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Artikel ; Online: Sources of Variance in Human Tear Proteomic Samples: Statistical Evaluation, Quality Control, Normalization, and Biological Insight.

Bruszel, Bella / Tóth-Molnár, Edit / Janáky, Tamás / Szabó, Zoltán

International journal of molecular sciences

2024 Band 25, Heft 3

Abstract: Human tear fluid contains numerous compounds, which are present in highly variable amounts owing to the dynamic and multipurpose functions of tears. A better understanding of the level and sources of variance is essential for determining the functions of ...

Abstract	Human tear fluid contains numerous compounds, which are present in highly variable amounts owing to the dynamic and multipurpose functions of tears. A better understanding of the level and sources of variance is essential for determining the functions of the different tear components and the limitations of tear samples as a potential biomarker source. In this study, a quantitative proteomic method was used to analyze variations in the tear protein profiles of healthy volunteers. High day-to-day and inter-eye personal variances were observed in the tear volumes, protein content, and composition of the tear samples. Several normalization and outlier exclusion approaches were evaluated to decrease variances. Despite the intrapersonal variances, statistically significant differences and cluster analysis revealed that proteome profile and immunoglobulin composition of tear fluid present personal characteristics. Using correlation analysis, we could identify several correlating protein clusters, mainly related to the source of the proteins. Our study is the first attempt to achieve more insight into the biochemical background of human tears by statistical evaluation of the experimentally observed dynamic behavior of the tear proteome. As a pilot study for determination of personal protein profiles of the tear fluids of individual patients, it contributes to the application of this noninvasively collectible body fluid in personal medicine.
Mesh-Begriff(e)	Humans ; Proteome/metabolism ; Proteomics/methods ; Pilot Projects ; Tears/metabolism ; Eye Proteins/metabolism ; Quality Control
Chemische Substanzen	Proteome ; Eye Proteins
Sprache	Englisch
Erscheinungsdatum	2024-01-26
Erscheinungsland	Switzerland
Dokumenttyp	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms25031559
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel ; Online: Diet-Induced Hypercholesterolemia Leads to Cardiac Dysfunction and Alterations in the Myocardial Proteome.

Szabó, Márton Richárd / Pipicz, Márton / Sárközy, Márta / Bruszel, Bella / Szabó, Zoltán / Csont, Tamás

International journal of molecular sciences

2022 Band 23, Heft 13

Abstract: Elevated blood cholesterol is a major risk factor for coronary heart disease. Moreover, direct effects on the myocardium also contribute to the adverse effects of hypercholesterolemia. Here, we investigated the effect of hypercholesterolemia on the ... ...

Abstract	Elevated blood cholesterol is a major risk factor for coronary heart disease. Moreover, direct effects on the myocardium also contribute to the adverse effects of hypercholesterolemia. Here, we investigated the effect of hypercholesterolemia on the cardiac proteome. Male Wistar rats were fed with a laboratory rodent chow supplemented with 2% cholesterol for 8 weeks to induce hypercholesterolemia. The protein expression data obtained from the proteomic characterization of left ventricular samples from normo- and hypercholesterolemic animals were subjected to gene ontology (GO) and protein interaction analyses. Elevated circulating cholesterol levels were accompanied by diastolic dysfunction in cholesterol-fed rats. The proteomic characterization of left ventricular samples revealed altered expression of 45 proteins due to hypercholesterolemia. Based on the Gene Ontology analysis, hypercholesterolemia was associated with disturbed expression of cytoskeletal and contractile proteins. Beta-actin was downregulated in the hypercholesterolemic myocardium, and established a prominent hub of the protein interaction network. Analysis of the unfiltered dataset revealed concordant downregulated expression patterns in proteins associated with the arrangement of the contractile system (e.g., cardiac-specific troponins and myosin complex), and in subunits of the mitochondrial respiratory chain. We conclude that the observed changes in the cardiac proteome may contribute to the development of diastolic dysfunction in hypercholesterolemia.
Mesh-Begriff(e)	Animals ; Cholesterol/metabolism ; Diet ; Heart Diseases/metabolism ; Hypercholesterolemia/metabolism ; Male ; Myocardium/metabolism ; Proteome/metabolism ; Proteomics ; Rats ; Rats, Wistar
Chemische Substanzen	Proteome ; Cholesterol (97C5T2UQ7J)
Sprache	Englisch
Erscheinungsdatum	2022-07-02
Erscheinungsland	Switzerland
Dokumenttyp	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms23137387
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel ; Online: Synthesis and Antiproliferative Activity of Steroidal Diaryl Ethers.

