Article ; Online: High-Throughput Protein Crystallization in an Integrated Droplet-Based Microfluidic Platform.
Methods in molecular biology (Clifton, N.J.)
2023 Volume 2652, Page(s) 347–359
Abstract: We present an integrated droplet-based microfluidic platform for high-throughput protein crystallization experimentation. The device consists of commercially available micro-junctions and PFA tubing assembled for different functions: droplet generation, ... ...
Abstract | We present an integrated droplet-based microfluidic platform for high-throughput protein crystallization experimentation. The device consists of commercially available micro-junctions and PFA tubing assembled for different functions: droplet generation, incubation, and observation. Herein, we describe a complete methodology to generate hundreds of droplets with controlled properties (i.e., size, generation frequency, and composition). Therefore, multiple trials can be carried out under controlled experimental conditions for the screening and optimization of protein crystallization conditions. |
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MeSH term(s) | Microfluidics/methods ; Crystallization/methods ; Microfluidic Analytical Techniques ; Proteins/chemistry |
Chemical Substances | Proteins |
Language | English |
Publishing date | 2023-04-24 |
Publishing country | United States |
Document type | Journal Article ; Research Support, Non-U.S. Gov't |
ISSN | 1940-6029 |
ISSN (online) | 1940-6029 |
DOI | 10.1007/978-1-0716-3147-8_20 |
Database | MEDical Literature Analysis and Retrieval System OnLINE |
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