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  1. Article ; Online: Sequencing by avidity enables high accuracy with low reagent consumption.

    Arslan, Sinan / Garcia, Francisco J / Guo, Minghao / Kellinger, Matthew W / Kruglyak, Semyon / LeVieux, Jake A / Mah, Adeline H / Wang, Haosen / Zhao, Junhua / Zhou, Chunhong / Altomare, Andrew / Bailey, John / Byrne, Matthew B / Chang, Chiting / Chen, Steve X / Cho, Byungrae / Dennler, Claudia N / Dien, Vivian T / Fuller, Derek /
    Kelley, Ryan / Khandan, Omid / Klein, Michael G / Kim, Michael / Lajoie, Bryan R / Lin, Bill / Liu, Yu / Lopez, Tyler / Mains, Peter T / Price, Andrew D / Robertson, Samantha R / Taylor-Weiner, Hermes / Tippana, Ramreddy / Tomaney, Austin B / Zhang, Su / Abtahi, Minna / Ambroso, Mark R / Bajari, Rosita / Bellizzi, Ava M / Benitez, Chris B / Berard, Daniel R / Berti, Lorenzo / Blease, Kelly N / Blum, Angela P / Boddicker, Andrew M / Bondar, Leo / Brown, Chris / Bui, Chris A / Calleja-Aguirre, Juan / Cappa, Kevin / Chan, Joshua / Chang, Victor W / Charov, Katherine / Chen, Xiyi / Constandse, Rodger M / Damron, Weston / Dawood, Mariam / DeBuono, Nicole / Dimalanta, John D / Edoli, Laure / Elango, Keerthana / Faustino, Nikka / Feng, Chao / Ferrari, Matthew / Frankie, Keith / Fries, Adam / Galloway, Anne / Gavrila, Vlad / Gemmen, Gregory J / Ghadiali, James / Ghorbani, Arash / Goddard, Logan A / Guetter, Adriana Roginski / Hendricks, Garren L / Hentschel, Jendrik / Honigfort, Daniel J / Hsieh, Yun-Ting / Hwang Fu, Yu-Hsien / Im, Scott K / Jin, Chaoyi / Kabu, Shradha / Kincade, Daniel E / Levy, Shawn / Li, Yu / Liang, Vincent K / Light, William H / Lipsher, Jonathan B / Liu, Tsung-Li / Long, Grace / Ma, Rui / Mailloux, John M / Mandla, Kyle A / Martinez, Anyssa R / Mass, Max / McKean, Daniel T / Meron, Michael / Miller, Edmund A / Moh, Celyne S / Moore, Rachel K / Moreno, Juan / Neysmith, Jordan M / Niman, Cassandra S / Nunez, Jesus M / Ojeda, Micah T / Ortiz, Sara Espinosa / Owens, Jenna / Piland, Geoffrey / Proctor, Daniel J / Purba, Josua B / Ray, Michael / Rong, Daisong / Saade, Virginia M / Saha, Sanchari / Tomas, Gustav Santo / Scheidler, Nicholas / Sirajudeen, Luqmanal H / Snow, Samantha / Stengel, Gudrun / Stinson, Ryan / Stone, Michael J / Sundseth, Keoni J / Thai, Eileen / Thompson, Connor J / Tjioe, Marco / Trejo, Christy L / Trieger, Greg / Truong, Diane Ni / Tse, Ben / Voiles, Benjamin / Vuong, Henry / Wong, Jennifer C / Wu, Chiung-Ting / Yu, Hua / Yu, Yingxian / Yu, Ming / Zhang, Xi / Zhao, Da / Zheng, Genhua / He, Molly / Previte, Michael

    Nature biotechnology

    2023  Volume 42, Issue 1, Page(s) 132–138

    Abstract: We present avidity sequencing, a sequencing chemistry that separately optimizes the processes of stepping along a DNA template and that of identifying each nucleotide within the template. Nucleotide identification uses multivalent nucleotide ligands on ... ...

    Abstract We present avidity sequencing, a sequencing chemistry that separately optimizes the processes of stepping along a DNA template and that of identifying each nucleotide within the template. Nucleotide identification uses multivalent nucleotide ligands on dye-labeled cores to form polymerase-polymer-nucleotide complexes bound to clonal copies of DNA targets. These polymer-nucleotide substrates, termed avidites, decrease the required concentration of reporting nucleotides from micromolar to nanomolar and yield negligible dissociation rates. Avidity sequencing achieves high accuracy, with 96.2% and 85.4% of base calls having an average of one error per 1,000 and 10,000 base pairs, respectively. We show that the average error rate of avidity sequencing remained stable following a long homopolymer.
    MeSH term(s) Nucleotides/genetics ; Nucleotides/chemistry ; DNA/genetics ; DNA/chemistry ; DNA Replication ; Base Pairing ; Polymers
    Chemical Substances Nucleotides ; DNA (9007-49-2) ; Polymers
    Language English
    Publishing date 2023-05-25
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1311932-1
    ISSN 1546-1696 ; 1087-0156
    ISSN (online) 1546-1696
    ISSN 1087-0156
    DOI 10.1038/s41587-023-01750-7
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Achievement of Target Gain Larger than Unity in an Inertial Fusion Experiment.

    Abu-Shawareb, H / Acree, R / Adams, P / Adams, J / Addis, B / Aden, R / Adrian, P / Afeyan, B B / Aggleton, M / Aghaian, L / Aguirre, A / Aikens, D / Akre, J / Albert, F / Albrecht, M / Albright, B J / Albritton, J / Alcala, J / Alday, C /
    Alessi, D A / Alexander, N / Alfonso, J / Alfonso, N / Alger, E / Ali, S J / Ali, Z A / Allen, A / Alley, W E / Amala, P / Amendt, P A / Amick, P / Ammula, S / Amorin, C / Ampleford, D J / Anderson, R W / Anklam, T / Antipa, N / Appelbe, B / Aracne-Ruddle, C / Araya, E / Archuleta, T N / Arend, M / Arnold, P / Arnold, T / Arsenlis, A / Asay, J / Atherton, L J / Atkinson, D / Atkinson, R / Auerbach, J M / Austin, B / Auyang, L / Awwal, A A S / Aybar, N / Ayers, J / Ayers, S / Ayers, T / Azevedo, S / Bachmann, B / Back, C A / Bae, J / Bailey, D S / Bailey, J / Baisden, T / Baker, K L / Baldis, H / Barber, D / Barberis, M / Barker, D / Barnes, A / Barnes, C W / Barrios, M A / Barty, C / Bass, I / Batha, S H / Baxamusa, S H / Bazan, G / Beagle, J K / Beale, R / Beck, B R / Beck, J B / Bedzyk, M / Beeler, R G / Behrendt, W / Belk, L / Bell, P / Belyaev, M / Benage, J F / Bennett, G / Benedetti, L R / Benedict, L X / Berger, R L / Bernat, T / Bernstein, L A / Berry, B / Bertolini, L / 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/ Eggert, J H / Ehrlich, B / Ehrmann, P / Elhadj, S / Ellerbee, C / Elliott, N S / Ellison, C L / Elsner, F / Emerich, M / Engelhorn, K / England, T / English, E / Epperson, P / Epstein, R / Erbert, G / Erickson, M A / Erskine, D J / Erlandson, A / Espinosa, R J / Estes, C / Estabrook, K G / Evans, S / Fabyan, A / Fair, J / Fallejo, R / Farmer, N / Farmer, W A / Farrell, M / Fatherley, V E / Fedorov, M / Feigenbaum, E / Fehrenbach, T / Feit, M / Felker, B / Ferguson, W / Fernandez, J C / Fernandez-Panella, A / Fess, S / Field, J E / Filip, C V / Fincke, J R / Finn, T / Finnegan, S M / Finucane, R G / Fischer, M / Fisher, A / Fisher, J / Fishler, B / Fittinghoff, D / Fitzsimmons, P / Flegel, M / Flippo, K A / Florio, J / Folta, J / Folta, P / Foreman, L R / Forrest, C / Forsman, A / Fooks, J / Foord, M / Fortner, R / Fournier, K / Fratanduono, D E / Frazier, N / Frazier, T / Frederick, C / Freeman, M S / Frenje, J / Frey, D / Frieders, G / Friedrich, S / Froula, D H / Fry, J / Fuller, T 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    Physical review letters

    2024  Volume 132, Issue 6, Page(s) 65102

    Abstract: On December 5, 2022, an indirect drive fusion implosion on the National Ignition Facility (NIF) achieved a target gain G_{target} of 1.5. This is the first laboratory demonstration of exceeding "scientific breakeven" (or G_{target}>1) where 2.05 MJ of ... ...

    Abstract On December 5, 2022, an indirect drive fusion implosion on the National Ignition Facility (NIF) achieved a target gain G_{target} of 1.5. This is the first laboratory demonstration of exceeding "scientific breakeven" (or G_{target}>1) where 2.05 MJ of 351 nm laser light produced 3.1 MJ of total fusion yield, a result which significantly exceeds the Lawson criterion for fusion ignition as reported in a previous NIF implosion [H. Abu-Shawareb et al. (Indirect Drive ICF Collaboration), Phys. Rev. Lett. 129, 075001 (2022)PRLTAO0031-900710.1103/PhysRevLett.129.075001]. This achievement is the culmination of more than five decades of research and gives proof that laboratory fusion, based on fundamental physics principles, is possible. This Letter reports on the target, laser, design, and experimental advancements that led to this result.
    Language English
    Publishing date 2024-02-23
    Publishing country United States
    Document type Journal Article
    ZDB-ID 208853-8
    ISSN 1079-7114 ; 0031-9007
    ISSN (online) 1079-7114
    ISSN 0031-9007
    DOI 10.1103/PhysRevLett.132.065102
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Lawson Criterion for Ignition Exceeded in an Inertial Fusion Experiment.

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    Physical review letters

    2021  Volume 129, Issue 7, Page(s) 75001

    Abstract: For more than half a century, researchers around the world have been engaged in attempts to achieve fusion ignition as a proof of principle of various fusion concepts. Following the Lawson criterion, an ignited plasma is one where the fusion heating ... ...

    Abstract For more than half a century, researchers around the world have been engaged in attempts to achieve fusion ignition as a proof of principle of various fusion concepts. Following the Lawson criterion, an ignited plasma is one where the fusion heating power is high enough to overcome all the physical processes that cool the fusion plasma, creating a positive thermodynamic feedback loop with rapidly increasing temperature. In inertially confined fusion, ignition is a state where the fusion plasma can begin "burn propagation" into surrounding cold fuel, enabling the possibility of high energy gain. While "scientific breakeven" (i.e., unity target gain) has not yet been achieved (here target gain is 0.72, 1.37 MJ of fusion for 1.92 MJ of laser energy), this Letter reports the first controlled fusion experiment, using laser indirect drive, on the National Ignition Facility to produce capsule gain (here 5.8) and reach ignition by nine different formulations of the Lawson criterion.
    Language English
    Publishing date 2021-03-18
    Publishing country United States
    Document type Journal Article
    ZDB-ID 208853-8
    ISSN 1079-7114 ; 0031-9007
    ISSN (online) 1079-7114
    ISSN 0031-9007
    DOI 10.1103/PhysRevLett.129.075001
    Database MEDical Literature Analysis and Retrieval System OnLINE

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