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  1. AU="Caleiro, Giovana Santos"
  2. AU="Sadia, Khulah"
  3. AU="Hong, Ka Young"
  4. AU="Mauricio Duque-Ramírez"
  5. AU="Ajjur, Salah B"
  6. AU="Maiti, Kaushik"
  7. AU="Sun, Haoqi"
  8. AU="Jie Lin"
  9. AU="Jiang Huang" AU="Jiang Huang"
  10. AU="Yongliang Zhang"
  11. AU="Ernest, C Steven"
  12. AU="Axel Haferkamp"
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  1. Artikel: Phenotypic and Genotypic Characterization of

    Kurihara, Mariana Neri Lucas / Santos, Ingrid Nayara Marcelino / Eisen, Ana Karolina Antunes / Caleiro, Giovana Santos / Araújo, Jansen de / Sales, Romário Oliveira de / Pignatari, Antônio Carlos / Salles, Mauro José

    Microorganisms

    2023  Band 11, Heft 10

    Abstract: Specific virulence factors that likely ... ...

    Abstract Specific virulence factors that likely influence
    Sprache Englisch
    Erscheinungsdatum 2023-10-20
    Erscheinungsland Switzerland
    Dokumenttyp Journal Article
    ZDB-ID 2720891-6
    ISSN 2076-2607
    ISSN 2076-2607
    DOI 10.3390/microorganisms11102594
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  2. Artikel ; Online: Phylogenetics, Epidemiology and Temporal Patterns of Dengue Virus in Araraquara, São Paulo State.

    de Souza, Caio Santos / Caleiro, Giovana Santos / Claro, Ingra Morales / de Jesus, Jaqueline Goes / Coletti, Thaís Moura / da Silva, Camila Alves Maia / Costa, Ângela Aparecida / Inenami, Marta / Ribeiro, Andreia C / Felix, Alvina Clara / de Paula, Anderson Vicente / Figueiredo, Walter M / de Albuquerque Luna, Expedito José / Sabino, Ester C / Romano, Camila M

    Viruses

    2024  Band 16, Heft 2

    Abstract: Dengue virus (DENV) is a prominent arbovirus with global spread, causing approximately 390 million infections each year. In Brazil, yearly epidemics follow a well-documented pattern of serotype replacement every three to four years on average. Araraquara, ...

    Abstract Dengue virus (DENV) is a prominent arbovirus with global spread, causing approximately 390 million infections each year. In Brazil, yearly epidemics follow a well-documented pattern of serotype replacement every three to four years on average. Araraquara, located in the state of São Paulo, has faced significant impacts from DENV epidemics since the emergence of DENV-1 in 2010. The municipality then transitioned from low to moderate endemicity in less than 10 years. Yet, there remains an insufficient understanding of virus circulation dynamics, particularly concerning DENV-1, in the region, as well as the genetic characteristics of the virus. To address this, we sequenced 37 complete or partial DENV-1 genomes sampled from 2015 to 2022 in Araraquara. Then, using also Brazilian and worldwide DENV-1 sequences we reconstructed the evolutionary history of DENV-1 in Araraquara and estimated the time to the most recent common ancestor (tMRCA) for serotype 1, for genotype V and its main lineages. Within the last ten years, there have been at least three introductions of genotype V in Araraquara, distributed in two main lineages (L Ia and L Ib, and L II). The tMRCA for the first sampled lineage (2015/2016 epidemics) was approximately 15 years ago (in 2008). Crucially, our analysis challenges existing assumptions regarding the emergence time of the DENV-1 genotypes, suggesting that genotype V might have diverged more recently than previously described. The presence of the two lineages of genotype V in the municipality might have contributed to the extended persistence of DENV-1 in the region.
    Mesh-Begriff(e) Humans ; Phylogeny ; Dengue Virus/genetics ; Dengue/epidemiology ; Brazil/epidemiology ; Genotype
    Sprache Englisch
    Erscheinungsdatum 2024-02-09
    Erscheinungsland Switzerland
    Dokumenttyp Journal Article
    ZDB-ID 2516098-9
    ISSN 1999-4915 ; 1999-4915
    ISSN (online) 1999-4915
    ISSN 1999-4915
    DOI 10.3390/v16020274
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  3. Artikel ; Online: Applying a pan-flavivirus RT-qPCR assay in Brazilian public health surveillance.

