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Article: HDAC4 Regulates the Proliferation, Differentiation and Apoptosis of Chicken Skeletal Muscle Satellite Cells

Zhao, Jing / Shen, Xiaoxu / Cao, Xinao / He, Haorong / Han, Shunshun / Chen, Yuqi / Cui, Can / Wei, Yuanhang / Wang, Yan / Li, Diyan / Zhu, Qing / Yin, Huadong

Animals. 2020 Jan. 04, v. 10, no. 1

2020

Abstract: The development of skeletal muscle satellite cells (SMSCs) is a complex process that could be regulated by many genes. Previous studies have shown that Histone Deacetylase 4 (HDAC4) plays a critical role in cell proliferation, differentiation, and ... ...

Abstract	The development of skeletal muscle satellite cells (SMSCs) is a complex process that could be regulated by many genes. Previous studies have shown that Histone Deacetylase 4 (HDAC4) plays a critical role in cell proliferation, differentiation, and apoptosis in mouse. However, the function of HDAC4 in chicken muscle development is still unknown. Given that chicken is a very important meat-producing animal that is also an ideal model to study skeletal muscle development, we explored the functions of HDAC4 in chicken SMSCs after the interference of HDAC4. The results showed that HDAC4 was enriched in embryonic skeletal muscle, and it was highly expressed in embryonic muscle than in postnatal muscles. Meanwhile, knockdown of HDAC4 could significantly inhibit the proliferation and differentiation of chicken SMSCs but had no effect on the apoptosis of SMSCs as observed in a series of experiment conducted in vitro. These results indicated that HDAC4 might play a positive role in chicken skeletal muscle growth and development.
Keywords	apoptosis ; cell proliferation ; chickens ; growth and development ; histone deacetylase ; meat animals ; mice ; models ; muscle development ; muscles ; skeletal muscle
Language	English
Dates of publication	2020-0104
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article
Note	NAL-light
ISSN	2076-2615
DOI	10.3390/ani10010084
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: miR-99a-5p Regulates the Proliferation and Differentiation of Skeletal Muscle Satellite Cells by Targeting MTMR3 in Chicken.

Cao, Xinao / Tang, Shuyue / Du, Fei / Li, Hao / Shen, Xiaoxu / Li, Diyan / Wang, Yan / Zhang, Zhichao / Xia, Lu / Zhu, Qing / Yin, Huadong

Genes

2020 Volume 11, Issue 4

Abstract: Noncoding RNAs, especially microRNAs (miRNAs), have been reported to play important roles during skeletal muscle development and regeneration. Our previous sequencing data revealed that miR-99a-5p is one of the most abundant miRNAs in chicken breast ... ...

Abstract	Noncoding RNAs, especially microRNAs (miRNAs), have been reported to play important roles during skeletal muscle development and regeneration. Our previous sequencing data revealed that miR-99a-5p is one of the most abundant miRNAs in chicken breast muscle. The purpose of this study was to reveal the regulatory mechanism of miR-99a-5p in the proliferation and differentiation of chicken skeletal muscle satellite cells (SMSCs). Through the investigation of cell proliferation activity, cell cycle progression, and 5-ethynyl-29-deoxyuridine (EdU) assay, we found that miR-99a-5p can significantly promote the proliferation of SMSCs. Moreover, we found that miR-99a-5p can inhibit myotube formation by decreasing the expression of muscle cell differentiation marker genes. After miR-99a-5p target gene scanning, we confirmed that miR-99a-5p directly targets the 3' untranslated region (UTR) of myotubularin-related protein 3 (MTMR3) and regulates its expression level during chicken SMSC proliferation and differentiation. We also explored the role of MTMR3 in muscle development and found that its knockdown significantly facilitates the proliferation but represses the differentiation of SMSCs, which is opposite to the effects of miR-99a-5p. Overall, we demonstrated that miR-99a-5p regulates the proliferation and differentiation of SMSCs by targeting MTMR3.
MeSH term(s)	3' Untranslated Regions ; Animals ; Cell Differentiation ; Cell Movement ; Cell Proliferation ; Chickens ; Gene Expression Regulation ; MicroRNAs/genetics ; Muscle Development ; Protein Tyrosine Phosphatases, Non-Receptor/genetics ; Protein Tyrosine Phosphatases, Non-Receptor/metabolism ; Satellite Cells, Skeletal Muscle
Chemical Substances	3' Untranslated Regions ; MicroRNAs ; Protein Tyrosine Phosphatases, Non-Receptor (EC 3.1.3.48)
Language	English
Publishing date	2020-03-29
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2527218-4
ISSN	2073-4425 ; 2073-4425
ISSN (online)	2073-4425
ISSN	2073-4425
DOI	10.3390/genes11040369
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: MiR-148a-3p Regulates Skeletal Muscle Satellite Cell Differentiation and Apoptosis via the PI3K/AKT Signaling Pathway by Targeting Meox2.

Yin, Huadong / He, Haorong / Cao, Xinao / Shen, Xiaoxu / Han, Shunshun / Cui, Can / Zhao, Jing / Wei, Yuanhang / Chen, Yuqi / Xia, Lu / Wang, Yan / Li, Diyan / Zhu, Qing

Frontiers in genetics

2020 Volume 11, Page(s) 512

Abstract: As bioinformatic approaches have been developed, it has been demonstrated that microRNAs (miRNAs) are involved in the formation of muscles and play important roles in regulation of muscle cell proliferation and differentiation. Previously, it has been ... ...

Abstract	As bioinformatic approaches have been developed, it has been demonstrated that microRNAs (miRNAs) are involved in the formation of muscles and play important roles in regulation of muscle cell proliferation and differentiation. Previously, it has been demonstrated that miR-148a-3p is one of the most abundant miRNAs in chicken skeletal muscle. Here, we build on that work and demonstrate that miR-148a-3p is important in the control of differentiation of chicken skeletal muscle satellite cells (SMSCs). Elevated expression of miR-148a-3p significantly promoted differentiation and inhibited apoptosis of SMSCs but did not affect proliferation. Furthermore, it was observed that the mesenchyme homeobox 2 (Meox2) is a target gene of miR-148a-3p and that miR-148a-3p can down-regulate expression of Meox2, which promote differentiation of SMSCs and suppress apoptosis. Furthermore, miR-148a-3p overexpression encouraged activation of the PI3K/AKT signaling pathway, which could be recovered by overexpression of Meox2. Overall, these findings suggest that microRNA-148a-3p is a potent promoter of myogenesis via direct targeting of Meox2 and increase of the PI3K/AKT signaling pathway in chicken SMSCs.
Language	English
Publishing date	2020-06-04
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2606823-0
ISSN	1664-8021
ISSN	1664-8021
DOI	10.3389/fgene.2020.00512
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Circular RNA CircFAM188B Encodes a Protein That Regulates Proliferation and Differentiation of Chicken Skeletal Muscle Satellite Cells.

Yin, Huadong / Shen, Xiaoxu / Zhao, Jing / Cao, Xinao / He, Haorong / Han, Shunshun / Chen, Yuqi / Cui, Can / Wei, Yuanhang / Wang, Yan / Li, Diyan / Zhu, Qing

Frontiers in cell and developmental biology

2020 Volume 8, Page(s) 522588

Abstract: Circular RNAs (circRNAs) are recognized as functional non-coding transcripts; however, emerging evidence has revealed that some synthetic circRNAs generate functional peptides or proteins. Additionally, the diverse biological functions of circRNAs ... ...

Abstract	Circular RNAs (circRNAs) are recognized as functional non-coding transcripts; however, emerging evidence has revealed that some synthetic circRNAs generate functional peptides or proteins. Additionally, the diverse biological functions of circRNAs include acting as miRNA-binding sponges, RNA-binding protein regulators, and protein translation templates. Previously, we found that circular RNA circFAM188B is a stable circular RNA and differentially expressed between broiler chickens and layers during embryonic skeletal muscle development. In this study, we found that circFAM188B exhibited a unique pattern of sharply decreased expression from embryonic day 10 (E10) to Day 35 (D35) after hatching. Our experimental results showed that circFAM188B promotes the proliferation, but inhibits the differentiation of chicken skeletal muscle satellite cells (SMSCs). Bioinformatic analysis revealed circFAM188B contain an opening reading frame (ORF) which translate into circFAM188B-103aa, internal ribosome entry site (IRES) analysis further confirmed the coding potential of circFAM188B. In addition, western blot assay detected a flag tagged circFAM188B-103aa, and several peptides of circFAM188B-103aa were detected by LC-MS/MS analysis. We further verified that the role of circFAM188B-103aa in chicken myogenesis is consistent with that of its parent transcript circFAM188B, which facilitates proliferation, but represses differentiation of chicken SMSC. Taken together, these results suggested that a novel protein circFAM188B-103aa encoded by circFAM188B that promotes the proliferation but inhibits the differentiation of chicken SMSCs.
Language	English
Publishing date	2020-11-06
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2737824-X
ISSN	2296-634X
ISSN	2296-634X
DOI	10.3389/fcell.2020.522588
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: miR-9-5p Inhibits Skeletal Muscle Satellite Cell Proliferation and Differentiation by Targeting IGF2BP3 through the IGF2-PI3K/Akt Signaling Pathway.

Yin, Huadong / He, Haorong / Shen, Xiaoxu / Zhao, Jing / Cao, Xinao / Han, Shunshun / Cui, Can / Chen, Yuqi / Wei, Yuanhang / Xia, Lu / Wang, Yan / Li, Diyan / Zhu, Qing

International journal of molecular sciences

2020 Volume 21, Issue 5

Abstract: MicroRNAs are evolutionarily conserved, small non-coding RNAs that play critical post-transcriptional regulatory roles in skeletal muscle development. We previously found that miR-9-5p is abundantly expressed in chicken skeletal muscle. Here, we ... ...

Abstract	MicroRNAs are evolutionarily conserved, small non-coding RNAs that play critical post-transcriptional regulatory roles in skeletal muscle development. We previously found that miR-9-5p is abundantly expressed in chicken skeletal muscle. Here, we demonstrate a new role for miR-9-5p as a myogenic microRNA that regulates skeletal muscle development. The overexpression of miR-9-5p significantly inhibited the proliferation and differentiation of skeletal muscle satellite cells (SMSCs), whereas miR-9-5p inhibition had the opposite effect. We show that insulin-like growth factor 2 mRNA-binding protein 3 (IGF2BP3) is a target gene of miR-9-5p, using dual-luciferase assays, RT-qPCR, and Western Blotting, and that it promotes proliferation and differentiation of SMSCs. In addition, we found that IGF2BP3 regulates IGF-2 expression, using overexpression and knockdown studies. We show that Akt is activated by IGF2BP3 and is essential for IGF2BP3-induced cell development. Together, our results indicate that miR-9-5p could regulate the proliferation and differentiation of myoblasts by targeting IGF2BP3 through IGF-2 and that this activity results in the activation of the PI3K/Akt signaling pathway in skeletal muscle cells.
MeSH term(s)	Animals ; Base Sequence ; Cell Differentiation/genetics ; Cell Line ; Cell Proliferation/genetics ; Chickens/genetics ; Insulin-Like Growth Factor II/metabolism ; MicroRNAs/genetics ; MicroRNAs/metabolism ; Models, Biological ; Phosphatidylinositol 3-Kinases/metabolism ; Proto-Oncogene Proteins c-akt/metabolism ; RNA-Binding Proteins/metabolism ; Satellite Cells, Skeletal Muscle/cytology ; Satellite Cells, Skeletal Muscle/metabolism ; Signal Transduction
Chemical Substances	MicroRNAs ; RNA-Binding Proteins ; Insulin-Like Growth Factor II (67763-97-7) ; Proto-Oncogene Proteins c-akt (EC 2.7.11.1)
Language	English
Publishing date	2020-02-28
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms21051655
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: HDAC4 Regulates the Proliferation, Differentiation and Apoptosis of Chicken Skeletal Muscle Satellite Cells.

Zhao, Jing / Shen, Xiaoxu / Cao, Xinao / He, Haorong / Han, Shunshun / Chen, Yuqi / Cui, Can / Wei, Yuanhang / Wang, Yan / Li, Diyan / Zhu, Qing / Yin, Huadong

Animals : an open access journal from MDPI

2020 Volume 10, Issue 1

Abstract	The development of skeletal muscle satellite cells (SMSCs) is a complex process that could be regulated by many genes. Previous studies have shown that Histone Deacetylase 4 (HDAC4) plays a critical role in cell proliferation, differentiation, and apoptosis in mouse. However, the function of HDAC4 in chicken muscle development is still unknown. Given that chicken is a very important meat-producing animal that is also an ideal model to study skeletal muscle development, we explored the functions of HDAC4 in chicken SMSCs after the interference of HDAC4. The results showed that HDAC4 was enriched in embryonic skeletal muscle, and it was highly expressed in embryonic muscle than in postnatal muscles. Meanwhile, knockdown of HDAC4 could significantly inhibit the proliferation and differentiation of chicken SMSCs but had no effect on the apoptosis of SMSCs as observed in a series of experiment conducted in vitro. These results indicated that HDAC4 might play a positive role in chicken skeletal muscle growth and development.
Language	English
Publishing date	2020-01-04
Publishing country	Switzerland
Document type	Journal Article
ISSN	2076-2615
ISSN	2076-2615
DOI	10.3390/ani10010084
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: MicroRNA Profiling Reveals an Abundant miR-200a-3p Promotes Skeletal Muscle Satellite Cell Development by Targeting TGF-β2 and Regulating the TGF‑β2/SMAD Signaling Pathway.

