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  1. Article ; Online: Transcriptomics reveals a distinct metabolic profile in T cells from severe allergic asthmatic patients.

    Pablo-Torres, Carmela / Garcia-Escribano, Carlota / Romeo, Martina / Gomez-Casado, Cristina / Arroyo Solera, Ricardo / Bueno-Cabrera, José Luis / Del Mar Reaño Martos, M / Iglesias-Cadarso, Alfredo / Tarín, Carlos / Agache, Ioana / Chivato, Tomás / Barber, Domingo / Escribese, María M / Izquierdo, Elena

    Frontiers in allergy

    2023  Volume 4, Page(s) 1129248

    Abstract: The reasons behind the onset and continuation of chronic inflammation in individuals with severe allergies are still not understood. Earlier findings indicated that there is a connection between severe allergic inflammation, systemic metabolic ... ...

    Abstract The reasons behind the onset and continuation of chronic inflammation in individuals with severe allergies are still not understood. Earlier findings indicated that there is a connection between severe allergic inflammation, systemic metabolic alterations and impairment of regulatory functions. Here, we aimed to identify transcriptomic alterations in T cells associated with the degree of severity in allergic asthmatic patients. T cells were isolated from severe (
    Language English
    Publishing date 2023-05-31
    Publishing country Switzerland
    Document type Journal Article
    ISSN 2673-6101
    ISSN (online) 2673-6101
    DOI 10.3389/falgy.2023.1129248
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Deciphering the role of platelets in severe allergy by an integrative omics approach

    Pablo‐Torres, Carmela / Izquierdo, Elena / Tan, Tiak Ju / Obeso, David / Layhadi, Janice A. / Sánchez‐Solares, Javier / Mera‐Berriatua, Leticia / Bueno‐Cabrera, José Luis / del Mar Reaño‐Martos, María / Iglesias‐Cadarso, Alfredo / Barbas, Coral / Gomez‐Casado, Cristina / Villaseñor, Alma / Barber, Domingo / Shamji, Mohamed H. / Escribese, María M.

    Allergy. 2023 May, v. 78, no. 5, p. 1319-1332

    2023  , Page(s) 1319–1332

    Abstract: BACKGROUND: Mechanisms causing the onset and perpetuation of inflammation in severe allergic patients remain unknown. Our previous studies suggested that severe allergic inflammation is linked to platelet dysfunction. METHODS: Platelet‐rich plasma (PRP) ... ...

    Abstract BACKGROUND: Mechanisms causing the onset and perpetuation of inflammation in severe allergic patients remain unknown. Our previous studies suggested that severe allergic inflammation is linked to platelet dysfunction. METHODS: Platelet‐rich plasma (PRP) and platelet‐poor plasma (PPP) samples were obtained by platelet‐apheresis from severe (n = 7) and mild (n = 10) allergic patients and nonallergic subjects (n = 9) to perform platelet lipidomics by liquid chromatography coupled to mass spectrometry (LC–MS) and RNA‐seq analysis. Significant metabolites and transcripts were used to identify compromised biological pathways in the severe phenotype. Platelet and inflammation‐related proteins were quantified by Luminex. RESULTS: Platelets from severe allergic patients were characterized by high levels of ceramides, phosphoinositols, phosphocholines, and sphingomyelins. In contrast, they showed a decrease in eicosanoid precursor levels. Biological pathway analysis performed with the significant lipids revealed the alteration of phospholipases, calcium‐dependent events, and linolenic metabolism. RNAseq confirmed mRNA overexpression of genes related to platelet activation and arachidonic acid metabolism in the severe phenotypes. Pathway analysis indicated the alteration of NOD, MAPK, TLR, TNF, and IL‐17 pathways in the severe phenotype. P‐Selectin and IL‐17AF proteins were increased in the severe phenotype. CONCLUSIONS: This study demonstrates that platelet lipid, mRNA, and protein content is different according to allergy severity. These findings suggest that platelet load is a potential source of biomarkers and a new chance for therapeutic targets in severe inflammatory pathologies.
    Keywords arachidonic acid ; biomarkers ; ceramides ; hypersensitivity ; inflammation ; interleukin-17 ; lipidomics ; liquid chromatography ; mass spectrometry ; metabolism ; metabolites ; multiomics ; phenotype ; phospholipases ; platelet activation ; protein content ; sequence analysis ; sphingomyelins ; therapeutics
    Language English
    Dates of publication 2023-05
    Size p. 1319-1332
    Publishing place John Wiley & Sons, Ltd
    Document type Article ; Online
    Note JOURNAL ARTICLE
    ZDB-ID 391933-x
    ISSN 1398-9995 ; 0105-4538
    ISSN (online) 1398-9995
    ISSN 0105-4538
    DOI 10.1111/all.15621
    Database NAL-Catalogue (AGRICOLA)

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  3. Article ; Online: Deciphering the role of platelets in severe allergy by an integrative omics approach.

