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  1. Article ; Online: The three-dimensional structure of the EBV genome plays a crucial role in regulating viral gene expression in EBVaGC.

    Maestri, Davide / Napoletani, Giorgia / Kossenkov, Andrew / Preston-Alp, Sarah / Caruso, Lisa B / Tempera, Italo

    Nucleic acids research

    2023  Volume 51, Issue 22, Page(s) 12092–12110

    Abstract: Epstein-Barr virus (EBV) establishes lifelong asymptomatic infection by replication of its chromatinized episomes with the host genome. EBV exhibits different latency-associated transcriptional repertoires, each with distinct three-dimensional structures. ...

    Abstract Epstein-Barr virus (EBV) establishes lifelong asymptomatic infection by replication of its chromatinized episomes with the host genome. EBV exhibits different latency-associated transcriptional repertoires, each with distinct three-dimensional structures. CTCF, Cohesin and PARP1 are involved in maintaining viral latency and establishing episome architecture. Epstein-Barr virus-associated gastric cancer (EBVaGC) represents 1.3-30.9% of all gastric cancers globally. EBV-positive gastric cancers exhibit an intermediate viral transcription profile known as 'Latency II', expressing specific viral genes and noncoding RNAs. In this study, we investigated the impact of PARP1 inhibition on CTCF/Cohesin binding in Type II latency. We observed destabilization of the binding of both factors, leading to a disrupted three-dimensional architecture of the episomes and an altered viral gene expression. Despite sharing the same CTCF binding profile, Type I, II and III latencies exhibit different 3D structures that correlate with variations in viral gene expression. Additionally, our analysis of H3K27ac-enriched interactions revealed differences between Type II latency episomes and a link to cellular transformation through docking of the EBV genome at specific sites of the Human genome, thus promoting oncogene expression. Overall, this work provides insights into the role of PARP1 in maintaining active latency and novel mechanisms of EBV-induced cellular transformation.
    MeSH term(s) Humans ; Epstein-Barr Virus Infections/virology ; Gene Expression ; Genome, Viral ; Herpesvirus 4, Human/genetics ; Stomach Neoplasms/genetics ; Stomach Neoplasms/virology ; Virus Latency/genetics ; Gene Expression Regulation, Viral
    Language English
    Publishing date 2023-10-27
    Publishing country England
    Document type Journal Article
    ZDB-ID 186809-3
    ISSN 1362-4962 ; 1362-4954 ; 0301-5610 ; 0305-1048
    ISSN (online) 1362-4962 ; 1362-4954
    ISSN 0301-5610 ; 0305-1048
    DOI 10.1093/nar/gkad936
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1067: Show issues Location:
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  2. Article ; Online: Reply to Comment on COVID-19 Deaths in Long Term Care Facilities - A Critical Piece of the Puzzle.

    Lau-Ng, Rossana / Caruso, Lisa B / Perls, Thomas T

    Journal of the American Geriatrics Society

    2020  Volume 68, Issue 12, Page(s) 2748

    Abstract: This letter comments on the letter by Tak-kwan Kong. ...

    Abstract This letter comments on the letter by Tak-kwan Kong.
    MeSH term(s) COVID-19 ; Health Facilities ; Humans ; Long-Term Care ; Pandemics ; SARS-CoV-2
    Keywords covid19
    Language English
    Publishing date 2020-10-02
    Publishing country United States
    Document type Letter ; Comment
    ZDB-ID 80363-7
    ISSN 1532-5415 ; 0002-8614
    ISSN (online) 1532-5415
    ISSN 0002-8614
    DOI 10.1111/jgs.16804
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  3. Article ; Online: COVID-19 Deaths in Long-Term Care Facilities: A Critical Piece of the Pandemic Puzzle.

    Lau-Ng, Rossana / Caruso, Lisa B / Perls, Thomas T

    Journal of the American Geriatrics Society

    2020  Volume 68, Issue 9, Page(s) 1895–1898

    MeSH term(s) Aged ; COVID-19/epidemiology ; COVID-19/mortality ; Global Health/statistics & numerical data ; Health Facilities/statistics & numerical data ; Humans ; Long-Term Care ; Pandemics ; United States/epidemiology
    Keywords covid19
    Language English
    Publishing date 2020-07-20
    Publishing country United States
    Document type Editorial
    ZDB-ID 80363-7
    ISSN 1532-5415 ; 0002-8614
    ISSN (online) 1532-5415
    ISSN 0002-8614
    DOI 10.1111/jgs.16669
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  4. Article ; Online: Human Cytomegalovirus Utilizes Multiple Viral Proteins to Regulate the Basement Membrane Protein Nidogen 1.

