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  1. Article ; Online: Anti-Inflammatory and Histological Analysis of Skin Wound Healing through Topical Application of Mexican Propolis.

    Balderas-Cordero, Daniela / Canales-Alvarez, Octavio / Sánchez-Sánchez, Roberto / Cabrera-Wrooman, Alejandro / Canales-Martinez, Maria Margarita / Rodriguez-Monroy, Marco Aurelio

    International journal of molecular sciences

    2023  Volume 24, Issue 14

    Abstract: Skin wound healing is a complex biochemical process of tissue repair and remodeling in response to injury. Currently, the drugs used to improve the healing process are inaccessible to the population, are costly, and have side effects, making the search ... ...

    Abstract Skin wound healing is a complex biochemical process of tissue repair and remodeling in response to injury. Currently, the drugs used to improve the healing process are inaccessible to the population, are costly, and have side effects, making the search for new treatment alternatives necessary. Propolis is a natural product produced by bees that is widely recognized and used in folk medicine for its multiple biomedical activities. However, therapeutic information regarding Mexican propolis is limited. This study aimed to evaluate the wound-healing effect of the Chihuahua ethanolic extract of propolis (ChEEP). Macroscopic and histological analyses were performed using a mouse wound-healing model. The topic acute toxicity assay showed that propolis at 10% w/v had no toxic effects. ChEEP has antibacterial activity against the Gram-positive bacteria
    MeSH term(s) Animals ; Propolis/pharmacology ; Propolis/therapeutic use ; Wound Healing ; Anti-Bacterial Agents/therapeutic use ; Disease Models, Animal ; Ethanol/pharmacology ; Anti-Inflammatory Agents/pharmacology
    Chemical Substances Propolis (9009-62-5) ; Anti-Bacterial Agents ; Ethanol (3K9958V90M) ; Anti-Inflammatory Agents
    Language English
    Publishing date 2023-07-23
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms241411831
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Biological Activity of

    Espinosa-Espinosa, Lesslie / Canales-Alvarez, Octavio / Rodríguez-López, Marlene Guadalupe / Flores-Tinajero, César Antonio / Canales-Martinez, Maria Margarita / Rodriguez-Monroy, Marco Aurelio

    International journal of molecular sciences

    2023  Volume 24, Issue 13

    Abstract: Essential oils are composed of terpenes, some of which have properties related to healing. ...

    Abstract Essential oils are composed of terpenes, some of which have properties related to healing.
    MeSH term(s) Mice ; Animals ; Oils, Volatile/pharmacology ; Oils, Volatile/chemistry ; Bursera/chemistry ; Terpenes/pharmacology ; Wound Healing ; Monoterpenes/pharmacology ; Monoterpenes/chemistry ; Anti-Bacterial Agents/pharmacology
    Chemical Substances Oils, Volatile ; Terpenes ; Monoterpenes ; Anti-Bacterial Agents
    Language English
    Publishing date 2023-07-03
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms241311040
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  3. Article ; Online: Effect of Mexican Propolis on Wound Healing in a Murine Model of Diabetes Mellitus.

    Canales-Alvarez, Octavio / Canales-Martinez, Maria Margarita / Dominguez-Verano, Pilar / Balderas-Cordero, Daniela / Madrigal-Bujaidar, Eduardo / Álvarez-González, Isela / Rodriguez-Monroy, Marco Aurelio

    International journal of molecular sciences

    2024  Volume 25, Issue 4

    Abstract: Diabetes mellitus (DM) affects the wound healing process, resulting in impaired healing or aberrant scarring. DM increases reactive oxygen species (ROS) production, fibroblast senescence and angiogenesis abnormalities, causing exacerbated inflammation ... ...