Yin, Huadong / He, Haorong / Shen, Xiaoxu / Tang, Shuyue / Zhao, Jing / Cao, Xinao / Han, Shunshun / Cui, Can / Chen, Yuqi / Wei, Yuanhang / Wang, Yan / Li, Diyan / Zhu, Qing

International journal of molecular sciences

2020 Volume 21, Issue 9

Abstract: MicroRNAs (miRNAs) are evolutionarily conserved, small noncoding RNAs that play critical post-transcriptional regulatory roles in skeletal muscle development. Chicken is an optimal model to study skeletal muscle formation because its developmental ... ...

Abstract	MicroRNAs (miRNAs) are evolutionarily conserved, small noncoding RNAs that play critical post-transcriptional regulatory roles in skeletal muscle development. Chicken is an optimal model to study skeletal muscle formation because its developmental anatomy is similar to that of mammals. In this study, we identified potential miRNAs in the breast muscle of broilers and layers at embryonic day 10 (E10), E13, E16, and E19. We detected 1836 miRNAs, 233 of which were differentially expressed between broilers and layers. In particular, miRNA-200a-3p was significantly more highly expressed in broilers than layers at three time points. In vitro experiments showed that miR-200a-3p accelerated differentiation and proliferation of chicken skeletal muscle satellite cells (SMSCs) and inhibited SMSCs apoptosis. The transforming growth factor 2 (
MeSH term(s)	Apoptosis/genetics ; Computational Biology/methods ; Gene Expression Profiling ; Gene Regulatory Networks ; High-Throughput Nucleotide Sequencing ; Humans ; MicroRNAs/genetics ; Molecular Sequence Annotation ; RNA, Messenger/genetics ; Satellite Cells, Skeletal Muscle/metabolism ; Signal Transduction ; Smad Proteins/metabolism ; Transforming Growth Factor beta2/genetics ; Transforming Growth Factor beta2/metabolism
Chemical Substances	MicroRNAs ; RNA, Messenger ; Smad Proteins ; Transforming Growth Factor beta2
Language	English
Publishing date	2020-05-05
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms21093274
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Erratum: Zhao, J.; Shen, X.; Cao, X.; He, H.; Han, S.; Chen, Y.; Cui, C.; Wei, Y.; Wang, Y.; Li, D.; Zhu, Q.; Yin, H. HDAC4 Regulates the Proliferation, Differentiation and Apoptosis of Chicken Skeletal Muscle Satellite Cells.

Zhao, Jing / Shen, Xiaoxu / Cao, Xinao / He, Haorong / Han, Shunshun / Chen, Yuqi / Cui, Can / Wei, Yuanhang / Wang, Yan / Li, Diyan / Zhu, Qing / Yin, Huadong

Animals : an open access journal from MDPI

2020 Volume 10, Issue 12

Abstract: The authors wish to make the following corrections to their paper [ ... ]. ...

Abstract	The authors wish to make the following corrections to their paper [...].
Language	English
Publishing date	2020-12-07
Publishing country	Switzerland
Document type	Published Erratum
ISSN	2076-2615
ISSN	2076-2615
DOI	10.3390/ani10122322
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Circular RNA profiling identified an abundant circular RNA circTMTC1 that inhibits chicken skeletal muscle satellite cell differentiation by sponging miR-128-3p.

Shen, Xiaoxu / Liu, Zihao / Cao, Xinao / He, Haorong / Han, Shunshun / Chen, Yuqi / Cui, Can / Zhao, Jing / Li, Diyan / Wang, Yan / Zhu, Qing / Yin, Huadong

International journal of biological sciences

2019 Volume 15, Issue 10, Page(s) 2265–2281

Abstract: Scope: ...

Abstract	Scope:
MeSH term(s)	Animals ; Cell Differentiation/physiology ; Chickens ; MicroRNAs/genetics ; MicroRNAs/metabolism ; RNA, Circular/genetics ; RNA, Circular/metabolism ; Satellite Cells, Skeletal Muscle/metabolism
Chemical Substances	MicroRNAs ; RNA, Circular
Language	English
Publishing date	2019-08-19
Publishing country	Australia
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2179208-2
ISSN	1449-2288 ; 1449-2288
ISSN (online)	1449-2288
ISSN	1449-2288
DOI	10.7150/ijbs.36412
Database	MEDical Literature Analysis and Retrieval System OnLINE

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