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  1. Article: A novel laccase from Trametes polyzona with high performance in the decolorization of textile dyes.

    Bucchieri, Daniela / Mangiagalli, Marco / Martani, Francesca / Butti, Pietro / Lotti, Marina / Serra, Immacolata / Branduardi, Paola

    AMB Express

    2024  Volume 14, Issue 1, Page(s) 32

    Abstract: Laccases are multicopper oxidases able to oxidize several phenolic compounds and find application in numerous industrial applications. Among laccase producers, white-rot fungi represent a valuable source of multiple isoforms and isoenzymes of these ... ...

    Abstract Laccases are multicopper oxidases able to oxidize several phenolic compounds and find application in numerous industrial applications. Among laccase producers, white-rot fungi represent a valuable source of multiple isoforms and isoenzymes of these multicopper oxidases. Here we describe the identification, biochemical characterization, and application of laccase 2 from Trametes polyzona (TP-Lac2), a basidiomycete fungus emerged among others that have been screened by plate assay. This enzyme has an optimal temperature of 50 °C and in acidic conditions it is able to oxidize both phenolic and non-phenolic compounds. The ability of TP-Lac2 to decolorize textile dyes was tested in the presence of natural and synthetic mediators at 30 °C and 50 °C. Our results indicate that TP-Lac2 most efficiently decolorizes (decolorization rate > 75%) malachite green oxalate, orange G, amido black10B and bromocresol purple in the presence of acetosyringone and 2,2'-azinobis (3-ethylbenzthiazoline-6-sulfonate)-ABTS. Overall, the laccase mediator system consisting of TP-Lac2 and the natural mediator acetosyringone has potential as an environmentally friendly alternative for wastewater treatment in the textile industry.
    Language English
    Publishing date 2024-03-20
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2621432-5
    ISSN 2191-0855
    ISSN 2191-0855
    DOI 10.1186/s13568-024-01687-3
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  2. Article ; Online: Valorisation of cotton post-industrial textile waste into lactic acid: chemo-mechanical pretreatment, separate hydrolysis and fermentation using engineered yeast.

    Simonetti, Marta / Butti, Pietro / Di Lorenzo, Raffaella Desiré / Mapelli, Valeria / Branduardi, Paola

    Microbial cell factories

    2024  Volume 23, Issue 1, Page(s) 106

    Abstract: Background: The textile industry has several negative impacts, mainly because it is based on a linear business model that depletes natural resources and produces excessive amounts of waste. Globally, about 75% of textile waste is disposed of in ... ...

    Abstract Background: The textile industry has several negative impacts, mainly because it is based on a linear business model that depletes natural resources and produces excessive amounts of waste. Globally, about 75% of textile waste is disposed of in landfills and only 25% is reused or recycled, while less than 1% is recycled back into new garments. In this study, we explored the valorisation of cotton fabric waste from an apparel textile manufacturing company as valuable biomass to produce lactic acid, a versatile chemical building block.
    Results: Post-industrial cotton patches were pre-treated with the aim of developing a methodology applicable to the industrial site involved. First, a mechanical shredding machine reduced the fabric into individual fibres of maximum 35 mm in length. Afterwards, an alkaline treatment was performed, using NaOH at different concentrations, including a 16% (w/v) NaOH enriched waste stream from the mercerisation of cotton fabrics. The combination of chemo-mechanical pre-treatment and enzymatic hydrolysis led to the maximum recovery yield of 90.46 ± 3.46%, corresponding to 74.96 ± 2.76 g/L of glucose released, which represents a novel valorisation of two different side products (NaOH enriched wastewater and cotton textile waste) of the textile industry. The Saccharomyces cerevisiae strain CEN.PK m850, engineered for redirecting the natural alcoholic fermentation towards a homolactic fermentation, was then used to valorise the glucose-enriched hydrolysate into lactic acid. Overall, the process produced 53.04 g/L ± 0.34 of L-lactic acid, with a yield of 82.7%, being the first example of second-generation biomass valorised with this yeast strain, to the best of our knowledge. Remarkably, the fermentation performances were comparable with the ones obtained in the control medium.
    Conclusion: This study validates the exploitation of cotton post-industrial waste as a possible feedstock for the production of commodity chemicals in microbial cell-based biorefineries. The presented strategy demonstrates the possibility of implementing a circular bioeconomy approach in manufacturing textile industries.
    MeSH term(s) Fermentation ; Industrial Waste ; Saccharomyces cerevisiae ; Lactic Acid ; Hydrolysis ; Sodium Hydroxide ; Textiles ; Glucose
    Chemical Substances Industrial Waste ; Lactic Acid (33X04XA5AT) ; Sodium Hydroxide (55X04QC32I) ; Glucose (IY9XDZ35W2)
    Language English
    Publishing date 2024-04-10
    Publishing country England
    Document type Journal Article
    ZDB-ID 2091377-1
    ISSN 1475-2859 ; 1475-2859
    ISSN (online) 1475-2859
    ISSN 1475-2859
    DOI 10.1186/s12934-024-02384-3
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  3. Article ; Online: pCEC-red: a new vector for easier and faster CRISPR-Cas9 genome editing in Saccharomyces cerevisiae.