    Abstract Diabetes mellitus (DM) affects the wound healing process, resulting in impaired healing or aberrant scarring. DM increases reactive oxygen species (ROS) production, fibroblast senescence and angiogenesis abnormalities, causing exacerbated inflammation accompanied by low levels of TGF-β and an increase in Matrix metalloproteinases (MMPs). Propolis has been proposed as a healing alternative for diabetic patients because it has antimicrobial, anti-inflammatory, antioxidant and proliferative effects and important properties in the healing process. An ethanolic extract of Chihuahua propolis (ChEEP) was obtained and fractionated, and the fractions were subjected to High-Performance Liquid Chromatography with diode-array (HPLC-DAD), High-Performance Liquid Chromatography-Mass Spectrometry (HPLC-MS) and Gas Chromatography-Mass Spectrometry (GC-MS) analyses and 46 compounds were detected. Deep wounds were made in a murine DM model induced by streptozotocin, and the speed of closure and the wound tensile strength were evaluated by the tensiometric method, which showed that ChEEP had similar activity to Recoveron, improving the speed of healing and increasing the wound tensile strength needed to open the wound again. A histological analysis of the wounds was performed using H&E staining, and when Matrix metalloproteinase 9 (MMP9) and α-actin were quantified by immunohistochemistry, ChEEP was shown to be associated with improved histological healing, as indicated by the reduced MMP9 and α-actin expression. In conclusion, topical ChEEP application enhances wound healing in diabetic mice.
    MeSH term(s) Humans ; Mice ; Animals ; Propolis/pharmacology ; Propolis/therapeutic use ; Matrix Metalloproteinase 9/metabolism ; Diabetes Mellitus, Experimental/drug therapy ; Diabetes Mellitus, Experimental/complications ; Disease Models, Animal ; Actins ; Wound Healing
    Chemical Substances Propolis (9009-62-5) ; Matrix Metalloproteinase 9 (EC 3.4.24.35) ; Actins
    Language English
    Publishing date 2024-02-12
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms25042201
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  4. Article ; Online: Essential oils of Bursera morelensis and Lippia graveolens for the development of a new biopesticides in postharvest control.

    Medina-Romero, Yoli Mariana / Hernandez-Hernandez, Ana Bertha / Rodriguez-Monroy, Marco Aurelio / Canales-Martínez, María Margarita

    Scientific reports

    2021  Volume 11, Issue 1, Page(s) 20135

    Abstract: Fruit and vegetable crops that are not consumed immediately, unlike other agricultural products, require economic and time investments until they reach the final consumers. Synthetic agrochemicals are used to maintain and prolong the storage life of ... ...

    Abstract Fruit and vegetable crops that are not consumed immediately, unlike other agricultural products, require economic and time investments until they reach the final consumers. Synthetic agrochemicals are used to maintain and prolong the storage life of crops and avoid losses caused by phytopathogenic microorganisms. However, the excessive use of synthetic agrochemicals creates health problems and contributes to environmental pollution. To avoid these problems, less toxic and environment-friendly alternatives are sought. One of these alternatives is the application of biopesticides. However, few biopesticides are currently used. In this study, the biopesticide activity of Bursera morelensis and Lippia graveolens essential oils was evaluated. Their antifungal activity has been verified in an in vitro model, and chemical composition has been determined using gas chromatography-mass spectrometry. Their antifungal activity was corroborated in vitro, and their activity as biopesticides was subsequently evaluated in a plant model. In addition, the persistence of these essential oils on the surface of the plant model was determined. Results suggest that both essential oils are promising candidates for producing biopesticides. This is the first study showing that B. morelensis and L. graveolens essential oils work by inhibiting mycelial growth and spore germination and are environment-friendly biopesticides.
    MeSH term(s) Antifungal Agents/pharmacology ; Biological Control Agents/pharmacology ; Bursera/chemistry ; Fusarium/drug effects ; Fusarium/growth & development ; Lippia/chemistry ; Lycopersicon esculentum/drug effects ; Lycopersicon esculentum/growth & development ; Oils, Volatile/pharmacology ; Pesticides/pharmacology ; Plant Extracts/pharmacology ; Plant Oils/pharmacology
    Chemical Substances Antifungal Agents ; Biological Control Agents ; Oils, Volatile ; Pesticides ; Plant Extracts ; Plant Oils
    Language English
    Publishing date 2021-10-11
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-021-99773-0
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: The Wound-Healing Effect of Mango Peel Extract on Incision Wounds in a Murine Model.

