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Article ; Online: Corynebacterium ramonii sp. nov., a novel toxigenic member of the Corynebacterium diphtheriae species complex.

Crestani, Chiara / Arcari, Gabriele / Landier, Annie / Passet, Virginie / Garnier, Dorian / Brémont, Sylvie / Armatys, Nathalie / Carmi-Leroy, Annick / Toubiana, Julie / Badell, Edgar / Brisse, Sylvain

Research in microbiology

2023 Volume 174, Issue 7, Page(s) 104113

Abstract: The Corynebacterium diphtheriae species complex comprises seven bacterial species, including Corynebacterium ulcerans, a zoonotic pathogen from multiple animal species. In this work, we characterise phenotypically and genotypically isolates belonging to ... ...

Abstract	The Corynebacterium diphtheriae species complex comprises seven bacterial species, including Corynebacterium ulcerans, a zoonotic pathogen from multiple animal species. In this work, we characterise phenotypically and genotypically isolates belonging to two C. ulcerans lineages. Results from phylogenetic analyses, in silico DNA-DNA hybridization (DDH) and MALDI-TOF spectra differentiate lineage 2 from C. ulcerans lineage 1, which, together with their distinct transmission dynamics (probable human-to-human vs animal-to-human), indicates that lineage 2 is a separate Corynebacterium species, which we propose to name Corynebacterium ramonii. This species is of particular medical interest considering that its human-to-human transmission is likely, and that some C. ramonii isolates carry the diphtheria toxin gene.
Language	English
Publishing date	2023-08-10
Publishing country	France
Document type	Journal Article
ZDB-ID	1004220-9
ISSN	1769-7123 ; 0923-2508
ISSN (online)	1769-7123
ISSN	0923-2508
DOI	10.1016/j.resmic.2023.104113
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Article ; Online: Taxonomic status of Corynebacterium diphtheriae biovar Belfanti and proposal of Corynebacterium belfantii sp. nov.

Dazas, Melody / Badell, Edgar / Carmi-Leroy, Annick / Criscuolo, Alexis / Brisse, Sylvain

International journal of systematic and evolutionary microbiology

2018 Volume 68, Issue 12, Page(s) 3826–3831

Abstract: Clinical isolates belonging to Corynebacterium diphtheriae biovar Belfanti were characterized by genomic sequencing and biochemical and chemotaxonomic analyses. Phylogenetic analyses indicated that biovar Belfanti represents a branch that is clearly ... ...

Abstract	Clinical isolates belonging to Corynebacterium diphtheriae biovar Belfanti were characterized by genomic sequencing and biochemical and chemotaxonomic analyses. Phylogenetic analyses indicated that biovar Belfanti represents a branch that is clearly demarcated from C. diphtheriae strains of biovars Mitis and Gravis. The average nucleotide identity of isolates of biovar Belfanti with C. diphtheriae type strain NCTC 11397
MeSH term(s)	Bacterial Typing Techniques ; Base Composition ; Corynebacterium diphtheriae/classification ; Corynebacterium diphtheriae/genetics ; Corynebacterium diphtheriae/isolation & purification ; DNA, Bacterial/genetics ; Fatty Acids/chemistry ; France ; Genes, Bacterial ; Humans ; Phospholipids/chemistry ; Phylogeny ; RNA, Ribosomal, 16S/genetics ; Respiratory System/microbiology ; Sequence Analysis, DNA ; Vitamin K 2/chemistry
Chemical Substances	DNA, Bacterial ; Fatty Acids ; Phospholipids ; RNA, Ribosomal, 16S ; Vitamin K 2 (11032-49-8)
Language	English
Publishing date	2018-10-24
Publishing country	England
Document type	Journal Article
ZDB-ID	2002336-4
ISSN	1466-5034 ; 1466-5026
ISSN (online)	1466-5034
ISSN	1466-5026
DOI	10.1099/ijsem.0.003069
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Article ; Online: A global Corynebacterium diphtheriae genomic framework sheds light on current diphtheria reemergence

Hennart, Mélanie / Crestani, Chiara / Bridel, Sébastien / Armatys, Nathalie / Brémont, Sylvie / Carmi-Leroy, Annick / Landier, Annie / Passet, Virginie / Fonteneau, Laure / Vaux, Sophie / Toubiana, Julie / Badell, Edgar / Brisse, Sylvain

Peer Community Journal, Vol 3, Iss , Pp - (2023)

2023

Abstract: Background: Diphtheria, caused by Corynebacterium diphtheriae, reemerges in Europe since 2022. Genomic sequencing can inform on transmission routes and genotypes of concern, but currently, no standard approach exists to detect clinically important ... ...

