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  1. Thesis ; Online: Genetic engineering of microalgae for enhanced lipid production

    Muñoz Segovia, Camilo F.

    2021  

    Abstract: The global demand for food, feed and fossil-based chemicals is increasing due to the continuously growing world population. Excessive and unsustainable consumption is causing anthropogenic effects resulting in high carbon emission, pollution and ... ...

    Abstract The global demand for food, feed and fossil-based chemicals is increasing due to the continuously growing world population. Excessive and unsustainable consumption is causing anthropogenic effects resulting in high carbon emission, pollution and depletion of natural resources. Currently, the main renewable feedstocks used as alternative to petrochemical processes are derived from agricultural crops. Higher plants are a great source of oils which can be used in food, feed, paint, lighting and fuel industries. However, cultivation of crops requires vast areas of arable land, fresh water and minerals. Additionally, depletion of petrochemical resources increases the demand for plant-based fuels, creating a direct competition with food and feed production. Therefore, novel renewable and sustainable alternatives are necessary to reduce the current environmental impact of traditional industrial processes.Microalgae are considered as a promising alternative for the production of commodities. They are capable of converting sunlight, water, CO2 and nutrients into biomass and products which attract interest from pharmaceutical, cosmetic, biofuel, food and feed industries. Furthermore, microalgae can reach significantly higher biomass concentrations and product yields per hectare compared to higher plants, they do not require arable lands and some species can be grown on wastewater or seawater.Several microalgal species can accumulate large amounts of fatty acids when cultivated under stress conditions. At a cellular level, lipids such as triacylglycerols (TAGs) and polyunsaturated fatty acids (PUFAs) can serve either as structural components or storage compounds. Therefore, microalgae are a source of valuable oils that can be used directly as nutritional products or as raw material for biofuel production.Although microalgae have the potential to become a feedstock for lipid production some limitations and challenges remain. In order to achieve an economically feasible process, improvement, optimization and redesign of ...
    Keywords Life Science
    Subject code 571
    Language English
    Publisher Wageningen University
    Publishing country nl
    Document type Thesis ; Online
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  2. Article ; Online: Design of chitosan nanocrystals decorated with amino acids and peptides

    Hrapovic, Sabahudin / Martinez-Farina, Camilo F. / Sui, Jessie / Lavertu, Jean-Danick / Hemraz, Usha D.

    Carbohydrate Polymers. 2022 Dec., v. 298 p.120108-

    2022  

    Abstract: Antimicrobial peptides (AMPs) offer a great promise in designing new therapeutics due to their ability to interfere in bacterial growth by penetrating the cell wall. The overuse of antibiotics has resulted into antibiotic-resistant bacteria and AMPs ... ...

    Abstract Antimicrobial peptides (AMPs) offer a great promise in designing new therapeutics due to their ability to interfere in bacterial growth by penetrating the cell wall. The overuse of antibiotics has resulted into antibiotic-resistant bacteria and AMPs could be an alternative to circumvent this resistance. Chitosan nanocrystals (ChsNCs) are rod-shaped polysaccharide-based nanomaterials, formed by deacetylation of seafood waste. They possess primary amino groups on the surface of the nanoparticles which can be as used a scaffold due to the built-in morphology and ease in functionalization. Here, we developed a new methodology to functionalize ChsNCs with amino acids and peptides by using fundamentals of solid phase peptide synthesis. The resulting functionalized rod-shaped nanomaterials were characterized using nuclear magnetic resonance (NMR), dynamic light scattering (DLS), zeta potential measurements and microscopy imaging. This synthetic strategy could be used in designing ChsNC-based nanomaterials to target specific cells by attaching bioactive peptides to the nanomaterial surface.
    Keywords antibiotic resistance ; antimicrobial peptides ; bacterial growth ; cell walls ; chitosan ; microscopy ; nanocrystals ; nanoparticles ; nuclear magnetic resonance spectroscopy ; seafood waste ; therapeutics ; zeta potential ; Chitosan nanocrystals ; Amino acids ; Peptides ; Nanomaterials ; Solid phase synthesis
    Language English
    Dates of publication 2022-12
    Publishing place Elsevier Ltd
    Document type Article ; Online
    ZDB-ID 1501516-6
    ISSN 1879-1344 ; 0144-8617
    ISSN (online) 1879-1344
    ISSN 0144-8617
    DOI 10.1016/j.carbpol.2022.120108
    Database NAL-Catalogue (AGRICOLA)

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  3. Article ; Online: Photolytic or Oxidative Fragmentation of Trityl Diazeniumdiolate (O

    Baeza Cinco, Miguel Á / Kräh, Sabrina / Guzman, Camilo F / Wu, Guang / Hayton, Trevor W

    Inorganic chemistry

    2022  Volume 61, Issue 38, Page(s) 14924–14928

    Abstract: Exposure of [K(18-crown-6)(THF) ...