    Cunha, Mariana Sequetin / Luchs, Adriana / Dos Santos, Fabiana Cristina Pereira / Caleiro, Giovana Santos / Nogueira, Maurício Lacerda / Maiorka, Paulo César

    Archives of virology

    2020  Band 165, Heft 8, Seite(n) 1863–1868

    Abstract: The aim of this study was to improve flavivirus field monitoring in Brazil using a reliable probe-based RT-qPCR assay. Standard flavivirus strains were employed to evaluate the performance of the assay, and its applicability was evaluated using 235 ... ...

    Abstract The aim of this study was to improve flavivirus field monitoring in Brazil using a reliable probe-based RT-qPCR assay. Standard flavivirus strains were employed to evaluate the performance of the assay, and its applicability was evaluated using 235 stored pools of Culicidae samples collected between 1993 and 1997 and in 2016. Flavivirus species were identified by sequencing. Sixteen (6.8%) samples tested positive: Ilheus virus, Iguape virus, and Saint Louis encephalitis virus were identified in historical specimens from 1993-1994, while insect-specific flaviviruses were detected in the samples from 2016. This approach was demonstrated to be accurate for flavivirus detection and characterization, and it can be successfully applied for vector surveillance and for monitoring and discovery of insect specific flaviviruses.
    Mesh-Begriff(e) Animals ; Brazil ; Flavivirus/genetics ; Phylogeny ; Public Health Surveillance/methods ; Real-Time Polymerase Chain Reaction/methods
    Sprache Englisch
    Erscheinungsdatum 2020-05-30
    Erscheinungsland Austria
    Dokumenttyp Journal Article
    ZDB-ID 7491-3
    ISSN 1432-8798 ; 0304-8608
    ISSN (online) 1432-8798
    ISSN 0304-8608
    DOI 10.1007/s00705-020-04680-w
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  4. Artikel: Applying a pan-flavivirus RT-qPCR assay in Brazilian public health surveillance

    Cunha, Mariana Sequetin / Luchs, Adriana / dos Santos, Fabiana Cristina Pereira / Caleiro, Giovana Santos / Nogueira, Maurício Lacerda / Maiorka, Paulo César

    Archives of virology. 2020 Aug., v. 165, no. 8

    2020  

    Abstract: The aim of this study was to improve flavivirus field monitoring in Brazil using a reliable probe-based RT-qPCR assay. Standard flavivirus strains were employed to evaluate the performance of the assay, and its applicability was evaluated using 235 ... ...

    Abstract The aim of this study was to improve flavivirus field monitoring in Brazil using a reliable probe-based RT-qPCR assay. Standard flavivirus strains were employed to evaluate the performance of the assay, and its applicability was evaluated using 235 stored pools of Culicidae samples collected between 1993 and 1997 and in 2016. Flavivirus species were identified by sequencing. Sixteen (6.8%) samples tested positive: Ilheus virus, Iguape virus, and Saint Louis encephalitis virus were identified in historical specimens from 1993-1994, while insect-specific flaviviruses were detected in the samples from 2016. This approach was demonstrated to be accurate for flavivirus detection and characterization, and it can be successfully applied for vector surveillance and for monitoring and discovery of insect specific flaviviruses.
    Schlagwörter Aroa virus ; Culicidae ; Ilheus virus ; Saint Louis encephalitis virus ; insects ; monitoring ; public health ; virology ; Brazil
    Sprache Englisch
    Erscheinungsverlauf 2020-08
    Umfang p. 1863-1868.
    Erscheinungsort Springer Vienna
    Dokumenttyp Artikel
    Anmerkung NAL-AP-2-clean
    ZDB-ID 7491-3
    ISSN 1432-8798 ; 0304-8608
    ISSN (online) 1432-8798
    ISSN 0304-8608
    DOI 10.1007/s00705-020-04680-w
    Datenquelle NAL Katalog (AGRICOLA)