Abstract	Background: Diphtheria, caused by Corynebacterium diphtheriae, reemerges in Europe since 2022. Genomic sequencing can inform on transmission routes and genotypes of concern, but currently, no standard approach exists to detect clinically important genomic features and to interpret emergence in the global C. diphtheriae population framework. Methods: We developed the bioinformatics pipeline diphtOscan (available at https://gitlab.pasteur.fr/BEBP/diphtoscan) to extract from genomes of Corynebacteria of the diphtheriae species complex, medically relevant features including tox gene presence and disruption. We analyzed 101 human C. diphtheriae isolates collected in 2022 in metropolitan and overseas France (France-2022). To define the population background of this emergence, we sequenced 379 additional isolates (mainly from France, 2018-2021) and collated 870 publicly-available genomes. Results: The France-2022 isolates comprised 45 tox-positive (44 toxigenic) isolates, mostly imported, belonging to 10 sublineages (<500 distinct core genes). The global dataset comprised 245 sublineages and 33.9% tox-positive genomes, with diphtOscan predicting non-toxigenicity in 16.0% of these. 12% of the global isolates, and 43.6% of France-2022 ones, were multidrug resistant. Convergence of toxigenicity with penicillin and erythromycin resistance was observed in 2 isolates from France-2022. Phylogenetic lineages Gravis and Mitis contrasted strikingly in their pathogenicity-associated genes. Conclusions: This work provides a bioinformatics tool and global population framework to analyze C. diphtheriae genomes, revealing important heterogeneities in virulence and resistance features. Emerging genotypes combining toxigenicity and first-line antimicrobial resistance represent novel threats. Genomic epidemiology studies of C. diphtheriae should be intensified globally to improve understanding of reemergence and spatial spread.
Keywords	diphtheria ; genomic sequencing ; antimicrobial resistance ; virulence ; epidemiology ; transmission ; 2022 reemergence ; bioinformatics tool ; Archaeology ; CC1-960 ; Science ; Q
Language	English
Publishing date	2023-08-01T00:00:00Z
Publisher	Peer Community In
Document type	Article ; Online
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Article: Corynebacterium rouxii sp. nov., a novel member of the diphtheriae species complex

Badell, Edgar / Hennart, Mélanie / Rodrigues, Carla / Passet, Virginie / Dazas, Melody / Panunzi, Leonardo / Bouchez, Valérie / Carmi-Leroy, Annick / Toubiana, Julie / Brisse, Sylvain

Research in microbiology. 2020 Feb. 19,

2020

Abstract: A group of six clinical isolates previously identified as Corynebacterium diphtheriae biovar Belfanti, isolated from human cutaneous or peritoneum infections and from one dog, were characterized by genomic sequencing, biochemical analysis and MALDI-TOF ... ...

