LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 4 of total 4

Search options

  1. Article ; Online: High-Density Autologous Chondrocyte Implantation as Treatment for Ankle Osteochondral Defects.

    López-Alcorocho, Juan Manuel / Guillén-Vicente, Isabel / Rodríguez-Iñigo, Elena / Navarro, Ramón / Caballero-Santos, Rosa / Guillén-Vicente, Marta / Casqueiro, Mercedes / Fernández-Jaén, Tomás F / Sanz, Fernando / Arauz, Santiago / Abelow, Steve / Guillén-García, Pedro

    Cartilage

    2019  Volume 12, Issue 3, Page(s) 307–319

    Abstract: Purpose: Two-year follow-up to assess efficacy and safety of high-density autologous chondrocyte implantation (HD-ACI) in patients with cartilage lesions in the ankle.: Design: Twenty-four consecutive patients with International Cartilage repair ... ...

    Abstract Purpose: Two-year follow-up to assess efficacy and safety of high-density autologous chondrocyte implantation (HD-ACI) in patients with cartilage lesions in the ankle.
    Design: Twenty-four consecutive patients with International Cartilage repair Society (ICRS) grade 3-4 cartilage lesions of the ankle were included. Five million chondrocytes per cm
    Results: Patients' median age was 31 years (range 18-55 years). Median VAS score was 8 (range 5-10) at baseline, 1.5 (range 0-8) at 12-month follow-up, and 2 (rang e0-5) at 24-month follow-up (
    Conclusion: HD-ACI is a safe and effective technique to treat osteochondral lesions in the talus, providing good clinical and histological results at short- and mid-term follow-ups.
    MeSH term(s) Adolescent ; Adult ; Ankle ; Ankle Joint/surgery ; Chondrocytes ; Humans ; Intra-Articular Fractures ; Middle Aged ; Talus ; Transplantation, Autologous ; Young Adult
    Language English
    Publishing date 2019-03-17
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2515870-3
    ISSN 1947-6043 ; 1947-6035
    ISSN (online) 1947-6043
    ISSN 1947-6035
    DOI 10.1177/1947603519835898
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  2. Article ; Online: Study of Telomere Length in Preimplanted Cultured Chondrocytes.

    López-Alcorocho, Juan Manuel / Guillén-Vicente, Isabel / Rodríguez-Iñigo, Elena / Guillén-Vicente, Marta / Fernández-Jaén, Tomás Fernando / Caballero, Rosa / Casqueiro, Mercedes / Najarro, Pilar / Abelow, Steve / Guillén-García, Pedro

    Cartilage

    2018  Volume 10, Issue 1, Page(s) 36–42

    Abstract: Design: In the process of cell division, the extremes of the eukaryotic chromosomes are progressively shortening, and this phenomenon is related to cell degeneration and senescence. The treatment of cartilage lesions with autologous chondrocytes implies ...

    Abstract Design: In the process of cell division, the extremes of the eukaryotic chromosomes are progressively shortening, and this phenomenon is related to cell degeneration and senescence. The treatment of cartilage lesions with autologous chondrocytes implies that cells proliferate in an artificial environment. We have studied the viability of cultured chondrocytes after measurement of their telomere length before implantation.
    Methods: Articular cartilage biopsies (B1, B2, and B3) were obtained from 3 patients (2 males and 1 female) with knee cartilage defects, who were going to be treated with chondrocyte implantation. Chondrocytes were cultured in DMEM with autologous serum. After the third passage, an aliquot of 1 million cells was removed to estimate the telomere length and the remaining cells were implanted. Telomere length was measured by quantitative fluorescent in situ hybridization (Q-FISH). Patients' clinical outcome was determined preoperatively, and 12 and 24 months postimplantation with the International Knee Documentation Committee (IKDC) questionnaire.
    Results: After chondrocyte implantation, IKDC score doubled at 12 and 24 months with regard to the basal value. After 3 passages, chondrocytes were cultured for a mean of 45.67 days, the mean duplication time being 4.53 days and the mean number of cell divisions being 10.04 during the culture period. The 20th percentile of telomere lengths were 6.84, 6.96, and 7.06 kbp and the median telomere lengths 10.30, 10.47, and 10.73 kbp, respectively. No significant correlation was found between IKDC score and telomere length.
    Conclusion: Culturing autologous chondrocytes for implantation is not related to cell senescence in terms of telomere length.
    MeSH term(s) Adult ; Cartilage Diseases/pathology ; Cartilage Diseases/therapy ; Cartilage, Articular/cytology ; Cartilage, Articular/pathology ; Cells, Cultured ; Chondrocytes/pathology ; Female ; Humans ; In Situ Hybridization, Fluorescence ; Knee Joint/cytology ; Knee Joint/pathology ; Male ; Stem Cell Transplantation ; Telomere/pathology ; Transplantation, Autologous
    Language English
    Publishing date 2018-01-11
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2515870-3
    ISSN 1947-6043 ; 1947-6035
    ISSN (online) 1947-6043
    ISSN 1947-6035
    DOI 10.1177/1947603517749918
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  3. Article: Detecting minimal traces of DNA using DNA covalently attached to superparamagnetic nanoparticles and direct PCR-ELISA.

    Fuentes, Manuel / Mateo, Cesar / Rodriguez, Ana / Casqueiro, Mercedes / Tercero, Juan C / Riese, Hans H / Fernández-Lafuente, Roberto / Guisán, Jose M

    Biosensors & bioelectronics

    2006  Volume 21, Issue 8, Page(s) 1574–1580

    Abstract: A single bond covalent immobilization of aminated DNA probes on magnetic particles suitable for selective molecular hybridization of traces of DNA samples has been developed. Commercial superparamagnetic nanoparticles containing amino groups were ... ...