    Espinosa-Espinosa, Lesslie / Garduño-Siciliano, Leticia / Rodriguez-Canales, Mario / Hernandez-Portilla, Luis Barbo / Canales-Martinez, Maria Margarita / Rodriguez-Monroy, Marco Aurelio

    Molecules (Basel, Switzerland)

    2022  Volume 27, Issue 1

    Abstract: Mangifera ... ...

    Abstract Mangifera indica
    MeSH term(s) Animals ; Disease Models, Animal ; Flavonoids/chemistry ; Flavonoids/pharmacology ; Male ; Mangifera/chemistry ; Mice ; Plant Extracts/chemistry ; Plant Extracts/pharmacology ; Wound Healing/drug effects ; Wounds and Injuries/drug therapy ; Wounds and Injuries/metabolism ; Wounds and Injuries/microbiology ; Xanthones/chemistry ; Xanthones/pharmacology
    Chemical Substances Flavonoids ; Plant Extracts ; Xanthones ; mangiferin (1M84LD0UMD)
    Language English
    Publishing date 2022-01-01
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 1413402-0
    ISSN 1420-3049 ; 1431-5165 ; 1420-3049
    ISSN (online) 1420-3049
    ISSN 1431-5165 ; 1420-3049
    DOI 10.3390/molecules27010259
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  6. Article ; Online: Essential Oil of

    Salas-Oropeza, Judith / Rodriguez-Monroy, Marco Aurelio / Jimenez-Estrada, Manuel / Perez-Torres, Armando / Castell-Rodriguez, Andres Eliu / Becerril-Millan, Rodolfo / Jarquin-Yanez, Katia / Canales-Martinez, Maria Margarita

    Molecules (Basel, Switzerland)

    2023  Volume 28, Issue 17

    Abstract: Essential oils (EOs) are complex mixtures of volatile natural compounds. We have extensively studied the EO ... ...

    Abstract Essential oils (EOs) are complex mixtures of volatile natural compounds. We have extensively studied the EO of
    MeSH term(s) Bursera ; Oils, Volatile/pharmacology ; Research Design ; Cell Movement ; Fibroblast Growth Factors ; Fibroblasts
    Chemical Substances Oils, Volatile ; Fibroblast Growth Factors (62031-54-3)
    Language English
    Publishing date 2023-08-26
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 1413402-0
    ISSN 1420-3049 ; 1431-5165 ; 1420-3049
    ISSN (online) 1420-3049
    ISSN 1431-5165 ; 1420-3049
    DOI 10.3390/molecules28176258
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  7. Article ; Online: Evaluation of the gastroprotective effects of Chihuahua propolis on indomethacin- induced gastric ulcers in mouse.

    Ruiz-Hurtado, Porfirio Alonso / Garduño-Siciliano, Leticia / Dominguez-Verano, Pilar / Martinez-Galero, Elizdath / Canales-Martinez, Maria Margarita / Rodriguez-Monroy, Marco Aurelio

    Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

    2021  Volume 137, Page(s) 111345

    Abstract: The aim of this work was to evaluate the gastroprotective activity of a Mexican propolis on indomethacin-induced gastric ulcers in a mouse model. The following contents of the ethanolic extract of propolis of Chihuahua (EEPCh) were determined: ... ...