    Kuan, Man I / Caruso, Lisa B / Zavala, Anamaria G / Rana, Pranav S J B / O'Dowd, John M / Tempera, Italo / Fortunato, Elizabeth A

    Journal of virology

    2022  Volume 96, Issue 20, Page(s) e0133622

    Abstract: Nidogen 1 (NID1) is an important basement membrane protein secreted by many cell types. We previously found that human cytomegalovirus (HCMV) infection rapidly induced chromosome 1 breaks and that the basement membrane protein NID1, encoded near the 1q42 ...

    Abstract Nidogen 1 (NID1) is an important basement membrane protein secreted by many cell types. We previously found that human cytomegalovirus (HCMV) infection rapidly induced chromosome 1 breaks and that the basement membrane protein NID1, encoded near the 1q42 break site, was downregulated. We have now determined that the specific breaks in and of themselves did not regulate NID1, rather interactions between several viral proteins and the cellular machinery and DNA regulated NID1. We screened a battery of viral proteins present by 24 hours postinfection (hpi) when regulation was induced, including components of the incoming virion and immediate early (IE) proteins. Adenovirus (Ad) delivery of the tegument proteins pp71 and UL35 and the IE protein IE1 influenced steady-state (ss) NID1 levels. IE1's mechanism of regulation was unclear, while UL35 influenced proteasomal regulation of ss NID1. Real-time quantitative PCR (RT-qPCR) experiments determined that pp71 downregulated
    MeSH term(s) Humans ; Cytomegalovirus/physiology ; Viral Proteins/metabolism ; CCCTC-Binding Factor/genetics ; Gene Expression Regulation, Viral ; Immediate-Early Proteins/genetics ; Immediate-Early Proteins/metabolism ; Basement Membrane/metabolism
    Chemical Substances Viral Proteins ; nidogen ; CCCTC-Binding Factor ; Immediate-Early Proteins
    Language English
    Publishing date 2022-10-11
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, N.I.H., Extramural
    ZDB-ID 80174-4
    ISSN 1098-5514 ; 0022-538X
    ISSN (online) 1098-5514
    ISSN 0022-538X
    DOI 10.1128/jvi.01336-22
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 609: Show issues Location:
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    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
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  5. Article: Reply to Comment on COVID-19 Deaths in Long Term Care Facilities - A Critical Piece of the Puzzle

    Lau-Ng, Rossana / Caruso, Lisa B / Perls, Thomas T

    J. am. geriatr. soc

    Abstract: This letter comments on the letter by Tak-kwan Kong. ...

    Abstract This letter comments on the letter by Tak-kwan Kong.
    Keywords covid19
    Publisher WHO
    Document type Article
    Note WHO #Covidence: #727128
    Database COVID19

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  7. Article ; Online: Reply to Comment on COVID ‐19 Deaths in Long Term Care Facilities ‐ A Critical Piece of the Puzzle

    Lau‐Ng, Rossana / Caruso, Lisa B. / Perls, Thomas T.

    Journal of the American Geriatrics Society ; ISSN 0002-8614 1532-5415

    2020  

    Keywords Geriatrics and Gerontology ; covid19
    Language English
    Publisher Wiley
    Publishing country us
    Document type Article ; Online
    DOI 10.1111/jgs.16804
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  8. Article ; Online: COVID ‐19 Deaths in Long‐Term Care Facilities

    Lau‐Ng, Rossana / Caruso, Lisa B. / Perls, Thomas T.

    Journal of the American Geriatrics Society

    A Critical Piece of the Pandemic Puzzle

    2020  Volume 68, Issue 9, Page(s) 1895–1898

    Keywords Geriatrics and Gerontology ; covid19
    Language English
    Publisher Wiley
    Publishing country us
    Document type Article ; Online
    ZDB-ID 80363-7
    ISSN 1532-5415 ; 0002-8614
    ISSN (online) 1532-5415
    ISSN 0002-8614
    DOI 10.1111/jgs.16669
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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    Ud II Zs.168: Show issues Location:
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  9. Article ; Online: Poly(ADP-ribose) polymerase 1 is necessary for coactivating hypoxia-inducible factor-1-dependent gene expression by Epstein-Barr virus latent membrane protein 1.

    Hulse, Michael / Caruso, Lisa B / Madzo, Jozef / Tan, Yinfei / Johnson, Sarah / Tempera, Italo

    PLoS pathogens

    2018  Volume 14, Issue 11, Page(s) e1007394

    Abstract: Latent membrane protein 1 (LMP1) is the major transforming protein of Epstein-Barr virus (EBV) and is critical for EBV-induced B-cell transformation in vitro. Poly(ADP-ribose) polymerase 1 (PARP1) regulates accessibility of chromatin, alters functions of ...

