred to as breast tumor kinase BRK, is a member of a distinct family of kinases that is evolutionarily related to the SRC family of tyrosine kinases. While not expressed in the normal mammary gland, PTK6 ... ...

Abstract	Protein tyrosine kinase 6 (PTK6), also referred to as breast tumor kinase BRK, is a member of a distinct family of kinases that is evolutionarily related to the SRC family of tyrosine kinases. While not expressed in the normal mammary gland, PTK6 expression is detected in a large proportion of human mammary gland tumors. In breast tumor cells, PTK6 promotes growth factor signaling and cell migration. PTK6 expression is also increased in a number of other epithelial tumors, including ovarian and colon cancer. In contrast, PTK6 is expressed in diverse normal epithelia, including the linings of the gastrointestinal tract, skin and prostate, where its expression correlates with cell cycle exit and differentiation. Disruption of the mouse Ptk6 gene leads to increased growth and impaired differentiation in the small intestine that is accompanied by increased AKT and Wnt signaling. Following total body irradiation, PTK6 expression is induced in proliferating progenitor cells of the intestine, where it plays an essential role in DNA-damage induced apoptosis. A distinguishing feature of PTK6 is its flexibility in intracellular localization, due to a lack of amino-terminal myristoylation/palmitoylation. Recently a number of substrates of PTK6 have been identified, including nuclear RNA-binding proteins and transcription factors. We discuss PTK6 signaling, its apparent conflicting roles in cancer and normal epithelia, and its potential as a therapeutic target in epithelial cancers.
MeSH term(s)	Animals ; Humans ; Neoplasm Proteins/chemistry ; Neoplasm Proteins/genetics ; Neoplasm Proteins/physiology ; Neoplasms/drug therapy ; Neoplasms/etiology ; Protein-Tyrosine Kinases/chemistry ; Protein-Tyrosine Kinases/genetics ; Protein-Tyrosine Kinases/physiology ; Signal Transduction
Chemical Substances	Neoplasm Proteins ; Protein-Tyrosine Kinases (EC 2.7.10.1) ; PTK6 protein, human (EC 2.7.10.2)
Language	English
Publishing date	2010-02-26
Publishing country	Netherlands
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S. ; Review
ZDB-ID	60-7
ISSN	1879-2596 ; 1879-260X ; 1872-8006 ; 1879-2642 ; 1879-2618 ; 1879-2650 ; 0006-3002 ; 0005-2728 ; 0005-2736 ; 0304-4165 ; 0167-4838 ; 1388-1981 ; 0167-4889 ; 0167-4781 ; 0304-419X ; 1570-9639 ; 0925-4439 ; 1874-9399
ISSN (online)	1879-2596 ; 1879-260X ; 1872-8006 ; 1879-2642 ; 1879-2618 ; 1879-2650
ISSN	0006-3002 ; 0005-2728 ; 0005-2736 ; 0304-4165 ; 0167-4838 ; 1388-1981 ; 0167-4889 ; 0167-4781 ; 0304-419X ; 1570-9639 ; 0925-4439 ; 1874-9399
DOI	10.1016/j.bbcan.2010.02.003
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Article ; Online: RAKing in AKT: a tumor suppressor function for the intracellular tyrosine kinase FRK.

Brauer, Patrick M / Tyner, Angela L

Cell cycle (Georgetown, Tex.)

2009 Volume 8, Issue 17, Page(s) 2728–2732

Abstract: The Fyn related kinase FRK, originally called RAK, is a member of a small family of intracellular Src-related tyrosine kinases that includes PTK6 and Srms. These kinases share a conserved gene structure that is distinct from that of the Src family. ... ...

