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Article ; Online: Oligomeric State and Holding Activity of Hsp60.

Caruso Bavisotto, Celeste / Provenzano, Alessia / Passantino, Rosa / Marino Gammazza, Antonella / Cappello, Francesco / San Biagio, Pier Luigi / Bulone, Donatella

International journal of molecular sciences

2023 Volume 24, Issue 9

Abstract: Similar to its bacterial homolog GroEL, Hsp60 in oligomeric conformation is known to work as a folding machine, with the assistance of co-chaperonin Hsp10 and ATP. However, recent results have evidenced that Hsp60 can stabilize aggregation-prone ... ...

Abstract	Similar to its bacterial homolog GroEL, Hsp60 in oligomeric conformation is known to work as a folding machine, with the assistance of co-chaperonin Hsp10 and ATP. However, recent results have evidenced that Hsp60 can stabilize aggregation-prone molecules in the absence of Hsp10 and ATP by a different, "holding-like" mechanism. Here, we investigated the relationship between the oligomeric conformation of Hsp60 and its ability to inhibit fibrillization of the Ab40 peptide. The monomeric or tetradecameric form of the protein was isolated, and its effect on beta-amyloid aggregation was separately tested. The structural stability of the two forms of Hsp60 was also investigated using differential scanning calorimetry (DSC), light scattering, and circular dichroism. The results showed that the protein in monomeric form is less stable, but more effective against amyloid fibrillization. This greater functionality is attributed to the disordered nature of the domains involved in subunit contacts.
MeSH term(s)	Chaperonin 60/metabolism ; Adenosine Triphosphate/metabolism ; Chaperonin 10/chemistry ; Protein Folding
Chemical Substances	Chaperonin 60 ; Adenosine Triphosphate (8L70Q75FXE) ; Chaperonin 10
Language	English
Publishing date	2023-04-25
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms24097847
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Recombinant mussel protein Pvfp5β enhances cell adhesion of poly(vinyl alcohol)/k-carrageenan hydrogel scaffolds

Muscolino, Emanuela / Costa, Maria Assunta / Sabatino, Maria Antonietta / Alessi, Sabina / Bulone, Donatella / San Biagio, Pier Luigi / Passantino, Rosa / Giacomazza, Daniela / Dispenza, Clelia

International journal of biological macromolecules. 2022 June 30, v. 211

2022

Abstract: Polymeric hydrogels are increasingly considered as scaffolds for tissue engineering due to their extraordinary resemblance with the extracellular matrix (ECM) of many tissues. As cell adhesion is a key factor in regulating important cell functions, ... ...

Abstract	Polymeric hydrogels are increasingly considered as scaffolds for tissue engineering due to their extraordinary resemblance with the extracellular matrix (ECM) of many tissues. As cell adhesion is a key factor in regulating important cell functions, hydrogel scaffolds are often functionalized or loaded with a variety of bioactive molecules that can promote adhesion. Interesting biomimetic approaches exploit the properties of mussel-inspired recombinant adhesive proteins. In this work, we prepared hydrogel scaffolds with a 50%w mixture of k-carrageenan (kC) and polyvinyl alcohol (PVA), by a two-step physical gelation process, and we coated them with Perna viridis foot protein-5β (Pvfp5β). The mechanical and morphological properties of hydrogels were investigated both after conditioning with typical cell culture media and also after coating with the Pvfp5β. The protein resulted strongly adsorbed onto the surface of the hydrogel and also able to penetrate in its interiors to a certain depth, mainly interacting with the kC component of the scaffold as resulted from the confocal analysis. Mouse embryonic fibroblasts NIH-3T3 were seeded on top of the hydrogels and cultured up to two weeks. The role of Pvfp5β in promoting cell adhesion, spreading and colonization of the scaffold was demonstrated.
Keywords	Perna viridis ; adhesion ; biomimetics ; cell adhesion ; cell culture ; extracellular matrix ; fibroblasts ; gelation ; hydrogels ; mice ; mussels ; polyvinyl alcohol
Language	English
Dates of publication	2022-0630
Size	p. 639-652.
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	282732-3
ISSN	1879-0003 ; 0141-8130
ISSN (online)	1879-0003
ISSN	0141-8130
DOI	10.1016/j.ijbiomac.2022.05.068
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Article ; Online: The degree of compactness of the incipient High Methoxyl Pectin networks. A rheological insight at the sol-gel transition.