Kovács, Édua / Ali, Hazhmat / Minorics, Renáta / Traj, Péter / Resch, Vivien / Paragi, Gábor / Bruszel, Bella / Zupkó, István / Mernyák, Erzsébet

Molecules (Basel, Switzerland)

2023 Band 28, Heft 3

Abstract: Novel 13α-estrone derivatives have been synthesized via direct arylation of the phenolic hydroxy function. Chan-Lam couplings of arylboronic acids with 13α-estrone as a nucleophilic partner were carried out under copper catalysis. The antiproliferative ... ...

Abstract	Novel 13α-estrone derivatives have been synthesized via direct arylation of the phenolic hydroxy function. Chan-Lam couplings of arylboronic acids with 13α-estrone as a nucleophilic partner were carried out under copper catalysis. The antiproliferative activities of the newly synthesized diaryl ethers against a panel of human cancer cell lines (A2780, MCF-7, MDA-MB 231, HeLa, SiHa) were investigated by means of MTT assays. The quinoline derivative displayed substantial antiproliferative activity against MCF-7 and HeLa cell lines with low micromolar IC
Mesh-Begriff(e)	Humans ; Female ; HeLa Cells ; Cell Line, Tumor ; Antineoplastic Agents/chemistry ; Estrone/chemistry ; Tubulin/metabolism ; Ethers/pharmacology ; Ovarian Neoplasms ; Cell Proliferation ; Drug Screening Assays, Antitumor ; Structure-Activity Relationship ; Molecular Structure
Chemische Substanzen	Antineoplastic Agents ; Estrone (2DI9HA706A) ; Tubulin ; Ethers
Sprache	Englisch
Erscheinungsdatum	2023-01-25
Erscheinungsland	Switzerland
Dokumenttyp	Journal Article
ZDB-ID	1413402-0
ISSN	1420-3049 ; 1431-5165 ; 1420-3049
ISSN (online)	1420-3049
ISSN	1431-5165 ; 1420-3049
DOI	10.3390/molecules28031196
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Zs.MO 81: Hefte anzeigen

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Artikel ; Online: Indoleamine 2,3-Dioxygenase Cannot Inhibit

Virok, Dezső P / Tömösi, Ferenc / Keller-Pintér, Anikó / Szabó, Kitti / Bogdanov, Anita / Poliska, Szilárd / Rázga, Zsolt / Bruszel, Bella / Cseh, Zsuzsanna / Kókai, Dávid / Paróczai, Dóra / Endrész, Valéria / Janáky, Tamás / Burián, Katalin

Frontiers in immunology

2021 Band 12, Seite(n) 717311

Abstract: Aims: Neutrophil granulocytes are the major cells involved in : Methods: Global gene expression screen was used to evaluate the effect of : Results: Our results indicate that the : Conclusions: IDO activity was not able to inhibit chlamydial ... ...

Abstract	Aims: Neutrophil granulocytes are the major cells involved in Methods: Global gene expression screen was used to evaluate the effect of Results: Our results indicate that the Conclusions: IDO activity was not able to inhibit chlamydial growth in human neutrophils. Whether the IDO activity was not high enough for inhibition or other chlamydial growth-promoting host mechanisms were induced in the infected and interferon-treated neutrophils needs to be further investigated.
Mesh-Begriff(e)	Chlamydia Infections/enzymology ; Chlamydia Infections/immunology ; Chlamydia Infections/microbiology ; Chlamydia trachomatis/growth & development ; Chlamydia trachomatis/immunology ; Chlamydia trachomatis/metabolism ; HL-60 Cells ; HeLa Cells ; Humans ; Indoleamine-Pyrrole 2,3,-Dioxygenase/genetics ; Indoleamine-Pyrrole 2,3,-Dioxygenase/metabolism ; Interferon-gamma/pharmacology ; Metabolome ; Neutrophils/drug effects ; Neutrophils/enzymology ; Transcriptome ; Tryptophan/metabolism
Chemische Substanzen	IDO1 protein, human ; IFNG protein, human ; Indoleamine-Pyrrole 2,3,-Dioxygenase ; Interferon-gamma (82115-62-6) ; Tryptophan (8DUH1N11BX)
Sprache	Englisch
Erscheinungsdatum	2021-11-08
Erscheinungsland	Switzerland
Dokumenttyp	Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2606827-8
ISSN	1664-3224 ; 1664-3224
ISSN (online)	1664-3224
ISSN	1664-3224
DOI	10.3389/fimmu.2021.717311
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel ; Online: Microwave-assisted Phospha-Michael addition reactions in the 13α-oestrone series and

