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  1. Article ; Online: Fatal biliary-systemic air embolism during endoscopic retrograde cholangiopancreatography: a case with multifocal liver abscesses and choledochoduodenostomy.

    Cha, Sung Tae / Kwon, Chang-Il / Seon, Han Gyung / Ko, Kwang Hyun / Hong, Sung Pyo / Hwang, Seong Gyu / Park, Pil Won / Rim, Kyu Sung

    Yonsei medical journal

    2010  Volume 51, Issue 2, Page(s) 287–290

    Abstract: We report a rare case of a massive fatal embolism that occurred in the middle of endoscopic retrograde cholangiopancreatography (ERCP) and retrospectively examine the significant causes of the event. The patient was a 50-year old female with an uncertain ...

    Abstract We report a rare case of a massive fatal embolism that occurred in the middle of endoscopic retrograde cholangiopancreatography (ERCP) and retrospectively examine the significant causes of the event. The patient was a 50-year old female with an uncertain history of previous abdominal surgery for multiple biliary stones 20 years prior. The patient presented with acute right upper quadrant pain. An abdominal computed tomographic (CT) scan revealed the presence of multiple stones in the common bile duct (CBD) and intra-hepatic duct (IHD) with biliary obstruction, multifocal liver abscesses, and air-biliarygram. Emergency ERCP showed a wide and straight opening of choledochoduodenostomy, which may have been created during a previous surgery, and multiple filling defects in the CBD. With the use of a forward endoscope, mud stones were extracted through the opening of the choledochoduodenostomy. Cardiac arrest suddenly developed during the procedure, and despite immediate resuscitation, the patient died due to a massive systemic air embolism. We reviewed previously reported fatal cases and accessed factors facilitating air embolisms in this case.
    MeSH term(s) Cholangiopancreatography, Endoscopic Retrograde/methods ; Choledochostomy/methods ; Common Bile Duct/diagnostic imaging ; Embolism, Air/complications ; Fatal Outcome ; Female ; Humans ; Liver Abscess/pathology ; Middle Aged ; Tomography, X-Ray Computed
    Language English
    Publishing date 2010-02-12
    Publishing country Korea (South)
    Document type Case Reports ; Journal Article
    ZDB-ID 303740-x
    ISSN 1976-2437 ; 0513-5796
    ISSN (online) 1976-2437
    ISSN 0513-5796
    DOI 10.3349/ymj.2010.51.2.287
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.B 944: Show issues Location:
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  2. Article: A method of isolation and culture of microvascular endothelial cells from mouse skin.

    Cha, Sung Tae / Talavera, Dodanim / Demir, Erhan / Nath, Anjali K / Sierra-Honigmann, M Rocio

    Microvascular research

    2005  Volume 70, Issue 3, Page(s) 198–204

    Abstract: Objectives: The study of isolated microvascular endothelial cells from mice has long been impeded due to the many difficulties encountered in isolating and culturing these cells. We focused on developing a method to isolate microvascular endothelial ... ...

