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  1. Article: Predictors of permanent pacemaker implantation in aortic valve diseases after TAVI with vitaFlow liberty system.

    Ju, Changlin / Xie, Xiangrong / Tang, Shengxin / Cao, Shiping

    Frontiers in cardiovascular medicine

    2023  Volume 10, Page(s) 1277528

    Abstract: Introduction: Permanent pacemaker implantation (PPI) is a known complication in patients with aortic stenosis following transcatheter aortic valve implantation (TAVI). However, there is limited research on TAVI for pure aortic regurgitation (PAR), and ... ...

    Abstract Introduction: Permanent pacemaker implantation (PPI) is a known complication in patients with aortic stenosis following transcatheter aortic valve implantation (TAVI). However, there is limited research on TAVI for pure aortic regurgitation (PAR), and more investigation is needed to determine the occurrence of postoperative cardiac conduction block and the need for PPI in this population. Therefore, this retrospective analysis aimed to evaluate the incidence of cardiac conduction block and the necessity of PPI after TAVI in patients with different types of aortic valve disease, including pure aortic stenosis (PAS), aortic stenosis with regurgitation (ASR), and PAR.
    Methods: Clinical data of 100 patients who TAVI were analyzed retrospectively. The incidence of conduction block was assessed, and clinical factors were examined to predict the necessity of PPI.
    Results: Cardiac conduction block was found to be a common complication following TAVI, particularly in patients with PAR. PAR was identified as an independent risk factor for requiring PPI. Additionally, first-degree atrioventricular block emerged as a sensitive predictor for PPI in patients with PAR.
    Discussion: These findings provide valuable insights into the safety and effectiveness of TAVI, which can help enhance patient management and reduce complications.
    Language English
    Publishing date 2023-09-29
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2781496-8
    ISSN 2297-055X
    ISSN 2297-055X
    DOI 10.3389/fcvm.2023.1277528
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Screening and characterization of a β-xylosidase from Bifidobacterium breve K-110 and its application in the biotransformation of the total flavonoids of epimedium to icariin with α-l-rhamnosidase.

    Su, Jingwen / Wu, Tao / Cao, Shiping / Pei, Jianjun / Zhao, Linguo

    Bioorganic chemistry

    2023  Volume 132, Page(s) 106364

    Abstract: Among the flavonoids of epimedium, epimedin B, epimedin C, and icariin are considered to be representative components and their structures are quite similar. Besides sharing the same backbone, the main difference is the sugar groups attached at the ... ...

    Abstract Among the flavonoids of epimedium, epimedin B, epimedin C, and icariin are considered to be representative components and their structures are quite similar. Besides sharing the same backbone, the main difference is the sugar groups attached at the positions of C-3 and C-7. Despite their structural similarities, their potencies differ significantly, and only icariin is currently included in the Chinese Pharmacopoeia as a quality marker (Q-marker) for epimedium flavonoids. Furthermore, icariin has the functions of anti-aging, anti-inflammation, antioxidation, anti-osteoporosis, and ameliorating fibrosis. We used bioinformatics to look for the GH43 family β-xylosidase genes BbXyl from Bifidobacterium breve K-110, which has a length of 1347 bp and codes for 448 amino acids. This will allow us to convert epimedin B and epimedin C into icariin in a specific way. The expression level of recombinant BbXyl in TB medium containing 1 % inulin as carbon source, with an inducer concentration of 0.05 mmol/L and a temperature of 28 °C, was 86.4 U/mL. Previous studies found that the α-l-rhamnosidase BtRha could convert epoetin C to produce icariin, so we combined BbXyl and BtRha to catalyze the conversion of epimedium total flavonoids in vitro and in vivo to obtain the product icariin. Under optimal conditions, in vitro hydrolysis of 5 g/L of total flavonoids of epimedium eventually yielded a concentration of icariin of 678.1 μmol/L. To explore the conversion of total flavonoids of epimedium in vivo. Under the optimal conditions, the yield of icariin reached 97.27 μmol/L when the total flavonoid concentration of epimedium was 1 g/L. This study is the first to screen xylosidases for the targeted conversion of epimedin B to produce icariin, and the first to report that epimedin B and epimedin C in the raw epimedium flavonoids can convert efficiently to icariin by a collaborative of β-xylosidase and α-l-rhamnosidase.
    MeSH term(s) Epimedium/chemistry ; Bifidobacterium breve/metabolism ; Flavonoids/chemistry ; Xylosidases/genetics ; Xylosidases/metabolism ; Biotransformation
    Chemical Substances icariin (VNM47R2QSQ) ; alpha-L-rhamnosidase (EC 3.2.1.40) ; exo-1,4-beta-D-xylosidase (EC 3.2.1.37) ; Flavonoids ; Xylosidases (EC 3.2.1.-)
    Language English
    Publishing date 2023-01-16
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 120080-x
    ISSN 1090-2120 ; 0045-2068
    ISSN (online) 1090-2120
    ISSN 0045-2068
    DOI 10.1016/j.bioorg.2023.106364
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Efficient production of icariin and baohuoside I from Epimedium Folium flavonoids by fungal α-L-rhamnosidase hydrolysing regioselectively the terminal rhamnose of epimedin C.

