Article ; Online: Demethylase-independent roles of LSD1 in regulating enhancers and cell fate transition.
2023 Volume 14, Issue 1, Page(s) 4944
Abstract: The major enhancer regulator lysine-specific histone demethylase 1A (LSD1) is required for mammalian embryogenesis and is implicated in human congenital diseases and multiple types of cancer; however, the underlying mechanisms remain enigmatic. Here, we ... ...
Abstract | The major enhancer regulator lysine-specific histone demethylase 1A (LSD1) is required for mammalian embryogenesis and is implicated in human congenital diseases and multiple types of cancer; however, the underlying mechanisms remain enigmatic. Here, we dissect the role of LSD1 and its demethylase activity in gene regulation and cell fate transition. Surprisingly, the catalytic inactivation of LSD1 has a mild impact on gene expression and cellular differentiation whereas the loss of LSD1 protein de-represses enhancers globally and impairs cell fate transition. LSD1 deletion increases H3K27ac levels and P300 occupancy at LSD1-targeted enhancers. The gain of H3K27ac catalyzed by P300/CBP, not the loss of CoREST complex components from chromatin, contributes to the transcription de-repression of LSD1 targets and differentiation defects caused by LSD1 loss. Together, our study demonstrates a demethylase-independent role of LSD1 in regulating enhancers and cell fate transition, providing insight into treating diseases driven by LSD1 mutations and misregulation. |
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MeSH term(s) | Humans ; Animals ; Cell Differentiation ; Regulatory Sequences, Nucleic Acid ; Embryo, Mammalian ; Catalysis ; Histone Demethylases/genetics ; Mammals |
Chemical Substances | Histone Demethylases (EC 1.14.11.-) |
Language | English |
Publishing date | 2023-08-22 |
Publishing country | England |
Document type | Journal Article ; Research Support, N.I.H., Extramural |
ZDB-ID | 2553671-0 |
ISSN | 2041-1723 ; 2041-1723 |
ISSN (online) | 2041-1723 |
ISSN | 2041-1723 |
DOI | 10.1038/s41467-023-40606-1 |
Database | MEDical Literature Analysis and Retrieval System OnLINE |
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