    Maestroni, Letizia / Butti, Pietro / Senatore, Vittorio Giorgio / Branduardi, Paola

    FEMS yeast research

    2023  Volume 23

    Abstract: CRISPR-Cas9 technology is widely used for precise and specific editing of Saccharomyces cerevisiae genome to obtain marker-free engineered hosts. Targeted double-strand breaks are controlled by a guide RNA (gRNA), a chimeric RNA containing a structural ... ...

    Abstract CRISPR-Cas9 technology is widely used for precise and specific editing of Saccharomyces cerevisiae genome to obtain marker-free engineered hosts. Targeted double-strand breaks are controlled by a guide RNA (gRNA), a chimeric RNA containing a structural segment for Cas9 binding and a 20-mer guide sequence that hybridises to the genomic DNA target. Introducing the 20-mer guide sequence into gRNA expression vectors often requires complex, time-consuming, and/or expensive cloning procedures. We present a new plasmid for CRISPR-Cas9 genome editing in S. cerevisiae, pCEC-red. This tool allows to (i) transform yeast with both Cas9 and gRNA expression cassettes in a single plasmid and (ii) insert the 20-mer sequence in the plasmid with high efficiency, thanks to Golden Gate Assembly and (iii) a red chromoprotein-based screening to speed up the selection of correct plasmids. We tested genome-editing efficiency of pCEC-red by targeting the ADE2 gene. We chose three different 20-mer targets and designed two types of repair fragments to test pCEC-red for precision editing and for large DNA region replacement procedures. We obtained high efficiencies (∼90%) for both engineering procedures, suggesting that the pCEC system can be used for fast and reliable marker-free genome editing.
    MeSH term(s) Gene Editing/methods ; Saccharomyces cerevisiae/genetics ; CRISPR-Cas Systems ; Plasmids ; DNA/metabolism ; RNA, Guide, CRISPR-Cas Systems
    Chemical Substances DNA (9007-49-2) ; RNA, Guide, CRISPR-Cas Systems
    Language English
    Publishing date 2023-01-12
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2036775-2
    ISSN 1567-1364 ; 1567-1356
    ISSN (online) 1567-1364
    ISSN 1567-1356
    DOI 10.1093/femsyr/foad002
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  4. Article ; Online: Easy Modular Integrative fuSion-ready Expression (Easy-MISE) Toolkit for Fast Engineering of Heterologous Productions in

    Maestroni, Letizia / Butti, Pietro / Milanesi, Riccardo / Pagliari, Stefania / Campone, Luca / Serra, Immacolata / Branduardi, Paola

    ACS synthetic biology

    2023  Volume 12, Issue 5, Page(s) 1508–1519

    Abstract: Nowadays, the ... ...

