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  1. Article: High Genetic Diversity in Third-Generation Cephalosporin-Resistant

    Carlsen, Laura / Grottker, Matthias / Heim, Malika / Knobling, Birte / Schlauß, Sebastian / Wellbrock, Kai / Knobloch, Johannes K

    Pathogens (Basel, Switzerland)

    2024  Volume 13, Issue 1

    Abstract: The spread of multidrug-resistant bacteria from humans or livestock is a critical issue. However, the epidemiology of resistant pathogens across wastewater pathways is poorly understood. Therefore, we performed a detailed comparison of third-generation ... ...

    Abstract The spread of multidrug-resistant bacteria from humans or livestock is a critical issue. However, the epidemiology of resistant pathogens across wastewater pathways is poorly understood. Therefore, we performed a detailed comparison of third-generation cephalosporin-resistant
    Language English
    Publishing date 2024-01-20
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2695572-6
    ISSN 2076-0817
    ISSN 2076-0817
    DOI 10.3390/pathogens13010090
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Movement building responses to COVID-19: lessons from the JASS mobilisation fund.

    Okech, Awino / Essof, Shereen / Carlsen, Laura

    Economia politica (Bologna, Italy)

    2021  Volume 39, Issue 1, Page(s) 249–269

    Abstract: This article draws on the work of Just Associates (JASS), a feminist movement support organisation that strengthens the leadership and organising capacity of community-based women networks in Southern Africa, Southeast Asia, and Mesoamerica, to transform ...

    Abstract This article draws on the work of Just Associates (JASS), a feminist movement support organisation that strengthens the leadership and organising capacity of community-based women networks in Southern Africa, Southeast Asia, and Mesoamerica, to transform the structures that perpetuate inequality and violence. We analyse qualitative interviews and surveys drawn from recipients of the JASS mobilisation fund (JMF), an innovative financial crisis support mechanism for feminist movements. We argue that localisation strategies deployed by women's networks supported by the JMF in response to COVID-19, challenge dominant humanitarian responses that de-centre feminist movements, local knowledge, and expertise. By accounting for local knowledge generated from long histories of movement building, building collective power, and challenging racialised and gendered responses to humanitarian crises, women's collectives and networks supported through the JMF developed contextually relevant responses that challenge patriarchal structural barriers heightened by COVID-19.
    Language English
    Publishing date 2021-08-28
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2509755-6
    ISSN 1973-820X ; 1120-2890
    ISSN (online) 1973-820X
    ISSN 1120-2890
    DOI 10.1007/s40888-021-00241-9
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: DNA methylation-based age estimation for adults and minors: considering sex-specific differences and non-linear correlations.

    Carlsen, Laura / Holländer, Olivia / Danzer, Moritz Fabian / Vennemann, Marielle / Augustin, Christa

    International journal of legal medicine

    2023  Volume 137, Issue 3, Page(s) 635–643

    Abstract: DNA methylation patterns change during human lifetime; thus, they can be used to estimate an individual's age. It is known, however, that correlation between DNA methylation and aging might not be linear and that the sex might influence the methylation ... ...

    Abstract DNA methylation patterns change during human lifetime; thus, they can be used to estimate an individual's age. It is known, however, that correlation between DNA methylation and aging might not be linear and that the sex might influence the methylation status. In this study, we conducted a comparative evaluation of linear and several non-linear regressions, as well as sex-specific versus unisex models. Buccal swab samples from 230 donors aged 1 to 88 years were analyzed using a minisequencing multiplex array. Samples were divided into a training set (n = 161) and a validation set (n = 69). The training set was used for a sequential replacement regression and a simultaneous 10-fold cross-validation. The resulting model was improved by including a cut-off of 20 years, dividing the younger individuals with non-linear from the older individuals with linear dependence between age and methylation status. Sex-specific models were developed and improved prediction accuracy in females but not in males, which might be explained by a small sample set. We finally established a non-linear, unisex model combining the markers EDARADD, KLF14, ELOVL2, FHL2, C1orf132, and TRIM59. While age- and sex-adjustments did not generally improve the performance of our model, we discuss how other models and large cohorts might benefit from such adjustments. Our model showed a cross-validated MAD and RMSE of 4.680 and 6.436 years in the training set and of 4.695 and 6.602 years in the validation set, respectively. We briefly explain how to apply the model for age prediction.
    MeSH term(s) Male ; Female ; Adult ; Humans ; DNA Methylation ; CpG Islands ; Genetic Markers ; Aging/genetics ; Forensic Genetics/methods ; Tripartite Motif Proteins/genetics ; Intracellular Signaling Peptides and Proteins/genetics
    Chemical Substances Genetic Markers ; TRIM59 protein, human ; Tripartite Motif Proteins ; Intracellular Signaling Peptides and Proteins
    Language English
    Publishing date 2023-02-22
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 1055109-8
    ISSN 1437-1596 ; 0937-9827
    ISSN (online) 1437-1596
    ISSN 0937-9827
    DOI 10.1007/s00414-023-02967-6
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    In stock of ZB MED Cologne/Königswinter

    Un I Zs.312: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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  4. Book ; Online ; Thesis: Langzeitpersistenz und Evolution von multi-resistenten Enterobacterales in abwasserführenden Systemen

    Carlsen, Laura [Verfasser] / Streit, Wolfgang [Akademischer Betreuer] / Knobloch, Johannes [Akademischer Betreuer]

    2023  

    Author's details Laura Carlsen ; Wolfgang Streit, Johannes Knobloch
    Keywords Biowissenschaften, Biologie ; Life Science, Biology
    Subject code sg570
    Language German
    Publisher Staats- und Universitätsbibliothek Hamburg Carl von Ossietzky
    Publishing place Hamburg
    Document type Book ; Online ; Thesis
    Database Digital theses on the web

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  5. Article: Phenotypic Variation in Clinical

    Knobling, Birte / Franke, Gefion / Carlsen, Laura / Belmar Campos, Cristina / Büttner, Henning / Klupp, Eva M / Maurer, Philipp Maximilian / Knobloch, Johannes K

    Microorganisms

    2023  Volume 11, Issue 5

    Abstract: Pigmentation, catalase activity and biofilm formation are virulence factors that cause resistance ... ...

    Abstract Pigmentation, catalase activity and biofilm formation are virulence factors that cause resistance of
    Language English
    Publishing date 2023-05-18
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2720891-6
    ISSN 2076-2607
    ISSN 2076-2607
    DOI 10.3390/microorganisms11051332
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  6. Article ; Online: High burden and diversity of carbapenemase-producing Enterobacterales observed in wastewater of a tertiary care hospital in Germany.

    Carlsen, Laura / Büttner, Henning / Christner, Martin / Franke, Gefion / Indenbirken, Daniela / Knobling, Birte / Lütgehetmann, Marc / Knobloch, Johannes

    International journal of hygiene and environmental health

    2022  Volume 242, Page(s) 113968

    Abstract: Hospitals are one of the main reservoirs of multi-resistant Enterobacterales (MRE). As MRE are resistant to the most frequently used antibiotics, therapy for patients with MRE infections is challenging. It has been previously described that MRE from ... ...