Abstract	The Fyn related kinase FRK, originally called RAK, is a member of a small family of intracellular Src-related tyrosine kinases that includes PTK6 and Srms. These kinases share a conserved gene structure that is distinct from that of the Src family. Expression of FRK and PTK6 was originally identified in melanoma, breast cancer cells and normal intestinal epithelium, and both FRK and PTK6 have been implicated in the regulation of epithelial cell differentiation and apoptosis. Recently FRK was reported to phosphorylate the tumor suppressor PTEN (phosphatase and tensin homolog deleted from chromosome 10), a negative regulator of phosphatidylinositol 3 kinase (PI3K) signaling and AKT activation. FRK-mediated tyrosine phosphorylation of PTEN suppressed its association with NEDD4-1, an E3 ubiquitin ligase that may target it for polyubiquitination and proteosomal degradation. As a positive regulator of PTEN, FRK suppresses AKT signaling and inhibits breast cancer cell tumorgenicity in xenograft models. Both FRK and the related tyrosine kinase PTK6 appear to have multiple context-dependent functions, including the ability to regulate AKT. Although PTK6 negatively regulates AKT signaling in normal tissues in vivo, it may enhance AKT signaling in breast cancer cells. In contrast, FRK, which is expressed in the normal mammary gland but lost in some breast tumors, has tumor suppressor functions in mammary gland cells.
MeSH term(s)	Apoptosis ; Cell Differentiation ; Endosomal Sorting Complexes Required for Transport/metabolism ; Humans ; Nedd4 Ubiquitin Protein Ligases ; Neoplasm Proteins/metabolism ; PTEN Phosphohydrolase/metabolism ; Phosphatidylinositol 3-Kinases/metabolism ; Protein-Tyrosine Kinases/metabolism ; Proto-Oncogene Proteins c-akt/metabolism ; Tumor Suppressor Proteins/metabolism ; Ubiquitin-Protein Ligases/metabolism
Chemical Substances	Endosomal Sorting Complexes Required for Transport ; Neoplasm Proteins ; Tumor Suppressor Proteins ; Nedd4 Ubiquitin Protein Ligases (EC 2.3.2.26) ; Nedd4 protein, human (EC 2.3.2.26) ; Ubiquitin-Protein Ligases (EC 2.3.2.27) ; Phosphatidylinositol 3-Kinases (EC 2.7.1.-) ; Protein-Tyrosine Kinases (EC 2.7.10.1) ; FRK protein, human (EC 2.7.10.2) ; PTK6 protein, human (EC 2.7.10.2) ; Proto-Oncogene Proteins c-akt (EC 2.7.11.1) ; PTEN Phosphohydrolase (EC 3.1.3.67) ; PTEN protein, human (EC 3.1.3.67)
Language	English
Publishing date	2009-09-29
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Review
ZDB-ID	2146183-1
ISSN	1551-4005 ; 1538-4101 ; 1554-8627
ISSN (online)	1551-4005
ISSN	1538-4101 ; 1554-8627
DOI	10.4161/cc.8.17.9389
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Article ; Online: DL4-μbeads induce T cell lineage differentiation from stem cells in a stromal cell-free system.

Trotman-Grant, Ashton C / Mohtashami, Mahmood / De Sousa Casal, Joshua / Martinez, Elisa C / Lee, Dylan / Teichman, Sintia / Brauer, Patrick M / Han, Jianxun / Anderson, Michele K / Zúñiga-Pflücker, Juan Carlos

Nature communications

2021 Volume 12, Issue 1, Page(s) 5023

Abstract: T cells are pivotal effectors of the immune system and can be harnessed as therapeutics for regenerative medicine and cancer immunotherapy. An unmet challenge in the field is the development of a clinically relevant system that is readily scalable to ... ...