Mernyák, Erzsébet / Bartha, Sándor / Kóczán, Lili / Jójárt, Rebeka / Resch, Vivien / Paragi, Gábor / Vágvölgyi, Máté / Hunyadi, Attila / Bruszel, Bella / Zupkó, István / Minorics, Renáta

Journal of enzyme inhibition and medicinal chemistry

2021 Band 36, Heft 1, Seite(n) 1931–1937

Abstract: Microwave-assisted phospha-Michael addition reactions were carried out in the 13α-oestrone series. The exocyclic 16-methylene-17-ketones as α,β-unsaturated ketones were reacted with secondary phosphine oxides as nucleophilic partners. The addition ... ...

Abstract	Microwave-assisted phospha-Michael addition reactions were carried out in the 13α-oestrone series. The exocyclic 16-methylene-17-ketones as α,β-unsaturated ketones were reacted with secondary phosphine oxides as nucleophilic partners. The addition reactions furnished the two tertiary phosphine oxide diastereomers in high yields. The main product was the 16α-isomer. The antiproliferative activities of the newly synthesised organophosphorus compounds against a panel of nine human cancer cell lines were investigated by means of MTT assays. The most potent compound, the diphenylphosphine oxide derivative in the 3-
Sprache	Englisch
Erscheinungsdatum	2021-08-23
Erscheinungsland	England
Dokumenttyp	Journal Article
ZDB-ID	2082578-X
ISSN	1475-6374 ; 1475-6366
ISSN (online)	1475-6374
ISSN	1475-6366
DOI	10.1080/14756366.2021.1963241
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Zs.A 3004: Hefte anzeigen

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Artikel ; Online: Small Extracellular Vesicles Isolated from Serum May Serve as Signal-Enhancers for the Monitoring of CNS Tumors.

Dobra, Gabriella / Bukva, Matyas / Szabo, Zoltan / Bruszel, Bella / Harmati, Maria / Gyukity-Sebestyen, Edina / Jenei, Adrienn / Szucs, Monika / Horvath, Peter / Biro, Tamas / Klekner, Almos / Buzas, Krisztina

International journal of molecular sciences

2020 Band 21, Heft 15

Abstract: Liquid biopsy-based methods to test biomarkers (e.g., serum proteins and extracellular vesicles) may help to monitor brain tumors. In this proteomics-based study, we aimed to identify a characteristic protein fingerprint associated with central nervous ... ...

Abstract	Liquid biopsy-based methods to test biomarkers (e.g., serum proteins and extracellular vesicles) may help to monitor brain tumors. In this proteomics-based study, we aimed to identify a characteristic protein fingerprint associated with central nervous system (CNS) tumors. Overall, 96 human serum samples were obtained from four patient groups, namely glioblastoma multiforme (GBM), non-small-cell lung cancer brain metastasis (BM), meningioma (M) and lumbar disc hernia patients (CTRL). After the isolation and characterization of small extracellular vesicles (sEVs) by nanoparticle tracking analysis (NTA) and atomic force microscopy (AFM), liquid chromatography -mass spectrometry (LC-MS) was performed on two different sample types (whole serum and serum sEVs). Statistical analyses (ratio, Cohen's d, receiver operating characteristic; ROC) were carried out to compare patient groups. To recognize differences between the two sample types, pairwise comparisons (Welch's test) and ingenuity pathway analysis (IPA) were performed. According to our knowledge, this is the first study that compares the proteome of whole serum and serum-derived sEVs. From the 311 proteins identified, 10 whole serum proteins and 17 sEV proteins showed the highest intergroup differences. Sixty-five proteins were significantly enriched in sEV samples, while 129 proteins were significantly depleted compared to whole serum. Based on principal component analysis (PCA) analyses, sEVs are more suitable to discriminate between the patient groups. Our results support that sEVs have greater potential to monitor CNS tumors, than whole serum.
Mesh-Begriff(e)	Adult ; Aged ; Aged, 80 and over ; Biomarkers, Tumor/blood ; Carcinoma, Non-Small-Cell Lung/blood ; Extracellular Vesicles/metabolism ; Female ; Humans ; Lung Neoplasms/blood ; Male ; Meningeal Neoplasms/blood ; Meningeal Neoplasms/secondary ; Middle Aged ; Neoplasm Proteins/blood
Chemische Substanzen	Biomarkers, Tumor ; Neoplasm Proteins
Sprache	Englisch
Erscheinungsdatum	2020-07-28
Erscheinungsland	Switzerland
Dokumenttyp	Clinical Trial ; Journal Article ; Multicenter Study
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms21155359
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel ; Online: Prediabetes Induced by Fructose-Enriched Diet Influences Cardiac Lipidome and Proteome and Leads to Deterioration of Cardiac Function prior to the Development of Excessive Oxidative Stress and Cell Damage.