    Zhang, Shanshan / Lu, Changning / Cao, Shiping / Li, Qi / Wu, Guangwei / Zhao, Linguo

    Biotechnology for biofuels and bioproducts

    2023  Volume 16, Issue 1, Page(s) 107

    Abstract: Industrial application of icariin and baohuoside I has been hindered by the short supply to a great extent. In this work, a novel GH78 α-L-rhamnosidase AmRha catalyzed the bioconversion of low-value epimedin C in crude Epimedium Folium flavonoids (EFs) ... ...

    Abstract Industrial application of icariin and baohuoside I has been hindered by the short supply to a great extent. In this work, a novel GH78 α-L-rhamnosidase AmRha catalyzed the bioconversion of low-value epimedin C in crude Epimedium Folium flavonoids (EFs) to icariin and baohuoside I was developed. Firstly, the high-level expression of AmRha in Komagataella phaffii GS115 attained an enzyme activity of 571.04 U/mL. The purified recombinant AmRha could hydrolyze α-1,2-rhamnoside bond between two rhamnoses (α-Rha(2 → 1)α-Rha) in epimedin C to produce icariin with a molar conversion rate of 92.3%, in vitro. Furtherly, the biotransformation of epimedin C to icariin by the recombinant Komagataella phaffii GS115 cells was also investigated, which elevated the EFs concentration by fivefold. In addition, biotransformation of epimedins A-C and icariin in the raw EFs to baohuoside I was fulfilled by a collaboration of AmRha and β-glucosidase/β-xylosidase Dth3. The results obtained here provide a new insight into the preparation of high-value products icariin and baohuoside I from cheap raw EFs.
    Language English
    Publishing date 2023-06-30
    Publishing country England
    Document type Journal Article
    ISSN 2731-3654
    ISSN (online) 2731-3654
    DOI 10.1186/s13068-023-02348-6
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Efficient production of the glycosylated derivatives of baicalein in engineered Escherichia coli.

    Zhang, Xiaomeng / Xie, Jingcong / Cao, Shiping / Zhang, Haiyan / Pei, Jianjun / Bu, Su / Zhao, Linguo

    Applied microbiology and biotechnology

    2023  Volume 107, Issue 9, Page(s) 2831–2842

    Abstract: Baicalein-7-O-glucoside and baicalein-7-O-rhamnoside have been proven to possess many pharmacological activities and are potential candidate drug leads and herb supplements. However, their further development is largely limited due to low content in host ...