Abstract	T cells are pivotal effectors of the immune system and can be harnessed as therapeutics for regenerative medicine and cancer immunotherapy. An unmet challenge in the field is the development of a clinically relevant system that is readily scalable to generate large numbers of T-lineage cells from hematopoietic stem/progenitor cells (HSPCs). Here, we report a stromal cell-free, microbead-based approach that supports the efficient in vitro development of both human progenitor T (proT) cells and T-lineage cells from CD34
MeSH term(s)	Adaptor Proteins, Signal Transducing/genetics ; Adaptor Proteins, Signal Transducing/immunology ; Animals ; Antigens, CD34/genetics ; Antigens, CD34/immunology ; Calcium-Binding Proteins/genetics ; Calcium-Binding Proteins/immunology ; Cell Lineage ; Cell- and Tissue-Based Therapy ; Cells, Cultured ; Hematopoietic Stem Cell Transplantation ; Hematopoietic Stem Cells/cytology ; Hematopoietic Stem Cells/immunology ; Humans ; Lymphopoiesis ; Mice ; Mice, Inbred C57BL ; Mice, Inbred NOD ; Pluripotent Stem Cells/cytology ; Pluripotent Stem Cells/immunology ; Primary Immunodeficiency Diseases/genetics ; Primary Immunodeficiency Diseases/immunology ; Primary Immunodeficiency Diseases/physiopathology ; Primary Immunodeficiency Diseases/therapy ; T-Lymphocytes/cytology ; T-Lymphocytes/immunology ; T-Lymphocytes/transplantation
Chemical Substances	Adaptor Proteins, Signal Transducing ; Antigens, CD34 ; Calcium-Binding Proteins ; DLL4 protein, human
Language	English
Publishing date	2021-08-18
Publishing country	England
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	2553671-0
ISSN	2041-1723 ; 2041-1723
ISSN (online)	2041-1723
ISSN	2041-1723
DOI	10.1038/s41467-021-25245-8
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Article ; Online: Targeted Disruption of TCF12 Reveals HEB as Essential in Human Mesodermal Specification and Hematopoiesis.

Li, Yang / Brauer, Patrick M / Singh, Jastaranpreet / Xhiku, Sintia / Yoganathan, Kogulan / Zúñiga-Pflücker, Juan Carlos / Anderson, Michele K

Stem cell reports

2017 Volume 9, Issue 3, Page(s) 779–795

Abstract: Hematopoietic stem cells arise from mesoderm-derived hemogenic endothelium (HE) during embryogenesis in a process termed endothelial-hematopoietic transition (EHT). To better understand the gene networks that control this process, we investigated the ... ...

Abstract	Hematopoietic stem cells arise from mesoderm-derived hemogenic endothelium (HE) during embryogenesis in a process termed endothelial-hematopoietic transition (EHT). To better understand the gene networks that control this process, we investigated the role of the transcription factor HEB (TCF12) by disrupting the TCF12 gene locus in human embryonic stem cells (hESCs) and inducing them to differentiate toward hematopoietic outcomes. HEB-deficient hESCs retained key features of pluripotency, including expression of SOX2 and SSEA-4 and teratoma formation, while NANOG expression was reduced. Differentiation of HEB
Language	English
Publishing date	2017-09-12
Publishing country	United States
Document type	Journal Article
ZDB-ID	2720528-9
ISSN	2213-6711 ; 2213-6711
ISSN (online)	2213-6711
ISSN	2213-6711
DOI	10.1016/j.stemcr.2017.07.011
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Article ; Online: Engineering the haemogenic niche mitigates endogenous inhibitory signals and controls pluripotent stem cell-derived blood emergence.

Rahman, Nafees / Brauer, Patrick M / Ho, Lilian / Usenko, Tatiana / Tewary, Mukul / Zúñiga-Pflücker, Juan Carlos / Zandstra, Peter W

Nature communications

2017 Volume 8, Page(s) 15380

Abstract: Efforts to recapitulate haematopoiesis, a process guided by spatial and temporal inductive signals, to generate haematopoietic progenitors from human pluripotent stem cells (hPSCs) have focused primarily on exogenous signalling pathway activation or ... ...