Abstract	A group of six clinical isolates previously identified as Corynebacterium diphtheriae biovar Belfanti, isolated from human cutaneous or peritoneum infections and from one dog, were characterized by genomic sequencing, biochemical analysis and MALDI-TOF mass spectrometry. The six isolates were negative for the diphtheria toxin gene. Phylogenetic analyses showed that the six isolates (including FRC0190T) are clearly demarcated from C. diphtheriae, C. belfantii, C. ulcerans and C. pseudotuberculosis. The average nucleotide identity of FRC0190T with C. diphtheriae NCTC11397T was 92.6%, and was 91.8% with C. belfantii FRC0043T. C. diphtheriae subsp. lausannense strain CHUV2995T appeared to be a later heterotypic synonym of C. belfantii (ANI, 99.3%). Phenotyping data revealed an atypical negative or heterogeneous intermediate maltose fermentation reaction for the six isolates. MALDI-TOF mass spectrometry differentiated the new group from the other Corynebacterium taxa by the presence of specific spectral peaks. rpoB sequences showed identity to atypical, maltose-negative C. diphtheriae biovar Belfanti isolates previously described from two cats in the USA. We propose the name Corynebacterium rouxii sp. nov. for the novel group, with FRC0190T (= CIP 111752T = DSM 110354T) as type strain.
Keywords	Corynebacterium diphtheriae ; Corynebacterium pseudotuberculosis ; Corynebacterium ulcerans ; cats ; diphtheria toxin ; dogs ; fermentation ; genes ; humans ; maltose ; matrix-assisted laser desorption-ionization mass spectrometry ; new species ; peritoneum ; phenotype ; phylogeny ; sequence analysis ; United States
Language	English
Dates of publication	2020-0219
Publishing place	Elsevier Masson SAS
Document type	Article
Note	Pre-press version
ZDB-ID	1004220-9
ISSN	1769-7123 ; 0923-2508
ISSN (online)	1769-7123
ISSN	0923-2508
DOI	10.1016/j.resmic.2020.02.003
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Article ; Online: Population genomics and antimicrobial resistance in Corynebacterium diphtheriae.

Hennart, Melanie / Panunzi, Leonardo G / Rodrigues, Carla / Gaday, Quentin / Baines, Sarah L / Barros-Pinkelnig, Marina / Carmi-Leroy, Annick / Dazas, Melody / Wehenkel, Anne Marie / Didelot, Xavier / Toubiana, Julie / Badell, Edgar / Brisse, Sylvain

Genome medicine

2020 Volume 12, Issue 1, Page(s) 107

Abstract: Background: Corynebacterium diphtheriae, the agent of diphtheria, is a genetically diverse bacterial species. Although antimicrobial resistance has emerged against several drugs including first-line penicillin, the genomic determinants and population ... ...

Abstract	Background: Corynebacterium diphtheriae, the agent of diphtheria, is a genetically diverse bacterial species. Although antimicrobial resistance has emerged against several drugs including first-line penicillin, the genomic determinants and population dynamics of resistance are largely unknown for this neglected human pathogen. Methods: Here, we analyzed the associations of antimicrobial susceptibility phenotypes, diphtheria toxin production, and genomic features in C. diphtheriae. We used 247 strains collected over several decades in multiple world regions, including the 163 clinical isolates collected prospectively from 2008 to 2017 in France mainland and overseas territories. Results: Phylogenetic analysis revealed multiple deep-branching sublineages, grouped into a Mitis lineage strongly associated with diphtheria toxin production and a largely toxin gene-negative Gravis lineage with few toxin-producing isolates including the 1990s ex-Soviet Union outbreak strain. The distribution of susceptibility phenotypes allowed proposing ecological cutoffs for most of the 19 agents tested, thereby defining acquired antimicrobial resistance. Penicillin resistance was found in 17.2% of prospective isolates. Seventeen (10.4%) prospective isolates were multidrug-resistant (≥ 3 antimicrobial categories), including four isolates resistant to penicillin and macrolides. Homologous recombination was frequent (r/m = 5), and horizontal gene transfer contributed to the emergence of antimicrobial resistance in multiple sublineages. Genome-wide association mapping uncovered genetic factors of resistance, including an accessory penicillin-binding protein (PBP2m) located in diverse genomic contexts. Gene pbp2m is widespread in other Corynebacterium species, and its expression in C. glutamicum demonstrated its effect against several beta-lactams. A novel 73-kb C. diphtheriae multiresistance plasmid was discovered. Conclusions: This work uncovers the dynamics of antimicrobial resistance in C. diphtheriae in the context of phylogenetic structure, biovar, and diphtheria toxin production and provides a blueprint to analyze re-emerging diphtheria.
MeSH term(s)	Anti-Bacterial Agents/pharmacology ; Corynebacterium diphtheriae/classification ; Corynebacterium diphtheriae/drug effects ; Corynebacterium diphtheriae/genetics ; Corynebacterium diphtheriae/isolation & purification ; DNA, Bacterial/genetics ; Diphtheria/microbiology ; Diphtheria Toxin/genetics ; Drug Resistance, Bacterial/genetics ; Genes, Bacterial/genetics ; Genome-Wide Association Study ; Genomics ; Humans ; Macrolides/pharmacology ; Metagenomics ; Microbial Sensitivity Tests ; Multilocus Sequence Typing ; Phylogeny ; Prospective Studies
Chemical Substances	Anti-Bacterial Agents ; DNA, Bacterial ; Diphtheria Toxin ; Macrolides
Language	English
Publishing date	2020-11-27
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2484394-5
ISSN	1756-994X ; 1756-994X
ISSN (online)	1756-994X
ISSN	1756-994X
DOI	10.1186/s13073-020-00805-7
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Article ; Online: Corynebacterium rouxii sp. nov., a novel member of the diphtheriae species complex.

