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Article ; Online: Espectro antimicrobiano de dalbavancina. Mecanismo de acción y actividad in vitro frente a microorganismos Gram positivos.

Cercenado, Emilia

Enfermedades infecciosas y microbiologia clinica

2017 Volume 35 Suppl 1, Page(s) 9–14

Abstract: Because of the increase in bacterial resistance, there is a need for new antimicrobial agents. Dalbavancin is a semisynthetic glycopeptide that inhibits the late stages of bacterial cell wall synthesis in the same way as vancomycin, but in addition, its ... ...

Title translation	Antimicrobial spectrum of dalbavancin. Mechanism of action and in vitro activity against Gram-positive microorganisms.
Abstract	Because of the increase in bacterial resistance, there is a need for new antimicrobial agents. Dalbavancin is a semisynthetic glycopeptide that inhibits the late stages of bacterial cell wall synthesis in the same way as vancomycin, but in addition, its lipophilic side chain anchors dalbavancin to the cellular membrane and allows enhanced activity compared with that of vancomycin. Dalbavancin possesses a broad spectrum of in vitro activity against Gram-positive aerobic and anaerobic microorganisms, being 4-8 times more potent than vancomycin. The spectrum of dalbavancin includes staphylococci, enterococci, streptococci, and anaerobic Gram-positive cocci and bacilli. It is active against different species of multiresistant microorganisms, including methicillin-resistant Staphylococcus aureus and penicillin-resistant viridans streptococci and Streptococcus pneumoniae. Although it shows in vitro activity against Enterococcus spp., it is inactive against isolates expressing the VanA phenotype of vancomycin resistance. It also shows slow bactericidal activity against S. aureus, coagulase-negative staphylococci, and Streptococcus pyogenes. In general, the MIC
Language	Spanish
Publishing date	2017-01
Publishing country	Spain
Document type	English Abstract ; Journal Article
ZDB-ID	1070941-1
ISSN	1578-1852 ; 0213-005X
ISSN (online)	1578-1852
ISSN	0213-005X
DOI	10.1016/S0213-005X(17)30029-0
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Article ; Online: Invasive disease caused simultaneously by two different serotypes of Streptococcus pneumoniae: a microbiological appreciation.

Cercenado, Emilia / Ramos, Belén / Pérez-Abeledo, Marta / Sempere, Julio / Yuste, Jose / Sanz, Juan Carlos

European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology

2024 Volume 43, Issue 5, Page(s) 1013–1016

Abstract: We report a clinical case of a child with an invasive pneumococcal disease caused by two different pneumococcal serotypes that belonged to different sequence types. She was a 15-month-old girl with pneumonia and pleural effusion in which S. pneumoniae ... ...

Abstract	We report a clinical case of a child with an invasive pneumococcal disease caused by two different pneumococcal serotypes that belonged to different sequence types. She was a 15-month-old girl with pneumonia and pleural effusion in which S. pneumoniae colonies with different morphologies grew, one from the blood culture (characteristic greyish appearance) and the other from the pleural fluid (mucoid appearance). The isolate from blood was serotype 22 F (ST698/CC698/GPSC61), while the isolate from the pleural fluid was serotype 3 (ST180/CC180/GPSC12). The patient fully recovered after treatment with intravenous ampicillin followed by oral amoxicillin.
MeSH term(s)	Humans ; Streptococcus pneumoniae/isolation & purification ; Streptococcus pneumoniae/classification ; Streptococcus pneumoniae/genetics ; Female ; Infant ; Serogroup ; Anti-Bacterial Agents/therapeutic use ; Pneumococcal Infections/microbiology ; Pneumococcal Infections/drug therapy ; Pneumococcal Infections/diagnosis ; Pleural Effusion/microbiology ; Amoxicillin/therapeutic use ; Ampicillin/therapeutic use ; Pneumonia, Pneumococcal/microbiology ; Pneumonia, Pneumococcal/drug therapy ; Pneumonia, Pneumococcal/diagnosis ; Treatment Outcome
Language	English
Publishing date	2024-02-28
Publishing country	Germany
Document type	Case Reports ; Journal Article
ZDB-ID	603155-9
ISSN	1435-4373 ; 0934-9723 ; 0722-2211
ISSN (online)	1435-4373
ISSN	0934-9723 ; 0722-2211
DOI	10.1007/s10096-024-04787-x
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Article ; Online: Detección de enterobacterias portadoras de carbapenemasas en la rutina del laboratorio.