Abstract	Efforts to recapitulate haematopoiesis, a process guided by spatial and temporal inductive signals, to generate haematopoietic progenitors from human pluripotent stem cells (hPSCs) have focused primarily on exogenous signalling pathway activation or inhibition. Here we show haemogenic niches can be engineered using microfabrication strategies by micropatterning hPSC-derived haemogenic endothelial (HE) cells into spatially-organized, size-controlled colonies. CD34+VECAD+ HE cells were generated with multi-lineage potential in serum-free conditions and cultured as size-specific haemogenic niches that displayed enhanced blood cell induction over non-micropatterned cultures. Intra-colony analysis revealed radial organization of CD34 and VECAD expression levels, with CD45+ blood cells emerging primarily from the colony centroid area. We identify the induced interferon gamma protein (IP-10)/p-38 MAPK signalling pathway as the mechanism for haematopoietic inhibition in our culture system. Our results highlight the role of spatial organization in hPSC-derived blood generation, and provide a quantitative platform for interrogating molecular pathways that regulate human haematopoiesis.
MeSH term(s)	Animals ; Antigens, CD/metabolism ; Antigens, CD34/metabolism ; Cadherins/metabolism ; Cell Differentiation ; Cell Engineering/methods ; Cell Line ; Cell Lineage ; Chemokine CXCL10/metabolism ; Culture Media, Serum-Free ; Female ; Hemangioblasts/cytology ; Hemangioblasts/metabolism ; Hemangioblasts/transplantation ; Hematopoiesis ; Hematopoietic Stem Cell Transplantation ; Hematopoietic Stem Cells/cytology ; Hematopoietic Stem Cells/metabolism ; Heterografts ; Humans ; Leukocyte Common Antigens/metabolism ; Mice ; Pluripotent Stem Cells/cytology ; Pluripotent Stem Cells/metabolism ; Pluripotent Stem Cells/transplantation ; Signal Transduction ; Stem Cell Niche
Chemical Substances	Antigens, CD ; Antigens, CD34 ; CXCL10 protein, human ; Cadherins ; Chemokine CXCL10 ; Culture Media, Serum-Free ; cadherin 5 ; Leukocyte Common Antigens (EC 3.1.3.48) ; PTPRC protein, human (EC 3.1.3.48)
Language	English
Publishing date	2017-05-25
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2553671-0
ISSN	2041-1723 ; 2041-1723
ISSN (online)	2041-1723
ISSN	2041-1723
DOI	10.1038/ncomms15380
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Article ; Online: Cytoplasmic retention of protein tyrosine kinase 6 promotes growth of prostate tumor cells.

Brauer, Patrick M / Zheng, Yu / Wang, Lin / Tyner, Angela L

Cell cycle (Georgetown, Tex.)

2010 Volume 9, Issue 20, Page(s) 4190–4199

Abstract: Protein tyrosine kinase 6 (PTK6) is an intracellular tyrosine kinase that is nuclear in epithelial cells of the normal prostate, but cytoplasmic in prostate tumors and in the PC3 prostate tumor cell line. The impact of altered PTK6 intracellular ... ...