Szűcs, Gergő / Sója, Andrea / Péter, Mária / Sárközy, Márta / Bruszel, Bella / Siska, Andrea / Földesi, Imre / Szabó, Zoltán / Janáky, Tamás / Vígh, László / Balogh, Gábor / Csont, Tamás

Oxidative medicine and cellular longevity

2019 Band 2019, Seite(n) 3218275

Abstract: Prediabetes is a condition affecting more than 35% of the population. In some forms, excessive carbohydrate intake (primarily refined sugar) plays a prominent role. Prediabetes is a symptomless, mostly unrecognized disease which increases cardiovascular ... ...

Abstract	Prediabetes is a condition affecting more than 35% of the population. In some forms, excessive carbohydrate intake (primarily refined sugar) plays a prominent role. Prediabetes is a symptomless, mostly unrecognized disease which increases cardiovascular risk. In our work, we examined the effect of a fructose-enriched diet on cardiac function and lipidome as well as proteome of cardiac muscle. Male Wistar rats were divided into two groups. The control group received a normal diet while the fructose-fed group received 60% fructose-supplemented chow for 24 weeks. Fasting blood glucose measurement and oral glucose tolerance test (OGTT) showed slightly but significantly elevated values due to fructose feeding indicating development of a prediabetic condition. Both echocardiography and isolated working heart perfusion performed at the end of the feeding protocol demonstrated diastolic cardiac dysfunction in the fructose-fed group. Mass spectrometry-based, high-performance lipidomic and proteomic analyses were executed from cardiac tissue. The lipidomic analysis revealed complex rearrangement of the whole lipidome with special emphasis on defects in cardiolipin remodeling. The proteomic analysis showed significant changes in 75 cardiac proteins due to fructose feeding including mitochondria-, apoptosis-, and oxidative stress-related proteins. Nevertheless, just very weak or no signs of apoptosis induction and oxidative stress were detected in the hearts of fructose-fed rats. Our results suggest that fructose feeding induces marked alterations in the cardiac lipidome, especially in cardiolipin remodeling, which leads to mitochondrial dysfunction and impaired cardiac function. However, at the same time, several adaptive responses are induced at the proteome level in order to maintain a homeostatic balance. These findings demonstrate that even very early stages of prediabetes can impair cardiac function and can result in significant changes in the lipidome and proteome of the heart prior to the development of excessive oxidative stress and cell damage.
Mesh-Begriff(e)	Animals ; Cardiovascular Diseases/genetics ; Disease Models, Animal ; Echocardiography/methods ; Fructose/adverse effects ; Humans ; Lipidomics/methods ; Male ; Oxidative Stress ; Prediabetic State/complications ; Proteomics/methods ; Rats ; Rats, Wistar
Chemische Substanzen	Fructose (30237-26-4)
Sprache	Englisch
Erscheinungsdatum	2019-12-09
Erscheinungsland	United States
Dokumenttyp	Journal Article
ISSN	1942-0994
ISSN (online)	1942-0994
DOI	10.1155/2019/3218275
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel ; Online: Sources of Variance in Human Tear Proteomic Samples: Statistical Evaluation, Quality Control, Normalization, and Biological Insight.

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Artikel ; Online: Diet-Induced Hypercholesterolemia Leads to Cardiac Dysfunction and Alterations in the Myocardial Proteome.

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Artikel ; Online: Synthesis and Antiproliferative Activity of Steroidal Diaryl Ethers.

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Artikel ; Online: Indoleamine 2,3-Dioxygenase Cannot Inhibit

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Artikel ; Online: Microwave-assisted Phospha-Michael addition reactions in the 13α-oestrone series and

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Artikel ; Online: Small Extracellular Vesicles Isolated from Serum May Serve as Signal-Enhancers for the Monitoring of CNS Tumors.

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Artikel ; Online: Prediabetes Induced by Fructose-Enriched Diet Influences Cardiac Lipidome and Proteome and Leads to Deterioration of Cardiac Function prior to the Development of Excessive Oxidative Stress and Cell Damage.

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