Ditta, Lorena Anna / Bulone, Donatella / Biagio, Pier Luigi San / Marino, Rosamaria / Giacomazza, Daniela / Lapasin, Romano

International journal of biological macromolecules

2020 Volume 158, Page(s) 985–993

Abstract: Fractal analysis can be properly applied to complex structures, like physical and chemical networks formed by particles or polymers, when they exhibit self-similarity over an extended range of length scales and, hence, can be profitably used not only for ...

Abstract	Fractal analysis can be properly applied to complex structures, like physical and chemical networks formed by particles or polymers, when they exhibit self-similarity over an extended range of length scales and, hence, can be profitably used not only for their morphological characterization but also for individuating possible relationships between morphology and mechanisms of aggregation and crosslinking, as well as between morphology and physical properties. Several experimental methods are available to determine the fractal dimension of gel networks, including various scattering techniques and microscopies, permeability measurements and rheology. The present study regards the self-assembly kinetics of High Methoxyl Pectin (HMP) solutions with different pectin and sucrose concentrations investigated by rheological measurements to highlight the effects of pectin and sucrose concentrations on the gel point and to evaluate the degree of compactness of the incipient gel networks through an interpretation of the viscoelastic response at the sol-gel transition.
Language	English
Publishing date	2020-05-06
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	282732-3
ISSN	1879-0003 ; 0141-8130
ISSN (online)	1879-0003
ISSN	0141-8130
DOI	10.1016/j.ijbiomac.2020.05.019
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Article ; Online: Synaptosomes: new vesicles for neuronal mitochondrial transplantation.

Picone, Pasquale / Porcelli, Gaetana / Bavisotto, Celeste Caruso / Nuzzo, Domenico / Galizzi, Giacoma / Biagio, Pier Luigi San / Bulone, Donatella / Di Carlo, Marta

Journal of nanobiotechnology

2021 Volume 19, Issue 1, Page(s) 6

Abstract: Background: Mitochondrial dysfunction is a critical factor in the onset and progression of neurodegenerative diseases. Recently, mitochondrial transplantation has been advised as an innovative and attractive strategy to transfer and replace damaged ... ...

Abstract	Background: Mitochondrial dysfunction is a critical factor in the onset and progression of neurodegenerative diseases. Recently, mitochondrial transplantation has been advised as an innovative and attractive strategy to transfer and replace damaged mitochondria. Here we propose, for the first time, to use rat brain extracted synaptosomes, a subcellular fraction of isolated synaptic terminal that contains mitochondria, as mitochondrial delivery systems. Results: Synaptosome preparation was validated by the presence of Synaptophysin and PSD95. Synaptosomes were characterized in terms of dimension, zeta potential, polydispersity index and number of particles/ml. Nile Red or CTX-FITCH labeled synaptosomes were internalized in LAN5 recipient cells by a mechanism involving specific protein-protein interaction, as demonstrated by loss of fusion ability after trypsin treatment and using different cell lines. The loading and release ability of the synaptosomes was proved by the presence of curcumin both into synaptosomes and LAN5 cells. The vitality of mitochondria transferred by Synaptosomes was demonstrated by the presence of Opa1, Fis1 and TOM40 mitochondrial proteins and JC-1 measurements. Further, synaptosomes deliver vital mitochondria into the cytoplasm of neuronal cells as demonstrated by microscopic images, increase of TOM 40, cytochrome c, Hexokinase II mitochondrial proteins, and presence of rat mitochondrial DNA. Finally, by using synaptosomes as a vehicle, healthy mitochondria restored mitochondrial function in cells containing rotenone or CCCp damaged mitochondria. Conclusions: Taken together these results suggest that synaptosomes can be a natural vehicle for the delivery of molecules and organelles to neuronal cells. Further, the replacement of affected mitochondria with healthy ones could be a potential therapy for treating neuronal mitochondrial dysfunction-related diseases.
MeSH term(s)	Animals ; Cytochromes c ; DNA, Mitochondrial ; Drug Delivery Systems ; Homeostasis ; Male ; Membrane Potentials ; Mitochondria/metabolism ; Protein Interaction Domains and Motifs ; Rats ; Subcellular Fractions ; Synaptosomes/metabolism ; Synaptosomes/ultrastructure
Chemical Substances	DNA, Mitochondrial ; Cytochromes c (9007-43-6)
Language	English
Publishing date	2021-01-06
Publishing country	England
Document type	Journal Article
ISSN	1477-3155
ISSN (online)	1477-3155
DOI	10.1186/s12951-020-00748-6
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Article: Data concerning the rheological behavior of high methoxyl pectin during gelation process