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  5. Artikel: Genomic evidence of yellow fever virus in Aedes scapularis, southeastern Brazil, 2016

    Cunha, Mariana Sequetin / Faria, Nuno Rodrigues / Caleiro, Giovana Santos / Candido, Darlan Silva / Hill, Sarah C / Claro, Ingra Morales / da Costa, Antônio Charlys / Nogueira, Juliana Silva / Maeda, Adriana Yurika / da Silva, Fernanda Gisele / de Souza, Renato Pereira / Spinola, Roberta / Tubaki, Rosa Maria / de Menezes, Regiane Maria Tironi / Abade, Leandro / Mucci, Luís Filipe / Timenetsky, Maria do Carmo Sampaio Tavares / Sabino, Esther

    Acta tropica. 2020 May, v. 205

    2020  

    Abstract: The southeastern region of Brazil has recently experienced the largest yellow fever disease outbreak in decades. Since July 2016 epizootic events were reported in São Paulo state's north region, where 787 Culicidae were captured as part of public health ... ...

    Abstract The southeastern region of Brazil has recently experienced the largest yellow fever disease outbreak in decades. Since July 2016 epizootic events were reported in São Paulo state's north region, where 787 Culicidae were captured as part of public health surveillance efforts and tested using real-time quantitative PCR. One Aedes scapularis pool collected in November 2016 in an agriculture area in Urupês city tested positive for YFV-RNA. Using a validated multiplex PCR approach we were able to recover a complete virus genome sequence from this pool. Phylogenetic analysis of the novel strain and publicly available data indicates that the belongs to the South American genotype 1 clade circulating in Sao Paulo state and is basal to the recent outbreak clade in southeast Brazil. Our findings highlight the need of additional studies, including vector competence studies, to disentangle the role of Aedes scapularis in yellow fever transmission in the Americas.
    Schlagwörter Aedes ; Yellow fever virus ; agricultural land ; disease outbreaks ; epizootic diseases ; genomics ; genotype ; monitoring ; nucleotide sequences ; phylogeny ; public health ; quantitative polymerase chain reaction ; vector competence ; viruses ; yellow fever ; Brazil ; North America
    Sprache Englisch
    Erscheinungsverlauf 2020-05
    Erscheinungsort Elsevier B.V.
    Dokumenttyp Artikel
    ZDB-ID 210415-5
    ISSN 1873-6254 ; 0001-706X
    ISSN (online) 1873-6254
    ISSN 0001-706X
    DOI 10.1016/j.actatropica.2020.105390
    Datenquelle NAL Katalog (AGRICOLA)

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  6. Artikel ; Online: Possible non-sylvatic transmission of yellow fever between non-human primates in São Paulo city, Brazil, 2017-2018.

    Cunha, Mariana Sequetin / Tubaki, Rosa Maria / de Menezes, Regiane Maria Tironi / Pereira, Mariza / Caleiro, Giovana Santos / Coelho, Esmenia / Saad, Leila Del Castillo / Fernandes, Natalia Coelho Couto de Azevedo / Guerra, Juliana Mariotti / Nogueira, Juliana Silva / Summa, Juliana Laurito / Coimbra, Amanda Aparecida Cardoso / Zwarg, Ticiana / Witkin, Steven S / Mucci, Luís Filipe / Timenetsky, Maria do Carmo Sampaio Tavares / Sabino, Ester Cerdeira / de Deus, Juliana Telles

    Scientific reports

    2020  Band 10, Heft 1, Seite(n) 15751

    Abstract: Yellow Fever (YF) is a severe disease caused by Yellow Fever Virus (YFV), endemic in some parts of Africa and America. In Brazil, YFV is maintained by a sylvatic transmission cycle involving non-human primates (NHP) and forest canopy-dwelling mosquitoes, ...