    Abstract Nowadays, the yeast
    MeSH term(s) Saccharomyces cerevisiae/genetics ; Saccharomyces cerevisiae/metabolism ; Fermentation ; Metabolic Engineering ; CRISPR-Cas Systems
    Language English
    Publishing date 2023-04-14
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 2161-5063
    ISSN (online) 2161-5063
    DOI 10.1021/acssynbio.3c00015
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  5. Book ; Online: Catalytic, enantioselective carbon-phosphorus bond forming reactions

    Butti, Pietro

    2009  

    Abstract: Diss., Eidgenössische Technische Hochschule ETH Zürich, Nr. 18839, ... ...

    Abstract Diss., Eidgenössische Technische Hochschule ETH Zürich, Nr. 18839, 2009
    Keywords PHOSPHINE (ORGANISCHE CHEMIE) ; ASYMMETRISCHE CHEMISCHE SYNTHESE + ENANTIOSELEKTIVE SYNTHESE (ORGANISCHE SYNTHESE) ; ASYMMETRISCHE KATALYSE (CHEMISCHE REAKTIONEN) ; CYCLOPENTADIENYLKOMPLEXE (KOMPLEXCHEMIE) ; NICKELKOMPLEXE (KOMPLEXCHEMIE) ; PALLADIUMKOMPLEXE (KOMPLEXCHEMIE)
    Language English
    Publisher Zürich, ETH
    Publishing country ch
    Document type Book ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Thesis ; Online: Catalytic, enantioselective carbon-phosphorus bond forming reactions

    Butti, Pietro

    2009  

    Keywords PHOSPHINE (ORGANISCHE CHEMIE) ; ASYMMETRISCHE CHEMISCHE SYNTHESE + ENANTIOSELEKTIVE SYNTHESE (ORGANISCHE SYNTHESE) ; ASYMMETRISCHE KATALYSE (CHEMISCHE REAKTIONEN) ; CYCLOPENTADIENYLKOMPLEXE (KOMPLEXCHEMIE) ; NICKELKOMPLEXE (KOMPLEXCHEMIE) ; PALLADIUMKOMPLEXE (KOMPLEXCHEMIE) ; PHOSPHINES (ORGANIC CHEMISTRY) ; ASYMMETRIC CHEMICAL SYNTHESIS + ENANTIOSELECTIVE SYNTHESIS (ORGANIC SYNTHESIS) ; ASYMMETRIC CATALYSIS (CHEMICAL REACTIONS) ; CYCLOPENTADIENYL COMPLEXES (COMPLEX CHEMISTRY) ; NICKEL COMPLEXES (COMPLEX CHEMISTRY) ; PALLADIUM COMPLEXES (COMPLEX CHEMISTRY) ; info:eu-repo/classification/ddc/540 ; Chemistry
    Language English
    Publisher ETH
    Publishing country ch
    Document type Thesis ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  7. Book ; Online: Spatially mapping the thermal conductivity of graphene by an opto-thermal method

    Braun, Oliver / Furrer, Roman / Butti, Pascal / Thodkar, Kishan R. / Shorubalko, Ivan / Zardo, Ilaria / Calame, Michel / Perrin, Mickael L.

    2021  

    Abstract: Mapping the thermal transport properties of materials at the nanoscale is of critical importance for optimizing heat conduction in nanoscale devices. Several methods to determine the thermal conductivity of materials have been developed, most of them ... ...

    Abstract Mapping the thermal transport properties of materials at the nanoscale is of critical importance for optimizing heat conduction in nanoscale devices. Several methods to determine the thermal conductivity of materials have been developed, most of them yielding an average value across the sample, thereby disregarding the role of local variations. Here, we present a method for the spatially-resolved assessment of the thermal conductivity of suspended graphene by using a combination of confocal Raman thermometry and a finite-element calculations-based fitting procedure. We demonstrate the working principle of our method by extracting the two-dimensional thermal conductivity map of one pristine suspended single-layer graphene sheet and one irradiated using helium ions. Our method paves the way for spatially resolving the thermal conductivity of other types of layered materials. This is particularly relevant for the design and engineering of nanoscale thermal circuits (e.g. thermal diodes).
    Keywords Condensed Matter - Mesoscale and Nanoscale Physics
    Subject code 621
    Publishing date 2021-01-28
    Publishing country us
    Document type Book ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  8. Article ; Online: Palladium-catalyzed enantioselective allylic phosphination.

    Butti, Pietro / Rochat, Raphaël / Sadow, Aaron D / Togni, Antonio

    Angewandte Chemie (International ed. in English)

    2008  Volume 47, Issue 26, Page(s) 4878–4881

    Language English
    Publishing date 2008
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2011836-3
    ISSN 1521-3773 ; 1433-7851
    ISSN (online) 1521-3773
    ISSN 1433-7851
    DOI 10.1002/anie.200801287
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  9. Article: Palladium-Catalyzed Enantioselective Allylic Phosphination

    Butti, Pietro / Rochat, Raphaël / Sadow, Aaron D / Togni, Antonio

    Angewandte Chemie. 2008 June 16, v. 47, no. 26

    2008  

    Language English
    Dates of publication 2008-0616
    Size p. 4878-4881.
    Publishing place Wiley-VCH Verlag
    Document type Article
    ZDB-ID 2011836-3
    ISSN 1521-3773 ; 1433-7851
    ISSN (online) 1521-3773
    ISSN 1433-7851
    DOI 10.1002/anie.200801287
    Database NAL-Catalogue (AGRICOLA)

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  10. Article: Primäres und sekundäres Raynaud-Syndrom

    Bollinger, A / Butti, P

    Schweizerische medizinische Wochenschrift

    1976  Volume 106, Issue 12, Page(s) 415–421

    Abstract: In the light of practical considerations a distinction is necessary between vasospastic and occlusive disease of hand and finger arteries. The vasospastic condition is usually termed Raynaud's disease and leads to recurrent ischemic attacks affecting the ...

    Title translation Primary and secondary Raynaud's syndrome.
    Abstract In the light of practical considerations a distinction is necessary between vasospastic and occlusive disease of hand and finger arteries. The vasospastic condition is usually termed Raynaud's disease and leads to recurrent ischemic attacks affecting the fingers, with early onset in life. In most instances, organic occlusions of peripheral arteries develop without initial vasospastic symptoms (exception: patients with collagen vascular disorders). Measurements of finger blood flow reported in this review show that vasospastic Raynaud's disease may be considered a variant of physiologic flow behavior in young women. Only in severe cases do cold stimuli precipitate long-lasting periods of non-measurable finger flow (venous occlusion plethysmography with finger strain gauges). In 109 personal observations the possible etiologic factors involved in organic finger artery occlusions are analyzed. They include a broad spectrum (arteriosclerosis obliterans, endangitis obliterans, collagen vascular disease, thrombocytosis, chronic occupational trauma, cryoglobulinemia). Therapy is directed at the underlying disease and the specific situation. In cases with severe ischemic symptoms, sympathectomy, intraarterial injections of reserpine, and treatment with Ancrod or fibrinolytic agents should be considered.
    MeSH term(s) Adult ; Arterial Occlusive Diseases/complications ; Arterial Occlusive Diseases/etiology ; Cold Temperature ; Collagen Diseases/complications ; Cryoglobulins ; Female ; Fibrinolytic Agents/therapeutic use ; Fingers/blood supply ; Humans ; Male ; Middle Aged ; Raynaud Disease/etiology ; Raynaud Disease/therapy ; Reserpine/therapeutic use ; Spasm/complications ; Sympathectomy ; Thromboangiitis Obliterans/complications ; Thrombocytosis/complications
    Chemical Substances Cryoglobulins ; Fibrinolytic Agents ; Reserpine (8B1QWR724A)
    Language German
    Publishing date 1976-03-20
    Publishing country Switzerland
    Document type English Abstract ; Journal Article
    ZDB-ID 200461-6
    ISSN 0036-7672
    ISSN 0036-7672
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Ua VI Zs.152: Show issues Location:
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