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  1. Article: Identification of vaccine candidates against

    Liu, Lu / Yu, Wanli / Cai, Kuojun / Ma, Siyuan / Wang, Yanfeng / Ma, Yuhui / Zhao, Hongqiong

    Heliyon

    2023  Volume 9, Issue 8, Page(s) e18623

    Abstract: Rhodococcus ... ...

    Abstract Rhodococcus equi
    Language English
    Publishing date 2023-07-25
    Publishing country England
    Document type Journal Article
    ZDB-ID 2835763-2
    ISSN 2405-8440
    ISSN 2405-8440
    DOI 10.1016/j.heliyon.2023.e18623
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Molecular detection and genetic variability of Ehrlichia canis in pet dogs in Xinjiang, China

    Qiao Mengfan / Wang Lixia / Lei Ying / Ren Yan / Cai Kuojun / Zhang Jinsheng / Zhang Zaichao / Yu Weiwei / Peng Yelong / Cai Xuepeng / Li Chongyang / Qiao Jun / Meng Qingling

    Veterinary World, Vol 13, Iss 5, Pp 916-

    2020  Volume 922

    Abstract: Background and Aim: As a tick-borne zoonotic pathogen, Ehrlichia canis has already posed a threat to public health and safety. This study aimed to clarify the prevalence and molecular characteristics of E. canis in pet dogs in Xinjiang, China. Materials ... ...

    Abstract Background and Aim: As a tick-borne zoonotic pathogen, Ehrlichia canis has already posed a threat to public health and safety. This study aimed to clarify the prevalence and molecular characteristics of E. canis in pet dogs in Xinjiang, China. Materials and Methods: A total of 297 blood samples of pet dogs and 709 skin ticks (Rhipicephalus sanguineus sensu lato) were subjected to molecular detection using PCR for E. canis 16S rRNA gene, and then, positive samples were amplified, sequenced, and phylogenetically analyzed for E. canis gp36 gene. Results: The PCR detection showed that the positive rate of PCR was 12.12% (36/297) in blood samples and 15.23% (108/709) in tick samples, respectively. Based on the phylogenetic analysis of E. canis gp36 protein, these E. canis strains in different geographical regions of the world can be divided into Genogroup I and Genogroup II. Among them, the Xinjiang epidemic strain XJ-6 and 533, 36, 1055, Kasur1, and Jake strains were clustered into subgroup 1.1 of Genogroup I, while the XJ-2, XJ-21, and XJ-35 strains and the TWN1, TWN4, CM180, and CM196 strains were closely related and belonged to subgroup 2.2 of Genogroup II, displaying high genetic diversity. Conclusion: This is the first study focusing on the molecular epidemiology of E. canis infection in pet dogs, which revealed that E. canis infection had been occurred in Xinjiang, China. More importantly, this study confirmed that the substantial variability in immunoreactive protein gp36 from E. canis strains circulating in pet dogs.
    Keywords ehrlichia canis ; genetic characteristics ; gp36 ; pet dog ; rhipicephalus sanguineus sensu lato ; Animal culture ; SF1-1100 ; Veterinary medicine ; SF600-1100
    Subject code 630
    Language English
    Publishing date 2020-05-01T00:00:00Z
    Publisher Veterinary World
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

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  3. Article ; Online: A Novel LysR Family Factor STM0859 is Associated with The Responses of

    Ma, Zhongmei / Li, N A / Ning, Chengcheng / Liu, Yucheng / Guo, Yun / Ji, Chunhui / Zhu, Xiaozhen / Meng, Qingling / Xia, Xianzhu / Zhang, Xingxing / Cai, Xuepeng / Cai, Kuojun / Jun, Qiao

    Polish journal of microbiology

    2021  Volume 70, Issue 4, Page(s) 479–487

    Abstract: Salmonella ... ...