Badell, Edgar / Hennart, Mélanie / Rodrigues, Carla / Passet, Virginie / Dazas, Melody / Panunzi, Leonardo / Bouchez, Valérie / Carmi-Leroy, Annick / Toubiana, Julie / Brisse, Sylvain

Research in microbiology

2020 Volume 171, Issue 3-4, Page(s) 122–127

Abstract	A group of six clinical isolates previously identified as Corynebacterium diphtheriae biovar Belfanti, isolated from human cutaneous or peritoneum infections and from one dog, were characterized by genomic sequencing, biochemical analysis and MALDI-TOF mass spectrometry. The six isolates were negative for the diphtheria toxin gene. Phylogenetic analyses showed that the six isolates (including FRC0190
MeSH term(s)	Bacterial Typing Techniques ; Corynebacterium/chemistry ; Corynebacterium/classification ; Corynebacterium/genetics ; Corynebacterium Infections/microbiology ; Corynebacterium diphtheriae/chemistry ; Corynebacterium diphtheriae/classification ; Corynebacterium diphtheriae/genetics ; Humans ; Phylogeny ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; Whole Genome Sequencing
Language	English
Publishing date	2020-02-28
Publishing country	France
Document type	Journal Article
ZDB-ID	1004220-9
ISSN	1769-7123 ; 0923-2508
ISSN (online)	1769-7123
ISSN	0923-2508
DOI	10.1016/j.resmic.2020.02.003
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Article ; Online: Ongoing diphtheria outbreak in Yemen: a cross-sectional and genomic epidemiology study.

Badell, Edgar / Alharazi, Abdulilah / Criscuolo, Alexis / Almoayed, Khaled Abdullah Ali / Lefrancq, Noémie / Bouchez, Valerie / Guglielmini, Julien / Hennart, Melanie / Carmi-Leroy, Annick / Zidane, Nora / Pascal-Perrigault, Marine / Lebreton, Manon / Martini, Helena / Salje, Henrik / Toubiana, Julie / Dureab, Fekri / Dhabaan, Ghulam / Brisse, Sylvain

The Lancet. Microbe

2021 Volume 2, Issue 8, Page(s) e386–e396

Abstract: Background: An outbreak of diphtheria, declared in Yemen in October, 2017, is ongoing. We did a cross-sectional study to investigate the epidemiological, clinical, and microbiological features of the outbreak.: Methods: Probable cases of diphtheria ... ...

