LIVIVO - Search results -

Search results

Result 1 - 10 of total 32

Search options

Article ; Online: Mitochondria and the NLRP3 Inflammasome in Alcoholic and Nonalcoholic Steatohepatitis

Sandra Torres / Paula Segalés / Carmen García-Ruiz / José C. Fernández-Checa

Cells, Vol 11, Iss 1475, p

2022 Volume 1475

Abstract: Alcoholic (ASH) and nonalcoholic steatohepatitis (NASH) are advanced stages of fatty liver disease and two of the most prevalent forms of chronic liver disease. ASH and NASH are associated with significant risk of further progression to cirrhosis and ... ...

Abstract	Alcoholic (ASH) and nonalcoholic steatohepatitis (NASH) are advanced stages of fatty liver disease and two of the most prevalent forms of chronic liver disease. ASH and NASH are associated with significant risk of further progression to cirrhosis and hepatocellular carcinoma (HCC), the most common type of liver cancer, and a major cause of cancer-related mortality. Despite extensive research and progress in the last decades to elucidate the mechanisms of the development of ASH and NASH, the pathogenesis of both diseases is still poorly understood. Mitochondrial damage and activation of inflammasome complexes have a role in inducing and sustaining liver damage. Mitochondrial dysfunction produces inflammatory factors that activate the inflammasome complexes. NLRP3 inflammasome (nucleotide-binding oligomerization domain-like receptor protein 3) is a multiprotein complex that activates caspase 1 and the release of pro-inflammatory cytokines, including interleukin-1β (IL-1β) and interleukin-18 (IL-18), and contributes to inflammatory pyroptotic cell death. The present review, which is part of the issue “Mitochondria in Liver Pathobiology”, provides an overview of the role of mitochondrial dysfunction and NLRP3 activation in ASH and NASH.
Keywords	NLRP3 inflammasome ; mitochondria ; alcoholism ; obesity ; steatohepatitis ; Biology (General) ; QH301-705.5
Subject code	610
Language	English
Publishing date	2022-04-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Assessment of Volatile Compound Transference through Firefighter Turnout Gear

María José Aliaño-González / Gemma Montalvo / Carmen García-Ruiz / Marta Ferreiro-González / Miguel Palma

International Journal of Environmental Research and Public Health, Vol 19, Iss 3663, p

2022 Volume 3663

Abstract: There is high concern about the exposure of firefighters to toxic products or carcinogens resulting from combustion during fire interventions. Firefighter turnout gear is designed to protect against immediate fire hazards but not against chemical agents. ...

Abstract	There is high concern about the exposure of firefighters to toxic products or carcinogens resulting from combustion during fire interventions. Firefighter turnout gear is designed to protect against immediate fire hazards but not against chemical agents. Additionally, the decontamination of firefighter personal protective equipment remains unresolved. This study evaluated the feasibility of a screening method based on headspace-gas chromatography-ion mobility spectrometry (HS-GC-IMS) in combination with chemometrics (cluster analysis, principal component analysis, and linear discriminant analysis) for the assessment of the transference of volatile compounds through turnout gear. To achieve this, firefighter turnout gears exposed to two different fire scenes (with different combustion materials) were directly analyzed. We obtained a spectral fingerprint for turnout gears that were both exposed and non-exposed to fire scenes. The results showed that (i): the contamination of the turnout gears is different depending on the type of fire loading; and (ii) it is possible to determine if the turnout gear is free of volatile compounds. Based on the latest results, we concluded that HS-GC-IMS can be applied as a screening technique to assess the quality of turnout gear prior to a new fire intervention.
Keywords	volatile organic compounds (VOCs) ; fire ; occupational risk ; toxicity ; turnout gear ; firefighter ; Medicine ; R
Language	English
Publishing date	2022-03-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Mitochondrial cholesterol

Leire Goicoechea / Laura Conde de la Rosa / Sandra Torres / Carmen García-Ruiz / José C. Fernández-Checa

Redox Biology, Vol 61, Iss , Pp 102643- (2023)

Metabolism and impact on redox biology and disease

2023

Abstract: Cholesterol is a crucial component of membrane bilayers by regulating their structural and functional properties. Cholesterol traffics to different cellular compartments including mitochondria, whose cholesterol content is low compared to other cell ... ...