Giacomazza, Daniela / Bulone, Donatella / San Biagio, Pier Luigi / Marino, Rosamaria / Lapasin, Romano

Data in Brief. 2018 June, v. 18

2018

Abstract: The present data concern the structuring kinetics of aqueous high methoxyl pectin (HMP) solutions at acid pH (3.1), constant pectin concentration (0.2% w/w) and sucrose concentrations ranging from 56 to 65% w/w. Consecutive frequency sweep was applied to ...

Abstract	The present data concern the structuring kinetics of aqueous high methoxyl pectin (HMP) solutions at acid pH (3.1), constant pectin concentration (0.2% w/w) and sucrose concentrations ranging from 56 to 65% w/w. Consecutive frequency sweep was applied to samples immediately after their preparation. The generalized Maxwell (gM) model was used to describe the change of the mechanical spectra for each different sucrose concentration and to determine the viscoelastic parameters controlling the gelation of the HMP solutions. The viscosities in the sol region are explored in the range 0 to 55% 0 to 40% (w/w) sucrose concentration.
Keywords	gelation ; models ; pH ; pectins ; sucrose ; viscoelasticity
Language	English
Dates of publication	2018-06
Size	p. 1628-1631.
Publishing place	Elsevier Inc.
Document type	Article
ZDB-ID	2786545-9
ISSN	2352-3409
ISSN	2352-3409
DOI	10.1016/j.dib.2018.04.064
Database	NAL-Catalogue (AGRICOLA)

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Article: Recovery from Food Waste—Biscuit Doughs Enriched with Pomegranate Peel Powder as a Model of Fortified Aliment

Nuzzo, Domenico / Picone, Pasquale / Lozano Sanchez, Jesus / Borras-Linares, Isabel / Guiducci, Alessandro / Muscolino, Emanuela / Giacomazza, Daniela / Sanfilippo, Tiziana / Guggino, Rossella / Bulone, Donatella / Dispenza, Clelia / San Biagio, Pier Luigi / Lapasin, Romano

Biology. 2022 Mar. 09, v. 11, no. 3

2022

Abstract: The aim of the present work is the characterization of biscuit doughs enriched with pomegranate peel powder (PPP) at 3 (PPP3) and 5 (PPP5) wt% in the prospect of developing a fortified aliment as a support of the therapy of chronic inflammatory diseases ... ...

