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Article ; Online: Repair Process Impairment by

Ruffin, Manon / Brochiero, Emmanuelle

Frontiers in cellular and infection microbiology

2019 Volume 9, Page(s) 182

Abstract: Epithelial tissues protecting organs from the environment are the first-line of defense against pathogens. Therefore, efficient repair mechanisms after injury are crucial to maintain epithelial integrity. However, these healing processes can be ... ...

Abstract	Epithelial tissues protecting organs from the environment are the first-line of defense against pathogens. Therefore, efficient repair mechanisms after injury are crucial to maintain epithelial integrity. However, these healing processes can be insufficient to restore epithelial integrity, notably in infectious conditions.
MeSH term(s)	Alveolar Epithelial Cells ; Animals ; Drug Resistance, Multiple, Bacterial ; Epithelium/injuries ; Epithelium/microbiology ; Epithelium/physiology ; Epithelium, Corneal ; Humans ; Incidence ; Pseudomonas Infections/microbiology ; Pseudomonas Infections/therapy ; Pseudomonas aeruginosa/isolation & purification ; Pseudomonas aeruginosa/physiology ; Respiratory Mucosa
Language	English
Publishing date	2019-05-31
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	2619676-1
ISSN	2235-2988 ; 2235-2988
ISSN (online)	2235-2988
ISSN	2235-2988
DOI	10.3389/fcimb.2019.00182
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Article: Function of KvLQT1 potassium channels in a mouse model of bleomycin-induced acute lung injury.

Aubin Vega, Mélissa / Girault, Alban / Meunier, Émilie / Chebli, Jasmine / Privé, Anik / Robichaud, Annette / Adam, Damien / Brochiero, Emmanuelle

Frontiers in physiology

2024 Volume 15, Page(s) 1345488

Abstract: Acute respiratory distress syndrome (ARDS) is characterized by an exacerbated inflammatory response, severe damage to the alveolar-capillary barrier and a secondary infiltration of protein-rich fluid into the airspaces, ultimately leading to respiratory ... ...

Abstract	Acute respiratory distress syndrome (ARDS) is characterized by an exacerbated inflammatory response, severe damage to the alveolar-capillary barrier and a secondary infiltration of protein-rich fluid into the airspaces, ultimately leading to respiratory failure. Resolution of ARDS depends on the ability of the alveolar epithelium to reabsorb lung fluid through active transepithelial ion transport, to control the inflammatory response, and to restore a cohesive and functional epithelium through effective repair processes. Interestingly, several lines of evidence have demonstrated the important role of potassium (K
Language	English
Publishing date	2024-02-20
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2564217-0
ISSN	1664-042X
ISSN	1664-042X
DOI	10.3389/fphys.2024.1345488
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Article: Impact of KvLQT1 potassium channel modulation on alveolar fluid homeostasis in an animal model of thiourea-induced lung edema.

Aubin Vega, Mélissa / Girault, Alban / Adam, Damien / Chebli, Jasmine / Privé, Anik / Maillé, Émilie / Robichaud, Annette / Brochiero, Emmanuelle

Frontiers in physiology

2023 Volume 13, Page(s) 1069466

Abstract: Alveolar ion and fluid absorption is essential for lung homeostasis in healthy conditions as well as for the resorption of lung edema, a key feature of acute respiratory distress syndrome. Liquid absorption is driven by active transepithelial sodium ... ...

Abstract	Alveolar ion and fluid absorption is essential for lung homeostasis in healthy conditions as well as for the resorption of lung edema, a key feature of acute respiratory distress syndrome. Liquid absorption is driven by active transepithelial sodium transport, through apical ENaC Na
Language	English
Publishing date	2023-01-09
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2564217-0
ISSN	1664-042X
ISSN	1664-042X
DOI	10.3389/fphys.2022.1069466
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Article ; Online: Evaluation of interleukin-1 and interleukin-6 receptor antagonists in a murine model of acute lung injury.

