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  1. Book ; Online: Parasites and Wildlife

    Calero-Bernal, Rafael / García-Bocanegra, Ignacio

    2023  

    Keywords Medicine ; Epidemiology & medical statistics ; arctic fox ; Iceland ; vector-borne bacteria ; vector-borne protozoan parasites ; climate change ; Encephalitozoon intestinalis ; E. hellem ; E. cuniculi ; Enterocytozoon bieneusi ; European wild rabbit ; Iberian hare ; zoonotic ; foodborne ; parasite ; long-term study ; protozoan ; shared infections ; zoonoses ; wildlife-livestock interface ; Echinococcus multilocularis ; PCR ; DNA extraction ; feces ; Acari ; gamasid mite ; Ornithonyssus bacoti ; distribution ; host selection ; Yunnan ; China ; game meat ; Trichinella ; wildlife ; zoonosis ; wild birds ; coccidian ; molecular ; apicomplexa ; marine ; Chile ; helminths ; land conversion ; Rodentia ; small mammals ; Sarcocystis ; cattle ; mustelidae ; life cycle ; cox1 ; molecular identification ; striped dolphin ; tissue cysts ; neuropathology ; Toxoplasma gondii ; Sarcocystis-like ; genotype ; 18S rRNA ; Babesia ; rodents ; voles ; mice ; Lithuania ; protists ; non-human primates ; endangered ; conservation ; genotyping ; epidemiology ; seasonality ; n/a
    Language English
    Size 1 electronic resource (174 pages)
    Publisher MDPI - Multidisciplinary Digital Publishing Institute
    Publishing place Basel
    Document type Book ; Online
    Note English
    HBZ-ID HT030375159
    ISBN 9783036568539 ; 3036568530
    Database ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

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  2. Article: Parasites and Wildlife.

    Calero-Bernal, Rafael / García-Bocanegra, Ignacio

    Animals : an open access journal from MDPI

    2023  Volume 13, Issue 4

    Abstract: Macro and micro-parasites are integrated into ecosystems worldwide and are considered important elements of biodiversity [ ... ]. ...

    Abstract Macro and micro-parasites are integrated into ecosystems worldwide and are considered important elements of biodiversity [...].
    Language English
    Publishing date 2023-02-10
    Publishing country Switzerland
    Document type Editorial
    ZDB-ID 2606558-7
    ISSN 2076-2615
    ISSN 2076-2615
    DOI 10.3390/ani13040628
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Parasites and Wildlife

    Calero-Bernal, Rafael / García-Bocanegra, Ignacio

    Animals. 2023 Feb. 10, v. 13, no. 4

    2023  

    Abstract: Macro and micro-parasites are integrated into ecosystems worldwide and are considered important elements of biodiversity [ ... ] ...

    Abstract Macro and micro-parasites are integrated into ecosystems worldwide and are considered important elements of biodiversity [...]
    Keywords animals ; biodiversity ; ecosystems ; parasites ; wildlife
    Language English
    Dates of publication 2023-0210
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article ; Online
    ZDB-ID 2606558-7
    ISSN 2076-2615
    ISSN 2076-2615
    DOI 10.3390/ani13040628
    Database NAL-Catalogue (AGRICOLA)

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  4. Article ; Online: Unifying Virulence Evaluation in

    Calero-Bernal, Rafael / Fernández-Escobar, Mercedes / Katzer, Frank / Su, Chunlei / Ortega-Mora, Luis Miguel

    Frontiers in cellular and infection microbiology

    2022  Volume 12, Page(s) 868727

    Abstract: Toxoplasma ... ...

    Abstract Toxoplasma gondii
    MeSH term(s) Animals ; Genotype ; Mice ; Phenotype ; Toxoplasma ; Toxoplasmosis, Animal ; Virulence/genetics
    Language English
    Publishing date 2022-04-28
    Publishing country Switzerland
    Document type Journal Article ; Review ; Research Support, Non-U.S. Gov't
    ZDB-ID 2619676-1
    ISSN 2235-2988 ; 2235-2988
    ISSN (online) 2235-2988
    ISSN 2235-2988
    DOI 10.3389/fcimb.2022.868727
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Sarcocystis

    Marandykina-Prakienė, Alina / Butkauskas, Dalius / Gudiškis, Naglis / Juozaitytė-Ngugu, Evelina / Bagdonaitė, Dovilė Laisvūnė / Kirjušina, Muza / Calero-Bernal, Rafael / Prakas, Petras

    Veterinary sciences

    2023  Volume 10, Issue 8

    Abstract: Contradictory data is available on the intermediate host specificity ... ...