    Abstract Exposure of [K(18-crown-6)(THF)
    MeSH term(s) Azo Compounds ; Crown Ethers ; Furans ; Ligands ; Nitric Oxide ; Nitrous Oxide ; Oxidative Stress ; Photolysis
    Chemical Substances Azo Compounds ; Crown Ethers ; Furans ; Ligands ; diazeniumdiolate ; Nitric Oxide (31C4KY9ESH) ; 18-crown-6 (63J177NC5B) ; Nitrous Oxide (K50XQU1029)
    Language English
    Publishing date 2022-09-13
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1484438-2
    ISSN 1520-510X ; 0020-1669
    ISSN (online) 1520-510X
    ISSN 0020-1669
    DOI 10.1021/acs.inorgchem.2c01809
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  4. Article ; Online: Design of chitosan nanocrystals decorated with amino acids and peptides.

    Hrapovic, Sabahudin / Martinez-Farina, Camilo F / Sui, Jessie / Lavertu, Jean-Danick / Hemraz, Usha D

    Carbohydrate polymers

    2022  Volume 298, Page(s) 120108

    Abstract: Antimicrobial peptides (AMPs) offer a great promise in designing new therapeutics due to their ability to interfere in bacterial growth by penetrating the cell wall. The overuse of antibiotics has resulted into antibiotic-resistant bacteria and AMPs ... ...

    Abstract Antimicrobial peptides (AMPs) offer a great promise in designing new therapeutics due to their ability to interfere in bacterial growth by penetrating the cell wall. The overuse of antibiotics has resulted into antibiotic-resistant bacteria and AMPs could be an alternative to circumvent this resistance. Chitosan nanocrystals (ChsNCs) are rod-shaped polysaccharide-based nanomaterials, formed by deacetylation of seafood waste. They possess primary amino groups on the surface of the nanoparticles which can be as used a scaffold due to the built-in morphology and ease in functionalization. Here, we developed a new methodology to functionalize ChsNCs with amino acids and peptides by using fundamentals of solid phase peptide synthesis. The resulting functionalized rod-shaped nanomaterials were characterized using nuclear magnetic resonance (NMR), dynamic light scattering (DLS), zeta potential measurements and microscopy imaging. This synthetic strategy could be used in designing ChsNC-based nanomaterials to target specific cells by attaching bioactive peptides to the nanomaterial surface.
    MeSH term(s) Amino Acids ; Anti-Bacterial Agents/chemistry ; Anti-Bacterial Agents/pharmacology ; Chitosan/chemistry ; Nanoparticles ; Peptides/chemistry
    Chemical Substances Amino Acids ; Anti-Bacterial Agents ; Peptides ; Chitosan (9012-76-4)
    Language English
    Publishing date 2022-09-15
    Publishing country England
    Document type Journal Article
    ZDB-ID 1501516-6
    ISSN 1879-1344 ; 0144-8617
    ISSN (online) 1879-1344
    ISSN 0144-8617
    DOI 10.1016/j.carbpol.2022.120108
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  5. Article ; Online: NO and N

    Baeza Cinco, Miguel Á / Chakraborty, Arunavo / Guzman, Camilo F / Kräh, Sabrina / Wu, Guang / Hayton, Trevor W

    Inorganic chemistry

    2023  Volume 62, Issue 12, Page(s) 4847–4852

    Abstract: Reaction of ... ...

    Abstract Reaction of MBr
    Language English
    Publishing date 2023-03-13
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1484438-2
    ISSN 1520-510X ; 0020-1669
    ISSN (online) 1520-510X
    ISSN 0020-1669
    DOI 10.1021/acs.inorgchem.2c04088
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  6. Article ; Online: Analysis and Discrimination of Canadian Honey Using Quantitative NMR and Multivariate Statistical Methods.