Abstract	Protein tyrosine kinase 6 (PTK6) is an intracellular tyrosine kinase that is nuclear in epithelial cells of the normal prostate, but cytoplasmic in prostate tumors and in the PC3 prostate tumor cell line. The impact of altered PTK6 intracellular localization in prostate tumor cells has not been extensively explored. Knockdown of endogenous cytoplasmic PTK6 resulted in decreased PC3 cell proliferation and colony formation, suggesting that cytoplasmic PTK6 stimulates oncogenic pathways. In contrast, reintroduction of PTK6 into nuclei of PC3 cells had a negative effect on growth. Enhanced tyrosine phosphorylation of the PTK6 substrate Sam68 was detected in cells expressing nuclear-targeted PTK6. We found that mechanisms regulating nuclear localization of PTK6 are intact in PC3 cells. Transiently overexpressed PTK6 readily enters the nucleus. Ectopic expression of ALT-PTK6, a catalytically inactive splice variant of PTK6, did not affect localization of endogenous PTK6 in PC3 cells. Using leptomycin B, we confirmed that cytoplasmic localization of endogenous PTK6 is not due to Crm-1/exportin-1 mediated nuclear export. In addition, overexpression of the PTK6 nuclear substrate Sam68 is not sufficient to bring PTK6 into the nucleus. While exogenous PTK6 was readily detected in the nucleus when transiently expressed at high levels, low-level expression of inducible wild type PTK6 in stable cell lines resulted in its cytoplasmic retention. Our results suggest that retention of PTK6 in the cytoplasm of prostate cancer cells disrupts its ability to regulate nuclear substrates and leads to aberrant growth. In prostate cancer, restoring PTK6 nuclear localization may have therapeutic advantages.
MeSH term(s)	Active Transport, Cell Nucleus/physiology ; Adaptor Proteins, Signal Transducing/genetics ; Adaptor Proteins, Signal Transducing/metabolism ; Cell Line, Tumor ; Cell Nucleus/metabolism ; Cytoplasm/enzymology ; DNA-Binding Proteins/genetics ; DNA-Binding Proteins/metabolism ; Gene Knockdown Techniques ; Humans ; Karyopherins/metabolism ; Male ; Neoplasm Proteins/genetics ; Neoplasm Proteins/metabolism ; Nuclear Localization Signals ; Prostatic Neoplasms/enzymology ; Prostatic Neoplasms/pathology ; Protein-Tyrosine Kinases/genetics ; Protein-Tyrosine Kinases/metabolism ; RNA-Binding Proteins/genetics ; RNA-Binding Proteins/metabolism ; Receptors, Cytoplasmic and Nuclear/metabolism ; Signal Transduction/physiology ; Exportin 1 Protein
Chemical Substances	Adaptor Proteins, Signal Transducing ; DNA-Binding Proteins ; KHDRBS1 protein, human ; Karyopherins ; Neoplasm Proteins ; Nuclear Localization Signals ; RNA-Binding Proteins ; Receptors, Cytoplasmic and Nuclear ; Protein-Tyrosine Kinases (EC 2.7.10.1) ; PTK6 protein, human (EC 2.7.10.2)
Language	English
Publishing date	2010-10-30
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	2146183-1
ISSN	1551-4005 ; 1538-4101 ; 1554-8627
ISSN (online)	1551-4005
ISSN	1538-4101 ; 1554-8627
DOI	10.4161/cc.9.20.13518
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Article ; Online: The ion channel TRPM7 is required for B cell lymphopoiesis.

Krishnamoorthy, Mithunah / Buhari, Fathima Hifza Mohamed / Zhao, Tiantian / Brauer, Patrick M / Burrows, Kyle / Cao, Eric Yixiao / Moxley-Paquette, Vincent / Mortha, Arthur / Zúñiga-Pflücker, Juan Carlos / Treanor, Bebhinn

Science signaling

2018 Volume 11, Issue 533

Abstract: The transient receptor potential (TRP) family is a large family of widely expressed ion channels that regulate the intracellular concentration of ions and metals and respond to various chemical and physical stimuli. TRP subfamily M member 7 (TRPM7) is ... ...

Abstract	The transient receptor potential (TRP) family is a large family of widely expressed ion channels that regulate the intracellular concentration of ions and metals and respond to various chemical and physical stimuli. TRP subfamily M member 7 (TRPM7) is unusual in that it contains both an ion channel and a kinase domain. TRPM7 is a divalent cation channel with preference for Ca
MeSH term(s)	Animals ; B-Lymphocytes/cytology ; B-Lymphocytes/physiology ; Cells, Cultured ; Female ; Lymphopoiesis ; Magnesium/metabolism ; Mice ; Mice, Inbred C57BL ; Myeloid Cells/cytology ; Myeloid Cells/physiology ; TRPM Cation Channels/physiology
Chemical Substances	TRPM Cation Channels ; Trpm7 protein, mouse (EC 2.7.1.-) ; Magnesium (I38ZP9992A)
Language	English
Publishing date	2018-06-05
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2417226-1
ISSN	1937-9145 ; 1945-0877
ISSN (online)	1937-9145
ISSN	1945-0877
DOI	10.1126/scisignal.aan2693
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