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  1. AU="Burtnick, Mary N"
  2. AU=Frisoni Giovanni B
  3. AU="Dhiraj Kumar"
  4. AU="Isaac S. Lee"
  5. AU="Bals, Julia"
  6. AU="Rovira-Clavé, Xavier"
  7. AU="Den Boer, Monique L"
  8. AU="Potts, T."
  9. AU="Cifuentes-Diaz, Carmen"
  10. AU="Alvim, Ricardo G"
  11. AU="Barron II, Joseph C"
  12. AU="Godin, Shea-Lee"
  13. AU="Leng, Chengcai"
  14. AU="Hyslop, Brian W"
  15. AU="Suzanne Fischer"
  16. AU="Aboelata, Noha"
  17. AU="Chiang, Sarah N"
  18. AU="Wessel, Kristin M"
  19. AU="Wilson, Jenna M"
  20. AU="Goines, Paula"
  21. AU=Ippolito Mariachiara AU=Ippolito Mariachiara
  22. AU="Jose Chauca"
  23. AU="Asih, Puji B S"
  24. AU="Dsane-Selby, Lydia"
  25. AU="Tolossa, Tadesse"
  26. AU="Erdal Bedir"

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  1. Artikel ; Online: Use of Reductive Amination to Produce Capsular Polysaccharide-Based Glycoconjugates.

    Urbano-Munoz, Federico / Orne, Caitlyn E / Burtnick, Mary N / Brett, Paul J

    Methods in molecular biology (Clifton, N.J.)

    2024  Band 2762, Seite(n) 139–148

    Abstract: Reductive amination is a relatively simple and convenient strategy for coupling purified polysaccharides to carrier proteins. Following their synthesis, glycoconjugates can be used to assess the protective capacity of specific microbial polysaccharides ... ...

    Abstract Reductive amination is a relatively simple and convenient strategy for coupling purified polysaccharides to carrier proteins. Following their synthesis, glycoconjugates can be used to assess the protective capacity of specific microbial polysaccharides in animal models of infection and/or to produce polyclonal antiserum and monoclonal antibodies for a variety of immune assays. Here, we describe a reproducible method for chemically activating the 6-deoxyheptan capsular polysaccharide (CPS) from Burkholderia pseudomallei and covalently linking it to recombinant CRM197 diphtheria toxin mutant (CRM197) to produce the glycoconjugate, CPS-CRM197. Similar approaches can also be used to couple other types of polysaccharides to CRM197 with little to no modification of the protocol.
    Mesh-Begriff(e) Animals ; Amination ; Polysaccharides ; Burkholderia pseudomallei ; Glycoconjugates ; Vaccines, Conjugate
    Chemische Substanzen Polysaccharides ; Glycoconjugates ; Vaccines, Conjugate
    Sprache Englisch
    Erscheinungsdatum 2024-02-05
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-3666-4_9
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  2. Artikel ; Online: Functional Activities of O-Polysaccharide and Hemolysin Coregulated Protein 1 Specific Antibodies Isolated from Melioidosis Patients.

    Pumpuang, Apinya / Paksanont, Suporn / Burtnick, Mary N / Brett, Paul J / Chantratita, Narisara

    Infection and immunity

    2022  Band 90, Heft 11, Seite(n) e0021422

    Abstract: Melioidosis is a fatal tropical disease caused by the environmental Gram-negative bacterium, Burkholderia pseudomallei. This bacterium is intrinsically resistant to several antibiotics and treatment of melioidosis requires prolonged antibiotic ... ...

