LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Back to previous search Search history
Advanced search

Your last searches

  1. AU="Calleros Basilio, Laura"
  2. AU="Mohamad, Okbah"
  3. AU="Holy, Jon"
  4. AU="Farber, Mark O"
  5. AU="Gal, Adiv"
  6. AU="Badenhorst, Marelise"
  7. AU="Pachner, Andrew R"
  8. AU="Colón-Ramos, Daniel A" AU="Colón-Ramos, Daniel A"
  9. AU="Modzelewska, Dominika"
  10. AU="Boethig, Dietmar"
  11. AU="Jurinović, Luka"
  12. AU="Yashila Patel"
  13. AU="Zheng, Lei" AU="Zheng, Lei"
  14. AU="Seah, Penelope M Y"
  15. AU="Marianna Milano"
  16. AU="London, Kevin"
  17. AU=Pain Debkumar
  18. AU="Snider, Elizabeth"
  19. AU="Klein, J R"
  20. AU="Deslandes, K."
  21. AU="Xie, Dandan"
  22. AU=Ward Helen
  23. AU="Grace Deukmedjian"
  24. AU="Lindeman, Neal"
  25. AU="Okoli, Mary"
  26. AU="Mathew, Philip"
  27. AU=Staros Eric B

Search results

Result 1 - 2 of total 2

Search options

  1. Article ; Online: Redox Metabolism and Vascular Calcification in Chronic Kidney Disease.

    Carrillo-López, Natalia / Panizo, Sara / Martín-Carro, Beatriz / Mayo Barrallo, Juan Carlos / Román-García, Pablo / García-Castro, Raúl / Fernández-Gómez, Jesús María / Hevia-Suárez, Miguel Ángel / Martín-Vírgala, Julia / Fernández-Villabrille, Sara / Martínez-Arias, Laura / Vázquez, Sara Barrio / Calleros Basilio, Laura / Naves-Díaz, Manuel / Cannata-Andía, Jorge Benito / Quirós-González, Isabel / Alonso-Montes, Cristina / Fernández-Martín, José Luis

    Biomolecules

    2023  Volume 13, Issue 9

    Abstract: Vascular calcification (VC) is a common complication in patients with chronic kidney disease which increases their mortality. Although oxidative stress is involved in the onset and progression of this disorder, the specific role of some of the main redox ...

    Abstract Vascular calcification (VC) is a common complication in patients with chronic kidney disease which increases their mortality. Although oxidative stress is involved in the onset and progression of this disorder, the specific role of some of the main redox regulators, such as catalase, the main scavenger of H
    MeSH term(s) Humans ; Animals ; Rats ; Catalase ; Core Binding Factor Alpha 1 Subunit/genetics ; Hydrogen Peroxide ; Oxidation-Reduction ; Vascular Calcification ; Renal Insufficiency, Chronic
    Chemical Substances Catalase (EC 1.11.1.6) ; Core Binding Factor Alpha 1 Subunit ; Hydrogen Peroxide (BBX060AN9V)
    Language English
    Publishing date 2023-09-20
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2701262-1
    ISSN 2218-273X ; 2218-273X
    ISSN (online) 2218-273X
    ISSN 2218-273X
    DOI 10.3390/biom13091419
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  2. Article ; Online: Quality Assurance of Samples and Processes in the Spanish Renal Research Network (REDinREN) Biobank.

    Calleros-Basilio, Laura / Cortés, María Alicia / García-Jerez, Andrea / Luengo-Rodríguez, Alicia / Orozco-Agudo, Ana / Valdivielso, José Manuel / Rodríguez-Puyol, Diego / Rodríguez-Puyol, Manuel

    Biopreservation and biobanking

    2016  Volume 14, Issue 6, Page(s) 499–510

    Abstract: Background: Biobanks are useful platforms to build bridges between basic, translational, and clinical research and clinical care. They are repositories of high-quality human biological samples ideal for evaluating their histological characteristics and ... ...

    Abstract Background: Biobanks are useful platforms to build bridges between basic, translational, and clinical research and clinical care. They are repositories of high-quality human biological samples ideal for evaluating their histological characteristics and also their genome, transcriptome, and proteome. The Spanish Renal Research Network Biobank contains more than 76,500 well-preserved frozen samples of a wide variety of kidney diseases, collected from 5450 patients seen by over 70 nephrology services throughout the Spanish territory.
    Objective: To determine and to report the results of the quality control of samples and processes conducted in our biobank, implemented in accordance with the requirements of the ISO 9001:2008 international standard.
    Study design: Two types of quality controls were performed: (1) systematic, that is, measurement of viable peripheral blood mononuclear cells (PBMCs) obtained and purity of nucleic acids and (2) ad-hoc, that is, viability of thawed PBMC, DNA extraction process reproducibility, and the integrity and functionality of nucleic acids, implemented on a routine basis.
    Methods and results: PBMC isolation by Ficoll yielded reproducible results and its cryopreserved viability was >90%. Acceptable A260/A280 ratios were obtained for the vast majority of the DNA (n = 2328) and RNA (n = 78) samples analyzed. DNA integrity was demonstrated by agarose gels and by β-globulin gene polymerase chain reaction (PCR) amplification of 1327 and 989 bp fragments. DNA of acceptable quality had at least three bands of β-globulin amplified obtained (n = 26/30). RNA integrity number (RIN) determinations obtained RIN numbers ≥7 (n = 87/96). The amplifiability of nucleic acids was confirmed by qPCR and RT-qPCR of β-actin and GAPDH genes. Long storage or delayed processing time did not affect the quality of the samples analyzed. The processes of DNA extraction also yielded reproducible results.
    Conclusions: These results clearly indicate that our PBMC, DNA, and RNA stored samples meet the required quality standards to be used for biomedical research, ensuring their long-term preservation.
    MeSH term(s) Biological Specimen Banks/organization & administration ; Biological Specimen Banks/standards ; Biomedical Research/standards ; Cell Survival ; Cryopreservation/methods ; Humans ; Leukocytes/cytology ; Nephrology ; Nucleic Acids/isolation & purification ; Nucleic Acids/standards ; Spain ; Specimen Handling/standards
    Chemical Substances Nucleic Acids
    Language English
    Publishing date 2016-12
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2593993-2
    ISSN 1947-5543 ; 1947-5535
    ISSN (online) 1947-5543
    ISSN 1947-5535
    DOI 10.1089/bio.2015.0095
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

To top