    Abstract The aim of this work was to evaluate the gastroprotective activity of a Mexican propolis on indomethacin-induced gastric ulcers in a mouse model. The following contents of the ethanolic extract of propolis of Chihuahua (EEPCh) were determined: antioxidant activity (SA
    MeSH term(s) Animals ; Anti-Ulcer Agents/chemistry ; Anti-Ulcer Agents/pharmacology ; Anti-Ulcer Agents/therapeutic use ; Antioxidants/analysis ; Cytokines/metabolism ; Dinoprostone/metabolism ; Disease Models, Animal ; Female ; Flavonoids/analysis ; Flavonoids/chemistry ; Gastric Mucosa/drug effects ; Gastric Mucosa/injuries ; Gastric Mucosa/pathology ; Glutathione Peroxidase/drug effects ; Glutathione Peroxidase/metabolism ; Indomethacin/toxicity ; Lipid Peroxidation/drug effects ; Male ; Malondialdehyde/metabolism ; Mice, Inbred ICR ; Omeprazole/pharmacology ; Phenols/analysis ; Phenols/chemistry ; Plant Extracts/chemistry ; Plant Extracts/pharmacology ; Plant Extracts/therapeutic use ; Propolis/chemistry ; Propolis/pharmacology ; Propolis/therapeutic use ; Stomach Ulcer/chemically induced ; Stomach Ulcer/pathology ; Stomach Ulcer/prevention & control ; Superoxide Dismutase/drug effects ; Superoxide Dismutase/metabolism ; Mice
    Chemical Substances Anti-Ulcer Agents ; Antioxidants ; Cytokines ; Flavonoids ; Phenols ; Plant Extracts ; Malondialdehyde (4Y8F71G49Q) ; Propolis (9009-62-5) ; Glutathione Peroxidase (EC 1.11.1.9) ; Superoxide Dismutase (EC 1.15.1.1) ; Dinoprostone (K7Q1JQR04M) ; Omeprazole (KG60484QX9) ; Indomethacin (XXE1CET956)
    Language English
    Publishing date 2021-02-05
    Publishing country France
    Document type Comparative Study ; Journal Article
    ZDB-ID 392415-4
    ISSN 1950-6007 ; 0753-3322 ; 0300-0893
    ISSN (online) 1950-6007
    ISSN 0753-3322 ; 0300-0893
    DOI 10.1016/j.biopha.2021.111345
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  8. Article ; Online: Activity of propolis from Mexico on the proliferation and virulence factors of Candida albicans.

    Rodriguez-Canales, Mario / Medina-Romero, Yoli Mariana / Rodriguez-Monroy, Marco Aurelio / Nava-Solis, Uriel / Bolaños-Cruz, Sandra Isabel / Mendoza-Romero, Maria Jimena / Campos, Jorge E / Hernandez-Hernandez, Ana Bertha / Chirino, Yolanda I / Cruz-Sanchez, Tonatiuh / Garcia-Tovar, Carlos Gerardo / Canales-Martinez, Maria Margarita

    BMC microbiology

    2023  Volume 23, Issue 1, Page(s) 325

    Abstract: Background: This research evaluated the anti-Candida albicans effect of Mexican propolis from Chihuahua. Chemical composition of the ethanolic extract of propolis was determined by GC-MS, HPLC-DAD, and HPLC-MS. The presence of anthraquinone, aromatic ... ...

    Abstract Background: This research evaluated the anti-Candida albicans effect of Mexican propolis from Chihuahua. Chemical composition of the ethanolic extract of propolis was determined by GC-MS, HPLC-DAD, and HPLC-MS. The presence of anthraquinone, aromatic acid, fatty acids, flavonoids, and carbohydrates was revealed.
    Results: The anti-Candida activity of propolis was determined. The inhibitions halos were between 10.0 to 11.8 mm; 25% minimum inhibitory concentration (0.5 mg/ml) was fungistatic, and 50% minimum inhibitory concentration (1.0 mg/ml) was fungicidal. The effect of propolis on the capability of C. albicans to change its morphology was evaluated. 25% minimum inhibitory concentration inhibited to 50% of germ tube formation. Staining with calcofluor-white and propidium iodide was performed, showing that the propolis affected the integrity of the cell membrane. INT1 gene expression was evaluated by qRT-PCR. Propolis significantly inhibited the expression of the INT1 gene encodes an adhesin (Int1p). Chihuahua propolis extract inhibited the proliferation of Candida albicans, the development of the germ tube, and the synthesis of adhesin INT1.
    Conclusions: Given the properties demonstrated for Chihuahua propolis, we propose that it is a candidate to be considered as an ideal antifungal agent to help treat this infection since it would not have the toxic effects of conventional antifungals.
    MeSH term(s) Candida albicans ; Propolis/pharmacology ; Propolis/chemistry ; Virulence Factors ; Mexico ; Antifungal Agents/pharmacology ; Microbial Sensitivity Tests ; Plant Extracts/pharmacology ; Cell Proliferation
    Chemical Substances Propolis (9009-62-5) ; Virulence Factors ; Antifungal Agents ; Plant Extracts
    Language English
    Publishing date 2023-11-04
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2041505-9
    ISSN 1471-2180 ; 1471-2180
    ISSN (online) 1471-2180
    ISSN 1471-2180
    DOI 10.1186/s12866-023-03064-9
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  9. Article ; Online: Effect of the Essential Oils of

