Article: Monoclonal antibodies to SARS-associated coronavirus (SARS-CoV): identification of neutralizing and antibodies reactive to S, N, M and E viral proteins.
Journal of virological methods
2004 Volume 128, Issue 1-2, Page(s) 21–28
Abstract: Monoclonal antibodies (Mabs) against the Urbani strain of the SARS-associated coronavirus (SARS-CoV) were developed and characterized for reactivity to SARS-CoV and SARS-CoV S, N, M, and E proteins using enzyme-linked immunoabsorbent (ELISA), ... ...
| Abstract | Monoclonal antibodies (Mabs) against the Urbani strain of the SARS-associated coronavirus (SARS-CoV) were developed and characterized for reactivity to SARS-CoV and SARS-CoV S, N, M, and E proteins using enzyme-linked immunoabsorbent (ELISA), radioimmunoprecipitation, immunofluorescence, Western Blot and microneutralization assays. Twenty-six mAbs were reactive to SARS-CoV by ELISA, and nine were chosen for detailed characterization. Five mAbs reacted against the S protein, two against the M protein, and one each against the N and E proteins. Two of five S protein mAbs neutralized SARS-CoV infection of Vero E6 cells and reacted to an epitope within amino acids 490-510 in the S protein. While two of the three non-neutralizing antibodies recognized at second epitope within amino acids 270-350. The mAbs characterized should prove useful for developing SARS-CoV diagnostic assays and for studying the biology of infection and pathogenesis of disease. |
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| MeSH term(s) | Animals ; Antibodies, Monoclonal/biosynthesis ; Antibodies, Monoclonal/immunology ; Antibodies, Viral/biosynthesis ; Antibodies, Viral/immunology ; Antibody Specificity ; Cell Line ; Chlorocebus aethiops ; Epitope Mapping ; Humans ; Membrane Glycoproteins/immunology ; Neutralization Tests ; Nucleocapsid Proteins/immunology ; SARS Virus/immunology ; Severe Acute Respiratory Syndrome/virology ; Spike Glycoprotein, Coronavirus ; Vero Cells ; Viral Envelope Proteins/immunology ; Viral Matrix Proteins/immunology ; Viral Structural Proteins/immunology | |||||
| Chemical Substances | Antibodies, Monoclonal ; Antibodies, Viral ; E protein, SARS coronavirus ; M protein, Coronavirus ; MHV surface projection glycoprotein ; Membrane Glycoproteins ; Nucleocapsid Proteins ; Spike Glycoprotein, Coronavirus ; Viral Envelope Proteins ; Viral Matrix Proteins ; Viral Structural Proteins ; nucleocapsid protein, Coronavirus ; spike glycoprotein, SARS-CoV | |||||
| Keywords | covid19 | |||||
| Language | English | |||||
| Publishing date | 2004-01-29 | |||||
| Publishing country | Netherlands | |||||
| Document type | Evaluation Study ; Journal Article | |||||
| ZDB-ID | 8013-5 | |||||
| ISSN | 1879-0984 ; 0166-0934 | |||||
| ISSN (online) | 1879-0984 | |||||
| ISSN | 0166-0934 | |||||
| DOI | 10.1016/j.jviromet.2005.03.021 | |||||
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| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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