    Abstract Baicalein-7-O-glucoside and baicalein-7-O-rhamnoside have been proven to possess many pharmacological activities and are potential candidate drug leads and herb supplements. However, their further development is largely limited due to low content in host plants. Few studies reported that both bioactive plant components are prepared through the bioconversion of baicalein that is considered as the common biosynthetic precursor of both compounds. Herein, we constructed a series of the engineered whole-cell bioconversion systems in which the deletion of competitive genes and the introduction of exogenous UDP-glucose supply pathway, glucosyltransferase, rhamnosyltransferase, and the UDP-rhamnose synthesis pathway are made. Using these engineered strains, the precursor baicalein is able to be transformed into baicalein-7-O-glucoside and baicalein-7-O-rhamnoside, with high-titer production, respectively. The further optimization of fermentation conditions led to the final production of 568.8 mg/L and 877.0 mg/L for baicalein-7-O-glucoside and baicalein-7-O-rhamnoside, respectively. To the best of our knowledge, it is the highest production in preparation of baicalein-7-O-glucoside from baicalein so far, while the preparation of baicalein-7-O-rhamnoside is the first reported via bioconversion approach. Our study provides a reference for the industrial production of high-value products baicalein-7-O-glucoside and baicalein-7-O-rhamnoside using engineered E. coli. KEY POINTS: • Integrated design for improving the intracellular UDP-glucose pool • High production of rare baicalein glycosides in the engineered E. coli • Baicalein-7-O-glucoside and baicalein-7-O-rhamnoside.
    MeSH term(s) Escherichia coli/genetics ; Escherichia coli/metabolism ; Glycosides/metabolism ; Uridine Diphosphate Glucose/metabolism ; Glucose/metabolism ; Flavonoids/metabolism
    Chemical Substances baicalein (49QAH60606) ; Glycosides ; Uridine Diphosphate Glucose (V50K1D7P4Y) ; Glucose (IY9XDZ35W2) ; Flavonoids
    Language English
    Publishing date 2023-03-17
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 392453-1
    ISSN 1432-0614 ; 0171-1741 ; 0175-7598
    ISSN (online) 1432-0614
    ISSN 0171-1741 ; 0175-7598
    DOI 10.1007/s00253-023-12464-w
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  5. Article: [Serum annexin A2 level is significantly elevated in patients with coronary heart disease].

    Lin, Zixiang / Liu, Dan / Xue, Yazhi / Wu, Tongwei / Zeng, Xiaorong / Guo, Zhigang / Cao, Shiping

    Nan fang yi ke da xue xue bao = Journal of Southern Medical University

    2020  Volume 40, Issue 3, Page(s) 382–387

    Abstract: Objective: To analyze the correlation of annexin A2 with coronary atherosclerotic heart disease (CAD) and the severity of CAD.: Methods: We collected data from a total of 200 inpatients admitted in our department between August, 2017 and August, 2019. ...

    Abstract Objective: To analyze the correlation of annexin A2 with coronary atherosclerotic heart disease (CAD) and the severity of CAD.
    Methods: We collected data from a total of 200 inpatients admitted in our department between August, 2017 and August, 2019. According to the.
    Results: of coronary angiography, the patients were divided into CAD group (
    Conclusions: Annexin A2 is significantly elevated in patients with CAD, especially in those with ACS, and can be used as a predictor of clinical instability.
    MeSH term(s) Annexin A2/blood ; Aryldialkylphosphatase ; Biomarkers ; Coronary Angiography ; Coronary Artery Disease ; Female ; Humans ; Male ; Severity of Illness Index
    Chemical Substances ANXA2 protein, human ; Annexin A2 ; Biomarkers ; Aryldialkylphosphatase (EC 3.1.8.1) ; PON1 protein, human (EC 3.1.8.1)
    Language Chinese
    Publishing date 2020-05-06
    Publishing country China
    Document type Journal Article
    ZDB-ID 2250951-3
    ISSN 1673-4254
    ISSN 1673-4254
    DOI 10.12122/j.issn.1673-4254.2020.03.16
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Erratum: Therapeutic ultrasound combined with microbubbles improves atherosclerotic plaque stability by selectively destroying the intraplaque neovasculature: Erratum.

    Li, Xinzhong / Guo, Shengcun / Xu, Tong / He, Xiang / Sun, Yili / Chen, Xiaoqiang / Cao, Shiping / Si, Xiaoyun / Liao, Wangjun / Liao, Yulin / Han, Yuan / Bin, Jianping

    Theranostics

    2023  Volume 13, Issue 7, Page(s) 2259–2262

    Abstract: This corrects the article DOI: 10.7150/thno.39553.]. ...

    Abstract [This corrects the article DOI: 10.7150/thno.39553.].
    Language English
    Publishing date 2023-04-17
    Publishing country Australia
    Document type Published Erratum
    ZDB-ID 2592097-2
    ISSN 1838-7640 ; 1838-7640
    ISSN (online) 1838-7640
    ISSN 1838-7640
    DOI 10.7150/thno.81490
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  7. Article ; Online: Diagnostic performance of angiography-derived fractional flow reserve analysis based on bifurcation fractal law for assessing hemodynamic significance of coronary stenosis.

    Liang, Hongbin / Zhang, Qiuxia / Gao, Yiting / Chen, Guojun / Bai, Yujia / Zhang, Yanan / Cui, Kai / Wang, Qiancheng / Cao, Shiping / Hou, Yuqing / Zhang, Heye / Ghista, Dhijnoo N / Liu, Xiujian / Xiu, Jiancheng

    European radiology

    2023  Volume 33, Issue 10, Page(s) 6771–6780

    Abstract: Objectives: Blood flow into the side branch affects the calculation of coronary angiography-derived fractional flow reserve (FFR), called Angio-FFR. Neglecting or improperly compensating for the side branch flow may decrease the diagnostic accuracy of ... ...

    Abstract Objectives: Blood flow into the side branch affects the calculation of coronary angiography-derived fractional flow reserve (FFR), called Angio-FFR. Neglecting or improperly compensating for the side branch flow may decrease the diagnostic accuracy of Angio-FFR. This study aims to evaluate the diagnostic accuracy of a novel Angio-FFR analysis that considers the side branch flow based on the bifurcation fractal law.
    Methods: A one-dimensional reduced-order model based on the vessel segment was used to perform Angio-FFR analysis. The main epicardial coronary artery was divided into several segments according to the bifurcation nodes. Side branch flow was quantified using the bifurcation fractal law to correct the blood flow in each vessel segment. In order to verify the diagnostic performance of our Angio-FFR analysis, two other computational methods were taken as control groups: (i) FFR_s: FFR calculated by delineating the coronary artery tree to consider side branch flow, (ii) FFR_n: FFR calculated by just delineating the main epicardial coronary artery and neglecting the side branch flow.
    Results: The analysis of 159 vessels from 119 patients showed that our Anio-FFR calculation method had comparable diagnostic accuracy to FFR_s and provided significantly higher diagnostic accuracy than that of FFR_n. In addition, using invasive FFR as a reference, the Pearson correlation coefficients of Angio-FFR and FFRs were 0.92 and 0.91, respectively, while that of FFR_n was only 0.85.
    Conclusions: Our Angio-FFR analysis has demonstrated good diagnostic performance in assessing the hemodynamic significance of coronary stenosis by using the bifurcation fractal law to compensate for side branch flow.
    Clinical relevance statement: Bifurcation fractal law can be used to compensate for side branch flow during the Angio-FFR calculation of the main epicardial vessel. Compensating for side branch flow can improve the ability of Angio-FFR to diagnose stenosis functional severity.
    Key points: • The bifurcation fractal law could accurately estimate the blood flow from the proximal main vessel into the main branch, thus compensating for the side branch flow. • Angiography-derived FFR based on the bifurcation fractal law is feasible to evaluate the target diseased coronary artery without delineating the side branch.
    MeSH term(s) Humans ; Fractional Flow Reserve, Myocardial/physiology ; Fractals ; Coronary Stenosis ; Coronary Angiography/methods ; Hemodynamics ; Coronary Vessels/diagnostic imaging ; Severity of Illness Index ; Predictive Value of Tests ; Coronary Artery Disease
    Language English
    Publishing date 2023-05-03
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 1085366-2
    ISSN 1432-1084 ; 0938-7994 ; 1613-3749
    ISSN (online) 1432-1084
    ISSN 0938-7994 ; 1613-3749
    DOI 10.1007/s00330-023-09682-1
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  8. Article ; Online: LCZ696 (sacubitril/valsartan) inhibits pulmonary hypertension induced right ventricular remodeling by targeting pyruvate dehydrogenase kinase 4.

    Shen, Mengjia / Zheng, Cankun / Chen, Lu / Li, Mingjue / Huang, Xiaoxia / He, Mingyuan / Liu, Chiyu / Lin, Hairuo / Liao, Wangjun / Bin, Jianping / Cao, Shiping / Liao, Yulin

    Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

    2023  Volume 162, Page(s) 114569

    Abstract: Background: Right ventricular (RV) function is a major prognostic factor in patients with cardiopulmonary disease. Effective medical therapies are available for left heart failure, but they are usually less effective or even ineffective in right heart ... ...