Abstract	Background: An outbreak of diphtheria, declared in Yemen in October, 2017, is ongoing. We did a cross-sectional study to investigate the epidemiological, clinical, and microbiological features of the outbreak. Methods: Probable cases of diphtheria that were defined clinically and recorded through a weekly electronic diseases early warning system (from 2017, week 22, to 2020, week 17) were used to identify trends of the outbreak (we divided the epidemic into three time periods: May 29, 2017, to June 10, 2018; June 11, 2018, to June 3, 2019; and June 4, 2019, to April 26, 2020). We used the line list of diphtheria reports for governorate-level descriptions. Vaccination coverage was estimated using the 2017 and 2018 annual reports by the national Expanded Programme on Immunization. To confirm cases biologically, Corynebacterium diphtheriae was isolated and identified from throat swabs using standard microbiological culture and identification procedures. We assessed differences in the temporal and geographical distributions of cases, including between different age groups. For in-depth microbiological analysis, tox gene and species-specific rpoB real-time PCR, Illumina genomic sequencing, antimicrobial susceptibility analysis (disk diffusion, E-test), and the Elek diphtheria toxin production test were done on confirmed cases. We used genomic data for phylogenetic analyses and to estimate the nucleotide substitution rate. Findings: The Yemen diphtheria outbreak affected almost all governorates (provinces), with 5701 probable cases and 330 deaths recorded up to April 26, 2020. We collected clinical data for 888 probable cases with throat swab samples referred for biological confirmation, and genomic data for 42 positive cases, corresponding to 43 isolates (two isolates from one culture were included due to distinct colony morphologies). The median age of patients was 12 years (range 0·2-80). The proportion of cases in children aged 0-4 years was reduced during the second time period, after a vaccination campaign, compared with the first period (19% [95% CI 18-21] in the first period vs 14% [12-15] in the second period, p<0·0001). Among 43 tested isolates, 39 (91%) produced the diphtheria toxin and two had low level (0·25 mg/L) antimicrobial resistance to penicillin. We identified six C diphtheriae phylogenetic sublineages, four of which are genetically related to isolates from Saudi Arabia, Eritrea, and Somalia. Inter-sublineage genomic variations in genes associated with antimicrobial resistance, iron acquisition, and adhesion were observed. The predominant sublineage (30 [70%] of 43 isolates) was resistant to trimethoprim and was associated with unique genomic features, more frequent neck swelling (p=0·0029) and a younger age of patients (p=0·060) compared with the other sublineages. Its evolutionary rate was estimated at 1·67 × 10 Interpretation: In the Yemen outbreak, C diphtheriae shows high phylogenetic, genomic, and phenotypic variation. Laboratory capacity and real-time microbiological monitoring of diphtheria outbreaks need to be scaled up to inform case management and transmission control of diphtheria. Catch-up vaccination might have provided some protection to the targeted population (children aged 0-4 years). Funding: National Centre of the Public Health Laboratories (Yemen), Institut Pasteur, and the French Government Investissement d'Avenir Programme. Translation: For the Arabic translation of the abstract see Supplementary Materials section.
MeSH term(s)	Adolescent ; Adult ; Aged ; Aged, 80 and over ; Anti-Infective Agents ; Child ; Child, Preschool ; Corynebacterium ; Corynebacterium diphtheriae/genetics ; Cross-Sectional Studies ; Diphtheria/epidemiology ; Diphtheria Toxin/genetics ; Disease Outbreaks ; Genomics ; Humans ; Infant ; Middle Aged ; Phylogeny ; Yemen/epidemiology ; Young Adult
Chemical Substances	Anti-Infective Agents ; Diphtheria Toxin
Language	English
Publishing date	2021-05-26
Publishing country	England
Document type	Journal Article
ISSN	2666-5247
ISSN (online)	2666-5247
DOI	10.1016/S2666-5247(21)00094-X
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Article ; Online: New markers in Anopheles gambiae salivary glands after Plasmodium berghei infection.

Zocevic, Aleksandar / Carmi-Leroy, Annick / Sautereau, Jean / d'Alayer, Jacques / Lenormand, P / Rousselle, J-C / Namane, A / Choumet, Valérie

Vector borne and zoonotic diseases (Larchmont, N.Y.)

2013 Volume 13, Issue 2, Page(s) 119–127

Abstract: In malaria, mosquito saliva and salivary glands play central roles in the multi-faceted interactions that occur among the parasite, its vector, and its host. Analyzing the processes involved in the survival and maintenance of the Plasmodium parasite in ... ...