    Abstract Objectives: The study of isolated microvascular endothelial cells from mice has long been impeded due to the many difficulties encountered in isolating and culturing these cells. We focused on developing a method to isolate microvascular endothelial cells from the skin fragments of newborn mice. We also aimed at establishing optimal culture conditions to sustain the growth of these cells.
    Methods and results: Isolation of murine dermal microvascular endothelial cells (mDMEC) from P3 newborn mice was based first on enzymatic separation of the skin epidermal layer from the dermis using dispase and then on disaggregating dermal cellular elements using collagenase. The cells obtained from the dermis were subjected to a continuous density gradient centrifugation. Cells situated between densities 1.033 and 1.047 were then cultured on collagen IV-coated culture flasks using optimized growth culture conditions. Cells were characterized by endothelial appearance and by the presence and genetic expression of endothelial markers like CD31, NOS3, VEGFR-2 and Tie-2. Uptake of acetylated low-density lipoprotein (Ac-LDL) was used as a functional assay.
    Conclusions: The methodology described herein for isolation and culture of murine microvascular endothelium offers a distinctive advantage for those using mouse models to study endothelial cell biology.
    MeSH term(s) Animals ; Antigens, CD ; Antigens, CD34/biosynthesis ; Cadherins/biosynthesis ; Cell Culture Techniques/methods ; Cells, Cultured ; Endothelial Cells/metabolism ; Endothelium, Vascular/cytology ; Endothelium, Vascular/metabolism ; Endothelium, Vascular/pathology ; Flow Cytometry ; Gene Expression Regulation ; Lipoproteins, LDL/metabolism ; Mice ; Microcirculation ; Microscopy, Fluorescence ; Microscopy, Phase-Contrast ; Models, Biological ; Phenotype ; Platelet Endothelial Cell Adhesion Molecule-1/biosynthesis ; RNA, Messenger/metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; Skin/metabolism ; Skin/pathology ; Vascular Endothelial Growth Factor Receptor-2/metabolism
    Chemical Substances Antigens, CD ; Antigens, CD34 ; Cadherins ; Lipoproteins, LDL ; Platelet Endothelial Cell Adhesion Molecule-1 ; RNA, Messenger ; cadherin 5 ; Vascular Endothelial Growth Factor Receptor-2 (EC 2.7.10.1)
    Language English
    Publishing date 2005-11
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 80307-8
    ISSN 1095-9319 ; 0026-2862
    ISSN (online) 1095-9319
    ISSN 0026-2862
    DOI 10.1016/j.mvr.2005.08.002
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 746: Show issues Location:
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  3. Article: Fully Endoscopic Vascular Decompression of the Facial Nerve for Hemifacial Spasm

    Eby, Joseph B. / Cha, Sung Tae / Shahinian, Hrayr K.

    Skull Base

    2001  Volume 11, Issue 03, Page(s) 189–198

    Abstract: Hemifacial spasm is an uncommon disorder manifesting as a unilateral, involuntary, sporadic contraction of the musculature innervated by the seventh cranial nerve. Although debated, the etiology of hemifacial spasm is generally accepted as compression of ...

    Abstract Hemifacial spasm is an uncommon disorder manifesting as a unilateral, involuntary, sporadic contraction of the musculature innervated by the seventh cranial nerve. Although debated, the etiology of hemifacial spasm is generally accepted as compression of the facial nerve by vessels of the posterior circulation. Early surgical techniques were ineffective and fraught with morbidity. Over the past 25 years microvascular decompression surgery has allowed the safe and effective treatment of hemifacial spasm. Recent reports combining microsurgical and endoscopic techniques have documented the advantages of the endoscope in exposing the anatomy of this region. Enhanced visualization allows a less traumatic dissection and increases the surgeon's ability to locate nerve-vessel conflicts often difficult to identify through the limited view of the microscope. This article reviews the history of hemifacial spasm and describes the first three cases of fully endoscopic vascular decompression for hemifacial spasm, emphasizing the advantages of this novel surgical approach.
    Keywords Endoscopy ; hemifacial spasm ; vascular decompression
    Language English
    Publishing date 2001-01-01
    Publishing place Stuttgart ; New York
    Document type Article
    ZDB-ID 2043690-7
    ISSN 1532-0065 ; 1531-5010
    ISSN (online) 1532-0065
    ISSN 1531-5010
    DOI 10.1055/s-2001-16607
    Database Thieme publisher's database

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  4. Article: Fully endoscopic vascular decompression of the glossopharyngeal nerve.

    Jarrahy, Reza / Cha, Sung Tae / Eby, Joseph B / Berci, George / Shahinian, Hrayr K

    The Journal of craniofacial surgery

    2002  Volume 13, Issue 1, Page(s) 90–95

    Abstract: Microvascular decompression of the glossopharyngeal nerve is an effective treatment of patients with glossopharyngeal neuralgia in whom compression of the nerve by a blood vessel is implicated in the pathogenesis of the disease. The standard surgical ... ...

    Abstract Microvascular decompression of the glossopharyngeal nerve is an effective treatment of patients with glossopharyngeal neuralgia in whom compression of the nerve by a blood vessel is implicated in the pathogenesis of the disease. The standard surgical technique uses a binocular operating microscope for intra-operative visualization. Growing experience with posterior fossa endoscopy, however, has suggested that endoscopes may provide more comprehensive anatomical views of cerebellopontine angle. This report describes the case of a patient suffering from glossopharyngeal neuralgia who underwent fully endoscopic vascular decompression of the glossopharyngeal nerve. During this procedure the endoscope was used to survey the posterior fossa, guide the placement of insulating sponges, and conduct a final assessment of the intervention. We found the endoscope ideally suited to the constricted operating space of the posterior fossa, allowing for accurate localization and careful separation of the pathological vascular conflict with minimal brain retraction and no damage to surrounding structures. The versatility of endoscopy allows for superior visual appreciation of neurovascular conflicts in the posterior fossa. To date, endoscopy has primarily been used to supplement microscopy in cranial nerve decompression surgery. This report demonstrates how the endoscope can be used as the sole imaging modality in glossopharyngeal nerve decompression, with excellent results.
    MeSH term(s) Adult ; Cranial Fossa, Posterior/surgery ; Decompression, Surgical/methods ; Endoscopy/methods ; Glossopharyngeal Nerve/surgery ; Glossopharyngeal Nerve Diseases/surgery ; Humans ; Male ; Neurosurgical Procedures/methods ; Vertebral Artery/surgery
    Language English
    Publishing date 2002-01-28
    Publishing country United States
    Document type Case Reports ; Journal Article
    ZDB-ID 1159501-2
    ISSN 1536-3732 ; 1049-2275
    ISSN (online) 1536-3732
    ISSN 1049-2275
    DOI 10.1097/00001665-200201000-00021
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 3761: Show issues Location:
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    Zs.MO 175: Show issues

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  5. Article ; Online: STAT3 expression in gastric cancer indicates a poor prognosis.

    Kim, Dae-Young / Cha, Sung-Tae / Ahn, Dae-Ho / Kang, Hae-Yoon / Kwon, Chang-Il / Ko, Kwang-Hyun / Hwang, Seong-Gyu / Park, Pil-Won / Rim, Kyu-Sung / Hong, Sung-Pyo

    Journal of gastroenterology and hepatology

    2009  Volume 24, Issue 4, Page(s) 646–651

    Abstract: Background and aim: Signal transducers and activators of transcription (STAT) behave as signal transducers in the cytoplasm and as transcription factors in the nucleus. In the current study, we analyzed the immunohistochemical staining patterns of ... ...

    Abstract Background and aim: Signal transducers and activators of transcription (STAT) behave as signal transducers in the cytoplasm and as transcription factors in the nucleus. In the current study, we analyzed the immunohistochemical staining patterns of gastrectomy tissue specimens. We investigated the expression of STAT3 and STAT5 and estimated the relationship between STAT and cancer prognosis.
    Methods: One hundred patients who underwent gastrectomy due to gastric adenocarcinoma at Bundang CHA hospital between January 2000 and May 2005 were studied. Immunohistochemistry was carried out using antibodies against STAT3 and STAT5. The interpretation of the immunohistochemical staining was based on the proportion of stained cells in the field: positive, > 10% stained cells; and negative, < 10% stained cells.
    Results: The longest diameter of tumor was 4.67 cm in the positive group and 3.76 cm in the negative group, and these results were not statistically different (P-value = 0.112). Higher T (primary tumor) value (P-value = 0.05), more regional lymph node invasion (P-value = 0.008) and higher TNM staging (P-value = 0.069) were significantly related to STAT3 positivity, but Helicobacter pylori infection or atrophic gastritis were not related. A lower survival rate was observed in the STAT3-positive group (P-value = 0.001). The results of STAT5 were not statistically different with respect to TNM staging and survival (P-value = 0.958). We thus report that the immunohistochemical results of STAT3 revealed a significant association with TNM staging and survival.
    Conclusion: We anticipate that STAT3 may be used as a molecular staging biomarker predicting poor prognosis of gastric cancer.
    MeSH term(s) Adenocarcinoma/chemistry ; Adenocarcinoma/mortality ; Adenocarcinoma/pathology ; Adenocarcinoma/surgery ; Adolescent ; Adult ; Aged ; Biomarkers, Tumor/analysis ; Female ; Gastrectomy ; Humans ; Immunohistochemistry ; Kaplan-Meier Estimate ; Male ; Middle Aged ; Neoplasm Staging ; Predictive Value of Tests ; Retrospective Studies ; STAT3 Transcription Factor/analysis ; STAT5 Transcription Factor/analysis ; Stomach Neoplasms/chemistry ; Stomach Neoplasms/mortality ; Stomach Neoplasms/pathology ; Stomach Neoplasms/surgery ; Time Factors ; Treatment Outcome ; Young Adult
    Chemical Substances Biomarkers, Tumor ; STAT3 Transcription Factor ; STAT3 protein, human ; STAT5 Transcription Factor
    Language English
    Publishing date 2009-04
    Publishing country Australia
    Document type Journal Article
    ZDB-ID 632882-9
    ISSN 1440-1746 ; 0815-9319
    ISSN (online) 1440-1746
    ISSN 0815-9319
    DOI 10.1111/j.1440-1746.2008.05671.x
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 2180: Show issues Location:
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    Zs.MO 299: Show issues

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  6. Article ; Online: Leptin is an autocrine/paracrine regulator of wound healing.

    Murad, Ali / Nath, Anjali K / Cha, Sung-Tae / Demir, Erhan / Flores-Riveros, Jaime / Sierra-Honigmann, M Rocío

    FASEB journal : official publication of the Federation of American Societies for Experimental Biology

    2003  Volume 17, Issue 13, Page(s) 1895–1897

    Abstract: Leptin, a 16 kDa pleiotropic cytokine primarily expressed in adipose tissue, has been shown to cause multiple systemic biological actions. Recently, leptin has also been documented as an important component of the wound healing process and its receptor ... ...

    Abstract Leptin, a 16 kDa pleiotropic cytokine primarily expressed in adipose tissue, has been shown to cause multiple systemic biological actions. Recently, leptin has also been documented as an important component of the wound healing process and its receptor appears to be expressed in wound tissue. We have previously demonstrated that leptin is a potent angiogenic factor exerting direct effects on endothelial cells and that transcription of its encoding gene is regulated by hypoxia. Here, we hypothesize that leptin expression is acutely up-regulated in the ischemic tissue of experimental wounds. Using a combination of in situ hybridization and quantitative RT-PCR experiments, we show that leptin expression is rapidly and steadily up-regulated in skin tissue from incisional and excisional wounds. By immunohistochemistry, we demonstrate increased and sustained leptin protein levels in basal keratinocytes, blood vessel walls, and fibroblasts. To determine whether leptin is required for normal healing, excisional wounds were treated with neutralizing anti-leptin antibodies. This treatment markedly hampered healing progression and prevented wound closure and contraction. Finally, a transient rise in circulating blood leptin levels was detected within the first 24 h after inflicting the injury; we present evidence suggesting that this elevation is due to increased leptin production at the ischemic wound site. We conclude that leptin is acutely up-regulated in the injured skin and propose that this local production of leptin serves a critical functional role as an autocrine/paracrine regulator of normal wound healing.
    MeSH term(s) Animals ; Antibodies/pharmacology ; Autocrine Communication ; Leptin/analysis ; Leptin/metabolism ; Leptin/physiology ; Mice ; Models, Biological ; Paracrine Communication ; RNA, Messenger/biosynthesis ; Skin/anatomy & histology ; Skin/chemistry ; Skin/metabolism ; Wound Healing
    Chemical Substances Antibodies ; Leptin ; RNA, Messenger
    Language English
    Publishing date 2003-10
    Publishing country United States
    Document type Journal Article
    ZDB-ID 639186-2
    ISSN 1530-6860 ; 0892-6638
    ISSN (online) 1530-6860
    ISSN 0892-6638
    DOI 10.1096/fj.03-0068fje
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    Zs.MO 296: Show issues

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  7. Article: The usefulness of angiotensin converting enzyme in the differential diagnosis of Crohn's disease and intestinal tuberculosis.

    Kwon, Chang-Il / Park, Pil Won / Kang, Haeyoun / Kim, Gwang Il / Cha, Sung Tae / Kim, Kyung Soo / Ko, Kwang Hyun / Hong, Sung Pyo / Hwang, Seong Gyu / Rim, Kyu Sung

    The Korean journal of internal medicine

    2007  Volume 22, Issue 1, Page(s) 1–7

    Abstract: Background: Since the pathologic findings of Crohn's disease (CD) and intestinal tuberculosis (IT) overlap to a large degree, the development of other biomarkers will be of great help for making the differential diagnosis of these 2 diseases. The aim of ...

    Abstract Background: Since the pathologic findings of Crohn's disease (CD) and intestinal tuberculosis (IT) overlap to a large degree, the development of other biomarkers will be of great help for making the differential diagnosis of these 2 diseases. The aim of the present study is to examine the clinical efficacy of using the tissue angiotensin converting enzyme (ACE) assay in making the differential diagnosis between CD and IT.
    Methods: Tissue specimens were obtained from 36 patients who were diagnosed with CD or IT by the colonoscopic biopsy, as well as by the clinical findings. The expression of tissue ACE was detected by immunohistochemical staining. The optimal cut-off value of the immunoreactive scoring (IRS) system we used to differentiate CD from IT was determined by analysis of the ROC curve and AUROC.
    Results: Granuloma was present in 15 of 19 patients with CD (78.9%) and in 15 of 17 patients with IT (88.2%). ACE was present in the cytoplasm of the epithelioid cells in the granulomas from 13 of 15 patients with CD and in 14 of 15 patients with IT. The IRS scores of ACE were greater in the patients with CD than that of the patients with IT (8.07 +/- 4.38 vs. 4.13 +/- 2.47, respectively, p = 0.006). In differentiating CD from IT, the AUROC curve for the IRS of ACE was 0.767 with a sensitivity of 66.7%, a specificity of 93.3% and the cut-off point was 7.5.
    Conclusions: The results of our study suggest that the assessment of the tissue ACE expression can be helpful for making the differential diagnosis between CD and IT.
    MeSH term(s) Adolescent ; Adult ; Aged ; Biomarkers ; Colonoscopy ; Crohn Disease/diagnosis ; Crohn Disease/enzymology ; Crohn Disease/pathology ; Diagnosis, Differential ; Female ; Granuloma/pathology ; Humans ; Immunoenzyme Techniques ; Male ; Middle Aged ; Peptidyl-Dipeptidase A/metabolism ; Retrospective Studies ; Tuberculosis, Gastrointestinal/diagnosis ; Tuberculosis, Gastrointestinal/enzymology ; Tuberculosis, Gastrointestinal/pathology
    Chemical Substances Biomarkers ; Peptidyl-Dipeptidase A (EC 3.4.15.1)
    Language English
    Publishing date 2007-04-10
    Publishing country Korea (South)
    Document type Journal Article
    ZDB-ID 639023-7
    ISSN 1226-3303
    ISSN 1226-3303
    DOI 10.3904/kjim.2007.22.1.1
    Database MEDical Literature Analysis and Retrieval System OnLINE

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