Cercenado, Emilia

Revista espanola de quimioterapia : publicacion oficial de la Sociedad Espanola de Quimioterapia

2015 Volume 28 Suppl 1, Page(s) 8–11

Abstract: Detection of carbapenemase-producing Enterobacteriaceae in the laboratory requires an exhaustive analysis of the antibiogram and susceptibility to all beta-lactams, the implementation with phenotypic methods of screening as well as confirmatory ... ...

Title translation	Laboratory detection of carbapenemase-producing Enterobacteriaceae.
Abstract	Detection of carbapenemase-producing Enterobacteriaceae in the laboratory requires an exhaustive analysis of the antibiogram and susceptibility to all beta-lactams, the implementation with phenotypic methods of screening as well as confirmatory procedures including the detection of the carbapenem hydrolysis, the inhibition of the enzyme activity with several specific inhibitor compounds and by molecular methods.
MeSH term(s)	Anti-Bacterial Agents/pharmacology ; Anti-Bacterial Agents/therapeutic use ; Bacterial Proteins/metabolism ; Carbapenems/pharmacology ; Carbapenems/therapeutic use ; Drug Resistance, Bacterial ; Enterobacteriaceae/drug effects ; Enterobacteriaceae/enzymology ; Enterobacteriaceae Infections/drug therapy ; Enterobacteriaceae Infections/microbiology ; Humans ; Microbial Sensitivity Tests ; beta-Lactamases/metabolism
Chemical Substances	Anti-Bacterial Agents ; Bacterial Proteins ; Carbapenems ; beta-Lactamases (EC 3.5.2.6) ; carbapenemase (EC 3.5.2.6)
Language	Spanish
Publishing date	2015-09
Publishing country	Spain
Document type	English Abstract ; Journal Article ; Review
ZDB-ID	1018135-0
ISSN	1988-9518 ; 0214-3429
ISSN (online)	1988-9518
ISSN	0214-3429
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Article: Corrigendum: Tranexamic acid in combination with vancomycin or gentamicin has a synergistic effect against staphylococci.

Benjumea, Antonio / Díaz-Navarro, Marta / Hafian, Rama / Cercenado, Emilia / Sánchez-Somolinos, Mar / Vaquero, Javier / Chana, Francisco / Muñoz, Patricia / Guembe, María

Frontiers in microbiology

2024 Volume 15, Page(s) 1355087

Abstract: This corrects the article DOI: 10.3389/fmicb.2022.935646.]. ...

Abstract	[This corrects the article DOI: 10.3389/fmicb.2022.935646.].
Language	English
Publishing date	2024-02-02
Publishing country	Switzerland
Document type	Published Erratum
ZDB-ID	2587354-4
ISSN	1664-302X
ISSN	1664-302X
DOI	10.3389/fmicb.2024.1355087
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Article ; Online: Addressing catheter lock therapy: Does heparin reduce the bioactivity of dalbavancin when together in solution during freezing?

Díaz-Navarro, Marta / Hafian, Rama / Pérez-Granda, María Jesús / Cercenado, Emilia / Muñoz, Patricia / Guembe, María

Enfermedades infecciosas y microbiologia clinica (English ed.)

2024

Abstract: Introduction: The possible use of dalbavancin as a catheter lock solution was previously demonstrated by our study group. However, it was needed to assess whether heparin could affect dalbavancin bioactivity during freezing storage.: Methods: We ... ...

Abstract	Introduction: The possible use of dalbavancin as a catheter lock solution was previously demonstrated by our study group. However, it was needed to assess whether heparin could affect dalbavancin bioactivity during freezing storage. Methods: We tested the bioactivity of a dalbavancin+heparin (DH) vs. dalbavancin (D) against Staphylococcal biofilms comparing DH median value of cfu counts and metabolic activity with that obtained for D before and during storage under freezing up to 6 months. Results: Despite there was a slight decrease in the median percentage reduction of metabolic activity at month 3 in Staphylococcus epidermidis between DH and D (97.6 vs. 100, p=0.037), considering the clinical criteria, no significant reduction in any of the variables tested was observed at the end of the experiment between D and DH solutions. Conclusion: The addition of heparin to a dalbavancin lock solution did not affect its bioactivity against staphylococcal biofilms irrespective of its preservation time under freezing.
Language	English
Publishing date	2024-05-04
Publishing country	Spain
Document type	Journal Article
ISSN	2529-993X
ISSN (online)	2529-993X
DOI	10.1016/j.eimce.2024.02.013
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Article ; Online: Enterococcus: resistencias fenotípicas y genotípicas y epidemiología en España.