    Abstract Background: Right ventricular (RV) function is a major prognostic factor in patients with cardiopulmonary disease. Effective medical therapies are available for left heart failure, but they are usually less effective or even ineffective in right heart failure. Here, we tested the hypothesis that LCZ696 (sacubitril/valsartan) can attenuate pressure overload-induced RV remodeling by inhibiting pyruvate dehydrogenase kinase 4 (PDK4).
    Methods: Adult male C57 mice were subjected to transverse aortic constriction (TAC), pulmonary artery constriction (PAC), or sham surgery. Bioinformatics analysis was used to screen for common differentially expressed genes (DEGs) between TAC and PAC. Chemical compounds targeting DEGs were predicted by molecular docking analysis. Effects of LCZ696 on PAC-induced RV remodeling and the associated PDK4-related mechanisms were investigated.
    Results: We found 60 common DEGs between PAC and TAC, and Pdk4 was one of the downregulated DEGs. From 47 chemical compounds with potential cardiovascular activity and PDK4 protein binding ability, we selected LCZ696 to treat PAC-induced RV remodeling because of its high docking score for binding PDK4. Compared with vehicle-treated PAC mice, LCZ696-treated mice had significantly smaller RV wall thickness and RV diameters, less myocardial fibrosis, lower expression of PDK4 protein, and less phosphorylation of glycogen synthase kinase-3β (p-GSK3β). In PAC mice, overexpression of Pdk4 blocked the inhibitory effect of LCZ696 on RV remodeling, whereas conditional knockout of Pdk4 attenuated PAC-induced RV remodeling.
    Conclusions: Pdk4 is a common therapeutic target for pressure overload-induced left ventricular and RV remodeling, and LCZ696 attenuates RV remodeling by downregulating Pdk4 and inhibiting PDK4/p-GSK3β signal.
    MeSH term(s) Male ; Mice ; Animals ; Hypertension, Pulmonary/drug therapy ; Glycogen Synthase Kinase 3 beta ; Ventricular Remodeling ; Molecular Docking Simulation ; Valsartan/therapeutic use ; Biphenyl Compounds/therapeutic use ; Drug Combinations ; Heart Failure ; Disease Models, Animal
    Chemical Substances sacubitril and valsartan sodium hydrate drug combination (WB8FT61183) ; sacubitril (17ERJ0MKGI) ; pyruvate dehydrogenase kinase 4 (EC 2.7.1.-) ; Glycogen Synthase Kinase 3 beta (EC 2.7.11.1) ; Valsartan (80M03YXJ7I) ; Biphenyl Compounds ; Drug Combinations
    Language English
    Publishing date 2023-03-29
    Publishing country France
    Document type Journal Article
    ZDB-ID 392415-4
    ISSN 1950-6007 ; 0753-3322 ; 0300-0893
    ISSN (online) 1950-6007
    ISSN 0753-3322 ; 0300-0893
    DOI 10.1016/j.biopha.2023.114569
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  9. Article ; Online: Cumulative remnant cholesterol predicts cardiovascular outcomes in elderly patients with atherosclerotic cardiovascular disease.

    Xiao, Zhiwen / Lin, Zhongqiu / Xu, Lin / Xu, Wenlong / Huang, Haoxiang / Wang, Yuegang / Cao, Shiping / Xie, Zhiquan / Liao, Wangjun / Liao, Yulin / Bin, Jianping / Feng, Weijing / Chen, Yanmei

    European journal of preventive cardiology

    2023  Volume 30, Issue 17, Page(s) 1924–1934

    Abstract: Aims: Remnant cholesterol (RC) reportedly mediates residual cardiovascular risk in atherosclerotic cardiovascular diseases (ASCVD). However, few studies have characterized long-term cumulative RC exposure among elderly people. The study aimed to ... ...