Meunier, Émilie / Aubin Vega, Mélissa / Adam, Damien / Privé, Anik / Mohammad Nezhady, Mohammad Ali / Lahaie, Isabelle / Quiniou, Christiane / Chemtob, Sylvain / Brochiero, Emmanuelle

Experimental physiology

2024

Abstract: The acute exudative phase of acute respiratory distress syndrome (ARDS), a severe form of respiratory failure, is characterized by alveolar damage, pulmonary oedema, and an exacerbated inflammatory response. There is no effective treatment for this ... ...

Abstract	The acute exudative phase of acute respiratory distress syndrome (ARDS), a severe form of respiratory failure, is characterized by alveolar damage, pulmonary oedema, and an exacerbated inflammatory response. There is no effective treatment for this condition, but based on the major contribution of inflammation, anti-inflammatory strategies have been evaluated in animal models and clinical trials, with conflicting results. In COVID-19 ARDS patients, interleukin (IL)-1 and IL-6 receptor antagonists (IL-1Ra and IL-6Ra, kineret and tocilizumab, respectively) have shown some efficacy. Moreover, we have previously developed novel peptides modulating IL-1R and IL-6R activity (rytvela and HSJ633, respectively) while preserving immune vigilance and cytoprotective pathways. We aimed to assess the efficacy of these novel IL-1Ra and IL-6Ra, compared to commercially available drugs (kineret, tocilizumab) during the exudative phase (day 7) of bleomycin-induced acute lung injury (ALI) in mice. Our results first showed that none of the IL-1Ra and IL-6Ra compounds attenuated bleomycin-induced weight loss and venous
Language	English
Publishing date	2024-04-09
Publishing country	England
Document type	Journal Article
ZDB-ID	1016295-1
ISSN	1469-445X ; 0958-0670
ISSN (online)	1469-445X
ISSN	0958-0670
DOI	10.1113/EP091682
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Article ; Online: Intracellular

Malet, Karim / Faure, Emmanuel / Adam, Damien / Donner, Jannik / Liu, Lin / Pilon, Sarah-Jeanne / Fraser, Richard / Jorth, Peter / Newman, Dianne K / Brochiero, Emmanuelle / Rousseau, Simon / Nguyen, Dao

American journal of respiratory and critical care medicine

2024

Abstract: Rationale: Pseudomonas aeruginosa: Objectives: To detect and characterize intracellular : Methods: We sampled the lung explant tissues from CF patients undergoing lung transplantation and non-CF lung donor control. We analyzed lung tissue sections ...

Abstract	Rationale: Pseudomonas aeruginosa Objectives: To detect and characterize intracellular Methods: We sampled the lung explant tissues from CF patients undergoing lung transplantation and non-CF lung donor control. We analyzed lung tissue sections for the presence of intracellular Measurements and main results: P.a. Conclusion: This is the first study describing the presence of intracellular
Language	English
Publishing date	2024-02-07
Publishing country	United States
Document type	Journal Article
ZDB-ID	1180953-x
ISSN	1535-4970 ; 0003-0805 ; 1073-449X
ISSN (online)	1535-4970
ISSN	0003-0805 ; 1073-449X
DOI	10.1164/rccm.202308-1451OC
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Article ; Online: Efficient Transcriptionally Controlled Plasmid Expression System for Investigation of the Stability of mRNA Transcripts in Primary Alveolar Epithelial Cells.

Migneault, Francis / Gagnon, Frédéric / Brochiero, Emmanuelle / Berthiaume, Yves / Dagenais, André

Journal of visualized experiments : JoVE

2020 , Issue 157

Abstract: Studying posttranscriptional regulation is fundamental to understanding the modulation of a given messenger RNA (mRNA) and its impact on cell homeostasis and metabolism. Indeed, fluctuations in transcript expression could modify the translation ... ...