Abstract	Cholesterol is a crucial component of membrane bilayers by regulating their structural and functional properties. Cholesterol traffics to different cellular compartments including mitochondria, whose cholesterol content is low compared to other cell membranes. Despite the limited availability of cholesterol in the inner mitochondrial membrane (IMM), the metabolism of cholesterol in the IMM plays important physiological roles, acting as the precursor for the synthesis of steroid hormones and neurosteroids in steroidogenic tissues and specific neurons, respectively, or the synthesis of bile acids through an alternative pathway in the liver. Accumulation of cholesterol in mitochondria above physiological levels has a negative impact on mitochondrial function through several mechanisms, including the limitation of crucial antioxidant defenses, such as the glutathione redox cycle, increased generation of reactive oxygen species and consequent oxidative modification of cardiolipin, and defective assembly of respiratory supercomplexes. These adverse consequences of increased mitochondrial cholesterol trafficking trigger the onset of oxidative stress and cell death, and, ultimately, contribute to the development of diverse diseases, including metabolic liver diseases (i.e. fatty liver disease and liver cancer), as well as lysosomal disorders (i.e. Niemann-Pick type C disease) and neurodegenerative diseases (i.e. Alzheimer's disease). In this review, we summarize the metabolism and regulation of mitochondrial cholesterol and its potential impact on liver and neurodegenerative diseases.
Keywords	Mitochondria ; Cholesterol ; ROS ; Metabolism ; Liver disease ; Neurodegeneration ; Medicine (General) ; R5-920 ; Biology (General) ; QH301-705.5
Subject code	610
Language	English
Publishing date	2023-05-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: GST-Perfringolysin O production for the localization and quantification of membrane cholesterol in human and mouse brain and liver

Leire Goicoechea / Fabian Arenas / Fernanda Castro / Susana Nuñez / Sandra Torres / Carmen Garcia-Ruiz / José C. Fernandez-Checa

STAR Protocols, Vol 3, Iss 1, Pp 101068- (2022)

2022

Abstract: Summary: Abnormal cholesterol metabolism is linked to many neurodegenerative disorders. Here, we present a protocol for the production of a recombinant protein consisting of a Glutathione-S-Transferase tag fused with the Perfringolysin O (PFO). The GST- ... ...

Abstract	Summary: Abnormal cholesterol metabolism is linked to many neurodegenerative disorders. Here, we present a protocol for the production of a recombinant protein consisting of a Glutathione-S-Transferase tag fused with the Perfringolysin O (PFO). The GST-PFO tag enables analysis of the localization of cholesterol in subcellular membranes of human and mice brain and liver tissues. We have used this approach for samples from Niemann-Pick type C disease and non-alcoholic steatohepatitis models. The construct may also have applications for the diagnosis of cholesterol-accumulating disorders.For complete details on the use and execution of this protocol, please refer to Kwiatkowska et al. (2014).
Keywords	Cell Biology ; Cell Membrane ; Health Sciences ; Metabolism ; Microscopy ; Model Organisms ; Science (General) ; Q1-390
Language	English
Publishing date	2022-03-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article: Analysis of different materials subjected to open-air explosions in search of explosive traces by Raman microscopy

Zapata, Félix / Carmen García-Ruiz

Forensic science international. 2017 June, v. 275

2017

Abstract: Post-explosion scenes offer such chaos and destruction that evidence recovery and detection of post-blast residues from the explosive in the surrounding materials is highly challenging and difficult. The suitability of materials to retain explosives ... ...