    Abstract Aims: Remnant cholesterol (RC) reportedly mediates residual cardiovascular risk in atherosclerotic cardiovascular diseases (ASCVD). However, few studies have characterized long-term cumulative RC exposure among elderly people. The study aimed to evaluate the association between cumulative exposure to RC and incident major adverse cardiovascular events (MACE) by analysing a cohort of elderly patients with ASCVD.
    Methods and results: This retrospective multicentre cohort study enrolled ASCVD participants aged ≥75 years with baseline visits occurring from 2006 to 2012 followed by four in-person visits. Cumulative RC was estimated as the area under the curve using measurements from the first to fourth visits by using 9-year data. The time-weighted average (TWA) RC was expressed as cumulative exposure to RC averaged by years. All outcomes were follow-up from the fourth visit to the year 2021. Outcomes included a composite of MACE (stroke, unstable angina pectoris, myocardial infarction, and cardiac death). We included 4,680 participants (73.1% male, mean age 79.3 ± 2.5 years). The median follow-up duration was 6.1 years (interquartile range: 3.4-6.6 years). In the multivariable model adjusted for traditional cardiovascular risk factors, low-density lipoprotein cholesterol level, and most recent RC level, the hazard ratios for MACE that compared the high and low tertiles of the RC variables were 1.30 [95% confidence interval (CI), 1.16-1.44] for cumulative RC and 1.36 (95% CI, 1.23-1.52) for TWA RC. Consistent significant associations were observed among most propensity score analyses.
    Conclusions: Long-term cumulative RC was independently associated with incident MACE in elderly participants with ASCVD, suggesting that achieving and maintaining optimal RC levels later in life may still improve cardiovascular outcomes.
    MeSH term(s) Aged ; Humans ; Male ; Aged, 80 and over ; Female ; Cardiovascular Diseases/diagnosis ; Cardiovascular Diseases/epidemiology ; Cardiovascular Diseases/complications ; Cohort Studies ; Atherosclerosis/diagnosis ; Atherosclerosis/epidemiology ; Atherosclerosis/complications ; Cholesterol ; Stroke ; Risk Factors
    Chemical Substances Cholesterol (97C5T2UQ7J)
    Language English
    Publishing date 2023-09-12
    Publishing country England
    Document type Multicenter Study ; Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2626011-6
    ISSN 2047-4881 ; 2047-4873
    ISSN (online) 2047-4881
    ISSN 2047-4873
    DOI 10.1093/eurjpc/zwad297
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  10. Article: [Efficacy of combined ultrasound and microbubble treatment for thrombolysis for rescuing ischemic tissues in rats at different time after thrombosis].

    Peng, Xiaohong / Li, Hairui / Chen, Xiaoqiang / Zhong, Jiayuan / Liu, Jian / Cao, Shiping

    Nan fang yi ke da xue xue bao = Journal of Southern Medical University

    2018  Volume 38, Issue 9, Page(s) 1089–1094

    Abstract: Objective: To explore the relationship between the time after thrombosis and the efficacy of combined ultrasound and microbubble treatment for rescuing the ischemic tissues.: Methods: Rat models of thrombosis in the right common iliac artery were ... ...

    Abstract Objective: To explore the relationship between the time after thrombosis and the efficacy of combined ultrasound and microbubble treatment for rescuing the ischemic tissues.
    Methods: Rat models of thrombosis in the right common iliac artery were established and received combined ultrasound and microbubble treatment at 3, 6 and 12 h after thrombosis. The recanalization rate of the right common iliac artery was assessed using both 2-dimensional and Doppler ultrasound. The plateau acoustic intensity (AI) was quantified for estimating the skeletal microvascular blood volume, and skeletal muscle injury markers including myoglobin (Mb) and creatinine kinase (CK) were measured using ELISA. Postmortem TUNEL staining was used to detect the apoptotic rate of skeletal muscle cells in the hind limb of the rats.
    Results: Compared with those in 3 h group, the recanalization rate and AI were significantly lower, and the levels of Mb and CK and the apoptotic rate of the skeletal muscle cells were significantly higher in both 6 h group and 12 h group (
    Conclusions: The efficacy of combined ultrasound and microbubble treatment for rescuing ischemic tissues tends to be attenuated as the time after thrombosis prolongs in rats.
    MeSH term(s) Animals ; Apoptosis ; Combined Modality Therapy/methods ; Contrast Media/therapeutic use ; Hindlimb ; Iliac Artery/diagnostic imaging ; Ischemia/therapy ; Microbubbles/therapeutic use ; Rats ; Salvage Therapy/methods ; Thrombolytic Therapy/methods ; Thrombosis/therapy ; Time Factors ; Ultrasonic Therapy/methods ; Ultrasonography
    Chemical Substances Contrast Media
    Language Chinese
    Publishing date 2018-10-29
    Publishing country China
    Document type Journal Article
    ZDB-ID 2250951-3
    ISSN 1673-4254
    ISSN 1673-4254
    DOI 10.12122/j.issn.1673-4254.2018.09.11
    Database MEDical Literature Analysis and Retrieval System OnLINE

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