    Burton, Ian W / Kompany-Zareh, Mohsen / Haverstock, Sophie / Haché, Jonathan / Martinez-Farina, Camilo F / Wentzell, Peter D / Berrué, Fabrice

    Molecules (Basel, Switzerland)

    2023  Volume 28, Issue 4

    Abstract: To address the growing concern of honey adulteration in Canada and globally, a quantitative NMR method was developed to analyze 424 honey samples collected across Canada as part of two surveys in 2018 and 2019 led by the Canadian Food Inspection Agency. ... ...

    Abstract To address the growing concern of honey adulteration in Canada and globally, a quantitative NMR method was developed to analyze 424 honey samples collected across Canada as part of two surveys in 2018 and 2019 led by the Canadian Food Inspection Agency. Based on a robust and reproducible methodology, NMR data were recorded in triplicate on a 700 MHz NMR spectrometer equipped with a cryoprobe, and the data analysis led to the identification and quantification of 33 compounds characteristic of the chemical composition of honey. The high proportion of Canadian honey in the library provided a unique opportunity to apply multivariate statistical methods including PCA, PLS-DA, and SIMCA in order to differentiate Canadian samples from the rest of the world. Through satisfactory model validation, both PLS-DA as a discriminant modeling technique and SIMCA as a class modeling method proved to be reliable at differentiating Canadian honey from a diverse set of honeys with various countries of origins and floral types. The replacement method of optimization was successfully applied for variable selection, and trigonelline, proline, and ethanol at a lower extent were identified as potential chemical markers for the discrimination of Canadian and non-Canadian honeys.
    MeSH term(s) Honey/analysis ; Magnetic Resonance Spectroscopy/methods ; Magnetic Resonance Imaging ; Proline ; Canada ; Multivariate Analysis
    Chemical Substances Proline (9DLQ4CIU6V)
    Language English
    Publishing date 2023-02-09
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 1413402-0
    ISSN 1420-3049 ; 1431-5165 ; 1420-3049
    ISSN (online) 1420-3049
    ISSN 1431-5165 ; 1420-3049
    DOI 10.3390/molecules28041656
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    Zs.MO 81: Show issues

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  7. Book ; Online: Statistical Properties of the Population of the Galactic Center Filaments II

    Yusef-Zadeh, F. / Arendt, R. G. / Wardle, M. / Boldyrev, S. / Heywood, I. / Cotton, W. / Camilo, F.

    The Spacing Between Filaments

    2022  

    Abstract: We carry out a population study of magnetized radio filaments in the Galactic center using MeerKAT data by focusing on the spacing between the filaments that are grouped. The morphology of a sample of 43 groupings containing 174 magnetized radio ... ...

    Abstract We carry out a population study of magnetized radio filaments in the Galactic center using MeerKAT data by focusing on the spacing between the filaments that are grouped. The morphology of a sample of 43 groupings containing 174 magnetized radio filaments are presented. Many grouped filaments show harp-like, fragmented cometary tail-like, or loop-like structures in contrast to many straight filaments running mainly perpendicular to the Galactic plane. There are many striking examples of a single filament splitting into two prongs at a junction, suggestive of a flow of plasma along the filaments. Spatial variations in spectral index, brightness, bending and sharpening along the filaments indicate that they are evolving on a 10^{5-6}-year time scale. The mean spacings between parallel filaments in a given grouping peaks at $\sim16''$. We argue by modeling that the filaments in a grouping all lie on the same plane and that the groupings are isotropically oriented in 3D space. One candidate for the origin of filamentation is interaction with an obstacle, which could be a compact radio source, before a filament splits and bends into multiple filaments. In this picture, the obstacle or sets the length scale of the separation between the filaments. Another possibility is synchrotron cooling instability occurring in cometary tails formed as a result of the interaction of cosmic-ray driven Galactic center outflow with obstacles such as stellar winds. In this picture, the mean spacing and the mean width of the filaments are expected to be a fraction of a parsec, consistent with observed spacing.

    Comment: 53 pages, 32 figures, MNRS (in press)
    Keywords Astrophysics - High Energy Astrophysical Phenomena
    Subject code 612
    Publishing date 2022-06-21
    Publishing country us
    Document type Book ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  8. Book ; Online: Modeling of the nonlinear flame response of a Bunsen-type flame via multi-layer perceptron

    Tathawadekar, Nilam / Doan, Nguyen Anh Khoa / Silva, Camilo F. / Thuerey, Nils

    2022  

    Abstract: This paper demonstrates the ability of neural networks to reliably learn the nonlinear flame response of a laminar premixed flame, while carrying out only one unsteady CFD simulation. The system is excited with a broadband, low-pass filtered velocity ... ...