Cercenado, Emilia

Enfermedades infecciosas y microbiologia clinica

2011 Volume 29 Suppl 5, Page(s) 59–65

Abstract: Enterococci are major nosocomial pathogens due to their intrinsic resistance to many antimicrobials as well as to their ability to acquire new mechanisms of resistance. Acquired resistance to beta-lactams is due to PBP5 overproduction or alterations in ... ...

Title translation	Enterococcus: phenotype and genotype resistance and epidemiology in Spain.
Abstract	Enterococci are major nosocomial pathogens due to their intrinsic resistance to many antimicrobials as well as to their ability to acquire new mechanisms of resistance. Acquired resistance to beta-lactams is due to PBP5 overproduction or alterations in this protein. Beta-lactamase production is anecdotal. High-level resistance (HLR) to aminoglycosides is due to the production of aminoglycoside-modifying enzymes that delete synergistic killing in association with cell wall-active agents. The most frequent enzyme is AAC(6')- APH(2"), which inactivates all the aminoglycosides most frequently used in clinical practice. Acquired resistance to glycopeptides is due to the acquisition of gene clusters called vanA, vanB, vanD, vanE, vanG, vanL, vanM and vanN. Linezolid resistance is due to ribosomal mutations or to the acquisition of the cfr gene. Some isolates present diminished susceptibility to daptomycin. In Spain, both enterococcal resistance to beta-lactams and HLR to aminoglycosides are high. E. faecalis is almost uniformly susceptible to ampicillin. Enterococcal resistance to glycopeptides is low, with the exception of occasional outbreaks. The new antimicrobials (linezolid, daptomycin, tigecycline) are almost uniformly active against these microorganisms. Because of the wide dissemination of the high-risk clonal complexes CC2 and CC9 (E. faecalis), and CC17 (E. faecium), surveillance studies are required to detect antimicrobial resistance genes as well as to identify high-risk clonal complexes in order to predict future trends in the acquisition of resistance genes.
MeSH term(s)	Anti-Bacterial Agents/administration & dosage ; Anti-Bacterial Agents/classification ; Anti-Bacterial Agents/pharmacology ; Anti-Bacterial Agents/therapeutic use ; Bacterial Proteins/genetics ; Drug Resistance, Multiple, Bacterial/genetics ; Drug Resistance, Multiple, Bacterial/physiology ; Drug Therapy, Combination ; Enterococcus/drug effects ; Enterococcus/enzymology ; Enterococcus/genetics ; Genes, Bacterial ; Genotype ; Gram-Positive Bacterial Infections/drug therapy ; Gram-Positive Bacterial Infections/microbiology ; Humans ; Operon ; Phenotype ; Population Surveillance ; Spain/epidemiology ; Viral Proteins/genetics ; Viral Proteins/physiology
Chemical Substances	Anti-Bacterial Agents ; Bacterial Proteins ; Viral Proteins
Language	Spanish
Publishing date	2011-12
Publishing country	Spain
Document type	English Abstract ; Journal Article ; Review
ZDB-ID	1070941-1
ISSN	1578-1852 ; 0213-005X
ISSN (online)	1578-1852
ISSN	0213-005X
DOI	10.1016/S0213-005X(11)70045-3
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Article ; Online: In vitro activity of delafloxacin against highly levofloxacin-resistant invasive isolates of Streptococcus pneumoniae.

Cercenado, Emilia / Loras, Cristina / Cobos, Alejandro / Sanz, Juan Carlos

Enfermedades infecciosas y microbiologia clinica (English ed.)

2021 Volume 40, Issue 3, Page(s) 131–133

Abstract: Introduction: We report the activity of delafloxacin, a new fluoroquinolone with high affinity for both topoisomerase IV and DNA gyrase, against highly-levofloxacin-resistant invasive strains of Streptococcus pneumoniae.: Methods: A total of 173 ... ...