    Abstract Contradictory data is available on the intermediate host specificity of
    Language English
    Publishing date 2023-08-11
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2768971-2
    ISSN 2306-7381 ; 2306-7381
    ISSN (online) 2306-7381
    ISSN 2306-7381
    DOI 10.3390/vetsci10080520
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: Anti-

    Calero-Bernal, Rafael / Gennari, Solange María / Cano, Santiago / Salas-Fajardo, Martha Ynés / Ríos, Arantxa / Álvarez-García, Gema / Ortega-Mora, Luis Miguel

    Pathogens (Basel, Switzerland)

    2023  Volume 12, Issue 12

    Abstract: Toxoplasmosis has a major impact on animal and public health. Information regarding the seroprevalence of ... ...

    Abstract Toxoplasmosis has a major impact on animal and public health. Information regarding the seroprevalence of human
    Language English
    Publishing date 2023-12-08
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2695572-6
    ISSN 2076-0817
    ISSN 2076-0817
    DOI 10.3390/pathogens12121430
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Short-term culture adaptation of Toxoplasma gondii archetypal II and III field isolates affects cystogenic capabilities and modifies virulence in mice.

    Colos-Arango, Alicia / Largo-de la Torre, Andrea / Calero-Bernal, Rafael / Ortega-Mora, Luis-Miguel / Regidor-Cerrillo, Javier

    International journal for parasitology

    2023  Volume 53, Issue 9, Page(s) 491–504

    Abstract: Most Toxoplasma gondii research has been carried out using strains maintained in the laboratory for long periods of time. Long-term passage in mice or cell culture influences T. gondii phenotypic traits such as the capability to produce oocysts in cats ... ...

    Abstract Most Toxoplasma gondii research has been carried out using strains maintained in the laboratory for long periods of time. Long-term passage in mice or cell culture influences T. gondii phenotypic traits such as the capability to produce oocysts in cats and virulence in mice. In this work, we investigated the effect of cell culture adaptation in the short term for recently obtained type II (TgShSp1 (Genotype ToxoDB#3), TgShSp2 (#1), TgShSp3 (#3) and TgShSp16 (#3)) and type III (#2) isolates (TgShSp24 and TgPigSp1). With this purpose, spontaneous and alkaline stress-induced cyst formation in Vero cells during 40 passages, from passage 10 (p10) to 50 (p50), and isolate virulence at p10 versus p50 were studied using a harmonized bioassay method in Swiss/CD1 mice. T. gondii cell culture maintenance showed a drastic loss of spontaneous and induced production of mature cysts after ≈25-30 passages. The TgShSp1, TgShSp16 and TgShSp24 isolates failed to generate spontaneously formed mature cysts at p50. Limited cyst formation was associated with an increase in parasite growth and a shorter lytic cycle. In vitro maintenance also modified T. gondii virulence in mice at p50 with events of exacerbation, increasing cumulative morbidity for TgShSp2 and TgShSp3 isolates and mortality for TgShSp24 and TgPigSp1 isolates, or attenuation, with absence of mortality and severe clinical signs for TgShSp16, and better control of the infection with the lowest parasite and cyst burdens in lungs and brain for the TgShSp1 isolate. The present findings show deep changes in relevant phenotypic traits in laboratory-adapted T. gondii isolates and open new discussion about their use for inferring keys to parasite biology and virulence.
    MeSH term(s) Chlorocebus aethiops ; Animals ; Mice ; Cats ; Toxoplasma ; Toxoplasmosis, Animal/parasitology ; Virulence ; Vero Cells ; Genotype ; Antibodies, Protozoan
    Chemical Substances Antibodies, Protozoan
    Language English
    Publishing date 2023-04-12
    Publishing country England
    Document type Journal Article
    ZDB-ID 120518-3
    ISSN 1879-0135 ; 0020-7519
    ISSN (online) 1879-0135
    ISSN 0020-7519
    DOI 10.1016/j.ijpara.2023.03.004
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 789: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  8. Article ; Online: Optimization of the most widely used serological tests for a harmonized diagnosis of Toxoplasma gondii infection in domestic pigs.