Abstract	The aim of the present work is the characterization of biscuit doughs enriched with pomegranate peel powder (PPP) at 3 (PPP3) and 5 (PPP5) wt% in the prospect of developing a fortified aliment as a support of the therapy of chronic inflammatory diseases of the intestinal tract. The total phenolic content of the powder was preliminarily evaluated. Then, the main compounds present in the PPP were identified by HPLC-ESI-TOF-MS analysis, being mainly hydrolysable tannins. The PPP was then treated at 180 °C for 20 min to mimic the baking treatment, and its water-soluble fraction (PPPwsf) was then added in the Caco-2 cell culture as a model of the intestinal epithelial barrier to verify its dose-dependent toxicity, ability in counteracting the oxidative stress, and anti-inflammatory action. Rheological experiments were performed to predict the macroscopic behavior of the PPP-added doughs during lamination and biscuit baking. SEM investigations gave their contribution to the microscopic comprehension of the dough structure. Finally, a consumer panel composed by thirty volunteers was enrolled to express its opinion on the sensory agreeableness of the biscuits prepared with two different concentrations of PPP compared with the reference dough. The discussion is focused on the biological effects of the main components found in the PPP.
Keywords	anti-inflammatory activity ; cell culture ; dose response ; dough ; epithelium ; human cell lines ; intestines ; models ; oxidative stress ; pomegranates ; therapeutics ; toxicity ; water solubility
Language	English
Dates of publication	2022-0309
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article
ZDB-ID	2661517-4
ISSN	2079-7737
ISSN	2079-7737
DOI	10.3390/biology11030416
Database	NAL-Catalogue (AGRICOLA)

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Article: Recovery from Food Waste-Biscuit Doughs Enriched with Pomegranate Peel Powder as a Model of Fortified Aliment.

Biology

2022 Volume 11, Issue 3

Abstract	The aim of the present work is the characterization of biscuit doughs enriched with pomegranate peel powder (PPP) at 3 (PPP3) and 5 (PPP5) wt% in the prospect of developing a fortified aliment as a support of the therapy of chronic inflammatory diseases of the intestinal tract. The total phenolic content of the powder was preliminarily evaluated. Then, the main compounds present in the PPP were identified by HPLC-ESI-TOF-MS analysis, being mainly hydrolysable tannins. The PPP was then treated at 180 °C for 20 min to mimic the baking treatment, and its water-soluble fraction (PPPwsf) was then added in the Caco-2 cell culture as a model of the intestinal epithelial barrier to verify its dose-dependent toxicity, ability in counteracting the oxidative stress, and anti-inflammatory action. Rheological experiments were performed to predict the macroscopic behavior of the PPP-added doughs during lamination and biscuit baking. SEM investigations gave their contribution to the microscopic comprehension of the dough structure. Finally, a consumer panel composed by thirty volunteers was enrolled to express its opinion on the sensory agreeableness of the biscuits prepared with two different concentrations of PPP compared with the reference dough. The discussion is focused on the biological effects of the main components found in the PPP.
Language	English
Publishing date	2022-03-09
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2661517-4
ISSN	2079-7737
ISSN	2079-7737
DOI	10.3390/biology11030416
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Article ; Online: From Small Peptides to Large Proteins against Alzheimer'sDisease.

Picone, Pasquale / Sanfilippo, Tiziana / Vasto, Sonya / Baldassano, Sara / Guggino, Rossella / Nuzzo, Domenico / Bulone, Donatella / San Biagio, Pier Luigi / Muscolino, Emanuela / Monastero, Roberto / Dispenza, Clelia / Giacomazza, Daniela

Biomolecules

2022 Volume 12, Issue 10

Abstract: Alzheimer's disease (AD) is the most common neurodegenerative disorder in the elderly. The two cardinal neuropathological hallmarks of AD are the senile plaques, which are extracellular deposits mainly constituted by beta-amyloids, and neurofibrillary ... ...

Abstract	Alzheimer's disease (AD) is the most common neurodegenerative disorder in the elderly. The two cardinal neuropathological hallmarks of AD are the senile plaques, which are extracellular deposits mainly constituted by beta-amyloids, and neurofibrillary tangles formed by abnormally phosphorylated Tau (p-Tau) located in the cytoplasm of neurons. Although the research has made relevant progress in the management of the disease, the treatment is still lacking. Only symptomatic medications exist for the disease, and, in the meantime, laboratories worldwide are investigating disease-modifying treatments for AD. In the present review, results centered on the use of peptides of different sizes involved in AD are presented.
MeSH term(s)	Humans ; Aged ; Alzheimer Disease/metabolism ; tau Proteins/metabolism ; Amyloid beta-Peptides/metabolism ; Plaque, Amyloid/metabolism ; Plaque, Amyloid/pathology ; Neurons/metabolism ; Neurofibrillary Tangles/metabolism ; Neurofibrillary Tangles/pathology
Chemical Substances	tau Proteins ; Amyloid beta-Peptides
Language	English
Publishing date	2022-09-22
Publishing country	Switzerland
Document type	Journal Article ; Review ; Research Support, Non-U.S. Gov't
ZDB-ID	2701262-1
ISSN	2218-273X ; 2218-273X
ISSN (online)	2218-273X
ISSN	2218-273X
DOI	10.3390/biom12101344
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Article ; Online: Recombinant mussel protein Pvfp5β enhances cell adhesion of poly(vinyl alcohol)/k-carrageenan hydrogel scaffolds.