Abstract	Introduction: We report the activity of delafloxacin, a new fluoroquinolone with high affinity for both topoisomerase IV and DNA gyrase, against highly-levofloxacin-resistant invasive strains of Streptococcus pneumoniae. Methods: A total of 173 highly-levofloxacin-resistant (MIC>32mg/L) S. pneumoniae invasive isolates were studied. The strains were isolated from blood (n=162) and other sterile fluids (n=11). Serotyping was performed by the Pneumotest-Latex and Quellung reaction. Delafloxacin, levofloxacin, penicillin, cefotaxime, erythromycin and vancomycin MICs were determined by the gradient diffusion method following EUCAST guidelines and breakpoints. Results: Among the isolates, 32.9% were penicillin non-susceptible, 19.7% cefotaxime non-susceptible, and 76.9% erythromycin resistant. All were susceptible to vancomycin. Delafloxacin MIC Conclusions: Delafloxacin was very active against highly-levofloxacin-resistant invasive isolates of S. pneumoniae. Isolates belonging to serotype 9V showed higher delafloxacin MIC values.
MeSH term(s)	Anti-Bacterial Agents/pharmacology ; Fluoroquinolones/pharmacology ; Levofloxacin/pharmacology ; Streptococcus pneumoniae
Chemical Substances	Anti-Bacterial Agents ; Fluoroquinolones ; delafloxacin (6315412YVF) ; Levofloxacin (6GNT3Y5LMF)
Language	English
Publishing date	2021-12-18
Publishing country	Spain
Document type	Journal Article
ISSN	2529-993X
ISSN (online)	2529-993X
DOI	10.1016/j.eimce.2020.09.009
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Article ; Online: Actualización en las resistencias de las bacterias grampositivas.

Cercenado, Emilia

Medicina clinica

2010 Volume 135 Suppl 3, Page(s) 10–15

Abstract: In the last few decades, resistance among Gram-positive microorganisms to classical antimicrobials as well as the emergence of resistance to new antimicrobials has been observed in our environment. Methicillin resistance among Staphylococcus aureus and ... ...

Title translation	Update of antimicrobial resistance in Gram-positive microorganisms.
Abstract	In the last few decades, resistance among Gram-positive microorganisms to classical antimicrobials as well as the emergence of resistance to new antimicrobials has been observed in our environment. Methicillin resistance among Staphylococcus aureus and coagulase-negative staphylococci seems to have stabilized at around 30% and 70%, respectively, however, multiresistance of these species to other antimicrobials, emergence of linezolid resistance, and decreased susceptibility to glycopeptides is a cause of concern. Daptomycin has good antimicrobial activity, although some strains with slightly increased MICs have been detected. Among enterococci, vancomycin resistance is less than 5%, but multiresistance among these microorganisms, emerging linezolid resistance and reports of some isolates with decreased susceptibility to daptomycin are worrying. Adequate use of antimicrobials could help to prevent the increase in resistance and dissemination of these pathogens and will allow their efficacy to be guaranteed in the future.
MeSH term(s)	Acetamides/pharmacology ; Acetamides/therapeutic use ; Anti-Bacterial Agents/pharmacology ; Anti-Bacterial Agents/therapeutic use ; Community-Acquired Infections/drug therapy ; Community-Acquired Infections/epidemiology ; Community-Acquired Infections/microbiology ; Cross Infection/drug therapy ; Cross Infection/epidemiology ; Cross Infection/microbiology ; Daptomycin/pharmacology ; Daptomycin/therapeutic use ; Drug Resistance, Microbial ; Drug Resistance, Multiple, Bacterial ; Enterococcus/drug effects ; Europe/epidemiology ; Gram-Positive Bacteria/drug effects ; Gram-Positive Bacterial Infections/drug therapy ; Humans ; Linezolid ; Methicillin-Resistant Staphylococcus aureus/drug effects ; Oxazolidinones/pharmacology ; Oxazolidinones/therapeutic use ; Staphylococcal Infections/drug therapy ; Staphylococcal Infections/epidemiology ; United States/epidemiology ; Vancomycin/pharmacology ; Vancomycin/therapeutic use
Chemical Substances	Acetamides ; Anti-Bacterial Agents ; Oxazolidinones ; Vancomycin (6Q205EH1VU) ; Linezolid (ISQ9I6J12J) ; Daptomycin (NWQ5N31VKK)
Language	Spanish
Publishing date	2010-12
Publishing country	Spain
Document type	English Abstract ; Journal Article ; Review
ZDB-ID	411607-0
ISSN	1578-8989 ; 0025-7753
ISSN (online)	1578-8989
ISSN	0025-7753
DOI	10.1016/S0025-7753(10)70035-X
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Article ; Online: Reporting antimicrobial susceptibilities and phenotypes of resistance to vancomycin in vancomycin-resistant Enterococcus spp. clinical isolates: A nationwide proficiency study.

Fernández-Cuenca, Felipe / López-Hernández, Inmaculada / Cercenado, Emilia / Conejo, María Carmen / Tormo, Nuria / Gimeno, Concepción / Pascual, Alvaro

Enfermedades infecciosas y microbiologia clinica (English ed.)

2023 Volume 41, Issue 6, Page(s) 335–341

Abstract: Introduction: The ability of Spanish microbiology laboratories to (a) determine antimicrobial susceptibility (AS), and (b) correctly detect the vancomycin resistance (VR) phenotype in vancomycin-resistant Enterococcus spp. (VRE) was evaluated.: ... ...

Abstract	Introduction: The ability of Spanish microbiology laboratories to (a) determine antimicrobial susceptibility (AS), and (b) correctly detect the vancomycin resistance (VR) phenotype in vancomycin-resistant Enterococcus spp. (VRE) was evaluated. Methods: Three VRE isolates representing the VanA (E. faecium), VanB (E. faecium) and VanC (E. gallinarum) VR phenotypes were sent to 52 laboratories, which were asked for: (a) AS method used; (b) MICs of ampicillin, imipenem, vancomycin, teicoplanin, linezolid, daptomycin, ciprofloxacin, levofloxacin and quinupristin-dalfopristin, and high-level resistance to gentamicin and streptomycin; (c) VR phenotype. Results: (a) The most frequently used system was MicroScan; (b) according to the system, the highest percentage of discrepant MICs was found with gradient strips (21.3%). By antimicrobial, the highest rates of discrepant MICs ranged 16.7% (imipenem) to 0.7% (linezolid). No discrepant MICs were obtained with daptomycin or levofloxacin. Mayor errors (MEs) occurred with linezolid (1.1%/EUCAST) and ciprofloxacin (5.0%/CLSI), and very major errors (VMEs) with vancomycin (27.1%/EUCAST and 33.3%/CLSI) and teicoplanin (5.7%/EUCAST and 2.3%/CLSI). For linezolid, ciprofloxacin, and vancomycin, discrepant MICs were responsible for these errors, while for teicoplanin, errors were due to a misassignment of the clinical category. An unacceptable high percentage of VMEs was obtained using gradient strips (14.8%), especially with vancomycin, teicoplanin and daptomycin; (c) 86.4% of the centers identified VanA and VanB phenotypes correctly, and 95.0% the VanC phenotype. Conclusion: Most Spanish microbiology laboratories can reliably determine AS in VRE, but there is a significant percentage of inadequate interpretations (warning of false susceptibility) for teicoplanin in isolates with the VanB phenotype.
MeSH term(s)	Vancomycin/pharmacology ; Anti-Bacterial Agents/pharmacology ; Teicoplanin/pharmacology ; Daptomycin/pharmacology ; Linezolid/pharmacology ; Levofloxacin ; Vancomycin-Resistant Enterococci/genetics ; Phenotype ; Ciprofloxacin ; Imipenem
Chemical Substances	Vancomycin (6Q205EH1VU) ; Anti-Bacterial Agents ; Teicoplanin (61036-62-2) ; Daptomycin (NWQ5N31VKK) ; Linezolid (ISQ9I6J12J) ; Levofloxacin (6GNT3Y5LMF) ; Ciprofloxacin (5E8K9I0O4U) ; Imipenem (71OTZ9ZE0A)
Language	English
Publishing date	2023-01-05
Publishing country	Spain
Document type	Journal Article
ISSN	2529-993X
ISSN (online)	2529-993X
DOI	10.1016/j.eimce.2021.11.013
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Article: Staphylococcus lugdunensis: un estafilococo coagulasa negativo diferente de los demás.

Cercenado, Emilia

Enfermedades infecciosas y microbiologia clinica

2009 Volume 27, Issue 3, Page(s) 139–142

Title translation	Staphylococcus lugdunensis: a unique coagulase-negative staphylococcus.
MeSH term(s)	Bacterial Proteins/genetics ; Drug Resistance, Multiple, Bacterial ; Genes, Bacterial ; Humans ; Opportunistic Infections/microbiology ; Quorum Sensing/genetics ; Staphylococcal Infections/microbiology ; Staphylococcus/drug effects ; Staphylococcus/pathogenicity ; Staphylococcus/physiology ; Virulence
Chemical Substances	Bacterial Proteins
Language	Spanish
Publishing date	2009-03
Publishing country	Spain
Document type	Editorial
ZDB-ID	1070941-1
ISSN	1578-1852 ; 0213-005X
ISSN (online)	1578-1852
ISSN	0213-005X
DOI	10.1016/j.eimc.2009.01.001
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