    Abstract Latent membrane protein 1 (LMP1) is the major transforming protein of Epstein-Barr virus (EBV) and is critical for EBV-induced B-cell transformation in vitro. Poly(ADP-ribose) polymerase 1 (PARP1) regulates accessibility of chromatin, alters functions of transcriptional activators and repressors, and has been directly implicated in transcriptional activation. Previously we showed that LMP1 activates PARP1 and increases Poly(ADP-ribos)ylation (PARylation) through PARP1. Therefore, to identify targets of LMP1 that are regulated through PARP1, LMP1 was ectopically expressed in an EBV-negative Burkitt's lymphoma cell line. These LMP1-expressing cells were then treated with the PARP inhibitor olaparib and prepared for RNA sequencing. The LMP1/PARP targets identified through this RNA-seq experiment are largely involved in metabolism and signaling. Interestingly, Ingenuity Pathway Analysis of RNA-seq data suggests that hypoxia-inducible factor 1-alpha (HIF-1α) is an LMP1 target mediated through PARP1. PARP1 is acting as a coactivator of HIF-1α-dependent gene expression in B cells, and this co-activation is enhanced by LMP1-mediated activation of PARP1. HIF-1α forms a PARylated complex with PARP1 and both HIF-1α and PARP1 are present at promoter regions of HIF-1α downstream targets, leading to accumulation of positive histone marks at these regions. Complex formation, PARylation and binding of PARP1 and HIF-1α at promoter regions of HIF-1α downstream targets can all be attenuated by PARP1 inhibition, subsequently leading to a buildup of repressive histone marks and loss of positive histone marks. In addition, LMP1 switches cells to a glycolytic 'Warburg' metabolism, preferentially using aerobic glycolysis over mitochondrial respiration. Finally, LMP1+ cells are more sensitive to PARP1 inhibition and, therefore, targeting PARP1 activity may be an effective treatment for LMP1+ EBV-associated malignancies.
    MeSH term(s) B-Lymphocytes/virology ; Cell Line, Tumor ; Gene Expression Regulation, Viral ; Herpesvirus 4, Human/genetics ; Herpesvirus 4, Human/metabolism ; Host-Pathogen Interactions ; Humans ; Hypoxia-Inducible Factor 1/genetics ; Hypoxia-Inducible Factor 1/metabolism ; Hypoxia-Inducible Factor 1, alpha Subunit/metabolism ; Phthalazines/pharmacology ; Piperazines/pharmacology ; Poly (ADP-Ribose) Polymerase-1/antagonists & inhibitors ; Poly (ADP-Ribose) Polymerase-1/metabolism ; Signal Transduction ; Transcriptional Activation ; Viral Matrix Proteins/genetics ; Viral Matrix Proteins/metabolism
    Chemical Substances EBV-associated membrane antigen, Epstein-Barr virus ; Hypoxia-Inducible Factor 1 ; Hypoxia-Inducible Factor 1, alpha Subunit ; Phthalazines ; Piperazines ; Viral Matrix Proteins ; PARP1 protein, human (EC 2.4.2.30) ; Poly (ADP-Ribose) Polymerase-1 (EC 2.4.2.30) ; olaparib (WOH1JD9AR8)
    Language English
    Publishing date 2018-11-05
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 2205412-1
    ISSN 1553-7374 ; 1553-7366
    ISSN (online) 1553-7374
    ISSN 1553-7366
    DOI 10.1371/journal.ppat.1007394
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  10. Article: Following up on clinical recommendations in transitions from hospital to nursing home.

    Caruso, Lisa B / Thwin, Soe Soe / Brandeis, Gary H

    Journal of aging research

    2014  Volume 2014, Page(s) 873043

    Abstract: Following up on recommendations made at the time of a hospital discharge is important to patient safety. While data is lacking, specifically around the transition of patient to nursing home, it has been postulated that missed items such as laboratory ... ...

    Abstract Following up on recommendations made at the time of a hospital discharge is important to patient safety. While data is lacking, specifically around the transition of patient to nursing home, it has been postulated that missed items such as laboratory tests may result in adverse patient outcomes. To determine the extent of this problem, a retrospective cohort study of subjects discharged from an academic medical center and admitted to nursing homes (NH) was followed to determine the type of discharge recommendations and the rate of completion. In addition, for the purpose of generalizability, the 30-day hospital readmission rate was calculated. 152 recommendations were made on 51 subjects. Almost a quarter of the recommendations made by the hospital discharging team were not acted upon. Furthermore, for the majority of those recommendations that were not acted upon, a reason could not be determined. In concert with national data, 20% of the subjects returned to the hospital within 30 days. Further investigation is warranted to determine if an association exists between missed recommendations and hospital readmission from the nursing home setting.
    Language English
    Publishing date 2014-02-09
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2573906-2
    ISSN 2090-2212 ; 2090-2204
    ISSN (online) 2090-2212
    ISSN 2090-2204
    DOI 10.1155/2014/873043
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