    Abstract A single bond covalent immobilization of aminated DNA probes on magnetic particles suitable for selective molecular hybridization of traces of DNA samples has been developed. Commercial superparamagnetic nanoparticles containing amino groups were activated by coating with a hetero-functional polymer (aldehyde-aspartic-dextran). This new immobilization procedure provides many practical advantages: (a) DNA probes are immobilized far from the support surface preventing steric hindrances; (b) the surface of the nanoparticles cannot adsorb DNA ionically; (c) DNA probes are bound via a very strong covalent bond (a secondary amine) providing very stable immobilized probes (at 100 degrees C, or in 70% formamide, or 0.1N NaOH). Due to the extreme sensitivity of this purification procedure based on DNA hybridization, the detection of hybridized products could be coupled to a PCR-ELISA direct amplification of the DNA bond to the magnetic nanoparticles. As a model system, an aminated DNA probe specific for detecting Hepatitis C Virus cDNA was immobilized according to the optimised procedure described herein. Superparamagnetic nanoparticles containing the immobilized HCV probe were able to give a positive result after PCR-ELISA detection when hybridized with 1 mL of solution containing 10(-18) g/mL of HCV cDNA (two molecules of HCV cDNA). In addition, the detection of HCV cDNA was not impaired by the addition to the sample solution of 2.5 million-fold excess of non-complementary DNA. The experimental data supports the use of magnetic nanoparticles containing DNA probes immobilized by the procedure here described as a convenient and extremely sensitive procedure for purification/detection DNA/RNA from biological samples. The concentration/purification potential of the magnetic nanoparticles, its stability under a wide range of conditions, coupled to the possibility of using the particles directly in amplification by PCR greatly reinforces this methodology as a molecular diagnostic tool.
    MeSH term(s) Binding Sites ; Biosensing Techniques/methods ; DNA/analysis ; DNA/chemistry ; Enzyme-Linked Immunosorbent Assay/methods ; Magnetics ; Microchemistry/methods ; Molecular Probe Techniques ; Nanostructures/chemistry ; Oligonucleotide Array Sequence Analysis/methods ; Polymerase Chain Reaction/methods
    Chemical Substances DNA (9007-49-2)
    Language English
    Publishing date 2006-02-15
    Publishing country England
    Document type Evaluation Studies ; Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1011023-9
    ISSN 1873-4235 ; 0956-5663
    ISSN (online) 1873-4235
    ISSN 0956-5663
    DOI 10.1016/j.bios.2005.07.017
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 2275: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  4. Article ; Online: m.6267G>A: a recurrent mutation in the human mitochondrial DNA that reduces cytochrome c oxidase activity and is associated with tumors.

    Gallardo, M Esther / Moreno-Loshuertos, Raquel / López, Celia / Casqueiro, Mercedes / Silva, Javier / Bonilla, Felix / Rodríguez de Córdoba, Santiago / Enríquez, José Antonio

    Human mutation

    2006  Volume 27, Issue 6, Page(s) 575–582

    Abstract: Complete sequencing of the mitochondrial genome of 13 cell lines derived from a variety of human cancers revealed nine novel mitochondrial DNA (mtDNA) variations. One of them, m.6267G>A, is a recurrent mutation that introduces the Ala122Thr substitution ... ...

    Abstract Complete sequencing of the mitochondrial genome of 13 cell lines derived from a variety of human cancers revealed nine novel mitochondrial DNA (mtDNA) variations. One of them, m.6267G>A, is a recurrent mutation that introduces the Ala122Thr substitution in the mitochondrially encoded cytochrome c oxidase I (MT-CO1): p.MT-CO1: Ala122Thr (GenBank: NP_536845.1). Biochemical analysis of the original cell lines and the transmitochondrial cybrids generated by transferring mitochondrial DNAs to a common nuclear background, indicate that cytochrome c oxidase (COX) activity, respiration, and growth in galactose are impaired by the m.6267G>A mutation. This mutation, found twice in the cancer cell lines included in this study, has been also encountered in one out of 63 breast cancer samples, one out of 64 colon cancer samples, one out of 260 prostate cancer samples, and in one out of 15 pancreatic cancer cell lines. In all instances the m.6267G>A mutation was associated to different mtDNA haplogroups. These findings, contrast with the extremely low frequency of the m.6267G>A mutation in the normal population (1:2264) and its apparent absence in other pathologies, strongly suggesting that the m.6267G>A missense mutation is a recurrent mutation specifically associated with cancer.
    MeSH term(s) Amino Acid Sequence ; Cell Line, Tumor ; Conserved Sequence ; Culture Media ; DNA Mutational Analysis ; DNA, Mitochondrial/genetics ; Electron Transport Complex IV/chemistry ; Electron Transport Complex IV/genetics ; Electron Transport Complex IV/metabolism ; Galactose/pharmacology ; Humans ; Molecular Sequence Data ; Neoplasms/enzymology ; Neoplasms/genetics ; Oxygen Consumption ; Polymorphism, Restriction Fragment Length
    Chemical Substances Culture Media ; DNA, Mitochondrial ; Electron Transport Complex IV (EC 1.9.3.1) ; cytochrome c oxidase subunit I, human (EC 1.9.3.1) ; Galactose (X2RN3Q8DNE)
    Language English
    Publishing date 2006-06
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1126646-6
    ISSN 1098-1004 ; 1059-7794
    ISSN (online) 1098-1004
    ISSN 1059-7794
    DOI 10.1002/humu.20338
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 3586: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

To top