LIVIVO - Search results -

Search results

Result 1 - 10 of total 12

Search options

Article ; Online: Cefazolin as a predictor of urinary cephalosporin activity in indicated Enterobacterales.

Bryson, Alexandra L / Bhalodi, Amira / Liesman, Rachael M / Mathers, Amy J

2024 Volume 62, Issue 4, Page(s) e0078821

Abstract: Traditionally, cephalothin susceptibility results were used to predict the susceptibility of additional cephalosporins; however, in 2013-2014, the Clinical and Laboratory Standards Institute (CLSI) revisited this practice and determined that cefazolin is ...

Abstract	Traditionally, cephalothin susceptibility results were used to predict the susceptibility of additional cephalosporins; however, in 2013-2014, the Clinical and Laboratory Standards Institute (CLSI) revisited this practice and determined that cefazolin is a more accurate proxy than cephalothin for uncomplicated urinary tract infections (uUTIs). Therefore, a cefazolin surrogacy breakpoint was established to predict the susceptibility of seven oral cephalosporins for
MeSH term(s)	Humans ; Cefazolin/pharmacology ; Cefazolin/therapeutic use ; Cephalosporins/pharmacology ; Cephalothin ; Anti-Bacterial Agents/pharmacology ; Anti-Bacterial Agents/therapeutic use ; Microbial Sensitivity Tests ; Urinary Tract Infections/drug therapy ; Urinary Tract Infections/microbiology ; Escherichia coli ; Monobactams
Chemical Substances	Cefazolin (IHS69L0Y4T) ; Cephalosporins ; Cephalothin (R72LW146E6) ; Anti-Bacterial Agents ; Monobactams
Language	English
Publishing date	2024-03-08
Publishing country	United States
Document type	Journal Article ; Review
ZDB-ID	390499-4
ISSN	1098-660X ; 0095-1137
ISSN (online)	1098-660X
ISSN	0095-1137
DOI	10.1128/jcm.00788-21
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 1188: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Emergomyces pasteurianus in Man Returning to the United States from Liberia and Review of the Literature.

Pierce, Jacob / Sayeed, Sadia / Doern, Christopher D / Bryson, Alexandra L

Emerging infectious diseases

2023 Volume 29, Issue 3, Page(s) 635–639

Abstract: A 65-year-old man with HIV sought treatment for fever, weight loss, and productive cough after returning to the United States from Liberia. Fungal cultures grew Emergomyces pasteurianus, and the patient's health improved after beginning voriconazole. We ... ...

Abstract	A 65-year-old man with HIV sought treatment for fever, weight loss, and productive cough after returning to the United States from Liberia. Fungal cultures grew Emergomyces pasteurianus, and the patient's health improved after beginning voriconazole. We describe the clinical case and review the literature, treatment, and susceptibilities for E. pasteurianus.
MeSH term(s)	Humans ; United States ; Aged ; Mycoses/microbiology ; Liberia ; Onygenales ; Voriconazole
Chemical Substances	Voriconazole (JFU09I87TR)
Language	English
Publishing date	2023-02-16
Publishing country	United States
Document type	Review ; Case Reports ; Journal Article
ZDB-ID	1380686-5
ISSN	1080-6059 ; 1080-6040
ISSN (online)	1080-6059
ISSN	1080-6040
DOI	10.3201/eid2903.221683
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 4778: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Matrix-Assisted Laser Desorption/Ionization Time-of-Flight: The Revolution in Progress.

Bryson, Alexandra L / Hill, Emily M / Doern, Christopher D

Clinics in laboratory medicine

2019 Volume 39, Issue 3, Page(s) 391–404

Abstract: This article summarizes recent advances in the application of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) to new areas of infectious diseases diagnostics. We discuss progress toward routine identification ... ...

Abstract	This article summarizes recent advances in the application of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) to new areas of infectious diseases diagnostics. We discuss progress toward routine identification of mycobacteria and filamentous fungi and direct identification of pathogens from clinical specimens. Of greatest interest is the use of MALDI-TOF MS for identifying organisms from positive blood cultures and from clinical specimens such as urine. Last, We highlight some exciting new possibilities for MALDI-TOF MS phenotypic susceptibility testing for bacteria and yeast.
MeSH term(s)	Bacteria/isolation & purification ; Humans ; Microbiological Techniques/methods ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Language	English
Publishing date	2019-07-02
Publishing country	United States
Document type	Journal Article ; Review
ZDB-ID	604580-7
ISSN	1557-9832 ; 0272-2712
ISSN (online)	1557-9832
ISSN	0272-2712
DOI	10.1016/j.cll.2019.05.010
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 1695: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

Article ; Online: Navigating Clinical Utilization of Direct-from-Specimen Metagenomic Pathogen Detection: Clinical Applications, Limitations, and Testing Recommendations.

Filkins, Laura M / Bryson, Alexandra L / Miller, Steve A / Mitchell, Stephanie L

Clinical chemistry

2020 Volume 66, Issue 11, Page(s) 1381–1395

Abstract: Background: Metagenomic next generation sequencing (mNGS) is becoming increasingly available for pathogen detection directly from clinical specimens. These tests use target-independent, shotgun sequencing to detect potentially unlimited organisms. The ... ...

Abstract	Background: Metagenomic next generation sequencing (mNGS) is becoming increasingly available for pathogen detection directly from clinical specimens. These tests use target-independent, shotgun sequencing to detect potentially unlimited organisms. The promise of this methodology to aid infection diagnosis is demonstrated through early case reports and clinical studies. However, the optimal role of mNGS in clinical microbiology remains uncertain. Content: We reviewed studies reporting clinical use of mNGS for pathogen detection from various specimen types, including cerebrospinal fluid, plasma, lower respiratory specimens, and others. Published clinical study data were critically evaluated and summarized to identify promising clinical indications for mNGS-based testing, to assess the clinical impact of mNGS for each indication, and to recognize test limitations. Based on these clinical studies, early testing recommendations are made to guide clinical utilization of mNGS for pathogen detection. Finally, current barriers to routine clinical laboratory implementation of mNGS tests are highlighted. Summary: The promise of direct-from-specimen mNGS to enable challenging infection diagnoses has been demonstrated through early clinical studies of patients with meningitis or encephalitis, invasive fungal infections, community acquired pneumonia, and other clinical indications. However, the proportion of patient cases with positive clinical impact due to mNGS testing is low in published studies and the cost of testing is high, emphasizing the importance of improving our understanding of 'when to test' and for which patients mNGS testing is appropriate.
MeSH term(s)	Alveolata/genetics ; Bacteria/genetics ; Bacterial Infections/diagnosis ; Body Fluids/microbiology ; Body Fluids/parasitology ; Fungi/genetics ; High-Throughput Nucleotide Sequencing/standards ; Humans ; Metagenomics/standards ; Mycoses/diagnosis ; Protozoan Infections/diagnosis
Language	English
Publishing date	2020-11-02
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	80102-1
ISSN	1530-8561 ; 0009-9147
ISSN (online)	1530-8561
ISSN	0009-9147
DOI	10.1093/clinchem/hvaa183
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Ud II Zs.171: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: 28-Year-Old Man With an Organism Extracted From the Eyelid.

Pierce, Jacob / Dalton, Justin / Stevens, Michael P / Godwin, Melissa / Hill, Aaron / Doern, Christopher D / Bryson, Alexandra L

Clinical infectious diseases : an official publication of the Infectious Diseases Society of America

2021 Volume 72, Issue 11, Page(s) 2049–2051

MeSH term(s)	Eyelids ; Humans ; Male
Language	English
Publishing date	2021-05-11
Publishing country	United States
Document type	Case Reports ; Journal Article
ZDB-ID	1099781-7
ISSN	1537-6591 ; 1058-4838
ISSN (online)	1537-6591
ISSN	1058-4838
DOI	10.1093/cid/ciaa1841
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 1505: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MO 480: Show issues

Order via subito

Details ▾

Article ; Online: Diffuse Skin Nodules in an Oyster Farmer: Disseminated Mycobacterium marinum.

Sann, Khine Z / Vissichelli, Nicole C / Bryson, Alexandra L / Doern, Christopher / Mochel, Mark C / Bearman, Gonzalo / Sastry, Sangeeta

The American journal of medicine

2020 Volume 134, Issue 1, Page(s) e57–e59

MeSH term(s)	Aged ; Agriculture/methods ; Animals ; Exanthema/etiology ; Humans ; Male ; Mycobacterium Infections, Nontuberculous ; Mycobacterium marinum/pathogenicity ; Ostreidae ; Spondylitis, Ankylosing/complications ; Spondylitis, Ankylosing/diagnostic imaging
Language	English
Publishing date	2020-07-22
Publishing country	United States
Document type	Case Reports
ZDB-ID	80015-6
ISSN	1555-7162 ; 1873-2178 ; 0002-9343 ; 1548-2766
ISSN (online)	1555-7162 ; 1873-2178
ISSN	0002-9343 ; 1548-2766
DOI	10.1016/j.amjmed.2020.06.024
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Ua VI Zs.289: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Syndromic Panel-Based Testing in Clinical Microbiology.

Ramanan, Poornima / Bryson, Alexandra L / Binnicker, Matthew J / Pritt, Bobbi S / Patel, Robin

Clinical microbiology reviews

2017 Volume 31, Issue 1

Abstract: The recent development of commercial panel-based molecular diagnostics for the rapid detection of pathogens in positive blood culture bottles, respiratory specimens, stool, and cerebrospinal fluid has resulted in a paradigm shift in clinical microbiology ...

Abstract	The recent development of commercial panel-based molecular diagnostics for the rapid detection of pathogens in positive blood culture bottles, respiratory specimens, stool, and cerebrospinal fluid has resulted in a paradigm shift in clinical microbiology and clinical practice. This review focuses on U.S. Food and Drug Administration (FDA)-approved/cleared multiplex molecular panels with more than five targets designed to assist in the diagnosis of bloodstream, respiratory tract, gastrointestinal, or central nervous system infections. While these panel-based assays have the clear advantages of a rapid turnaround time and the detection of a large number of microorganisms and promise to improve health care, they present certain challenges, including cost and the definition of ideal test utilization strategies (i.e., optimal ordering) and test interpretation.
MeSH term(s)	Humans ; Infection/diagnosis ; Molecular Diagnostic Techniques/methods ; Molecular Diagnostic Techniques/standards ; Molecular Diagnostic Techniques/trends ; United States ; United States Food and Drug Administration
Keywords	covid19
Language	English
Publishing date	2017-11-15
Publishing country	United States
Document type	Journal Article ; Review
ZDB-ID	645015-5
ISSN	1098-6618 ; 0893-8512
ISSN (online)	1098-6618
ISSN	0893-8512
DOI	10.1128/CMR.00024-17
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 2409: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Fungal Diagnostic Stewardship in Bronchoscopy Specimens for Immunocompetent Patients in the Intensive Care Unit.

Shah, Aditya S / O'Horo, John C / Tang, Schirin / Bryson, Alexandra L / Wengenack, Nancy L / Sampathkumar, Priya

Mayo Clinic proceedings

2019 Volume 94, Issue 9, Page(s) 1781–1785

Abstract: Objective: To evaluate the diagnostic yield of fungal smears and cultures from bronchial lavage and wash specimens obtained from immunocompetent patients in the intensive care unit (ICU) because respiratory tract samples from patients in the ICU often ... ...

Abstract	Objective: To evaluate the diagnostic yield of fungal smears and cultures from bronchial lavage and wash specimens obtained from immunocompetent patients in the intensive care unit (ICU) because respiratory tract samples from patients in the ICU often undergo extensive microbiological testing. Patients and methods: In total, we enrolled 112 immunocompetent adult patients treated in the medical and surgical ICU between July 1, 2016, and June 30, 2017. We evaluated whether the results of fungal smears and cultures of specimens obtained from bronchoscopy and bronchoalveolar lavage changed patient care. Results: In total, 131 bronchoscopic specimens and 31 bronchoalveolar lavage specimens were tested for fungi. Cultures were held for an estimated 4680 culture-days. Two results changed patient therapy. In both cases, other routine tests provided the same information as fungal culture before these results were returned. Conclusion: In immunocompetent, critically ill patients, fungal culture of respiratory tract specimens does not add diagnostic value. Routine fungal culture of respiratory tract specimens should be discouraged in this population.
MeSH term(s)	Adult ; Antifungal Agents/therapeutic use ; Bronchoalveolar Lavage/methods ; Bronchoalveolar Lavage Fluid/microbiology ; Bronchoscopy/methods ; Cohort Studies ; Critical Illness ; Culture Techniques ; Female ; Fungi/isolation & purification ; Humans ; Intensive Care Units ; Male ; Middle Aged ; Prognosis ; Respiratory Tract Infections/diagnosis ; Respiratory Tract Infections/drug therapy ; Respiratory Tract Infections/immunology ; Respiratory Tract Infections/microbiology ; Retrospective Studies ; Risk Assessment ; Treatment Outcome ; Unnecessary Procedures/methods
Chemical Substances	Antifungal Agents
Language	English
Publishing date	2019-08-07
Publishing country	England
Document type	Journal Article
ZDB-ID	124027-4
ISSN	1942-5546 ; 0025-6196
ISSN (online)	1942-5546
ISSN	0025-6196
DOI	10.1016/j.mayocp.2019.02.020
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Ua VI Zs.183: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Covalent Modification of Bacteriophage T4 DNA Inhibits CRISPR-Cas9.

Bryson, Alexandra L / Hwang, Young / Sherrill-Mix, Scott / Wu, Gary D / Lewis, James D / Black, Lindsay / Clark, Tyson A / Bushman, Frederic D

mBio

2015 Volume 6, Issue 3, Page(s) e00648

Abstract: Unlabelled: The genomic DNAs of tailed bacteriophages are commonly modified by the attachment of chemical groups. Some forms of DNA modification are known to protect phage DNA from cleavage by restriction enzymes, but others are of unknown function. ... ...

Abstract	Unlabelled: The genomic DNAs of tailed bacteriophages are commonly modified by the attachment of chemical groups. Some forms of DNA modification are known to protect phage DNA from cleavage by restriction enzymes, but others are of unknown function. Recently, the CRISPR-Cas nuclease complexes were shown to mediate bacterial adaptive immunity by RNA-guided target recognition, raising the question of whether phage DNA modifications may also block attack by CRISPR-Cas9. We investigated phage T4 as a model system, where cytosine is replaced with glucosyl-hydroxymethylcytosine (glc-HMC). We first quantified the extent and distribution of covalent modifications in T4 DNA by single-molecule DNA sequencing and enzymatic probing. We then designed CRISPR spacer sequences targeting T4 and found that wild-type T4 containing glc-HMC was insensitive to attack by CRISPR-Cas9 but mutants with unmodified cytosine were sensitive. Phage with HMC showed only intermediate sensitivity. While this work was in progress, another group reported examples of heavily engineered CRISRP-Cas9 complexes that could, in fact, overcome the effects of T4 DNA modification, indicating that modifications can inhibit but do not always fully block attack. Importance: Bacteria were recently found to have a form of adaptive immunity, the CRISPR-Cas systems, which use nucleic acid pairing to recognize and cleave genomic DNA of invaders such as bacteriophage. Historic work with tailed phages has shown that phage DNA is often modified by covalent attachment of large chemical groups. Here we demonstrate that DNA modification in phage T4 inhibits attack by the CRISPR-Cas9 system. This finding provides insight into mechanisms of host-virus competition and also a new set of tools that may be useful in modulating the activity of CRISPR-Cas9 in genome engineering applications.
MeSH term(s)	Bacteriophage T4/genetics ; Bacteriophage T4/growth & development ; CRISPR-Cas Systems ; Cytosine/chemistry ; Cytosine/metabolism ; DNA, Viral/chemistry ; DNA, Viral/genetics ; DNA, Viral/metabolism ; Escherichia coli/virology ; Microbial Viability ; Molecular Sequence Data ; Sequence Analysis, DNA ; Viral Plaque Assay
Chemical Substances	DNA, Viral ; Cytosine (8J337D1HZY)
Language	English
Publishing date	2015-06-16
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural
ZDB-ID	2557172-2
ISSN	2150-7511 ; 2161-2129
ISSN (online)	2150-7511
ISSN	2161-2129
DOI	10.1128/mBio.00648-15
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article: Covalent Modification of Bacteriophage T4 DNA Inhibits CRISPR-Cas9

Bryson, Alexandra L / Hwang, Young / Sherrill-Mix, Scott / Wu, Gary D / Lewis, James D / Black, Lindsay / Clark, Tyson A / Bushman, Frederic D

mBio. 2015 July 1, v. 6, no. 3

2015

Abstract: ABSTRACT The genomic DNAs of tailed bacteriophages are commonly modified by the attachment of chemical groups. Some forms of DNA modification are known to protect phage DNA from cleavage by restriction enzymes, but others are of unknown function. ... ...

Abstract	ABSTRACT The genomic DNAs of tailed bacteriophages are commonly modified by the attachment of chemical groups. Some forms of DNA modification are known to protect phage DNA from cleavage by restriction enzymes, but others are of unknown function. Recently, the CRISPR-Cas nuclease complexes were shown to mediate bacterial adaptive immunity by RNA-guided target recognition, raising the question of whether phage DNA modifications may also block attack by CRISPR-Cas9. We investigated phage T4 as a model system, where cytosine is replaced with glucosyl-hydroxymethylcytosine (glc-HMC). We first quantified the extent and distribution of covalent modifications in T4 DNA by single-molecule DNA sequencing and enzymatic probing. We then designed CRISPR spacer sequences targeting T4 and found that wild-type T4 containing glc-HMC was insensitive to attack by CRISPR-Cas9 but mutants with unmodified cytosine were sensitive. Phage with HMC showed only intermediate sensitivity. While this work was in progress, another group reported examples of heavily engineered CRISRP-Cas9 complexes that could, in fact, overcome the effects of T4 DNA modification, indicating that modifications can inhibit but do not always fully block attack.
Keywords	Caudovirales ; DNA ; adaptive immunity ; bacteria ; bacteriophages ; cytosine ; genetic engineering ; mutants ; restriction endonucleases ; sequence analysis
Language	English
Dates of publication	2015-0701
Size	p. e00648-15.
Publishing place	American Society for Microbiology
Document type	Article
ZDB-ID	2557172-2
ISSN	2150-7511 ; 2161-2129
ISSN (online)	2150-7511
ISSN	2161-2129
DOI	10.1128/mBio.00648-15
Database	NAL-Catalogue (AGRICOLA)

Order via subito

To top

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito