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  1. Book: Unser Baby im Ultraschall

    Burger, Michael

    ein Begleiter für werdende Eltern. Mit 101 Abbildungen

    2017  

    Author's details Michael Burger
    Keywords Schwangerenvorsorge ; Ultraschalldiagnostik
    Subject Schwangerschaft ; Schwangerschaftsvorsorge ; Sonographie ; Sonografie ; Ultrasonographie ; Ultrasonografie
    Language German
    Size XII, 170 Seiten, Illustrationen, 24 cm
    Edition 1. Auflage
    Publisher Springer
    Publishing place Berlin
    Publishing country Germany
    Document type Book
    HBZ-ID HT019401283
    ISBN 978-3-662-53457-1 ; 3-662-53457-6 ; 9783662534588 ; 3662534584
    Database Catalogue ZB MED Medicine, Health

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    In stock of ZB MED Cologne/Königswinter

    Shelf markLoan statusLocation
    2010 A 6641 order for loan Magazin

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  2. Book ; Thesis: Expression und Funktion von Homebox- und bHLH-Transkriptionsfaktoren bei malignen Gliomen

    Burger, Michael C.

    2012  

    Author's details vorgelegt von Michael Christian Burger
    Language German
    Size 135 S. : graph. Darst.
    Publishing country Germany
    Document type Book ; Thesis
    Thesis / German Habilitation thesis Tübingen, Univ., Diss., 2012
    HBZ-ID HT017338163
    Database Catalogue ZB MED Medicine, Health

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    Shelf markLoan statusLocation
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  3. Article ; Online: Daten, Künstliche Intelligenz und Maschinelles Lernen in der Fahrzeugentwicklung

    Burger, Michael

    2022  

    Abstract: 179 ... 185 ... Daten, Künstliche Intelligenz und Maschinelles Lernen sind heutzutage als Schlagworte in nahezu aller Munde, so auch in den Bereichen Fahrzeugentwicklung, Verkehr und Mobilität. In diesem Beitrag wird anhand vonausgewählten ... ...

    Abstract 179

    185

    Daten, Künstliche Intelligenz und Maschinelles Lernen sind heutzutage als Schlagworte in nahezu aller Munde, so auch in den Bereichen Fahrzeugentwicklung, Verkehr und Mobilität. In diesem Beitrag wird anhand vonausgewählten Anwendungsbeispielen aufgezeigt, wie man mit vorhandenen Daten, entsprechenden Technikenund mathematischen Werzeugen die Fahrzeugentwicklung unterstützen und verbessern kann.

    30

    3
    Keywords Daten ; Künstliche Intelligenz ; Machinelles Lernen ; DDC::500 Naturwissenschaften und Mathematik ; DDC::000 Informatik ; Informationswissenschaft ; allgemeine Werke
    Language German
    Publishing date 2022-09-30
    Publishing country de
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: The intracellular visualization of exogenous DNA in fluorescence microscopy.

    Greitens, Christina / Leroux, Jean-Christophe / Burger, Michael

    Drug delivery and translational research

    2024  

    Abstract: In the development of non-viral gene delivery vectors, it is essential to reliably localize and quantify transfected DNA inside the cell. To track DNA, fluorescence microscopy methods are commonly applied. These mostly rely on fluorescently labeled DNA, ... ...

    Abstract In the development of non-viral gene delivery vectors, it is essential to reliably localize and quantify transfected DNA inside the cell. To track DNA, fluorescence microscopy methods are commonly applied. These mostly rely on fluorescently labeled DNA, DNA binding proteins fused to a fluorescent protein, or fluorescence in situ hybridization (FISH). In addition, co-stainings are often used to determine the colocalization of the DNA in specific cellular compartments, such as the endolysosomes or the nucleus. We provide an overview of these DNA tracking methods, advice on how they should be combined, and indicate which co-stainings or additional methods are required to draw precise conclusions from a DNA tracking experiment. Some emphasis is given to the localization of exogenous DNA inside the nucleus, which is the last step of DNA delivery. We argue that suitable tools which allow for the nuclear detection of faint signals are still missing, hampering the rational development of more efficient non-viral transfection systems.
    Language English
    Publishing date 2024-03-25
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2590155-2
    ISSN 2190-3948 ; 2190-393X
    ISSN (online) 2190-3948
    ISSN 2190-393X
    DOI 10.1007/s13346-024-01563-4
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Revival of Bioengineered Proteins as Carriers for Nucleic Acids.

    Scherer, David / Burger, Michael / Leroux, Jean-Christophe

    Bioconjugate chemistry

    2024  

    Language English
    Publishing date 2024-04-15
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1024041-x
    ISSN 1520-4812 ; 1043-1802
    ISSN (online) 1520-4812
    ISSN 1043-1802
    DOI 10.1021/acs.bioconjchem.4c00079
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 3400: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  6. Article ; Online: Rezension

    Burger, Michael

    Medienimpulse; Bd. 58 Nr.; 6 Seiten ; Media Impulses; Vol 58 No 02 (2020): 2/2020 – Nähe(n) und Distanz(en) in Zeiten der COVID-19-Krise; 6 Seiten ; 2307-3187

    Wie Stories zu History werden. Zur Authentizität von Zeitgeschichte im Spielfilm von Björn Bergold

    2020  Volume 02, Issue : 2/2020 – Nähe(n) und Distanz(en) in Zeiten der COVID-19-Krise

    Abstract: Björn Bergold geht am Beispiel des Fernsehzweiteilers „Der Turm“, der das Ende der DDR thematisiert, in seiner empirischen Studie der Frage nach, wie und anhand welcher Aspekte fiktionale zeithistorische Spielfilme als eine authentische Darstellung von ... ...

    Abstract Björn Bergold geht am Beispiel des Fernsehzweiteilers „Der Turm“, der das Ende der DDR thematisiert, in seiner empirischen Studie der Frage nach, wie und anhand welcher Aspekte fiktionale zeithistorische Spielfilme als eine authentische Darstellung von Geschichte rezipiert werden.

    Using the example of the two-parted TV-movie „Der Turm“, which thematisies the end of the GDR, Björn Bergold explores in his empirical study, how and on the basis of which aspects ficitonal contemporary historical films are adopted as an authentic depiction of history.
    Keywords covid19
    Language German
    Publishing date 2020-06-21
    Publisher Bundesministerium für Bildung, Wissenschaft und Forschung
    Publishing country at
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  7. Thesis ; Online: Search for Small Molecule Enhancers for DNA Delivery and Development of Recombinant Protein-Based Transfection Agents

    Burger, Michael

    2020  

    Keywords info:eu-repo/classification/ddc/570 ; info:eu-repo/classification/ddc/610 ; Life sciences ; Medical sciences ; medicine
    Language English
    Publisher ETH Zurich
    Publishing country ch
    Document type Thesis ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  8. Article ; Online: Get out or die trying: Peptide- and protein-based endosomal escape of RNA therapeutics.

    Klipp, Alexander / Burger, Michael / Leroux, Jean-Christophe

    Advanced drug delivery reviews

    2023  Volume 200, Page(s) 115047

    Abstract: RNA therapeutics offer great potential to transform the biomedical landscape, encompassing the treatment of hereditary conditions and the development of better vaccines. However, the delivery of RNAs into the cell is hampered, among others, by poor ... ...

    Abstract RNA therapeutics offer great potential to transform the biomedical landscape, encompassing the treatment of hereditary conditions and the development of better vaccines. However, the delivery of RNAs into the cell is hampered, among others, by poor endosomal escape. This major hurdle is often tackled using special lipids, polymers, or protein-based delivery vectors. In this review, we will focus on the most prominent peptide- and protein-based endosomal escape strategies with focus on RNA drugs. We discuss cell penetrating peptides, which are still incorporated into novel transfection systems today to promote endosomal escape. However, direct evidence for enhanced endosomal escape by the action of such peptides is missing and their transfection efficiency, even in permissive cell culture conditions, is rather low. Endosomal escape by the help of pore forming proteins or phospholipases, on the other hand, allowed to generate more efficient transfection systems. These are, however, often hampered by considerable toxicity and immunogenicity. We conclude that the perfect enhancer of endosomal escape has yet to be devised. To increase the chances of success, any new transfection system should be tested under relevant conditions and guided by assays that allow direct quantification of endosomal escape.
    MeSH term(s) Humans ; Proteins/metabolism ; Endosomes/metabolism ; Cell-Penetrating Peptides/metabolism ; Transfection ; RNA, Small Interfering/genetics
    Chemical Substances Proteins ; Cell-Penetrating Peptides ; RNA, Small Interfering
    Language English
    Publishing date 2023-08-02
    Publishing country Netherlands
    Document type Journal Article ; Review ; Research Support, Non-U.S. Gov't
    ZDB-ID 639113-8
    ISSN 1872-8294 ; 0169-409X
    ISSN (online) 1872-8294
    ISSN 0169-409X
    DOI 10.1016/j.addr.2023.115047
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 2241: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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    Jg. 1995 - 2021: Lesesall (1.OG)
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  9. Article ; Online: A dedicated cytoplasmic container collects extrachromosomal DNA away from the mammalian nucleus.

    Schenkel, Laura / Wang, Xuan / Le, Nhung / Burger, Michael / Kroschewski, Ruth

    Molecular biology of the cell

    2023  Volume 34, Issue 11, Page(s) ar105

    Abstract: Expression from transfected plasmid DNA is generally transient, but it is unclear what process terminates it. We show that DNA entering mammalian cells is rapidly surrounded by a double membrane in the cytoplasm, in some cases after leaving the nucleus. ... ...

    Abstract Expression from transfected plasmid DNA is generally transient, but it is unclear what process terminates it. We show that DNA entering mammalian cells is rapidly surrounded by a double membrane in the cytoplasm, in some cases after leaving the nucleus. This cytoplasmic container, termed exclusome, frequently also contains extrachromosomal telomeric DNA, and is maintained by the cell over several division cycles. The exclusome envelope contains endoplasmic reticulum proteins and the inner-nuclear membrane proteins Lap2β and Emerin, but differs from the nuclear envelope by its fenestrations and the absence of the Lamin B Receptor and nuclear pore complexes. Reduction of exclusome frequency upon overexpressing Emerin's LEM-domain suggests a role for Emerin in plasmid DNA compartmentalization. Thus, cells distinguish extrachromosomal DNA and chromosomes and wrap them into similar yet distinct envelopes keeping the former in the exclusome but the latter in the nucleus, where transcription occurs.
    MeSH term(s) Animals ; Nuclear Envelope/metabolism ; Cell Nucleus/metabolism ; Nuclear Pore ; Cytoplasm ; Chromosomes ; Mammals
    Language English
    Publishing date 2023-08-09
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1098979-1
    ISSN 1939-4586 ; 1059-1524
    ISSN (online) 1939-4586
    ISSN 1059-1524
    DOI 10.1091/mbc.E23-04-0118
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 2853: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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    Zs.MO 410: Show issues

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  10. Article ; Online: The TFAMoplex-Conversion of the Mitochondrial Transcription Factor A into a DNA Transfection Agent.

    Burger, Michael / Kaelin, Seraina / Leroux, Jean-Christophe

    Advanced science (Weinheim, Baden-Wurttemberg, Germany)

    2022  Volume 9, Issue 8, Page(s) e2104987

    Abstract: Non-viral gene delivery agents, such as cationic lipids, polymers, and peptides, mainly rely on charge-based and hydrophobic interactions for the condensation of DNA molecules into nanoparticles. The human protein mitochondrial transcription factor A ( ... ...

    Abstract Non-viral gene delivery agents, such as cationic lipids, polymers, and peptides, mainly rely on charge-based and hydrophobic interactions for the condensation of DNA molecules into nanoparticles. The human protein mitochondrial transcription factor A (TFAM), on the other hand, has evolved to form nanoparticles with DNA through highly specific protein-protein and protein-DNA interactions. Here, the properties of TFAM are repurposed to create a DNA transfection agent by means of protein engineering. TFAM is covalently fused to Listeria monocytogenes phospholipase C (PLC), an enzyme that lyses lipid membranes under acidic conditions, to enable endosomal escape and human vaccinia-related kinase 1 (VRK1), which is intended to protect the DNA from cytoplasmic defense mechanisms. The TFAM/DNA complexes (TFAMoplexes) are stabilized by cysteine point mutations introduced rationally in the TFAM homodimerization site, resulting in particles, which show maximal activity when formed in 80% serum and transfect HeLa cells in vitro after 30 min of incubation under challenging cell culture conditions. The herein developed TFAM-based DNA scaffolds combine interesting characteristics in an easy-to-use system and can be readily expanded with further protein factors. This makes the TFAMoplex a promising tool in protein-based gene delivery.
    MeSH term(s) DNA/genetics ; DNA/metabolism ; DNA-Binding Proteins/genetics ; DNA-Binding Proteins/metabolism ; HeLa Cells ; Humans ; Intracellular Signaling Peptides and Proteins/genetics ; Mitochondrial Proteins ; Protein Serine-Threonine Kinases ; Transcription Factors ; Transfection
    Chemical Substances DNA-Binding Proteins ; Intracellular Signaling Peptides and Proteins ; Mitochondrial Proteins ; Transcription Factors ; mitochondrial transcription factor A ; DNA (9007-49-2) ; Protein Serine-Threonine Kinases (EC 2.7.11.1) ; VRK1 protein, human (EC 2.7.11.1)
    Language English
    Publishing date 2022-01-17
    Publishing country Germany
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2808093-2
    ISSN 2198-3844 ; 2198-3844
    ISSN (online) 2198-3844
    ISSN 2198-3844
    DOI 10.1002/advs.202104987
    Database MEDical Literature Analysis and Retrieval System OnLINE

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