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Article: Evaluation of Different Procedures for Titanium Dental Implant Surface Decontamination-In Vitro Study.

Jordan, Ante / Smojver, Igor / Budimir, Ana / Gabrić, Dragana / Vuletić, Marko

2024 Volume 11, Issue 4

Abstract: Polymicrobial biofilm removal and decontamination of the implant surface is the most important goal in the treatment of periimplantitis. The aim of this study is to evaluate the efficacy of four different decontamination methods for ... ...

Abstract	Polymicrobial biofilm removal and decontamination of the implant surface is the most important goal in the treatment of periimplantitis. The aim of this study is to evaluate the efficacy of four different decontamination methods for removing
Language	English
Publishing date	2024-03-28
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2746191-9
ISSN	2306-5354
ISSN	2306-5354
DOI	10.3390/bioengineering11040326
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Article ; Online: MRSA in Croatia: prevalence and management.

Budimir, Ana

Expert review of anti-infective therapy

2016 Volume 14, Issue 2, Page(s) 167–176

Abstract: Infections caused by methicillin-resistant Staphylococcus aureus (MRSA) are associated with increased morbidity, mortality and length of hospital stay. MRSA is a major pathogen in hospitals and an important pathogen in community infections with few ... ...

Abstract	Infections caused by methicillin-resistant Staphylococcus aureus (MRSA) are associated with increased morbidity, mortality and length of hospital stay. MRSA is a major pathogen in hospitals and an important pathogen in community infections with few severe and fatal cases. However, MRSA causes the majority of skin and soft tissue infections in the US. The burden of community MRSA is much smaller in Europe, but there are reports of livestock-associated MRSA (LA-MRSA) isolated from pigs and cattle causing significant infections in the people who are connected to these farms. MRSA has been present in Croatia for more than 45 years, and it exerts a different impact on health-care infections. A remarkable increase in MRSA percentage was noted in primarily sterile samples in 2002 (37%) in comparison to 2001 (31%). This percentage remained quite high until 2008, when the first signs of a reduced trend were observed. The lowest percentage was 22% in 2012.
MeSH term(s)	Animals ; Anti-Bacterial Agents/therapeutic use ; Carrier State/epidemiology ; Carrier State/microbiology ; Cattle ; Croatia/epidemiology ; Daptomycin/therapeutic use ; Humans ; Linezolid/therapeutic use ; Livestock ; Methicillin-Resistant Staphylococcus aureus ; Minocycline/analogs & derivatives ; Minocycline/therapeutic use ; Prevalence ; Soft Tissue Infections/drug therapy ; Soft Tissue Infections/epidemiology ; Soft Tissue Infections/microbiology ; Staphylococcal Infections/drug therapy ; Staphylococcal Infections/epidemiology ; Staphylococcal Infections/microbiology ; Staphylococcal Skin Infections/drug therapy ; Staphylococcal Skin Infections/epidemiology ; Staphylococcal Skin Infections/microbiology ; Swine ; Teicoplanin/therapeutic use ; Vancomycin/therapeutic use ; Zoonoses/drug therapy ; Zoonoses/epidemiology ; Zoonoses/microbiology
Chemical Substances	Anti-Bacterial Agents ; Teicoplanin (61036-62-2) ; Vancomycin (6Q205EH1VU) ; tigecycline (70JE2N95KR) ; Minocycline (FYY3R43WGO) ; Linezolid (ISQ9I6J12J) ; Daptomycin (NWQ5N31VKK)
Language	English
Publishing date	2016
Publishing country	England
Document type	Journal Article ; Review
ZDB-ID	2181279-2
ISSN	1744-8336 ; 1478-7210
ISSN (online)	1744-8336
ISSN	1478-7210
DOI	10.1586/14787210.2016.1116384
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Evaluation of the Photoactivation Effect of 3% Hydrogen Peroxide in the Disinfection of Dental Implants: In Vitro Study.

Katalinić, Ivan / Smojver, Igor / Morelato, Luka / Vuletić, Marko / Budimir, Ana / Gabrić, Dragana

Biomedicines

2023 Volume 11, Issue 4

Abstract: Photoactivation of 3% hydrogen peroxide with a 445 nm diode laser represents a relatively new, insufficiently researched antimicrobial method in the treatment of peri-implantitis. The purpose of this work is to evaluate the effect of photoactivation of 3% ...

Abstract	Photoactivation of 3% hydrogen peroxide with a 445 nm diode laser represents a relatively new, insufficiently researched antimicrobial method in the treatment of peri-implantitis. The purpose of this work is to evaluate the effect of photoactivation of 3% hydrogen peroxide with a 445 nm diode laser, and to compare the obtained results with 0.2% chlorhexidine treatment and 3% hydrogen peroxide treatment without photoactivation, in vitro, on the surface of dental implants contaminated with
Language	English
Publishing date	2023-03-24
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2720867-9
ISSN	2227-9059
ISSN	2227-9059
DOI	10.3390/biomedicines11041002
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Article ; Online: Metal ions, Alzheimer's disease and chelation therapy.

Budimir, Ana

Acta pharmaceutica (Zagreb, Croatia)

2011 Volume 61, Issue 1, Page(s) 1–14

Abstract: In the last few years, various studies have been providing evidence that metal ions are critically involved in the pathogenesis of major neurological diseases (Alzheimer, Parkinson). Metal ion chelators have been suggested as potential therapies for ... ...

Abstract	In the last few years, various studies have been providing evidence that metal ions are critically involved in the pathogenesis of major neurological diseases (Alzheimer, Parkinson). Metal ion chelators have been suggested as potential therapies for diseases involving metal ion imbalance. Neurodegeneration is an excellent target for exploiting the metal chelator approach to therapeutics. In contrast to the direct chelation approach in metal ion overload disorders, in neurodegeneration the goal seems to be a better and subtle modulation of metal ion homeostasis, aimed at restoring ionic balance. Thus, moderate chelators able to coordinate deleterious metals without disturbing metal homeostasis are needed. To date, several chelating agents have been investigated for their potential to treat neurodegeneration, and a series of 8-hydroxyquinoline analogues showed the greatest potential for the treatment of neurodegenerative diseases.
MeSH term(s)	Alzheimer Disease/drug therapy ; Alzheimer Disease/physiopathology ; Animals ; Chelating Agents/pharmacology ; Homeostasis/drug effects ; Humans ; Hydroxyquinolines/chemistry ; Hydroxyquinolines/pharmacology ; Ions/metabolism ; Metals/metabolism ; Neurodegenerative Diseases/drug therapy ; Neurodegenerative Diseases/physiopathology
Chemical Substances	Chelating Agents ; Hydroxyquinolines ; Ions ; Metals
Language	English
Publishing date	2011-03
Publishing country	Croatia
Document type	Journal Article ; Review
ZDB-ID	1111806-4
ISSN	1846-9558 ; 1330-0075
ISSN (online)	1846-9558
ISSN	1330-0075
DOI	10.2478/v10007-011-0006-6
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Article: Sepsityper

Perše, Gabrijela / Samošćanec, Ivana / Bošnjak, Zrinka / Budimir, Ana / Kuliš, Tomislav / Mareković, Ivana

Life (Basel, Switzerland)

2022 Volume 12, Issue 11

Abstract: In order to further accelerate pathogen identification from positive blood cultures (BC), various sample preparation protocols to identify bacteria with MALDI-TOF MS directly from positive BCs have been developed. We evaluated an in-house method in ... ...

Abstract	In order to further accelerate pathogen identification from positive blood cultures (BC), various sample preparation protocols to identify bacteria with MALDI-TOF MS directly from positive BCs have been developed. We evaluated an in-house method in comparison to the Sepsityper® Kit (Bruker Daltonics, Bremen, Germany) as well as the benefit of an on-plate formic acid extraction step following positive signal by the BACTECTM FX system. Confirmation of identification was achieved using subcultured growing biomass used for MALDI-TOF MS analysis. A total of 113 monomicrobial positive BCs were analyzed. The rates of Gram-positive bacteria correctly identified to the genus level using in-house method and Sepsityper® Kit were 63.3% (38/60) and 81.7% (49/60), respectively (p = 0.025). Identification rates at species level for Gram-positive bacteria with in-house method and Sepsityper® kit were 30.0% (18/60) and 66.7% (40/60), respectively (p < 0.001). Identification rates of Gram-negative bacteria were similar with the in-house method and Sepsityper® Kit. Additional on-plate formic acid extraction demonstrated significant improvement in the identification rate of Gram-positive bacteria at both genus and species level for both in-house (p = 0.001, p < 0.001) and Sepsityper® Kit methods (p = 0.007, p < 0.001). Our in-house method is a candidate for laboratory routines with Sepsityper® Kit as a back-up solution when identification of Gram-positive bacteria is unsuccessful.
Language	English
Publishing date	2022-10-30
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2662250-6
ISSN	2075-1729
ISSN	2075-1729
DOI	10.3390/life12111744
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Article: Sealing Ability of Bioactive Root-End Filling Materials in Retro Cavities Prepared with Er,Cr:YSGG Laser and Ultrasonic Techniques.

Bago, Ivona / Lucić, Ružica / Budimir, Ana / Rajić, Valentina / Balić, Merima / Anić, Ivica

Bioengineering (Basel, Switzerland)

2022 Volume 9, Issue 7

Abstract: The aim of this in vitro study was to compare the apical sealing ability of total fill bioceramic root repair material (BC-RRM) and mineral trioxide aggregate (MTA), regarding the retrograde preparation technique used: ultrasonic or erbium, chromium: ... ...

Abstract	The aim of this in vitro study was to compare the apical sealing ability of total fill bioceramic root repair material (BC-RRM) and mineral trioxide aggregate (MTA), regarding the retrograde preparation technique used: ultrasonic or erbium, chromium: yttrium, scandium, gallium, or garnet (Er,Cr:YSGG) laser. The study sample consisted of 48 human single-rooted teeth. After root-end resection, the samples were divided into two groups, according to the retrograde preparation technique used: Group 1: ultrasonic; Group 2: Er,Cr:YSGG laser. In each group, half of the retrograde cavities were filled with BC-RRM, and the other half were filled with MTA. The specimens were mounted in tubes and sterilized in plasma. The root canals were inoculated with Enterococcus faecalis, and the tubes were filled with fetal bovine serum, leaving the apical part of the root in the serum. After 30 days, the canals were sampled and cultured, and the colony forming units (CFUs) were counted with the additional polymerase chain reaction (PCR analysis). There was no significant difference between ultrasonic groups and the Er,Cr:YSGG-MTA group, regarding the number of CFUs (p > 0.05). The Er,Cr:YSGG-BC-RRM group showed the highest number of remaining viable bacteria (p < 0.001). Both filling materials filled in ultrasonic preparations presented similar sealing abilities. The BC-RRM showed more leakage when used in retro cavities prepared with the Er,Cr:YSGG laser.
Language	English
Publishing date	2022-07-14
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2746191-9
ISSN	2306-5354
ISSN	2306-5354
DOI	10.3390/bioengineering9070314
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Article: A Novel Technique for Disinfection Treatment of Contaminated Dental Implant Surface Using 0.1% Riboflavin and 445 nm Diode Laser-An In Vitro Study.

Morelato, Luka / Budimir, Ana / Smojver, Igor / Katalinić, Ivan / Vuletić, Marko / Ajanović, Muhamed / Gabrić, Dragana

Bioengineering (Basel, Switzerland)

2022 Volume 9, Issue 7

Abstract: Background: Antimicrobial photodynamic therapy (PDT) has been introduced as a potential option for peri-implantitis treatment. The aim of this study is to evaluate the antimicrobial effect of a novel technique involving a combination of 445 nm diode ... ...

Abstract	Background: Antimicrobial photodynamic therapy (PDT) has been introduced as a potential option for peri-implantitis treatment. The aim of this study is to evaluate the antimicrobial effect of a novel technique involving a combination of 445 nm diode laser light with 0.1% riboflavin solution (used as a photosensitizing dye) as applied on a bacterial-fungal biofilm formed on implants and to compare the performance of this technique with that of the commonly used combination of 660 nm diode laser with 0.1% methylene blue dye. Methods: An in vitro study was conducted on 80 titanium dental implants contaminated with Results: The PDT1 and PDT2 groups showed greater reduction of SA and CA in comparison to the NC group and no significant differences in comparison to the PC group. No statistically significant differences between the PDT1 and PDT2 groups were observed. Conclusions: A novel antimicrobial treatment involving a combination of 445 nm diode laser light with riboflavin solution showed efficiency in reducing SA and CA biofilm formation on dental implant surfaces comparable to those of the more commonly used PDT treatment consisting of 660 nm diode laser light with methylene blue dye or 0.2% CHX treatment.
Language	English
Publishing date	2022-07-12
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2746191-9
ISSN	2306-5354
ISSN	2306-5354
DOI	10.3390/bioengineering9070308
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Article: Sealing Efficacy of the Original and Third-Party Custom-Made Abutments-Microbiological In Vitro Pilot Study.

Smojver, Igor / Bjelica, Roko / Ćatić, Amir / Budimir, Ana / Vuletić, Marko / Gabrić, Dragana

Materials (Basel, Switzerland)

2022 Volume 15, Issue 4

Abstract: Implant-abutment connection (IAC) is a key factor for the long-term success and stability of implant-supported prosthodontic restoration and its surrounding tissues. Misfit between prosthodontic abutment and implant at the IAC leads to technical and ... ...

Abstract	Implant-abutment connection (IAC) is a key factor for the long-term success and stability of implant-supported prosthodontic restoration and its surrounding tissues. Misfit between prosthodontic abutment and implant at the IAC leads to technical and biological complications. Two kinds of prosthodontic abutments are currently available on the market: original and third-party abutments. The aim of this pilot study was to test and compare the internal fit (gap) at the implant-abutment interface depending on the abutment fabrication method based on microbial leakage in static conditions and the need for the use of gap sealing material. Two groups of 40 implants were formed on the basis of the type of abutment. In each of the groups of two implant systems, two subgroups of 10 implants were formed. The tested subgroups consisted of 10 implants with sealing material and a negative control subgroups consisting of 10 implants without any sealing material. The test material, GapSeal (Hager and Werken, Duisburg, Germany) was applied in the test subgroups. The implant-abutment assemblies were contaminated with a solution containing
Language	English
Publishing date	2022-02-21
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2487261-1
ISSN	1996-1944
ISSN	1996-1944
DOI	10.3390/ma15041597
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Antimicrobial Efficacy and Permeability of Various Sealing Materials in Two Different Types of Implant-Abutment Connections.

Smojver, Igor / Bjelica, Roko / Vuletić, Marko / Gerbl, Dražena / Budimir, Ana / Gabrić, Dragana

International journal of molecular sciences

2022 Volume 23, Issue 14

Abstract: The presence of a microgap along an implant-abutment connection (IAC) is considered the main disadvantage of two-piece implant systems. Its existence may lead to mechanical and biological complications. Different IAC designs have been developed to ... ...

Abstract	The presence of a microgap along an implant-abutment connection (IAC) is considered the main disadvantage of two-piece implant systems. Its existence may lead to mechanical and biological complications. Different IAC designs have been developed to minimise microleakage through the microgap and to increase the stability of prosthodontic abutments. Furthermore, different sealing materials have appeared on the market to seal the gap at the IAC. The purpose of this study was to evaluate the antimicrobial efficacy and permeability of different materials designed to seal the microgap, and their behaviour in conical and straight types of internal IACs. One hundred dental implants with original prosthodontic abutments were divided into two groups of fifty implants according to the type of IAC. Three different sealing materials (GapSeal, Flow.sil, and Oxysafe gel) were applied in the test subgroups. The contamination of implant-abutment assemblies was performed by a joint suspension containing
MeSH term(s)	Anti-Bacterial Agents ; Dental Implants ; Materials Testing ; Permeability ; Prostheses and Implants ; Staphylococcus aureus
Chemical Substances	Anti-Bacterial Agents ; Dental Implants
Language	English
Publishing date	2022-07-21
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms23148031
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Environmentally Benign Phytic Acid-Based Nanocoating for Multifunctional Flame-Retardant/Antibacterial Cotton

Magovac, Eva / Vončina, Bojana / Budimir, Ana / Jordanov, Igor / Grunlan, Jaime C. / Bischof, Sandra

Fibers. 2021 Oct. 31, v. 9, no. 11

2021

Abstract: Environmentally benign layer-by-layer (LbL) deposition was used to obtain flame-retardant and antimicrobial cotton. Cotton was coated with 8, 10, and 12 phytic acid (PA) and chitosan (CH)-urea bilayers (BL) and then immersed into copper (II) sulfate ( ... ...

Abstract	Environmentally benign layer-by-layer (LbL) deposition was used to obtain flame-retardant and antimicrobial cotton. Cotton was coated with 8, 10, and 12 phytic acid (PA) and chitosan (CH)-urea bilayers (BL) and then immersed into copper (II) sulfate (CuSO₄) solution. Our findings were that 12 BL of PA/CH-urea + Cu²⁺ were able to stop flame on cotton during vertical flammability testing (VFT) with a limiting oxygen index (LOI) value of 26%. Microscale combustion calorimeter (MCC) data showed a reduction of peak heat release rates (pHRR) of more than 61%, while the reduction of total heat release (THR) was more than 54%, relative to untreated cotton. TG-IR analysis of 12 BL-treated cotton showed the release of water, methane, carbon dioxide, carbon monoxide, and aldehydes, while by adding Cu²⁺ ions, the treated cotton produces a lower amount of methane. Treated cotton also showed no levoglucosan. The intumescent behavior of the treatment was indicated by the bubbled structure of the post-burn char. Antibacterial testing showed a 100% reduction of Klebsiella pneumoniae and Staphylococcus aureus. In this study, cotton was successfully functionalized with a multifunctional ecologically benign flame-retardant and antibacterial nanocoating, by means of LbL deposition.
Keywords	Klebsiella pneumoniae ; Staphylococcus aureus ; calorimeters ; carbon dioxide ; carbon monoxide ; chitosan ; combustion ; copper ; cotton ; flammability ; heat ; methane ; oxygen ; phytic acid
Language	English
Dates of publication	2021-1031
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article
ZDB-ID	2723193-8
ISSN	2079-6439
ISSN	2079-6439
DOI	10.3390/fib9110069
Database	NAL-Catalogue (AGRICOLA)

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