Abstract	Post-explosion scenes offer such chaos and destruction that evidence recovery and detection of post-blast residues from the explosive in the surrounding materials is highly challenging and difficult. The suitability of materials to retain explosives residues and their subsequent analysis has been scarcely investigated. Particularly, the use of explosive mixtures containing inorganic oxidizing salts to make improvised explosive devices (IEDs) is a current security concern due to their wide availability and lax control. In this work, a wide variety of materials such as glass, steel, plywood, plastic bag, brick, cardboard or cotton subjected to open-air explosions were examined using confocal Raman microscopy, aiming to detect the inorganic oxidizing salts contained in explosives as black powder, chloratite, dynamite, ammonium nitrate fuel oil and ammonal. Post-blast residues were detected through microscopic examination of materials surfaces. In general, the more homogeneous and smoother the surface was, the less difficulties and better results in terms of identification were obtained. However, those highly irregular surfaces were the most unsuitable collectors for the posterior identification of explosive traces by Raman microscopy. The findings, difficulties and some recommendations related to the identification of post-blast particles in the different materials studied are thoroughly discussed.
Keywords	ammonium nitrate ; bricks ; cardboard ; collectors ; cotton ; explosions ; explosives ; fuel oils ; glass ; microscopy ; plywood ; Raman imaging ; steel
Language	English
Dates of publication	2017-06
Size	p. 57-64.
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	424042-x
ISSN	1872-6283 ; 0379-0738
ISSN (online)	1872-6283
ISSN	0379-0738
DOI	10.1016/j.forsciint.2017.02.032
Database	NAL-Catalogue (AGRICOLA)

In stock of ZB MED Cologne/Königswinter

Zs.B 1104: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Details ▾
- See ZB MED holdings
- Order with fees

Article: Comparison of different GC-MS configurations for the determination of prevalent drugs and related metabolites

Sáiz, Jorge / Carmen García-Ruiz / Belén Gómara

Analytical methods. 2017 May 18, v. 9, no. 19

2017

Abstract: Cocaine, cannabis, heroin, and other opioids are among the prevalent drugs in Europe. The use of these drugs is demonstrated by the determination of either parent drugs or related metabolites in a variety of biological samples. Various analytical ... ...

Abstract	Cocaine, cannabis, heroin, and other opioids are among the prevalent drugs in Europe. The use of these drugs is demonstrated by the determination of either parent drugs or related metabolites in a variety of biological samples. Various analytical methodologies can be applied for this purpose, all of which might show relevant differences in analytical performance. In this work we used different GC-MS configurations for the quantitation of cocaine, cocaethylene, benzoylecgonine, morphine, and Δ9-tetrahydrocannabinol with the aim of comparing the analytical performance of different GC-MS instruments, different injectors, ion sources, ionization modes, mass analyzers, operating modes, and acquisition modes, in order to find the optimal configuration in terms of sensitivity and precision. Other important factors, such as the derivatization process for GC analysis or the injection mode, were also investigated for the same purpose. A comparative study of different methods used for the calculation of the limits of detection was also performed, in order to compare them in terms of the obtained values and their veracity. Differences found in the results obtained with different configurations showed different limits of detection and different precision. These results allowed us to indicate advantages and limitations, which depended on the configuration of the GC-MS used. Finally, differences up to seven orders of magnitude were found in the LOD values obtained with different methods, some of them being too small to show any measurable peak.
Keywords	cocaine ; derivatization ; detection limit ; gas chromatography-mass spectrometry ; heroin ; injectors ; ionization ; metabolites ; morphine ; narcotics ; tetrahydrocannabinol ; Europe
Language	English
Dates of publication	2017-0518
Size	p. 2897-2908.
Publishing place	The Royal Society of Chemistry
Document type	Article
ZDB-ID	2515210-5
ISSN	1759-9679 ; 1759-9660
ISSN (online)	1759-9679
ISSN	1759-9660
DOI	10.1039/c7ay00813a
Database	NAL-Catalogue (AGRICOLA)

Order via subito

Article: Recent advances in capillary electrophoresis instrumentation for the analysis of explosives

Calcerrada, Matías / Carmen García-Ruiz / Miguel González-Herráez

Trends in analytical chemistry. 2016 Jan., v. 75

2016

Abstract: Capillary electrophoresis (CE) is a well-established analytical separation technique. Owing to its high versatility, major advancements have been made with regard to the instrumental set-ups during the last years. New strategies have been proposed to ... ...

Abstract	Capillary electrophoresis (CE) is a well-established analytical separation technique. Owing to its high versatility, major advancements have been made with regard to the instrumental set-ups during the last years. New strategies have been proposed to develop high-sensitive methods, portable CE or miniaturized devices. These techniques are of great interest in the analysis of explosives, which generally requires a highly selective approach. This review provides a recent perspective (from the beginning of 2008 to March 2015) on the use of CE for the analysis of explosives. First, a general description of explosives is made, emphasizing the role of separation techniques and specifically CE. Next, the most recent works focused on the analysis of explosives by using conventional CE, portable CE and microchip CE are compared and critically discussed. Besides, other emerging techniques for the analysis of explosives are referred and compared to CE results. Finally, future perspectives for the analysis of explosives by CE are proposed.
Keywords	capillary electrophoresis ; explosives ; instrumentation
Language	English
Dates of publication	2016-01
Size	p. 75-85.
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	2014041-1
ISSN	0165-9936
ISSN	0165-9936
DOI	10.1016/j.trac.2015.08.005
Database	NAL-Catalogue (AGRICOLA)

Order via subito

Article: Multi-spectral imaging for the estimation of shooting distances

Zapata, Félix / Carmen García-Ruiz / José Manuel Amigo / María López-López

Forensic science international. 2018 Jan., v. 282

2018

Abstract: Multispectral images of clothing targets shot at seven different distances (from 10 to 220cm) were recorded at 18 specific wavelengths in the 400–1000nm range to visualize the gunshot residue (GSR) pattern. Principal component analysis (PCA) showed that ... ...

Abstract	Multispectral images of clothing targets shot at seven different distances (from 10 to 220cm) were recorded at 18 specific wavelengths in the 400–1000nm range to visualize the gunshot residue (GSR) pattern. Principal component analysis (PCA) showed that the use of violet-blue wavelengths (430, 450 and 470nm) provided the largest contrast between the GSR particles and the white cotton fabric. Then, the correlation between the amount of GSR particles on clothing targets and the shooting distance was studied. By selecting the blue frame of multispectral images (i.e. the blue frame in the red-green-blue (RGB) system which falls at 470nm), the amount of pixels containing GSR particles was accounted based on the intensity of pixels in that frame. Results demonstrated that the number of pixels containing GSR exponentially decreases with the shooting distance from 30 to 220cm following a particular exponential equation. However, the targets shot at the shortest distance (10cm) did not satisfy the above equation, probably due to the noticeable differences of the GSR-pattern of these targets (e.g. high presence of soot). Then, the equation was applied to validation samples to estimate the shooting distances, obtaining results with an error below 10%.
Keywords	cotton fabric ; equations ; multispectral imagery ; principal component analysis ; soot ; wavelengths
Language	English
Dates of publication	2018-01
Size	p. 80-85.
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	424042-x
ISSN	1872-6283 ; 0379-0738
ISSN (online)	1872-6283
ISSN	0379-0738
DOI	10.1016/j.forsciint.2017.11.025
Database	NAL-Catalogue (AGRICOLA)

In stock of ZB MED Cologne/Königswinter

Zs.B 1104: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: Simple multispectral imaging approach for determining the transfer of explosive residues in consecutive fingerprints

Lees, Heidi / Félix Zapata / Merike Vaher / Carmen García-Ruiz

Talanta. 2018 July 01, v. 184

2018

Abstract: This novel investigation focused on studying the transfer of explosive residues (TNT, HMTD, PETN, ANFO, dynamite, black powder, NH4NO3, KNO3, NaClO3) in ten consecutive fingerprints to two different surfaces – cotton fabric and polycarbonate plastic – by ...