Abstract	In malaria, mosquito saliva and salivary glands play central roles in the multi-faceted interactions that occur among the parasite, its vector, and its host. Analyzing the processes involved in the survival and maintenance of the Plasmodium parasite in mosquito organs, and in its transmission into vertebrate hosts, may lead to the identification of new molecular targets for parasite control. We used comparative two-dimensional gel polyacrylamide electrophoresis (2D-PAGE), surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS), and high-performance liquid chromatography (HPLC), followed by Edman sequencing, to study saliva and salivary gland samples from Anopheles gambiae mosquitoes infected or not with Plasmodium berghei. Quantitative 2D-PAGE profile analysis showed that the intensities of seven spots were affected by the presence of the parasite in the salivary glands. Most of the proteins identified possessed a signal peptide. SELDI-TOF-MS revealed 32 proteins/peptides whose peak intensities differed between the Plasmodium-infected and non-infected control groups. Quantitative comparison of HPLC profiles of low-molecular-weight components from salivary gland extracts revealed several peptides and proteins with levels that were modulated by parasite infection. The results of these complementary approaches suggest that the infection of female A. gambiae mosquitoes by P. berghei alters the production levels of several salivary gland proteins and peptides, some of which (e.g., protein cE5, B3VDI9_ANOGA, and AGAP008216-PA) are known or predicted to be secreted in saliva and involved in blood feeding.
MeSH term(s)	Animals ; Anopheles/metabolism ; Anopheles/microbiology ; Biomarkers ; Chromatography, High Pressure Liquid ; Electrophoresis, Gel, Two-Dimensional ; Female ; Host-Parasite Interactions ; Plasmodium berghei/physiology ; Salivary Glands/metabolism ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Chemical Substances	Biomarkers
Language	English
Publishing date	2013-02
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2047199-3
ISSN	1557-7759 ; 1530-3667
ISSN (online)	1557-7759
ISSN	1530-3667
DOI	10.1089/vbz.2012.0964
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Article ; Online: Differential expression of Ixodes ricinus salivary gland proteins in the presence of the Borrelia burgdorferi sensu lato complex.

Cotté, Violaine / Sabatier, Laurence / Schnell, Gilles / Carmi-Leroy, Annick / Rousselle, Jean-Claude / Arsène-Ploetze, Florence / Malandrin, Laurence / Sertour, Natacha / Namane, Abdelkader / Ferquel, Elisabeth / Choumet, Valérie

Journal of proteomics

2013 Volume 96, Page(s) 29–43

Abstract: In Europe, Ixodes ricinus is the main vector of Lyme borreliosis. Their salivary glands play a critical role in the biological success of ticks. To better understand the cross-talk between Borrelia burgdorferi and tick salivary glands, we analyzed ... ...