Abstract	Studying posttranscriptional regulation is fundamental to understanding the modulation of a given messenger RNA (mRNA) and its impact on cell homeostasis and metabolism. Indeed, fluctuations in transcript expression could modify the translation efficiency and ultimately the cellular activity of a transcript. Several experimental approaches have been developed to investigate the half-life of mRNA although some of these methods have limitations that prevent the proper study of posttranscriptional modulation. A promoter induction system can express a gene of interest under the control of a synthetic tetracycline-regulated promoter. This method allows the half-life estimation of a given mRNA under any experimental condition without disturbing cell homeostasis. One major drawback of this method is the necessity to transfect cells, which limits the use of this technique in isolated primary cells that are highly resistant to conventional transfection techniques. Alveolar epithelial cells in primary culture have been used extensively to study the cellular and molecular biology of the alveolar epithelium. The unique characteristics and phenotype of primary alveolar cells make it essential to study the posttranscriptional modulations of genes of interest in these cells. Therefore, our aim was to develop a novel tool to investigate the posttranscriptional modulations of mRNAs of interest in alveolar epithelial cells in primary culture. We designed a fast and efficient transient transfection protocol to insert a transcriptionally controlled plasmid expression system into primary alveolar epithelial cells. This cloning strategy, using a viral epitope to tag the construct, allows for the easy discrimination of construct expression from that of endogenous mRNAs. Using a modified ΔΔ quantification cycle (Cq) method, the expression of the transcript can then be quantified at different time intervals to measure its half-life. Our data demonstrate the efficiency of this novel approach in studying posttranscriptional regulation in various pathophysiological conditions in primary alveolar epithelial cells.
MeSH term(s)	3' Untranslated Regions/genetics ; Alveolar Epithelial Cells/drug effects ; Alveolar Epithelial Cells/metabolism ; Animals ; Cells, Cultured ; DNA Primers/metabolism ; Dactinomycin/pharmacology ; Doxycycline/pharmacology ; Electroporation ; Epithelial Sodium Channels/genetics ; Epithelial Sodium Channels/metabolism ; Gene Expression Regulation/drug effects ; Inflammation/pathology ; Kinetics ; Plasmids/genetics ; RNA Stability/drug effects ; RNA Stability/genetics ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; RNA-Binding Proteins/metabolism ; Rats ; Transcription, Genetic/drug effects ; Transfection
Chemical Substances	3' Untranslated Regions ; DNA Primers ; Epithelial Sodium Channels ; RNA, Messenger ; RNA-Binding Proteins ; Dactinomycin (1CC1JFE158) ; Doxycycline (N12000U13O)
Language	English
Publishing date	2020-03-06
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Video-Audio Media
ZDB-ID	2259946-0
ISSN	1940-087X ; 1940-087X
ISSN (online)	1940-087X
ISSN	1940-087X
DOI	10.3791/60654
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Article ; Online: Impact of ENaC downregulation in transgenic mice on the outcomes of acute lung injury induced by bleomycin.

Aubin Vega, Mélissa / Chupin, Cécile / Massé, Chantal / Dagenais, André / Berthiaume, Yves / Brochiero, Emmanuelle

Experimental physiology

2021 Volume 106, Issue 4, Page(s) 1110–1119

Abstract: New findings: What is the central question of this study? How does the downregulation of ENaC, the major driving force for alveolar fluid clearance, impact acute lung injury outcomes induced by bleomycin, featuring alveolar damage, as observed during ... ...

Abstract	New findings: What is the central question of this study? How does the downregulation of ENaC, the major driving force for alveolar fluid clearance, impact acute lung injury outcomes induced by bleomycin, featuring alveolar damage, as observed during ARDS exudative phase? What is the main finding and its importance? ENaC downregulation in αENaC(-/-)Tg+ mice did not elicit a substantial worsening impact on the main bleomycin outcomes. In ARDS patients, both ENaC alteration and alveolar damage are observed. Thus, novel therapeutic avenues, favouring alveolar integrity restauration, in addition to lung oedema resolution capacity, mainly driven by ENaC, would be essential. Abstract: The exudative phase of acute respiratory distress syndrome (ARDS) is characterized by extended alveolar damage, resulting in accumulation of protein-rich inflammatory oedematous fluid in the alveolar space. Na
MeSH term(s)	Acute Lung Injury/chemically induced ; Animals ; Bleomycin ; Down-Regulation ; Epithelial Sodium Channels/genetics ; Epithelial Sodium Channels/metabolism ; Humans ; Lung/metabolism ; Mice ; Mice, Transgenic
Chemical Substances	Epithelial Sodium Channels ; Bleomycin (11056-06-7)
Language	English
Publishing date	2021-02-15
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1016295-1
ISSN	1469-445X ; 0958-0670
ISSN (online)	1469-445X
ISSN	0958-0670
DOI	10.1113/EP089060
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Article: Efficient transcriptionally controlled plasmid expression system for investigation of the stability of mrna transcripts in primary alveolar epithelial cells