    Pablo-Torres, Carmela / Izquierdo, Elena / Tan, Tiak Ju / Obeso, David / Layhadi, Janice A / Sánchez-Solares, Javier / Mera-Berriatua, Leticia / Bueno-Cabrera, José Luis / Del Mar Reaño-Martos, María / Iglesias-Cadarso, Alfredo / Barbas, Coral / Gomez-Casado, Cristina / Villaseñor, Alma / Barber, Domingo / Shamji, Mohamed H / Escribese, María M

    Allergy

    2023  Volume 78, Issue 5, Page(s) 1319–1332

    Abstract: Background: Mechanisms causing the onset and perpetuation of inflammation in severe allergic patients remain unknown. Our previous studies suggested that severe allergic inflammation is linked to platelet dysfunction.: Methods: Platelet-rich plasma ( ... ...

    Abstract Background: Mechanisms causing the onset and perpetuation of inflammation in severe allergic patients remain unknown. Our previous studies suggested that severe allergic inflammation is linked to platelet dysfunction.
    Methods: Platelet-rich plasma (PRP) and platelet-poor plasma (PPP) samples were obtained by platelet-apheresis from severe (n = 7) and mild (n = 10) allergic patients and nonallergic subjects (n = 9) to perform platelet lipidomics by liquid chromatography coupled to mass spectrometry (LC-MS) and RNA-seq analysis. Significant metabolites and transcripts were used to identify compromised biological pathways in the severe phenotype. Platelet and inflammation-related proteins were quantified by Luminex.
    Results: Platelets from severe allergic patients were characterized by high levels of ceramides, phosphoinositols, phosphocholines, and sphingomyelins. In contrast, they showed a decrease in eicosanoid precursor levels. Biological pathway analysis performed with the significant lipids revealed the alteration of phospholipases, calcium-dependent events, and linolenic metabolism. RNAseq confirmed mRNA overexpression of genes related to platelet activation and arachidonic acid metabolism in the severe phenotypes. Pathway analysis indicated the alteration of NOD, MAPK, TLR, TNF, and IL-17 pathways in the severe phenotype. P-Selectin and IL-17AF proteins were increased in the severe phenotype.
    Conclusions: This study demonstrates that platelet lipid, mRNA, and protein content is different according to allergy severity. These findings suggest that platelet load is a potential source of biomarkers and a new chance for therapeutic targets in severe inflammatory pathologies.
    MeSH term(s) Humans ; Blood Platelets/metabolism ; Phenotype ; Hypersensitivity/genetics ; Hypersensitivity/metabolism ; Inflammation/metabolism ; RNA, Messenger/metabolism
    Chemical Substances RNA, Messenger
    Language English
    Publishing date 2023-01-12
    Publishing country Denmark
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 391933-x
    ISSN 1398-9995 ; 0105-4538
    ISSN (online) 1398-9995
    ISSN 0105-4538
    DOI 10.1111/all.15621
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Blood Donations and Transfusions during the COVID-19 Pandemic in Spain: Impact According to Autonomous Communities and Hospitals.

    García-Erce, José Antonio / Romón-Alonso, Íñigo / Jericó, Carlos / Domingo-Morera, José María / Arroyo-Rodríguez, José Luis / Sola-Lapeña, Carlos / Bueno-Cabrera, José Luis / Juárez-Vela, Raúl / Zalba-Marcos, Saioa / Abad-Motos, Ane / Gea-Caballero, Vicente / Santolalla-Arnedo, Iván / Quintana-Díaz, Manuel

    International journal of environmental research and public health

    2021  Volume 18, Issue 7

    Abstract: Worldwide, the COVID-19 pandemic has caused a decline in blood donations, between 30% and 70% in some of the most affected countries. In Spain, during the initial eight weeks after the State of Emergency was decreed on 14 March 2020, in the weekly ... ...

    Abstract Worldwide, the COVID-19 pandemic has caused a decline in blood donations, between 30% and 70% in some of the most affected countries. In Spain, during the initial eight weeks after the State of Emergency was decreed on 14 March 2020, in the weekly reports of the Health Ministry, an average decrease of 20% was observed between 11 and week 25 compared with the 2018 donation. We aimed to investigate the impact of the COVID-19 pandemic on blood donations and blood distribution in four autonomous communities, and to explore the evolution of the consumption of blood components (BCs) in ten hospitals of six autonomous communities. We performed a prospective study of grouped cohorts on the donation and distribution of blood in four regional transfusion centers in four autonomous communities in Spain, and a retrospective study of the consumption of blood components in ten hospitals in six autonomous communities. Regarding donations, there was no significant decrease in donations, with differences between autonomous communities, which started between 1 and 15 March 2020 (-11%). The increase in donations in phase II (from 26 May 2020) stands out. Regarding consumption, there was a significant reduction in the consumption of packed red blood cells (RBCs) (24.5%), plasma (45.3%), and platelets (25.3%) in the central period (16 March-10 May). The reduction in the consumption of RBCs was significant in the period from 1-15 March. Conclusions: The COVID-19 pandemic has affected the donation and consumption of BCs.
    MeSH term(s) Blood Donors ; COVID-19 ; Hospitals ; Humans ; Pandemics ; Prospective Studies ; Retrospective Studies ; SARS-CoV-2 ; Spain/epidemiology
    Language English
    Publishing date 2021-03-27
    Publishing country Switzerland
    Document type Journal Article
    ISSN 1660-4601
    ISSN (online) 1660-4601
    DOI 10.3390/ijerph18073480
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  5. Article ; Online: Factors related to the development of high antibody titres against SARS-CoV-2 in convalescent plasma donors from the ConPlas-19 trial.

    Romera Martínez, Irene / Avendaño-Solá, Cristina / Villegas Da Ros, Carolina / Bosch Llobet, Alba / García Erce, José Antonio / González Fraile, María Isabel / Guerra Domínguez, Luisa / Vicuña Andrés, Isabel / Anguita Velasco, Javier / González Rodríguez, Victoria Paz / Contreras, Enric / Urcelay Uranga, Sabin / Pajares Herraiz, Ángel Luis / Jimenez-Marco, Teresa / Ojea Pérez, Ana María / Arroyo Rodríguez, José Luis / Pérez-Olmeda, Mayte / Ramos-Martínez, Antonio / Velasco-Iglesias, Ana /
    Bueno Cabrera, José Luis / Duarte, Rafael F

    Vox sanguinis

    2023  Volume 119, Issue 1, Page(s) 27–33

    Abstract: Background and objectives: The efficacy of COVID-19 convalescent plasma (CP) associates with high titres of antibodies. ConPlas-19 clinical trial showed that CP reduces the risk of progression to severe COVID-19 at 28 days. Here, we aim to study ConPlas- ...

    Abstract Background and objectives: The efficacy of COVID-19 convalescent plasma (CP) associates with high titres of antibodies. ConPlas-19 clinical trial showed that CP reduces the risk of progression to severe COVID-19 at 28 days. Here, we aim to study ConPlas-19 donors and characteristics that associate with high anti-SARS-CoV-2 antibody levels.
    Materials and methods: Four-hundred donors were enrolled in ConPlas-19. The presence and titres of anti-SARS-CoV-2 antibodies were evaluated by EUROIMMUN anti-SARS-CoV-2 S1 IgG ELISA.
    Results: A majority of 80.3% of ConPlas-19 donor candidates had positive EUROIMMUN test results (ratio ≥1.1), and of these, 51.4% had high antibody titres (ratio ≥3.5). Antibody levels decline over time, but nevertheless, out of 37 donors tested for an intended second CP donation, over 90% were still EUROIMMUN positive, and nearly 75% of those with high titres maintained high titres in the second sample. Donors with a greater probability of developing high titres of anti-SARS-CoV-2 antibodies include those older than 40 years of age (RR 2.06; 95% CI 1.24-3.42), with more than 7 days of COVID-19 symptoms (RR 1.89; 95% CI 1.05-3.43) and collected within 4 months from infection (RR 2.61; 95% CI 1.16-5.90). Male donors had a trend towards higher titres compared with women (RR 1.67; 95% CI 0.91-3.06).
    Conclusion: SARS-CoV-2 CP candidate donors' age, duration of COVID-19 symptoms and time from infection to donation associate with the collection of CP with high antibody levels. Beyond COVID-19, these data are relevant to inform decisions to optimize the CP donor selection process in potential future outbreaks.
    MeSH term(s) Female ; Humans ; Male ; Antibodies, Neutralizing ; Antibodies, Viral ; Blood Donors ; COVID-19/therapy ; COVID-19 Serotherapy ; Immunization, Passive/methods ; Immunoglobulin G ; SARS-CoV-2 ; Clinical Trials as Topic
    Chemical Substances Antibodies, Neutralizing ; Antibodies, Viral ; Immunoglobulin G
    Language English
    Publishing date 2023-11-20
    Publishing country England
    Document type Journal Article
    ZDB-ID 80313-3
    ISSN 1423-0410 ; 0042-9007
    ISSN (online) 1423-0410
    ISSN 0042-9007
    DOI 10.1111/vox.13561
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  6. Article ; Online: Pathogen reduction with methylene blue does not have an impact on the clinical effectiveness of COVID-19 convalescent plasma.

    Romera Martínez, Irene / Bueno Cabrera, José Luis / Domingo-Morera, José María / López Aguilar, Juan Carlos / Villegas Da Ros, Carolina / García Erce, José Antonio / Sáez Serrano, Isabel / Flores Ballester, Elena / Maglio, Laura Analía / Arbona Castaño, Cristina / Sola Lapeña, Carlos / Guerra Domínguez, Luisa / Berberana Fernández, Margarita / Madrigal Sánchez, María Elena / Rubio Batllés, Martín / Pérez-Olmeda, Mayte / Ramos-Martínez, Antonio / Velasco-Iglesias, Ana / Avendaño-Solá, Cristina /
    Duarte, Rafael F

    Vox sanguinis

    2023  Volume 118, Issue 4, Page(s) 296–300

    Abstract: Background and objectives: There is a concern about a possible deleterious effect of pathogen reduction (PR) with methylene blue (MB) on the function of immunoglobulins of COVID-19 convalescent plasma (CCP). We have evaluated whether MB-treated CCP is ... ...

    Abstract Background and objectives: There is a concern about a possible deleterious effect of pathogen reduction (PR) with methylene blue (MB) on the function of immunoglobulins of COVID-19 convalescent plasma (CCP). We have evaluated whether MB-treated CCP is associated with a poorer clinical response compared to other inactivation systems at the ConPlas-19 clinical trial.
    Materials and methods: This was an ad hoc sub-study of the ConPlas-19 clinical trial comparing the proportion of patients transfused with MB-treated CCP who had a worsening of respiration versus those treated with amotosalen (AM) or riboflavin (RB).
    Results: One-hundred and seventy-five inpatients with SARS-CoV-2 pneumonia were transfused with a single CCP unit. The inactivation system of the CCP units transfused was MB in 90 patients (51.4%), RB in 60 (34.3%) and AM in 25 (14.3%). Five out of 90 patients (5.6%) transfused with MB-treated CCP had worsening respiration compared to 9 out of 85 patients (10.6%) treated with alternative PR methods (p = 0.220). Of note, MB showed a trend towards a lower rate of respiratory progressions at 28 days (risk ratio, 0.52; 95% confidence interval, 0.18-1.50).
    Conclusion: Our data suggest that MB-treated CCP does not provide a worse clinical outcome compared to the other PR methods for the treatment of COVID-19.
    MeSH term(s) Humans ; COVID-19/therapy ; COVID-19 Serotherapy ; Immunization, Passive/methods ; Methylene Blue/pharmacology ; Methylene Blue/therapeutic use ; SARS-CoV-2 ; Treatment Outcome
    Chemical Substances Methylene Blue (T42P99266K)
    Language English
    Publishing date 2023-02-03
    Publishing country England
    Document type Clinical Trial ; Journal Article
    ZDB-ID 80313-3
    ISSN 1423-0410 ; 0042-9007
    ISSN (online) 1423-0410
    ISSN 0042-9007
    DOI 10.1111/vox.13406
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