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  1. Article: Trends in Antibiotic Resistance of Nosocomial and Community-Acquired Infections in Italy.

    Cerini, Paola / Meduri, Francesca Rita / Tomassetti, Flaminia / Polidori, Isabella / Brugneti, Marta / Nicolai, Eleonora / Bernardini, Sergio / Pieri, Massimo / Broccolo, Francesco

    Antibiotics (Basel, Switzerland)

    2023  Volume 12, Issue 4

    Abstract: The World Health Organization has recently identified three categories of pathogens, namely: critical, high, and medium priority, according to the need for new antibiotics. Critical priority pathogens include carbapenem-resistant microorganism (CPO) such ...

    Abstract The World Health Organization has recently identified three categories of pathogens, namely: critical, high, and medium priority, according to the need for new antibiotics. Critical priority pathogens include carbapenem-resistant microorganism (CPO) such as
    Language English
    Publishing date 2023-03-24
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2681345-2
    ISSN 2079-6382
    ISSN 2079-6382
    DOI 10.3390/antibiotics12040651
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Evaluation of ECLIA antigen detection tests as screening methods for COVID-19 in comparison with molecular analysis.

    Kolesova, Olga / Tomassetti, Flaminia / Cerini, Paola / Finucci, Davide / Turchetti, Giordano / Capogreco, Francesca / Bernardini, Sergio / Calugi, Graziella / Pieri, Massimo

    Irish journal of medical science

    2021  Volume 191, Issue 5, Page(s) 2213–2217

    Abstract: Background: Data from literature shows that antigen tests are rapid and helpful tools for diagnosis of COVID-19.: Aim: This work aimed to evaluate the performances of the Elecsys SARS-CoV-2 Antigen test, in comparison to RT-qPCR, the gold standard.!## ...

    Abstract Background: Data from literature shows that antigen tests are rapid and helpful tools for diagnosis of COVID-19.
    Aim: This work aimed to evaluate the performances of the Elecsys SARS-CoV-2 Antigen test, in comparison to RT-qPCR, the gold standard.
    Methods: A total of 110 swabs were tested; according to rRT-PCR, 76 were positive, and 34 were negative. The swabs were processed by Elecsys SARS CoV 2 Antigen assay (Roche Diagnostics GmbH, Mannheim, Germany), an electrochemiluminescence immunoassay (ECLIA).
    Results: In a first evaluation, the overall sensitivity and specificity were 85% and 100%, respectively. It was noted that most of the discordant cases had cycle threshold (Ct) values > 28. Therefore, it was assumed a new measure to evaluate sensitivity and specificity, then samples with Ct values < 28 were selected. In this way, it was achieved a Ct < 28 sensitivity of 94%. The level of agreement between the two tests was 89. 1% with κ value of 0.77 for total data and 95.9% with κ value of 0.95 for samples with < 28 Ct. The antigen test performs well in the presence of high viral loads, whereas lower levels are missed.
    Conclusions: The comparison data obtained in this study support that this method seems a proper approach for rapid screening of patients with high SARS-CoV-2 viral load; however, the rate of sensitivity is highly Ct-dependent.
    MeSH term(s) COVID-19/diagnosis ; COVID-19 Testing ; Humans ; RNA, Viral ; SARS-CoV-2 ; Sensitivity and Specificity
    Chemical Substances RNA, Viral
    Language English
    Publishing date 2021-11-30
    Publishing country Ireland
    Document type Journal Article
    ZDB-ID 390895-1
    ISSN 1863-4362 ; 0021-1265
    ISSN (online) 1863-4362
    ISSN 0021-1265
    DOI 10.1007/s11845-021-02863-1
    Database MEDical Literature Analysis and Retrieval System OnLINE

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