    Abstract Hospitals are one of the main reservoirs of multi-resistant Enterobacterales (MRE). As MRE are resistant to the most frequently used antibiotics, therapy for patients with MRE infections is challenging. It has been previously described that MRE from hospital wastewater can pass into municipal wastewater and even surface water. In this study, we investigated the diversity and epidemiology of MRE in the wastewater of a large tertiary care hospital. Wastewater samples were collected for a four-day period and tested for the presence of Enterobacterales resistant to 3rd gen. cephalosporins. Representative isolates were further characterized by whole genome sequencing. In 120 β-glucuronidase-producing isolates, 68 Escherichia coli and, interestingly, also 52 Citrobacter freundii were identified. In 120 β-glucosidase-producing isolates 45 Serratia marcescens, 34 Klebsiella oxytoca, 32 Enterobacter cloacae and 9 Klebsiella pneumoniae were observed. For all species various MLST sequence types and different clusters of resistance genes were determined, showing a great diversity within the different Enterobacterales, further corroborated by clonal analysis performed by cgMLST. The most prominent clone was wastewater associated E. coli ST635, which accounted for 47.1% of all E. coli isolates. Interestingly, 45.6% of E. coli, 88.5% of C. freundii, 95.6% of S. marcescens, 91.2% of K. oxytoca, 96.9% of E. cloacae and 88.9% of K. pneumoniae isolates carried a carbapenemase gene, indicating a high burden with carbapenemase-producing Enterobacterales. Comparison with clinical isolates from the same hospital displayed few clonal matches. One wastewater isolate of K. pneumoniae was identified to be closely related compared to a clone that had been introduced into the hospital during an outbreak four years earlier. One E. coli isolate was identified as identical to an isolate from a patient, with inpatient stay during the sampling period. The data obtained in this study highlight the problem of antibiotic resistance of Enterobacterales in hospital wastewater. In particular, the clustered occurrence of carbapenemase genes is of great concern and underscores the problem of increasingly scarce antibiotic options against these bacteria.
    MeSH term(s) Anti-Bacterial Agents/pharmacology ; Bacterial Proteins/genetics ; Escherichia coli/genetics ; Humans ; Klebsiella pneumoniae/genetics ; Microbial Sensitivity Tests ; Multilocus Sequence Typing ; Tertiary Care Centers ; Waste Water ; beta-Lactamases
    Chemical Substances Anti-Bacterial Agents ; Bacterial Proteins ; Waste Water ; beta-Lactamases (EC 3.5.2.6) ; carbapenemase (EC 3.5.2.6)
    Language English
    Publishing date 2022-04-04
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2009176-X
    ISSN 1618-131X ; 1438-4639
    ISSN (online) 1618-131X
    ISSN 1438-4639
    DOI 10.1016/j.ijheh.2022.113968
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 5334: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  7. Article ; Online: Long time persistence and evolution of carbapenemase-producing Enterobacterales in the wastewater of a tertiary care hospital in Germany.

    Carlsen, Laura / Büttner, Henning / Christner, Martin / Cordts, Lukas / Franke, Gefion / Hoffmann, Armin / Knobling, Birte / Lütgehetmann, Marc / Nakel, Jacqueline / Werner, Thomas / Knobloch, Johannes K

    Journal of infection and public health

    2023  Volume 16, Issue 8, Page(s) 1142–1148

    Abstract: Background: Worldwide observations revealed increased frequencies of multi-resistant Enterobacterales and resistance genes in hospital wastewater compared to any other type of wastewater. Despite the description of clonal lineages possibly adapted to ... ...

    Abstract Background: Worldwide observations revealed increased frequencies of multi-resistant Enterobacterales and resistance genes in hospital wastewater compared to any other type of wastewater. Despite the description of clonal lineages possibly adapted to hospital wastewater, little is known about long term persistence as well as evolution of these lineages.
    Methods: In this study, wastewater isolates of different Enterobacterales species from a tertiary care hospital were investigated with 2.5 years distance. Whole Genome Sequencing (WGS) and resistance gene identification were performed for E. coli, C. freundii, S. marcescens, K. pneumoniae, K. oxytoca, and E. cloacae isolates (n = 59), isolated in 2022 and compared with strains isolated from the same wastewater pipeline in 2019 (n = 240).
    Results: Individual clonal lineages with highly related isolates could be identified in all species identified more than once in 2022 that appear to persist in the wastewater drainage. A common motif of all persistent clonal lineages was the carriage of mobile genetic elements encoding carbapenemase genes with hints for horizontal gene transfer in persistent clones in this environment observed over the 2.5-year period. Multiple plasmid replicons could be detected in both years. In 2022 isolates bla
    Conclusions: This data indicates that hospital wastewater continuously releases genes encoding carbapenemases to the urban wastewater system. The evolution of the resident clones as well as the reasons for the selection advantage in this specific ecological niche needs to be further investigated in the future.
    MeSH term(s) Humans ; Wastewater ; Escherichia coli ; Tertiary Care Centers ; Bacterial Proteins/genetics ; beta-Lactamases/genetics ; Klebsiella pneumoniae/genetics ; Anti-Bacterial Agents/pharmacology ; Microbial Sensitivity Tests
    Chemical Substances carbapenemase (EC 3.5.2.6) ; Wastewater ; Bacterial Proteins ; beta-Lactamases (EC 3.5.2.6) ; Anti-Bacterial Agents
    Language English
    Publishing date 2023-05-26
    Publishing country England
    Document type Journal Article
    ZDB-ID 2467587-8
    ISSN 1876-035X ; 1876-0341
    ISSN (online) 1876-035X
    ISSN 1876-0341
    DOI 10.1016/j.jiph.2023.05.029
    Database MEDical Literature Analysis and Retrieval System OnLINE

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