    López-Ureña, Nadia María / Calero-Bernal, Rafael / González-Fernández, Nuria / Blaga, Radu / Koudela, Bretislav / Ortega-Mora, Luis Miguel / Álvarez-García, Gema

    Veterinary parasitology

    2023  Volume 322, Page(s) 110024

    Abstract: The intake of Toxoplasma gondii tissue cysts through raw or undercooked pork meat is one of the main infection sources for humans. Thus, surveillance is recommended to control and prevent infection in domestic pigs. However, the lack of comparative ... ...

    Abstract The intake of Toxoplasma gondii tissue cysts through raw or undercooked pork meat is one of the main infection sources for humans. Thus, surveillance is recommended to control and prevent infection in domestic pigs. However, the lack of comparative studies hampers the updating of their performance and the comparison of seroprevalence data. Therefore, the aim of this study was to develop and validate three in-house tests and accomplish a comparative analysis of the most widely used serological tests employed in pigs. A panel of sera from pigs experimentally infected with either oocysts or tissue cysts from type II and III isolates (n = 158) was used to develop and validate a tachyzoite-based Western blot assay. Then, this technique was used as a reference to develop and preliminary validate a lyophilized tachyzoite-based enzyme-linked immunosorbent assay and an immunofluorescence antibody test. Next, a comparative study of the three in-house tests and three widely used commercial ELISAs (IDScreen®, PrioCHECK™ and Pigtype®) was accomplished with the abovementioned sera together with an additional serum panel of pigs experimentally infected with oocysts from the type II isolate (n = 44) and a panel of naturally infected pigs (n = 244). The results obtained by the majority of the tests were regarded as reference, and data analyses included TG-ROC calculations and agreement tests. Finally, the kinetics of anti-T. gondii IgGs from experimentally infected pigs was analyzed. Excellent sensitivity (Se) and specificity (Sp) values (≥ 93%) and moderate to near perfect agreement (k = 0.63-0.91) were observed using sera from experimental infections without requiring further readjustment, except for PrioCHECK (100% Se, 73% Sp). However, the Se of IDScreen® (87%) and TgSALUVET WB (71%) and the Sp of PrioCHECK (72%) were slightly or notably reduced when sera from naturally infected animals were analyzed, which also influenced the kappa values (k = 0.30-0.91). Cutoff readjustments increased the Se and Sp values to equal to or above 97% for all tests, except for TgSALUVET WB, which can be used as a reference for initial validation of tests, but it is not recommended for routine diagnosis. Seroconversion was recorded from two weeks post-infection by most of the tests, with significantly higher IgG levels in sera from pigs infected with the T. gondii type III vs. type II isolate. Again, differences regarding the test employed were observed. Differences in the diagnostic performance among tests evidenced the need to harmonize serological techniques to obtain comparable and reliable results.
    Language English
    Publishing date 2023-09-09
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 196831-2
    ISSN 1873-2550 ; 0304-4017
    ISSN (online) 1873-2550
    ISSN 0304-4017
    DOI 10.1016/j.vetpar.2023.110024
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4380: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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  9. Article ; Online: A comparative study of serological tests used in the diagnosis of Toxoplasma gondii infection in small ruminants evidenced the importance of cross-reactions for harmonizing diagnostic performance.

    López-Ureña, Nadia María / Calero-Bernal, Rafael / Vázquez-Calvo, Ángela / Sánchez-Sánchez, Roberto / Ortega-Mora, Luis Miguel / Álvarez-García, Gema

    Research in veterinary science

    2023  Volume 165, Page(s) 105052

    Abstract: Toxoplasma gondii is a major foodborne zoonotic pathogen that can be transmitted through the consumption of raw or undercooked meat of small ruminants, among others. Serology has been suggested as an epidemiological indicator and several tests are ... ...