    Abstract Yellow Fever (YF) is a severe disease caused by Yellow Fever Virus (YFV), endemic in some parts of Africa and America. In Brazil, YFV is maintained by a sylvatic transmission cycle involving non-human primates (NHP) and forest canopy-dwelling mosquitoes, mainly Haemagogus-spp and Sabethes-spp. Beginning in 2016, Brazil faced one of the largest Yellow Fever (YF) outbreaks in recent decades, mainly in the southeastern region. In São Paulo city, YFV was detected in October 2017 in Aloutta monkeys in an Atlantic Forest area. From 542 NHP, a total of 162 NHP were YFV positive by RT-qPCR and/or immunohistochemistry, being 22 Callithrix-spp. most from urban areas. Entomological collections executed did not detect the presence of strictly sylvatic mosquitoes. Three mosquito pools were positive for YFV, 2 Haemagogus leucocelaenus, and 1 Aedes scapularis. In summary, YFV in the São Paulo urban area was detected mainly in resident marmosets, and synanthropic mosquitoes were likely involved in viral transmission.
    Mesh-Begriff(e) Animals ; Brazil/epidemiology ; Cities/epidemiology ; Disease Outbreaks ; Mosquito Vectors/physiology ; Phylogeny ; Primates/virology ; Yellow Fever/epidemiology ; Yellow Fever/transmission
    Sprache Englisch
    Erscheinungsdatum 2020-09-25
    Erscheinungsland England
    Dokumenttyp Journal Article
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-020-72794-x
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  7. Artikel ; Online: Genomic evidence of yellow fever virus in Aedes scapularis, southeastern Brazil, 2016.

    Cunha, Mariana Sequetin / Faria, Nuno Rodrigues / Caleiro, Giovana Santos / Candido, Darlan Silva / Hill, Sarah C / Claro, Ingra Morales / da Costa, Antônio Charlys / Nogueira, Juliana Silva / Maeda, Adriana Yurika / da Silva, Fernanda Gisele / de Souza, Renato Pereira / Spinola, Roberta / Tubaki, Rosa Maria / de Menezes, Regiane Maria Tironi / Abade, Leandro / Mucci, Luís Filipe / Timenetsky, Maria do Carmo Sampaio Tavares / Sabino, Esther

    Acta tropica

    2020  Band 205, Seite(n) 105390

    Abstract: The southeastern region of Brazil has recently experienced the largest yellow fever disease outbreak in decades. Since July 2016 epizootic events were reported in São Paulo state's north region, where 787 Culicidae were captured as part of public health ... ...

    Abstract The southeastern region of Brazil has recently experienced the largest yellow fever disease outbreak in decades. Since July 2016 epizootic events were reported in São Paulo state's north region, where 787 Culicidae were captured as part of public health surveillance efforts and tested using real-time quantitative PCR. One Aedes scapularis pool collected in November 2016 in an agriculture area in Urupês city tested positive for YFV-RNA. Using a validated multiplex PCR approach we were able to recover a complete virus genome sequence from this pool. Phylogenetic analysis of the novel strain and publicly available data indicates that the belongs to the South American genotype 1 clade circulating in Sao Paulo state and is basal to the recent outbreak clade in southeast Brazil. Our findings highlight the need of additional studies, including vector competence studies, to disentangle the role of Aedes scapularis in yellow fever transmission in the Americas.
    Mesh-Begriff(e) Aedes/classification ; Aedes/virology ; Animals ; Brazil/epidemiology ; Genome, Viral ; Humans ; Mosquito Vectors/virology ; Phylogeny ; Real-Time Polymerase Chain Reaction ; Yellow Fever/epidemiology ; Yellow Fever/transmission ; Yellow fever virus/genetics
    Sprache Englisch
    Erscheinungsdatum 2020-02-07
    Erscheinungsland Netherlands
    Dokumenttyp Journal Article
    ZDB-ID 210415-5
    ISSN 1873-6254 ; 0001-706X
    ISSN (online) 1873-6254
    ISSN 0001-706X
    DOI 10.1016/j.actatropica.2020.105390
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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