    Abstract Melioidosis is a fatal tropical disease caused by the environmental Gram-negative bacterium, Burkholderia pseudomallei. This bacterium is intrinsically resistant to several antibiotics and treatment of melioidosis requires prolonged antibiotic administration. To date, there are no vaccines available for melioidosis. Previous studies have shown that humoral immunity is critical for surviving melioidosis and that O-polysaccharide (OPS) and hemolysin coregulated protein 1 (Hcp1) are important protective antigens in animal models of melioidosis. Our previous studies revealed that melioidosis patients had high levels of OPS- and Hcp1-specific antibodies and that IgG against OPS (IgG-OPS) and Hcp1 (IgG-Hcp1) were associated with patient survival. In this study, we characterized the potential function(s) of IgG-OPS and IgG-Hcp1 from melioidosis patients. IgG-OPS and IgG-Hcp1 were purified from pooled serum obtained from melioidosis patients using immuno-affinity chromatography. Antibody-dependent cellular phagocytosis assays were performed with pooled serum from melioidosis patients and compared with serum obtained from healthy controls. Serum from melioidosis patients significantly enhanced B. pseudomallei uptake into the human monocytic cell line THP-1 compared with pooled serum from healthy donors. Enhanced opsonization was observed with IgG-OPS and IgG-Hcp1 in a dose-dependent manner. Antibody-dependent complement deposition assays were performed with IgG-OPS and IgG-Hcp1 using flow cytometry and showed that there was enhanced C3b deposition on the surface of B. pseudomallei treated with IgG-OPS but to a lesser degree with IgG-Hcp1. This study provides insight into the function of IgG-OPS and IgG-Hcp1 in human melioidosis and supports that OPS and Hcp1 are potential vaccine antigens for immunization against melioidosis.
    Mesh-Begriff(e) Humans ; Antibodies, Bacterial ; Burkholderia pseudomallei ; Hemolysin Proteins ; Immunoglobulin G ; Melioidosis ; Polysaccharides
    Chemische Substanzen Antibodies, Bacterial ; Hemolysin Proteins ; Immunoglobulin G ; Polysaccharides
    Sprache Englisch
    Erscheinungsdatum 2022-10-13
    Erscheinungsland United States
    Dokumenttyp Journal Article ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, Non-U.S. Gov't
    ZDB-ID 218698-6
    ISSN 1098-5522 ; 0019-9567
    ISSN (online) 1098-5522
    ISSN 0019-9567
    DOI 10.1128/iai.00214-22
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  3. Artikel ; Online: Development of Melioidosis Subunit Vaccines Using an Enzymatically Inactive Burkholderia pseudomallei AhpC.

    Schmidt, Lindsey K / Orne, Caitlyn E / Shaffer, Teresa L / Wilson, Shane M / Khakhum, Nittaya / Torres, Alfredo G / Brett, Paul J / Burtnick, Mary N

    Infection and immunity

    2022  Band 90, Heft 8, Seite(n) e0022222

    Abstract: Burkholderia pseudomallei, the causative agent of melioidosis, is a facultative intracellular, Gram-negative pathogen that is highly infectious via the respiratory route and can cause severe, debilitating, and often fatal diseases in humans and animals. ... ...

    Abstract Burkholderia pseudomallei, the causative agent of melioidosis, is a facultative intracellular, Gram-negative pathogen that is highly infectious via the respiratory route and can cause severe, debilitating, and often fatal diseases in humans and animals. At present, no licensed vaccines for immunization against this CDC Tier 1 select agent exist. Studies in our lab have previously demonstrated that subunit vaccine formulations consisting of a B. pseudomallei capsular polysaccharide (CPS)-based glycoconjugate (CPS-CRM197) combined with hemolysin-coregulated protein (Hcp1) provided C57BL/6 mice with high-level protection against an acute inhalational challenge of B. pseudomallei. In this study, we evaluated the immunogenicity and protective capacity of B. pseudomallei alkyl hydroperoxide reductase subunit C (AhpC) in combination with CPS-CRM197. AhpC is a peroxiredoxin involved in oxidative stress reduction and is a potential protective antigen. To facilitate our studies and maximize safety in animals, recombinant B. pseudomallei AhpC harboring an active site mutation (AhpC
    Mesh-Begriff(e) Animals ; Antibodies, Bacterial ; Bacterial Vaccines ; Burkholderia pseudomallei/genetics ; Glycoconjugates ; Humans ; Immunoglobulin G ; Melioidosis/prevention & control ; Mice ; Mice, Inbred BALB C ; Mice, Inbred C57BL ; Vaccines, Subunit/genetics
    Chemische Substanzen Antibodies, Bacterial ; Bacterial Vaccines ; Glycoconjugates ; Immunoglobulin G ; Vaccines, Subunit
    Sprache Englisch
    Erscheinungsdatum 2022-07-11
    Erscheinungsland United States
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 218698-6
    ISSN 1098-5522 ; 0019-9567
    ISSN (online) 1098-5522
    ISSN 0019-9567
    DOI 10.1128/iai.00222-22
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  4. Artikel ; Online: Identification of

    Burtnick, Mary N / Dance, David A B / Vongsouvath, Manivanh / Newton, Paul N / Dittrich, Sabine / Sendouangphachanh, Amphone / Woods, Kate / Davong, Viengmon / Kenna, Dervla T D / Saiprom, Natnaree / Sengyee, Sineenart / Hantrakun, Viriya / Wuthiekanun, Vanaporn / Limmathurotsakul, Direk / Chantratita, Narisara / Brett, Paul J

    Microbiology spectrum

    2024  Band 12, Heft 3, Seite(n) e0332123

    Abstract: Burkholderia ... ...

    Abstract Burkholderia pseudomallei
    Mesh-Begriff(e) Animals ; Humans ; Burkholderia pseudomallei/genetics ; Melioidosis/diagnosis ; Melioidosis/microbiology ; Burkholderia cepacia/genetics ; Polysaccharides ; Antibodies, Monoclonal ; Soil
    Chemische Substanzen Polysaccharides ; Antibodies, Monoclonal ; Soil
    Sprache Englisch
    Erscheinungsdatum 2024-02-01
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ZDB-ID 2807133-5
    ISSN 2165-0497 ; 2165-0497
    ISSN (online) 2165-0497
    ISSN 2165-0497
    DOI 10.1128/spectrum.03321-23
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  5. Artikel ; Online: Prospective Analysis of Antibody Diagnostic Tests and TTS1 Real-Time PCR for Diagnosis of Melioidosis in Areas Where It Is Endemic.

    Noparatvarakorn, Chawitar / Sengyee, Sineenart / Yarasai, Atchara / Phunpang, Rungnapa / Dulsuk, Adul / Ottiwet, Orawan / Janon, Rachan / Morakot, Chumpol / Burtnick, Mary N / Brett, Paul J / West, T Eoin / Chantratita, Narisara

    Journal of clinical microbiology

    2023  Band 61, Heft 3, Seite(n) e0160522

    Abstract: Melioidosis is a tropical infectious disease caused by Burkholderia pseudomallei. Melioidosis is associated with diverse clinical manifestations and high mortality. Early diagnosis is needed for appropriate treatment, but it takes several days to obtain ... ...

    Abstract Melioidosis is a tropical infectious disease caused by Burkholderia pseudomallei. Melioidosis is associated with diverse clinical manifestations and high mortality. Early diagnosis is needed for appropriate treatment, but it takes several days to obtain bacterial culture results. We previously developed a rapid immunochromatography test (ICT) based on hemolysin coregulated protein 1 (Hcp1) and two enzyme-linked immunosorbent assays (ELISAs) based on Hcp1 (Hcp1-ELISA) and O-polysaccharide (OPS-ELISA) for serodiagnosis of melioidosis. This study prospectively validated the diagnostic accuracy of the Hcp1-ICT in suspected melioidosis cases and determined its potential use for identifying occult melioidosis cases. Patients were enrolled and grouped by culture results, including 55 melioidosis cases, 49 other infection patients, and 69 patients with no pathogen detected. The results of the Hcp1-ICT were compared with culture, a real-time PCR test based on type 3 secretion system 1 genes (TTS1-PCR), and ELISAs. Patients in the no-pathogen-detected group were followed for subsequent culture results. Using bacterial culture as a gold standard, the sensitivity and specificity of Hcp1-ICT were 74.5% and 89.8%, respectively. The sensitivity and specificity of TTS1-PCR were 78.2% and 100%, respectively. The diagnostic accuracy was markedly improved if the Hcp1-ICT results were combined with TTS1-PCR results (sensitivity and specificity were 98.2% and 89.8%, respectively). Among patients with initially negative cultures, Hcp1-ICT was positive in 16/73 (21.9%). Five of the 16 patients (31.3%) were subsequently confirmed to have melioidosis by repeat culture. The combined Hcp1-ICT and TTS1-PCR test results are useful for diagnosis, and Hcp1-ICT may help identify occult cases of melioidosis.
    Mesh-Begriff(e) Humans ; Melioidosis/diagnosis ; Melioidosis/microbiology ; Real-Time Polymerase Chain Reaction ; Antibodies, Bacterial ; Burkholderia pseudomallei/genetics ; Sensitivity and Specificity ; Hemolysin Proteins/genetics ; Diagnostic Tests, Routine
    Chemische Substanzen Antibodies, Bacterial ; Hemolysin Proteins
    Sprache Englisch
    Erscheinungsdatum 2023-03-06
    Erscheinungsland United States
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 390499-4
    ISSN 1098-660X ; 0095-1137
    ISSN (online) 1098-660X
    ISSN 0095-1137
    DOI 10.1128/jcm.01605-22
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  6. Artikel ; Online: Burkholderia mallei and Burkholderia pseudomallei cluster 1 type VI secretion system gene expression is negatively regulated by iron and zinc.