Migneault, Francis / Gagnon, Frédéric / Brochiero, Emmanuelle / Berthiaume, Yves / Dagenais, André

Journal of visualized experiments. 2020 Mar. 06, , no. 157

2020

Abstract	Studying posttranscriptional regulation is fundamental to understanding the modulation of a given messenger RNA (mRNA) and its impact on cell homeostasis and metabolism. Indeed, fluctuations in transcript expression could modify the translation efficiency and ultimately the cellular activity of a transcript. Several experimental approaches have been developed to investigate the half-life of mRNA although some of these methods have limitations that prevent the proper study of posttranscriptional modulation. A promoter induction system can express a gene of interest under the control of a synthetic tetracycline-regulated promoter. This method allows the half-life estimation of a given mRNA under any experimental condition without disturbing cell homeostasis. One major drawback of this method is the necessity to transfect cells, which limits the use of this technique in isolated primary cells that are highly resistant to conventional transfection techniques. Alveolar epithelial cells in primary culture have been used extensively to study the cellular and molecular biology of the alveolar epithelium. The unique characteristics and phenotype of primary alveolar cells make it essential to study the posttranscriptional modulations of genes of interest in these cells. Therefore, our aim was to develop a novel tool to investigate the posttranscriptional modulations of mRNAs of interest in alveolar epithelial cells in primary culture. We designed a fast and efficient transient transfection protocol to insert a transcriptionally controlled plasmid expression system into primary alveolar epithelial cells. This cloning strategy, using a viral epitope to tag the construct, allows for the easy discrimination of construct expression from that of endogenous mRNAs. Using a modified ΔΔ quantification cycle (Cq) method, the expression of the transcript can then be quantified at different time intervals to measure its half-life. Our data demonstrate the efficiency of this novel approach in studying posttranscriptional regulation in various pathophysiological conditions in primary alveolar epithelial cells.
Keywords	epithelial cells ; epithelium ; epitopes ; genes ; half life ; homeostasis ; messenger RNA ; metabolism ; molecular biology ; phenotype ; plasmids ; transcription (genetics) ; transfection
Language	English
Dates of publication	2020-0306
Size	p. e60654.
Publishing place	Journal of Visualized Experiments
Document type	Article
ZDB-ID	2259946-0
ISSN	1940-087X
ISSN	1940-087X
DOI	10.3791/60654
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Utilization of Transcriptomic Profiling to Identify Molecular Markers Predicting Successful Recovery Following Endoscopic Sinus Surgery for Chronic Rhinosinusitis.

Renteria, Axel E / Maniakas, Anastasios / Pelletier, Audrey / Filali-Mouhim, Ali / Brochiero, Emmanuelle / Valera, Fabiana C P / Adam, Damien / Mfuna, Leandra Endam / Desrosiers, Martin

Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery

2023 Volume 169, Issue 6, Page(s) 1662–1673

Abstract: Objectives: Successful recovery from chronic rhinosinusitis (CRS) following endoscopic sinus surgery (ESS) can be characterized by minimal presence of symptoms and absence of disease on endoscopy. However, molecular markers of surgical success remain to ...