    Medina-Romero, Yoli Mariana / Rodriguez-Canales, Mario / Rodriguez-Monroy, Marco Aurelio / Hernandez-Hernandez, Ana Bertha / Delgado-Buenrostro, Norma Laura / Chirino, Yolanda I / Cruz-Sanchez, Tonatiuh / Garcia-Tovar, Carlos Gerardo / Canales-Martinez, Maria Margarita

    Journal of fungi (Basel, Switzerland)

    2022  Volume 8, Issue 6

    Abstract: ... The ... ...

    Abstract The genus
    Language English
    Publishing date 2022-06-09
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2784229-0
    ISSN 2309-608X ; 2309-608X
    ISSN (online) 2309-608X
    ISSN 2309-608X
    DOI 10.3390/jof8060617
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  10. Article ; Online: Antimicrobial activity of the methanolic leaf extract of Prosopis laevigata.

    Nava-Solis, Uriel / Rodriguez-Canales, Mario / Hernandez-Hernandez, Ana Bertha / Velasco-Melgoza, David Arturo / Moreno-Guzman, Brenda Paola / Rodriguez-Monroy, Marco Aurelio / Canales-Martinez, María Margarita

    Scientific reports

    2022  Volume 12, Issue 1, Page(s) 20807

    Abstract: The appearance of antimicrobial-resistant pathogens has highlighted the need to search for new compounds that can effectively combat infectious diseases. A potential source of these compounds are the secondary metabolites of species that have been ... ...

    Abstract The appearance of antimicrobial-resistant pathogens has highlighted the need to search for new compounds that can effectively combat infectious diseases. A potential source of these compounds are the secondary metabolites of species that have been reported as effective traditional treatments of such diseases. Prosopis laevigata is a medicinal plant, and its chemical constituents have shown potential antimicrobial activity. In this study, the antimicrobial activities of the methanolic extract of the leaves of Prosopis laevigata against different bacterial and fungal strains of medical and agronomic interest were investigated in vitro. In addition, the chemical composition of this extract was investigated by HPLC-DAD, GC‒MS, and HPLC‒MS. The methanolic leaf extract contained 67 mg of GAE/g of total phenols (6.7%), 2.6 mg of QE/g of flavonoids (0.26%), and 11.87 mg of AE/g of total alkaloids (1.18%). Phenolic acids and catechol were the compounds identified by HPLC-DAD. The methanolic extract had strong antimicrobial activity, especially against Staphylococcus aureus (MIC = 0.62 mg/mL), Escherichia coli (MIC = 0.62 mg/mL), Candida tropicalis (MIC = 0.08 mg/mL) and Fusarium moniliforme (MIC = 4.62 mg/mL). These results suggest that the extract of P. laevigata leaves could be a source of antimicrobial molecules. However, it is necessary to delve into its chemical composition.
    MeSH term(s) Prosopis ; Methanol ; Hematologic Tests ; Escherichia coli Infections ; Escherichia coli ; Plant Extracts/pharmacology
    Chemical Substances Methanol (Y4S76JWI15) ; Plant Extracts
    Language English
    Publishing date 2022-12-02
    Publishing country England
    Document type Journal Article
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-022-25271-6
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