Abstract	In Europe, Ixodes ricinus is the main vector of Lyme borreliosis. Their salivary glands play a critical role in the biological success of ticks. To better understand the cross-talk between Borrelia burgdorferi and tick salivary glands, we analyzed protein expression in the salivary glands of I. ricinus adult ticks that were infected by various strains of the B. burgdorferi sl complex. iTRAQ allowed the identification of more than 120 proteins, providing the first proteomic data pertaining to I. ricinus salivary glands. Among these proteins, only 12 were modulated in the presence of various Borrelia strains. Most of them are up-regulated and are involved in cell defense and protein synthesis and processing. Down-regulated proteins are mostly implicated in the cytoskeleton. The DIGE analysis allowed us to identify 35 proteins and showed the down-regulation of 4 proteins. All 15 proteins were not modulated by all strains. Overall, these observations showed that the presence of Borrelia in tick salivary glands is a factor of stress for the protein machinery, and also that some Borrelia strains produce a dysregulation of cytoskeletal proteins. Interestingly, a protein from Borrelia, OspA, was found in infected salivary glands. The consequence of its presence in salivary glands is discussed. Biological significance: Lyme borreliosis is still the most prevalent arthropod-borne disease in the temperate regions of the northern hemisphere. The geographical distribution of Lyme borreliosis is expanding, especially towards higher altitudes and latitudes. Human pathogenic spirochetes causing Lyme borreliosis belong to the B. burgdorferi sensu lato complex. They are extracellular pathogens transmitted to humans through the bite of Ixodes spp. ticks. The bioactive molecules present in tick saliva not only promote tick feeding, but also create an advantageous microenvironment at the tick bite site for survival and replication of Borrelia bacteria. Investigation of the tick-host-pathogen interface would provide new strategies to control tick-borne infections. We chose to analyze the interaction of several strains of the B. burgdorferi sensu lato complex with I. ricinus salivary glands. We also investigated the presence of bacterial proteins in salivary glands. For these purposes, we undertook a proteomic study implying the complementary approaches of iTRAQ and DIGE. Our study allowed identifying several salivary markers of infection that were shown to vary according to the strain. Moreover, OspA, a bacterial protein was shown to be expressed in salivary glands and may be implied in the pathogenicity of some Borrelia strains.
MeSH term(s)	Animals ; Arachnid Vectors/metabolism ; Arachnid Vectors/microbiology ; Arthropod Proteins/biosynthesis ; Borrelia burgdorferi Group ; Female ; Gene Expression Regulation ; Humans ; Ixodes/metabolism ; Ixodes/microbiology ; Lyme Disease/metabolism ; Lyme Disease/transmission ; Mice ; Salivary Glands/metabolism ; Salivary Glands/microbiology ; Salivary Proteins and Peptides/biosynthesis
Chemical Substances	Arthropod Proteins ; Salivary Proteins and Peptides
Language	English
Publishing date	2013-11-02
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2400835-7
ISSN	1876-7737 ; 1874-3919
ISSN (online)	1876-7737
ISSN	1874-3919
DOI	10.1016/j.jprot.2013.10.033
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Article: The salivary glands and saliva of Anopheles gambiae as an essential step in the Plasmodium life cycle: a global proteomic study.

Choumet, Valérie / Carmi-Leroy, Annick / Laurent, Christine / Lenormand, Pascal / Rousselle, Jean-Claude / Namane, Abdelkader / Roth, Charles / Brey, Paul T

Proteomics

2007 Volume 7, Issue 18, Page(s) 3384–3394

Abstract: Proteins synthesized in the salivary glands of the Anopheles gambiae mosquito are thought to be important in the life cycle of the malaria parasite Plasmodium. To describe A. gambiae salivary gland and saliva contents, we combined several techniques: 1- ... ...

Abstract	Proteins synthesized in the salivary glands of the Anopheles gambiae mosquito are thought to be important in the life cycle of the malaria parasite Plasmodium. To describe A. gambiae salivary gland and saliva contents, we combined several techniques: 1-DE, 2-DE and LC MS/MS. This study has identified five saliva proteins and 122 more proteins from the salivary glands, including the first proteomic description for 89 of these salivary gland proteins. Since the invasion and sporozoite maturation take place during the process of salivary glands ageing, the effect of salivary gland age on salivary component composition was examined. LC MS/MS profiling of young versus old salivary gland proteomes suggests that there is an over-representation of proteins involved in signaling and proteins related to the immune response in the proteins from older mosquitoes. The iTRAQ labeling was used for a comparative proteomic analysis of salivary gland samples from infected or Plasmodium berghei-free mosquitoes. The expression levels of five secreted proteins were altered when the parasite was present. These observations will serve as a basis for future work concerning the possible role of these proteins in the interaction between A. gambiae, Plasmodium and the mammalian host.
MeSH term(s)	Animals ; Anopheles/parasitology ; Chromatography, Gel ; Electrophoresis, Polyacrylamide Gel ; Female ; Plasmodium berghei/metabolism ; Plasmodium berghei/physiology ; Proteome ; Saliva/metabolism ; Salivary Glands/metabolism ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Chemical Substances	Proteome
Language	English
Publishing date	2007-09
Publishing country	Germany
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2032093-0
ISSN	1615-9861 ; 1615-9853
ISSN (online)	1615-9861
ISSN	1615-9853
DOI	10.1002/pmic.200700334
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Zs.A 5386: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (2.OG) ab Jg. 2022: Lesesaal (EG)
Zs.A 1868: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)

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