    Abstract This paper demonstrates the ability of neural networks to reliably learn the nonlinear flame response of a laminar premixed flame, while carrying out only one unsteady CFD simulation. The system is excited with a broadband, low-pass filtered velocity signal that exhibits a uniform distribution of amplitudes within a predetermined range. The obtained time series of flow velocity upstream of the flame and heat release rate fluctuations are used to train the nonlinear model using a multi-layer perceptron. Several models with varying hyperparameters are trained and the dropout strategy is used as regularizer to avoid overfitting. The best performing model is subsequently used to compute the flame describing function (FDF) using mono-frequent excitations. In addition to accurately predicting the FDF, the trained neural network model also captures the presence of higher harmonics in the flame response. As a result, when coupled with an acoustic solver, the obtained neural network model is better suited than a classical FDF model to predict limit cycle oscillations characterized by more than one frequency. The latter is demonstrated in the final part of the present study. We show that the RMS value of the predicted acoustic oscillations together with the associated dominant frequencies are in excellent agreement with CFD reference data.
    Keywords Physics - Fluid Dynamics
    Subject code 532 ; 621
    Publishing date 2022-04-11
    Publishing country us
    Document type Book ; Online
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  9. Article ; Online: Quantitative NMR Methodology for the Authentication of Roasted Coffee and Prediction of Blends.

    Burton, Ian W / Martinez Farina, Camilo F / Ragupathy, Subramanyam / Arunachalam, Thirugnanasambandam / Newmaster, Steve / Berrué, Fabrice

    Journal of agricultural and food chemistry

    2020  Volume 68, Issue 49, Page(s) 14643–14651

    Abstract: In response to the need from the food industry for new analytical solutions, a fit-for-purpose ... ...

    Abstract In response to the need from the food industry for new analytical solutions, a fit-for-purpose quantitative
    MeSH term(s) Alkaloids/analysis ; Caffeine/analysis ; Coffea/chemistry ; Coffea/classification ; Coffee/chemistry ; Cooking ; Discriminant Analysis ; Diterpenes/analysis ; Food Contamination/analysis ; Magnetic Resonance Spectroscopy/methods ; Seeds/chemistry ; Seeds/classification
    Chemical Substances 16-O-methylcafestol ; Alkaloids ; Coffee ; Diterpenes ; Caffeine (3G6A5W338E) ; trigonelline (3NQ9N60I00) ; kahweol (6894-43-5) ; cafestol (AC465T6Q6W)
    Language English
    Publishing date 2020-11-30
    Publishing country United States
    Document type Evaluation Study ; Journal Article
    ZDB-ID 241619-0
    ISSN 1520-5118 ; 0021-8561
    ISSN (online) 1520-5118
    ISSN 0021-8561
    DOI 10.1021/acs.jafc.0c06239
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1172: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  10. Article ; Online: Portal thrombosis in antiphospholipid syndrome

    Elkin J. Pelaes Cruz / Yomayra S. Chigne Castro / Wagner B. Rios Pereda / Yran E. Rebaza Castillo / Magaly del Pilar Rodríguez Raza / Brooke M. Rodríguez Cárdenas / Camilo F. Peña-Quispe

    Revista de la Facultad de Medicina Humana, Vol 22, Iss 4, Pp 882-

    Case report

    2022  Volume 887

    Abstract: Antiphospholipid syndrome (APS) is a systemic autoimmune disorder characterized by arterial, venous, or small vessel thrombosis and recurrent early pregnancy loss, fetal loss, or pregnancy morbidity in the context of persistent antiphospholipid ... ...

    Abstract Antiphospholipid syndrome (APS) is a systemic autoimmune disorder characterized by arterial, venous, or small vessel thrombosis and recurrent early pregnancy loss, fetal loss, or pregnancy morbidity in the context of persistent antiphospholipid antibodies. Characterized by the development of multiple thrombotic manifestations, simultaneously or within a short period of time; being portal vein thrombosis (DVT) a rare and serious clinical manifestation and a predictor of poor prognosis.The case of an elderly patient with abdominal pain and portal vein thrombosis associated with APS with antithrombotic treatment and analgesics of favorable evolution is presented.
    Keywords antiphospholipid syndrome ; venous thrombosis ; antibodies ; antiphospholipid ; Medicine ; R ; Medicine (General) ; R5-920
    Language Spanish
    Publishing date 2022-10-01T00:00:00Z
    Publisher Universidad Ricardo Palma
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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