Muscolino, Emanuela / Costa, Maria Assunta / Sabatino, Maria Antonietta / Alessi, Sabina / Bulone, Donatella / San Biagio, Pier Luigi / Passantino, Rosa / Giacomazza, Daniela / Dispenza, Clelia

International journal of biological macromolecules

2022 Volume 211, Page(s) 639–652

Abstract	Polymeric hydrogels are increasingly considered as scaffolds for tissue engineering due to their extraordinary resemblance with the extracellular matrix (ECM) of many tissues. As cell adhesion is a key factor in regulating important cell functions, hydrogel scaffolds are often functionalized or loaded with a variety of bioactive molecules that can promote adhesion. Interesting biomimetic approaches exploit the properties of mussel-inspired recombinant adhesive proteins. In this work, we prepared hydrogel scaffolds with a 50%w mixture of k-carrageenan (kC) and polyvinyl alcohol (PVA), by a two-step physical gelation process, and we coated them with Perna viridis foot protein-5β (Pvfp5β). The mechanical and morphological properties of hydrogels were investigated both after conditioning with typical cell culture media and also after coating with the Pvfp5β. The protein resulted strongly adsorbed onto the surface of the hydrogel and also able to penetrate in its interiors to a certain depth, mainly interacting with the kC component of the scaffold as resulted from the confocal analysis. Mouse embryonic fibroblasts NIH-3T3 were seeded on top of the hydrogels and cultured up to two weeks. The role of Pvfp5β in promoting cell adhesion, spreading and colonization of the scaffold was demonstrated.
MeSH term(s)	Animals ; Carrageenan/metabolism ; Cell Adhesion/physiology ; Fibroblasts/metabolism ; Hydrogels/metabolism ; Hydrogels/pharmacology ; Mice ; Polyvinyl Alcohol/metabolism ; Recombinant Proteins/metabolism ; Tissue Engineering/methods ; Tissue Scaffolds
Chemical Substances	Hydrogels ; Recombinant Proteins ; Carrageenan (9000-07-1) ; Polyvinyl Alcohol (9002-89-5)
Language	English
Publishing date	2022-05-13
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	282732-3
ISSN	1879-0003 ; 0141-8130
ISSN (online)	1879-0003
ISSN	0141-8130
DOI	10.1016/j.ijbiomac.2022.05.068
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Data concerning the rheological behavior of high methoxyl pectin during gelation process.

Giacomazza, Daniela / Bulone, Donatella / San Biagio, Pier Luigi / Marino, Rosamaria / Lapasin, Romano

Data in brief

2018 Volume 18, Page(s) 1628–1631

Abstract	The present data concern the structuring kinetics of aqueous high methoxyl pectin (HMP) solutions at acid pH (3.1), constant pectin concentration (0.2% w/w) and sucrose concentrations ranging from 56 to 65% w/w. Consecutive frequency sweep was applied to samples immediately after their preparation. The generalized Maxwell (gM) model was used to describe the change of the mechanical spectra for each different sucrose concentration and to determine the viscoelastic parameters controlling the gelation of the HMP solutions. The viscosities in the sol region are explored in the range 0 to 55% 0 to 40% (w/w) sucrose concentration.
Language	English
Publishing date	2018-04-24
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	2786545-9
ISSN	2352-3409
ISSN	2352-3409
DOI	10.1016/j.dib.2018.04.064
Database	MEDical Literature Analysis and Retrieval System OnLINE

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