    Burtnick, Mary N / Brett, Paul J

    PloS one

    2013  Band 8, Heft 10, Seite(n) e76767

    Abstract: Burkholderia mallei is a facultative intracellular pathogen that causes glanders in humans and animals. Previous studies have demonstrated that the cluster 1 type VI secretion system (T6SS-1) expressed by this organism is essential for virulence in ... ...

    Abstract Burkholderia mallei is a facultative intracellular pathogen that causes glanders in humans and animals. Previous studies have demonstrated that the cluster 1 type VI secretion system (T6SS-1) expressed by this organism is essential for virulence in hamsters and is positively regulated by the VirAG two-component system. Recently, we have shown that T6SS-1 gene expression is up-regulated following internalization of this pathogen into phagocytic cells and that this system promotes multinucleated giant cell formation in infected tissue culture monolayers. In the present study, we further investigated the complex regulation of this important virulence factor. To assess T6SS-1 expression, B. mallei strains were cultured in various media conditions and Hcp1 production was analyzed by Western immunoblotting. Transcript levels of several VirAG-regulated genes (bimA, tssA, hcp1 and tssM) were also determined using quantitative real time PCR. Consistent with previous observations, T6SS-1 was not expressed during growth of B. mallei in rich media. Curiously, growth of the organism in minimal media (M9G) or minimal media plus casamino acids (M9CG) facilitated robust expression of T6SS-1 genes whereas growth in minimal media plus tryptone (M9TG) did not. Investigation of this phenomenon confirmed a regulatory role for VirAG in this process. Additionally, T6SS-1 gene expression was significantly down-regulated by the addition of iron and zinc to M9CG. Other genes under the control of VirAG did not appear to be as tightly regulated by these divalent metals. Similar results were observed for B. pseudomallei, but not for B. thailandensis. Collectively, our findings indicate that in addition to being positively regulated by VirAG, B. mallei and B. pseudomallei T6SS-1 gene expression is negatively regulated by iron and zinc.
    Mesh-Begriff(e) Animals ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Bacterial Secretion Systems/drug effects ; Bacterial Secretion Systems/genetics ; Burkholderia mallei/drug effects ; Burkholderia mallei/genetics ; Burkholderia mallei/growth & development ; Burkholderia pseudomallei/drug effects ; Burkholderia pseudomallei/genetics ; Burkholderia pseudomallei/growth & development ; Cations, Divalent/pharmacology ; Culture Media/pharmacology ; Gene Expression Regulation, Bacterial/drug effects ; Iron/pharmacology ; Multigene Family ; Physical Chromosome Mapping ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; Zinc/pharmacology
    Chemische Substanzen Bacterial Proteins ; Bacterial Secretion Systems ; Cations, Divalent ; Culture Media ; RNA, Messenger ; Iron (E1UOL152H7) ; Zinc (J41CSQ7QDS)
    Sprache Englisch
    Erscheinungsdatum 2013-10-11
    Erscheinungsland United States
    Dokumenttyp Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ISSN 1932-6203
    ISSN (online) 1932-6203
    DOI 10.1371/journal.pone.0076767
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  7. Artikel ; Online: Burkholderia pseudomallei and Burkholderia mallei vaccines: Are we close to clinical trials?

    Titball, Richard W / Burtnick, Mary N / Bancroft, Gregory J / Brett, Paul

    Vaccine

    2017  Band 35, Heft 44, Seite(n) 5981–5989

    Abstract: B. pseudomallei is the cause of melioidosis, a serious an often fatal disease of humans and animals. The closely related bacterium B. mallei, which cases glanders, is considered to be a clonal derivative of B. pseudomallei. Both B. pseudomallei and B. ... ...