    Abstract Toxoplasma gondii is a major foodborne zoonotic pathogen that can be transmitted through the consumption of raw or undercooked meat of small ruminants, among others. Serology has been suggested as an epidemiological indicator and several tests are available nowadays. However, there is no comparative study with the most used ones. Therefore, the objective of this study was to develop and validate two in-house tests (Western blot -TgSALUVET WB- and ELISA -TgSALUVET ELISA 2.0-) and perform a comparative study including such tests and four commercial ELISA kits (IDScreen®, PrioCHECK®, Pigtype® and IDEXX). First, a specific pattern of recognition of immunodominant antigens by TgSALUVET WB was determined with serum panels of noninfected sheep and sheep infected with T. gondii or Neospora caninum. Next, TgSALUVET WB was used as a reference to preliminary validate TgSALUVET ELISA 2.0 using sera from sheep and goats naturally infected with T. gondii. Then, the abovementioned sheep serum panels were analyzed by all tests and subjected to TG-ROC analyses and agreement tests, and cross-reactivity with the anti-N. caninum IgGs was studied. All the techniques were accurate enough for the cutoff values initially suggested with all serum panels (Se and Sp ≥ 94%), except for PrioCHECK®, which showed 83% Sp. However, a cutoff readjustment improved their diagnostic performance. Additionally, cross-reactions between anti-N. caninum antibodies and T. gondii antigens were detected with all tests. Thus, a second cutoff readjustment was carried out and the use of both readjusted cutoff values is recommended to obtain comparable data and avoid false-positive results.
    MeSH term(s) Sheep ; Animals ; Toxoplasmosis, Animal/diagnosis ; Antibodies, Protozoan ; Ruminants ; Goats ; Toxoplasma ; Enzyme-Linked Immunosorbent Assay/veterinary ; Serologic Tests/veterinary ; Neospora ; Seroepidemiologic Studies ; Coccidiosis/diagnosis ; Coccidiosis/veterinary ; Goat Diseases/diagnosis
    Chemical Substances Antibodies, Protozoan
    Language English
    Publishing date 2023-10-13
    Publishing country England
    Document type Journal Article
    ZDB-ID 840961-4
    ISSN 1532-2661 ; 0034-5288
    ISSN (online) 1532-2661
    ISSN 0034-5288
    DOI 10.1016/j.rvsc.2023.105052
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.B 673: Show issues Location:
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  10. Article ; Online: First report of Sarcocystis halieti (Apicomplexa) in bearded vulture (Gypaetus barbatus).

    Prakas, Petras / Estruch, Josep / Velarde, Roser / Ilgūnas, Mikas / Šneideris, Donatas / Nicolás-Francisco, Olga / Marco, Ignasi / Calero-Bernal, Rafael

    Veterinary research communications

    2023  Volume 48, Issue 1, Page(s) 541–546

    Abstract: At least three Sarcocystis species (S. falcatula, S. halieti and S. wobeseri-like) have been detected infecting raptorial birds. By histopathology and PCR-sequencing of the ITS1 marker, S. halieti was detected in a bearded vulture (Gypaetus barbatus) and ...

    Abstract At least three Sarcocystis species (S. falcatula, S. halieti and S. wobeseri-like) have been detected infecting raptorial birds. By histopathology and PCR-sequencing of the ITS1 marker, S. halieti was detected in a bearded vulture (Gypaetus barbatus) and a black kite (Milvus migrans) from the Catalonia region in North Spain. The 241 bp-long sequences obtained from the Sarcocystis organisms detected in both raptors showed 97.5-99.6% and 97.9-100% similarity with those of previously identified S. halieti; also, the phylogenetic trees generated placed the identified sequences together with other sequences of S. halieti available in GenBank. In sum, the description of the bearded vulture as a new intermediate host for S. halieti adds new insights on the complex epidemiology of the genus involving avian hosts.
    MeSH term(s) Animals ; Sarcocystis/genetics ; Sarcocystosis/veterinary ; Sarcocystosis/epidemiology ; Phylogeny ; Birds ; Spain
    Language English
    Publishing date 2023-08-09
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 406735-6
    ISSN 1573-7446 ; 0165-7380
    ISSN (online) 1573-7446
    ISSN 0165-7380
    DOI 10.1007/s11259-023-10191-1
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 3453: Show issues Location:
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