Abstract	Objectives: Successful recovery from chronic rhinosinusitis (CRS) following endoscopic sinus surgery (ESS) can be characterized by minimal presence of symptoms and absence of disease on endoscopy. However, molecular markers of surgical success remain to be characterized. These could allow for better tailoring of perioperative therapy. This study aims to identify novel molecular markers associated with surgery responsive patient. Study design: Prospective cohort study. Setting: Single academic hospital center. Method: One hundred eighteen consecutive patients with CRS at high risk of recurrence after surgery were followed prospectively following ESS in an academic medical center. Symptomatic and endoscopic outcomes were assessed at 4 months, with success rigorously defined subjectively as minimal or no symptoms (no symptom greater than 1 on an ordinal scale of 0-3) and objectively by the absence of nasal polyposis on sinus cavity endoscopy and Lund-Kennedy endoscopic edema score no greater than 1. Samples were obtained at the time of surgery and at 4-month postoperatively. Changes associated with surgery were determined by gene expression profiling using Affymetrix's Clariom S Human HT arrays. Results: Successful ESS was characterized by a mild upregulation in Type 1 inflammation, upregulation of cell cycle progression, and epithelial barrier and proliferation-associated genes and pathways. ESS failure was associated to very high levels of Type 1 inflammation along with downregulation of epithelial barrier function and regeneration genes and pathways. Conclusion: Successful recovery from ESS involves restoration of epithelial function and regulated activation of Type 1 inflammation. Excessively elevated Type 1 inflammation is associated with epithelial barrier dysfunction.
MeSH term(s)	Humans ; Prospective Studies ; Transcriptome ; Rhinitis/genetics ; Rhinitis/surgery ; Rhinitis/complications ; Sinusitis/genetics ; Sinusitis/surgery ; Sinusitis/complications ; Inflammation/complications ; Nasal Polyps/genetics ; Nasal Polyps/surgery ; Nasal Polyps/complications ; Biomarkers ; Endoscopy ; Chronic Disease ; Gene Expression Profiling ; Treatment Outcome
Chemical Substances	Biomarkers
Language	English
Publishing date	2023-08-25
Publishing country	England
Document type	Journal Article
ZDB-ID	392085-9
ISSN	1097-6817 ; 0161-6439 ; 0194-5998
ISSN (online)	1097-6817
ISSN	0161-6439 ; 0194-5998
DOI	10.1002/ohn.482
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Article ; Online: Association of low-density neutrophils with lung function and disease progression in adult cystic fibrosis.

Murru, Andréa / Vadeboncoeur, Nathalie / Therrien, Andrée-Anne / Coderre, Lise / Vaillancourt, Myriam / Labrecque, Marie-Michèle / Berthiaume, Yves / Bouvet, Guillaume / Adam, Damien / Brochiero, Emmanuelle / Lesage, Sylvie / Flamand, Nicolas / Bilodeau, Lara / Fernandes, Maria J

Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society

2023 Volume 22, Issue 6, Page(s) 1080–1084

Abstract: Background: Cystic fibrosis (CF) neutrophils fail to eradicate infection despite their massive recruitment into the lung. While studies mostly focus on pathogen clearance by normal density neutrophils in CF, the contribution of low-density neutrophil ( ... ...

Abstract	Background: Cystic fibrosis (CF) neutrophils fail to eradicate infection despite their massive recruitment into the lung. While studies mostly focus on pathogen clearance by normal density neutrophils in CF, the contribution of low-density neutrophil (LDNs) subpopulations to disease pathogenesis remains unclear. Methods: LDNs were isolated from whole blood donations of clinically stable adult CF patients and from healthy donors. LDN proportion and immunophenotype was assessed by flow cytometry. Associations of LDNs with clinical parameters were determined. Results: LDN proportion was increased in CF patients' circulation compared with healthy donors. LDNs are a heterogeneous population of both mature and immature cells in CF and in healthy individuals. Moreover, a higher proportion of mature LDN correlates with a gradual decline in lung function and repeated pulmonary exacerbations in CF patients. Conclusions: Collectively, our observations suggest that low-density neutrophils are linked to CF pathogenesis and underscore the potential clinical relevance of neutrophil subpopulations in CF.
MeSH term(s)	Humans ; Adult ; Cystic Fibrosis ; Neutrophils/pathology ; Lung ; Disease Progression
Language	English
Publishing date	2023-03-25
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	2084724-5
ISSN	1873-5010 ; 1569-1993
ISSN (online)	1873-5010
ISSN	1569-1993
DOI	10.1016/j.jcf.2023.03.009
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