Abstract	This novel investigation focused on studying the transfer of explosive residues (TNT, HMTD, PETN, ANFO, dynamite, black powder, NH4NO3, KNO3, NaClO3) in ten consecutive fingerprints to two different surfaces – cotton fabric and polycarbonate plastic – by using multispectral imaging (MSI). Imaging was performed employing a reflex camera in a purpose-built photo studio. Images were processed in MATLAB to select the most discriminating frame – the one that provided the sharpest contrast between the explosive and the material in the red-green-blue (RGB) visible region. The amount of explosive residues transferred in each fingerprint was determined as the number of pixels containing explosive particles. First, the pattern of PETN transfer by ten different persons in successive fingerprints was studied. No significant differences in the pattern of transfer of PETN between subjects were observed, which was also confirmed by multivariate analysis of variance (MANOVA). Then, the transfer of traces of the nine above explosives in ten consecutive fingerprints to cotton fabric and polycarbonate plastic was investigated. The obtained results demonstrated that the amount of explosive residues deposited on successive fingerprints tended to undergo a power or exponential decrease, with the exception of inorganic salts (NH4NO3, KNO3, NaClO3) and ANFO (consists of 90% NH4NO3).
Keywords	ammonium nitrate ; cameras ; cotton fabric ; explosives ; image analysis ; multispectral imagery ; multivariate analysis ; plastics ; potassium nitrate
Language	English
Dates of publication	2018-0701
Size	p. 437-445.
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	1500969-5
ISSN	1873-3573 ; 0039-9140
ISSN (online)	1873-3573
ISSN	0039-9140
DOI	10.1016/j.talanta.2018.02.079
Database	NAL-Catalogue (AGRICOLA)

Order via subito

Article: A microdestructive capillary electrophoresis method for the analysis of blue-pen-ink strokes on office paper

Calcerrada, Matías / Carmen Garcia-Ruiz / Miguel González-Herráez

Journal of chromatography. 2015 June 26, v. 1400

2015

Abstract: This manuscript describes the development of a capillary electrophoresis (CE) method for the detection of acid and basic dyes and its application to real samples, blue-pen-ink strokes on office paper. First, a capillary zone electrophoresis (CZE) method ... ...

Abstract	This manuscript describes the development of a capillary electrophoresis (CE) method for the detection of acid and basic dyes and its application to real samples, blue-pen-ink strokes on office paper. First, a capillary zone electrophoresis (CZE) method was developed for the separation of basic and acid dyes, by studying the separation medium (buffer nature, pH and relative amount of additive) and instrumental parameters (temperature, voltage and capillary dimensions). The method performance was evaluated in terms of selectivity, resolution (above 5 and 2 for acid dyes and basic dyes, respectively, except for two basic dye standards), LOD (lower than 0.4mg/L) and precision as intraday and interday RSD values of peak migration times (lower than 0.6%). The developed method was then applied to 34 blue pens from different technologies (rollerball, ballpoint, markers) and with different ink composition (gel, water-based, oil-based). A microdestructive sample treatment using a scalpel to scratch 0.3mg of ink stroke was performed. The entire electropherogram profile allowed the visual discrimination between different types of ink and brands, being not necessary a statistical treatment. A 100% of discrimination was achieved between pen technologies, brands, and models, although non-reproducible zones in the electropherograms were found for blue gel pen samples. The two different batches of blue oil-based pens were also differentiated. Thus, this method provides a simple, microdestructive, and rapid analysis of different blue pen technologies which may complement the current analysis of questioned documents performed by forensic laboratories.
Keywords	capillary zone electrophoresis ; chromatography ; dyes ; electric power ; forensic sciences ; gels ; models ; paper ; pH ; rapid methods ; temperature
Language	English
Dates of publication	2015-0626
Size	p. 140-148.
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	218139-3
ISSN	0021-9673 ; 0378-4355 ; 0376-737X
ISSN	0021-9673 ; 0378-4355 ; 0376-737X
DOI	10.1016/j.chroma.2015.04.036
Database	NAL-Catalogue (AGRICOLA)

In stock of ZB MED Cologne/Königswinter

Zs.A 813: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

To top

Full text online

More links

Kategorien

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito