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Book ; Online: Diagnosis, Epidemiology and Transmission Dynamics of Cryptosporidium spp. and Giardia duodenalis

Carmena, David / González-Barrio, David / Köster, Pamela Carolina

2022

Keywords	Research & information: general ; Biology, life sciences ; Cryptosporidium ; genotype ; Bactrian camels ; zoonotic potential ; public health ; Cryptosporidium parvum ; subtype ; bamboo rat ; human pathogen ; Cryptosporidium felis ; 60-kDa glycoprotein ; subtypes ; zoonotic transmission ; Giardia duodenalis ; coypus (Myocastor coypus) ; multi-locus genotype ; genetic variation ; zoonotic genotypes ; PCR (polymerase chain reaction) ; China ; Giardia ; Brazil ; Amazon ; asymptomatic ; community ; genotyping ; indigenous ; risk association ; Tapirapé ; transmission ; Blastocystis ; enteric parasites ; children ; diarrhoea ; PCR ; molecular epidemiology ; Mozambique ; acute diarrhea ; risk factor ; gp60 ; ssu rRNA ; prevalence ; GEMS ; parasite ; parasitology ; epidemiology ; genetic diversity ; host specificity ; Europe ; Scandinavia ; protist ; sporozoa ; zoonosis ; detection ; diagnosis ; sensitivity ; specificity ; coinfection ; enteric protists ; Entamoeba histolytica ; Blastocystis sp ; molecular diversity ; Cryptosporidium cuniculus ; rabbits ; Egypt ; gp60 gene ; PCR-RFLP ; zoonoses ; Cryptosporidium xiaoi ; subtyping ; host adaptation ; Enterocytozoon bieneusi ; Cryptosporidium spp ; pet dogs and cats ; Yunnan province ; n/a
Size	1 electronic resource (216 pages)
Publisher	MDPI - Multidisciplinary Digital Publishing Institute
Publishing place	Basel
Document type	Book ; Online
Note	English ; Open Access
HBZ-ID	HT021291356
ISBN	9783036530789 ; 3036530789
Database	ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

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Article: Editorial for the Special Issue: Diagnosis, Epidemiology and Transmission Dynamics of

Köster, Pamela C / González-Barrio, David / Carmena, David

Pathogens (Basel, Switzerland)

2022 Volume 11, Issue 2

Abstract: ... ...

Abstract	Cryptosporidium
Language	English
Publishing date	2022-01-24
Publishing country	Switzerland
Document type	Editorial
ZDB-ID	2695572-6
ISSN	2076-0817
ISSN	2076-0817
DOI	10.3390/pathogens11020141
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Giardia duodenalis: Detection by Quantitative Real-Time PCR and Molecular Diversity.

Dashti, Alejandro / Köster, Pamela C / Carmena, David

Methods in molecular biology (Clifton, N.J.)

2021 Volume 2369, Page(s) 83–97

Abstract: Conventional microscopy is being progressively replaced by molecular (PCR and Sanger sequencing) methods for the first-line detection, identification, and genotyping of diarrhea-causing enteric parasites in modern clinical and research laboratories. ... ...

Abstract	Conventional microscopy is being progressively replaced by molecular (PCR and Sanger sequencing) methods for the first-line detection, identification, and genotyping of diarrhea-causing enteric parasites in modern clinical and research laboratories. These technologies allow increased sample testing, improved throughput, and optimized laboratory workflow in a cost-effective manner. Here, we describe the methodological algorithm used in a national reference center for the diagnosis and molecular characterization of Giardia duodenalis, a major contributor to the enormous burden of diarrheal disease globally. Initial detection of the parasite is conducted by a sensitive real-time PCR to amplify the small subunit of the ribosomal RNA of G. duodenalis, whereas a multilocus sequence genotyping scheme based on three constitutive genetic markers (glutamate dehydrogenase, β-giardin, and triose phosphate isomerase) is used for genotyping and sub-genotyping purposes. The advantages and disadvantages of these PCR-based methods are commented.
MeSH term(s)	Diarrhea ; Feces ; Genotype ; Giardia lamblia/genetics ; Giardiasis/diagnosis ; Glutamate Dehydrogenase/genetics ; Humans ; Multilocus Sequence Typing ; RNA, Ribosomal ; Real-Time Polymerase Chain Reaction
Chemical Substances	RNA, Ribosomal ; Glutamate Dehydrogenase (EC 1.4.1.2)
Language	English
Publishing date	2021-07-27
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1940-6029
ISSN (online)	1940-6029
DOI	10.1007/978-1-0716-1681-9_6
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Evaluation of the Use of Singleplex and Duplex CerTest VIASURE Real-Time PCR Assays to Detect Common Intestinal Protist Parasites.

Dashti, Alejandro / Alonso, Henar / Escolar-Miñana, Cristina / Köster, Pamela C / Bailo, Begoña / Carmena, David / González-Barrio, David

Diagnostics (Basel, Switzerland)

2024 Volume 14, Issue 3

Abstract: ... ...

Abstract	Cryptosporidium
Language	English
Publishing date	2024-02-01
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2662336-5
ISSN	2075-4418
ISSN	2075-4418
DOI	10.3390/diagnostics14030319
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Article: Human-Borne Pathogens: Are They Threatening Wild Great Ape Populations?

Köster, Pamela C. / Lapuente, Juan / Cruz, Israel / Carmena, David / Ponce-Gordo, Francisco

Veterinary sciences. 2022 July 13, v. 9, no. 7

2022

Abstract: Climate change and anthropic activities are the two main factors explaining wild great ape habitat reduction and population decline. The extent to which human-borne infectious diseases are contributing to this trend is still poorly understood. This is ... ...

Abstract	Climate change and anthropic activities are the two main factors explaining wild great ape habitat reduction and population decline. The extent to which human-borne infectious diseases are contributing to this trend is still poorly understood. This is due to insufficient or fragmented knowledge on the abundance and distribution of current wild great ape populations, the difficulty obtaining optimal biological samples for diagnostic testing, and the scarcity of pathogen typing data of sufficient quality. This review summarises current information on the most clinically relevant pathogens of viral, bacterial, parasitic, and fungal nature for which transmission from humans to wild great apes is suspected. After appraising the robustness of available epidemiological and/or molecular typing evidence, we attempt to categorise each pathogen according to its likelihood of truly being of human origin. We further discuss those agents for which anthroponotic transmission is more likely. These include two viral (Human Metapneumovirus and Respiratory Syncytial Virus), one bacterial (diarrhoeagenic Escherichia coli), and two parasitic (Cryptosporidium spp. and Giardia duodenalis) pathogens. Finally, we identify the main drawbacks impairing research on anthroponotic pathogen transmission in wild great apes and propose research lines that may contribute to bridging current knowledge gaps.
Keywords	Cryptosporidium ; Giardia lamblia ; Human metapneumovirus ; Pongidae ; Respiratory syncytial virus ; climate change ; disease transmission ; enterovirulent Escherichia coli group ; fungi ; habitats ; humans ; pathogens ; population dynamics
Language	English
Dates of publication	2022-0713
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article
ZDB-ID	2768971-2
ISSN	2306-7381
ISSN	2306-7381
DOI	10.3390/vetsci9070356
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Cryptosporidium hominis infections in non-human animal species: revisiting the concept of host specificity.

Widmer, Giovanni / Köster, Pamela C / Carmena, David

International journal for parasitology

2020 Volume 50, Issue 4, Page(s) 253–262

Abstract: Parasites in the genus Cryptosporidium, phylum Apicomplexa, are found worldwide in the intestinal tract of many vertebrate species and in the environment. Driven by sensitive PCR methods, and the availability of abundant sequence data and reference ... ...

Abstract	Parasites in the genus Cryptosporidium, phylum Apicomplexa, are found worldwide in the intestinal tract of many vertebrate species and in the environment. Driven by sensitive PCR methods, and the availability of abundant sequence data and reference genomes, the taxonomic complexity of the genus has steadily increased; 38 species have been named to date. Due to its public health importance, Cryptosporidium hominis has long attracted the interest of the research community. This species was initially described as infectious to humans only. This perception has persisted in spite of an increasing number of observations of natural and experimental infections of animals with this species. Here we summarize and discuss this literature published since 2000 and conclude that the host range of C. hominis is broader than originally described. The evolving definition of the C. hominis host range raises interesting questions about host specificity and the evolution of Cryptosporidium parasites.
MeSH term(s)	Animals ; Animals, Wild/parasitology ; Cryptosporidiosis/parasitology ; Cryptosporidiosis/transmission ; Cryptosporidium/classification ; Cryptosporidium/genetics ; Cryptosporidium/isolation & purification ; Cryptosporidium parvum/classification ; Cryptosporidium parvum/genetics ; Cryptosporidium parvum/isolation & purification ; DNA, Protozoan/analysis ; Disease Reservoirs ; Genes, Protozoan ; Host Specificity/genetics ; Humans ; Livestock/parasitology ; Phylogeny ; Zoonoses
Chemical Substances	DNA, Protozoan
Language	English
Publishing date	2020-03-20
Publishing country	England
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Review
ZDB-ID	120518-3
ISSN	1879-0135 ; 0020-7519
ISSN (online)	1879-0135
ISSN	0020-7519
DOI	10.1016/j.ijpara.2020.01.005
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Diagnóstico molecular de parasitosis intestinales.

Dacal, Elena / Köster, Pamela C / Carmena, David

Enfermedades infecciosas y microbiologia clinica (English ed.)

2020 Volume 38 Suppl 1, Page(s) 24–31

Abstract: Infections causes by parasites of the gastrointestinal tract are a global public health problem. In industrialised countries, their particular epidemiological (low general prevalence of enteroparasites), economic (high labour costs) and clinical ... ...

Abstract	Infections causes by parasites of the gastrointestinal tract are a global public health problem. In industrialised countries, their particular epidemiological (low general prevalence of enteroparasites), economic (high labour costs) and clinical characteristics (constant increase in the number of samples and diagnostic determinations to be performed) have led molecular techniques to progressively replace conventional microscopy as the first-line diagnostic method of these pathogens in modern clinical laboratories. PCR-based techniques, particularly those developed for the simultaneous detection of the various agents that can cause the same infectious disease (syndromic diagnosis), already represent a cost-effective option that allow process automisation, workflow optimisation, and comparison of results among different laboratories, and facilitate accreditation of diagnostic procedures. This review clearly and concisely discusses the current situation of the molecular diagnosis of the main species of intestinal parasites in humans, particularly the enteric protozoans causing diarrhoea (Cryptosporidium spp., Giardia duodenalis, Entamoeba histolytica), the most important members the Microsporidia phyla (Enterocytozoon bieneusi) and Stramenopiles phyla (Blastocystis sp.), as well as the helminths transmitted by soil (Ancylostoma spp., Ascaris lumbricoides, Necator americanus, Strongyloides stercoralis and Trichuris trichiura) and food (Anisakis spp., Clonorchis sinensis, Fasciola spp., Taenia solium, and Trichinella spiralis). Special attention is paid to the description of available techniques and formats, to their diagnostic benefits and the most widely used genetic markers for their detection, both in clinical laboratories and genotyping in referral and research centres.
MeSH term(s)	Animals ; Diarrhea/parasitology ; Feces ; Helminthiasis/diagnosis ; Humans ; Intestinal Diseases, Parasitic/diagnosis ; Molecular Diagnostic Techniques ; Protozoan Infections/diagnosis ; Protozoan Infections/parasitology ; Real-Time Polymerase Chain Reaction
Language	Spanish
Publishing date	2020-02-28
Document type	Journal Article ; Review
ISSN	2529-993X
ISSN (online)	2529-993X
DOI	10.1016/j.eimc.2020.02.005
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Cryptosporidium hominis infections in non-human animal species: revisiting the concept of host specificity

Widmer, Giovanni / Köster, Pamela C / Carmena, David

Australian Society for Parasitology International journal for parasitology. 2020 Apr., v. 50, no. 4

2020

Abstract	Parasites in the genus Cryptosporidium, phylum Apicomplexa, are found worldwide in the intestinal tract of many vertebrate species and in the environment. Driven by sensitive PCR methods, and the availability of abundant sequence data and reference genomes, the taxonomic complexity of the genus has steadily increased; 38 species have been named to date. Due to its public health importance, Cryptosporidium hominis has long attracted the interest of the research community. This species was initially described as infectious to humans only. This perception has persisted in spite of an increasing number of observations of natural and experimental infections of animals with this species. Here we summarize and discuss this literature published since 2000 and conclude that the host range of C. hominis is broader than originally described. The evolving definition of the C. hominis host range raises interesting questions about host specificity and the evolution of Cryptosporidium parasites.
Keywords	Cryptosporidium hominis ; animal diseases ; evolution ; genome ; host range ; host specificity ; intestines ; parasites ; polymerase chain reaction ; public health
Language	English
Dates of publication	2020-04
Size	p. 253-262.
Publishing place	Elsevier Ltd
Document type	Article
ZDB-ID	120518-3
ISSN	1879-0135 ; 0020-7519
ISSN (online)	1879-0135
ISSN	0020-7519
DOI	10.1016/j.ijpara.2020.01.005
Database	NAL-Catalogue (AGRICOLA)

In stock of ZB MED Cologne/Königswinter

Zs.A 789: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

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Article ; Online: Human-Borne Pathogens: Are They Threatening Wild Great Ape Populations?

Köster, Pamela C / Lapuente, Juan / Cruz, Israel / Carmena, David / Ponce-Gordo, Francisco

Veterinary sciences

2022 Volume 9, Issue 7

Abstract	Climate change and anthropic activities are the two main factors explaining wild great ape habitat reduction and population decline. The extent to which human-borne infectious diseases are contributing to this trend is still poorly understood. This is due to insufficient or fragmented knowledge on the abundance and distribution of current wild great ape populations, the difficulty obtaining optimal biological samples for diagnostic testing, and the scarcity of pathogen typing data of sufficient quality. This review summarises current information on the most clinically relevant pathogens of viral, bacterial, parasitic, and fungal nature for which transmission from humans to wild great apes is suspected. After appraising the robustness of available epidemiological and/or molecular typing evidence, we attempt to categorise each pathogen according to its likelihood of truly being of human origin. We further discuss those agents for which anthroponotic transmission is more likely. These include two viral (Human Metapneumovirus and Respiratory Syncytial Virus), one bacterial (diarrhoeagenic
Language	English
Publishing date	2022-07-13
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2768971-2
ISSN	2306-7381 ; 2306-7381
ISSN (online)	2306-7381
ISSN	2306-7381
DOI	10.3390/vetsci9070356
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Prevalence and associated risk factors of intestinal parasites among schoolchildren in Ecuador, with emphasis on the molecular diversity of Giardia duodenalis, Blastocystis sp. and Enterocytozoon bieneusi.

Tapia-Veloz, Estephany / Gozalbo, Mónica / Guillén, Marisa / Dashti, Alejandro / Bailo, Begoña / Köster, Pamela C / Santín, Mónica / Carmena, David / Trelis, María

PLoS neglected tropical diseases

2023 Volume 17, Issue 5, Page(s) e0011339

Abstract: Background: Intestinal helminths, including Soil-Transmitted Helminth (STH), and Gastrointestinal Protist (GP) infections are major contributors to the global burden of disease, particularly in low-income countries such Ecuador. Their epidemiology in ... ...

Abstract	Background: Intestinal helminths, including Soil-Transmitted Helminth (STH), and Gastrointestinal Protist (GP) infections are major contributors to the global burden of disease, particularly in low-income countries such Ecuador. Their epidemiology in these settings is largely unknown. Methodology: This prospective cross-sectional study investigates the carriage of intestinal helminths, including STH, and GP in asymptomatic schoolchildren (3-11 years) in the Chimborazo and Guayas provinces, Ecuador. Single stool samples (n = 372) and epidemiological questionnaires on demographics and potential risk factors were collected from participating schoolchildren. Conventional microscopy examination was used as screening method, and molecular (PCR and Sanger sequencing) assays were used to further investigate the epidemiology of some GP. A multivariate logistic regression analysis was used to evaluate the strength of the association of suspected risk factors with the presence of helminths and GP. Principal findings: At least one intestinal parasite species was observed by microscopy in 63.2% (235/372) of the participating schoolchildren. Enterobius vermicularis (16.7%, 62/372; 95% CI: 13.0-20.9) and Blastocystis sp. (39.2%, 146/372; 95% CI: 34.2-44.2) were the most prevalent among helminths and GP, respectively. Assemblages A (50.0%), B (37.5%) and A+B (12.5%) were detected within Giardia duodenalis and ST3 (28.6%), ST1 and ST2 (26.2% each), and ST4 (14.3%) within Blastocystis sp. Three genotypes, two known (A: 66.7%; KB-1: 16.7%) and a novel (HhEcEb1, 16.7%) were identified within Enterocytozoon bieneusi. Municipality of origin, household overcrowding, and poor sanitation and personal hygiene habits were risk factors for childhood intestinal parasites colonization. Conclusions/significance: Despite massive government drug administration programs, STH and GP infection remain a public health concern in paediatric populations living in poor-resource settings. Molecular analytical methods are required to better understand the epidemiology of these intestinal parasites. This study provides novel information on the occurrence of Blastocystis sp. and E. bieneusi genetic variants circulating in Ecuadorian human populations.
MeSH term(s)	Child ; Animals ; Humans ; Giardia lamblia/genetics ; Blastocystis/genetics ; Parasites ; Enterocytozoon ; Ecuador/epidemiology ; Prevalence ; Cross-Sectional Studies ; Prospective Studies ; Blastocystis Infections/epidemiology ; Blastocystis Infections/parasitology ; Helminths ; Risk Factors ; Feces/parasitology
Language	English
Publishing date	2023-05-24
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2429704-5
ISSN	1935-2735 ; 1935-2735
ISSN (online)	1935-2735
ISSN	1935-2735
DOI	10.1371/journal.pntd.0011339
Database	MEDical Literature Analysis and Retrieval System OnLINE

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