    Abstract Salmonella enterica
    MeSH term(s) Bacterial Proteins/metabolism ; Biofilms ; Gene Expression Regulation, Bacterial ; Salmonella typhimurium/genetics ; Stress, Physiological ; Transcription Factors/genetics
    Chemical Substances Bacterial Proteins ; Transcription Factors
    Language English
    Publishing date 2021-12-23
    Publishing country Poland
    Document type Journal Article
    ZDB-ID 2234080-4
    ISSN 2544-4646 ; 1733-1331
    ISSN (online) 2544-4646
    ISSN 1733-1331
    DOI 10.33073/pjm-2021-045
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Characteristic profiles of biofilm, enterotoxins and virulence of

    Wu, Yehui / Li, Jing / Qiao, Mengfan / Meng, Dan / Meng, Qingling / Qiao, Jun / Zhang, Xingxing / Wang, Lixia / Cai, Kuojun / Zhang, Jinsheng / Zhang, Zaichao / Yu, Weiwei / Cai, Xuepeng

    Journal of veterinary science

    2019  Volume 20, Issue 6, Page(s) e74

    Abstract: As an important zoonotic pathogen, ...

    Abstract As an important zoonotic pathogen,
    MeSH term(s) Animals ; Anti-Bacterial Agents/pharmacology ; Biofilms ; Cattle ; Cattle Diseases/microbiology ; China ; Dairying ; Drug Resistance, Bacterial/genetics ; Enterotoxins/chemistry ; Female ; Staphylococcal Infections/microbiology ; Staphylococcal Infections/veterinary ; Staphylococcus aureus/drug effects ; Staphylococcus aureus/genetics ; Staphylococcus aureus/pathogenicity ; Staphylococcus aureus/physiology ; Virulence
    Chemical Substances Anti-Bacterial Agents ; Enterotoxins
    Language English
    Publishing date 2019-11-27
    Publishing country Korea (South)
    Document type Journal Article
    ZDB-ID 2125308-0
    ISSN 1976-555X ; 1229-845X
    ISSN (online) 1976-555X
    ISSN 1229-845X
    DOI 10.4142/jvs.2019.20.e74
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  5. Article: [Effects of the deletion of RsbV gene on virulence of Listeria monocytogenes].

    Zhang, Zaichao / Meng, Qingling / Qiao, Jun / Chen, Chuangfu / Cai, Xuepeng / Yang, Lihong / Wang, Weisheng / Cai, Kuojun

    Wei sheng wu xue bao = Acta microbiologica Sinica

    2013  Volume 53, Issue 1, Page(s) 59–65

    Abstract: Objective: To study the effect of RsbV gene deletion on the virulence of Listeria monocytogenes.: Methods: The fragment with RsbV deletion was generated by gene overlap extension PCR (SOE-PCR) and the mutant with RsbV deletion was obtained by ... ...

    Abstract Objective: To study the effect of RsbV gene deletion on the virulence of Listeria monocytogenes.
    Methods: The fragment with RsbV deletion was generated by gene overlap extension PCR (SOE-PCR) and the mutant with RsbV deletion was obtained by homologous recombination based on the wild strain LM-XS5. The differences in virulence between the two strains were determined by LD50, bacterial counts in liver and spleen, and qRT-PCR experiments.
    Results: LD50 of the RsbV gene-deleted strain was 10(4) higher than that of the wild strain. The numbers of gene-deleted strain in the mouse's liver and spleen were significantly fewer than that of the wild strain (P < 0.05). Results of qRT-PCR show that four virulence factors' expression levels of the RsbV gene-deleted strain were significantly lower than that of the wild strain (P < 0.05). The RsbV gene-deleted strain induced a strong immune response in mice against the wild strain.
    Conclusion: RsbV regulates the expression of four virulence gene (inlA, LLO, PlcA and PrfA) of Listeria monocytogenes; The virulence of the RsbV gene-deleted strain is significantly reduced, but it still has good immunogenicity.
    MeSH term(s) Animals ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Base Sequence ; Gene Deletion ; Gene Expression Regulation, Bacterial ; Humans ; Listeria monocytogenes/genetics ; Listeria monocytogenes/metabolism ; Listeria monocytogenes/pathogenicity ; Listeriosis/microbiology ; Mice ; Molecular Sequence Data ; Virulence ; Virulence Factors/genetics ; Virulence Factors/metabolism
    Chemical Substances Bacterial Proteins ; Virulence Factors
    Language Chinese
    Publishing date 2013-01-04
    Publishing country China
    Document type English Abstract ; Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 412683-x
    ISSN 0001-6209 ; 0098-9150
    ISSN 0001-6209 ; 0098-9150
    Database MEDical Literature Analysis and Retrieval System OnLINE

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