    Abstract B. pseudomallei is the cause of melioidosis, a serious an often fatal disease of humans and animals. The closely related bacterium B. mallei, which cases glanders, is considered to be a clonal derivative of B. pseudomallei. Both B. pseudomallei and B. mallei were evaluated by the United States and the former USSR as potential bioweapons. Much of the effort to devise biodefence vaccines in the past decade has been directed towards the identification and formulation of sub-unit vaccines which could protect against both melioidosis and glanders. A wide range of proteins and polysaccharides have been identified which protective immunity in mice. In this review we highlight the significant progress that has been made in developing glycoconjugates as sub-unit vaccines. We also consider some of the important the criteria for licensing, including the suitability of the "animal rule" for assessing vaccine efficacy, the protection required from a vaccine and the how correlates of protection will be identified. Vaccines developed for biodefence purposes could also be used in regions of the world where naturally occurring disease is endemic.
    Sprache Englisch
    Erscheinungsdatum 2017-10-20
    Erscheinungsland Netherlands
    Dokumenttyp Journal Article ; Review
    ZDB-ID 605674-x
    ISSN 1873-2518 ; 0264-410X
    ISSN (online) 1873-2518
    ISSN 0264-410X
    DOI 10.1016/j.vaccine.2017.03.022
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  8. Artikel ; Online: Proteomic analysis of the Burkholderia pseudomallei type II secretome reveals hydrolytic enzymes, novel proteins, and the deubiquitinase TssM.

    Burtnick, Mary N / Brett, Paul J / DeShazer, David

    Infection and immunity

    2014  Band 82, Heft 8, Seite(n) 3214–3226

    Abstract: Burkholderia pseudomallei, the etiologic agent of melioidosis, is an opportunistic pathogen that harbors a wide array of secretion systems, including a type II secretion system (T2SS), three type III secretion systems (T3SS), and six type VI secretion ... ...

    Abstract Burkholderia pseudomallei, the etiologic agent of melioidosis, is an opportunistic pathogen that harbors a wide array of secretion systems, including a type II secretion system (T2SS), three type III secretion systems (T3SS), and six type VI secretion systems (T6SS). The proteins exported by these systems provide B. pseudomallei with a growth advantage in vitro and in vivo, but relatively little is known about the full repertoire of exoproducts associated with each system. In this study, we constructed deletion mutations in gspD and gspE, T2SS genes encoding an outer membrane secretin and a cytoplasmic ATPase, respectively. The secretion profiles of B. pseudomallei MSHR668 and its T2SS mutants were noticeably different when analyzed by SDS-PAGE. We utilized liquid chromatography-tandem mass spectrometry (LC-MS/MS) to identify proteins present in the supernatants of B. pseudomallei MSHR668 and B. pseudomallei ΔgspD grown in rich and minimal media. The MSHR668 supernatants contained 48 proteins that were either absent or substantially reduced in the supernatants of ΔgspD strains. Many of these proteins were putative hydrolytic enzymes, including 12 proteases, two phospholipases, and a chitinase. Biochemical assays validated the LC-MS/MS results and demonstrated that the export of protease, phospholipase C, and chitinase activities is T2SS dependent. Previous studies had failed to identify the mechanism of secretion of TssM, a deubiquitinase that plays an integral role in regulating the innate immune response. Here we present evidence that TssM harbors an atypical signal sequence and that its secretion is mediated by the T2SS. This study provides the first in-depth characterization of the B. pseudomallei T2SS secretome.
    Mesh-Begriff(e) Bacterial Proteins/analysis ; Bacterial Proteins/metabolism ; Bacterial Secretion Systems ; Burkholderia pseudomallei/chemistry ; Burkholderia pseudomallei/genetics ; Burkholderia pseudomallei/metabolism ; Chromatography, Liquid ; Electrophoresis, Polyacrylamide Gel ; Gene Deletion ; Proteome/analysis ; Tandem Mass Spectrometry
    Chemische Substanzen Bacterial Proteins ; Bacterial Secretion Systems ; Proteome
    Sprache Englisch
    Erscheinungsdatum 2014-05-27
    Erscheinungsland United States
    Dokumenttyp Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 218698-6
    ISSN 1098-5522 ; 0019-9567
    ISSN (online) 1098-5522
    ISSN 0019-9567
    DOI 10.1128/IAI.01739-14
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  9. Artikel ; Online: Evaluation of antigen-detecting and antibody-detecting diagnostic test combinations for diagnosing melioidosis.

    Amornchai, Premjit / Hantrakun, Viriya / Wongsuvan, Gumphol / Wuthiekanun, Vanaporn / Wongratanacheewin, Surasakdi / Teparrakkul, Prapit / West, T Eoin / AuCoin, David P / Day, Nicholas P J / Brett, Paul J / Burtnick, Mary N / Chantratitra, Narisara / Limmathurotsakul, Direk

    PLoS neglected tropical diseases

    2021  Band 15, Heft 11, Seite(n) e0009840

    Abstract: Background: Melioidosis, an infectious disease caused by Burkholderia pseudomallei, is endemic in many tropical developing countries and has a high mortality. Here we evaluated combinations of a lateral flow immunoassay (LFI) detecting B. pseudomallei ... ...

    Abstract Background: Melioidosis, an infectious disease caused by Burkholderia pseudomallei, is endemic in many tropical developing countries and has a high mortality. Here we evaluated combinations of a lateral flow immunoassay (LFI) detecting B. pseudomallei capsular polysaccharide (CPS) and enzyme-linked immunosorbent assays (ELISA) detecting antibodies against hemolysin co-regulated protein (Hcp1) or O-polysaccharide (OPS) for diagnosing melioidosis.
    Methodology/principal findings: We conducted a cohort-based case-control study. Both cases and controls were derived from a prospective observational study of patients presenting with community-acquired infections and sepsis in northeast Thailand (Ubon-sepsis). Cases included 192 patients with a clinical specimen culture positive for B. pseudomallei. Controls included 502 patients who were blood culture positive for Staphylococcus aureus, Escherichia coli or Klebsiella pneumoniae or were polymerase chain reaction assay positive for malaria or dengue. Serum samples collected within 24 hours of admission were stored and tested using a CPS-LFI, Hcp1-ELISA and OPS-ELISA. When assessing diagnostic tests in combination, results were considered positive if either test was positive. We selected ELISA cut-offs corresponding to a specificity of 95%. Using a positive cut-off OD of 2.912 for Hcp1-ELISA, the combination of the CPS-LFI and Hcp1-ELISA had a sensitivity of 67.7% (130/192 case patients) and a specificity of 95.0% (477/502 control patients). The sensitivity of the combination (67.7%) was higher than that of the CPS-LFI alone (31.3%, p<0.001) and that of Hcp1-ELISA alone (53.6%, p<0.001). A similar phenomenon was also observed for the combination of CPS-LFI and OPS-ELISA. In case patients, positivity of the CPS-LFI was associated with a short duration of symptoms, high modified Sequential (sepsis-related) Organ Failure Assessment (SOFA) score, bacteraemia and mortality outcome, while positivity of Hcp1-ELISA was associated with a longer duration of symptoms, low modified SOFA score, non-bacteraemia and survival outcome.
    Conclusions/significance: A combination of antigen-antibody diagnostic tests increased the sensitivity of melioidosis diagnosis over individual tests while preserving high specificity. Point-of-care tests for melioidosis based on the use of combination assays should be further developed and evaluated.
    Mesh-Begriff(e) Antibodies, Bacterial/analysis ; Antibodies, Bacterial/immunology ; Antigens, Bacterial/analysis ; Antigens, Bacterial/immunology ; Burkholderia pseudomallei/immunology ; Burkholderia pseudomallei/isolation & purification ; Case-Control Studies ; Diagnostic Tests, Routine/methods ; Enzyme-Linked Immunosorbent Assay/methods ; Humans ; Melioidosis/diagnosis ; Melioidosis/microbiology ; Prospective Studies
    Chemische Substanzen Antibodies, Bacterial ; Antigens, Bacterial
    Sprache Englisch
    Erscheinungsdatum 2021-11-02
    Erscheinungsland United States
    Dokumenttyp Evaluation Study ; Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 2429704-5
    ISSN 1935-2735 ; 1935-2735
    ISSN (online) 1935-2735
    ISSN 1935-2735
    DOI 10.1371/journal.pntd.0009840
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  10. Artikel: Layered and integrated medical countermeasures against

    Klimko, Christopher P / Shoe, Jennifer L / Rill, Nathaniel O / Hunter, Melissa / Dankmeyer, Jennifer L / Talyansky, Yuli / Schmidt, Lindsey K / Orne, Caitlyn E / Fetterer, David P / Biryukov, Sergei S / Burtnick, Mary N / Brett, Paul J / DeShazer, David / Cote, Christopher K

    Frontiers in microbiology

    2022  Band 13, Seite(n) 965572

    Abstract: Burkholderia ... ...

    Abstract Burkholderia pseudomallei
    Sprache Englisch
    Erscheinungsdatum 2022-08-17
    Erscheinungsland Switzerland
    Dokumenttyp Journal Article
    ZDB-ID 2587354-4
    ISSN 1664-302X
    ISSN 1664-302X
    DOI 10.3389/fmicb.2022.965572
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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