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  1. Article ; Online: Application of PCR-based diagnostic tools that target Enterocytozoon hepatopenaei for the molecular detection of a Vittaforma-like microsporidium that infects Penaeus vannamei from Costa Rica

    Peña-Navarro, Nelson / López-Carvallo, Antonio / Chacón Perez, Brandon / Cruz-Flores, Roberto

    Journal of Invertebrate Pathology. 20232023 Sept. 08, July 08, v. 200 p.107958-

    2023  

    Abstract: Several PCR methodologies are available for the detection of Enterocytozoon hepatopenaei (EHP) that target the SSU rRNA gene. However, these methodologies are reported as unsuitable for the detection of EHP due to specificity issues. Here, we report the ... ...

    Abstract Several PCR methodologies are available for the detection of Enterocytozoon hepatopenaei (EHP) that target the SSU rRNA gene. However, these methodologies are reported as unsuitable for the detection of EHP due to specificity issues. Here, we report the applicability of two commonly used SSU rRNA methodologies for the detection of additional microsporidia from the genus Vittaforma that is present in cultured Penaeus vannamei from Costa Rica. The molecular detection of DNA of the novel microsporidia can only be achieved using SSU rRNA targeting methodologies and does not cross-react with the highly specific spore wall protein gene PCR detection method.
    Keywords DNA ; Enterocytozoon ; Litopenaeus vannamei ; genes ; invertebrates ; polymerase chain reaction ; spores ; Costa Rica ; Vittaforma sp. ; Enterocytozoon hepatopenaei ; Microsporidia ; Shrimp ; Penaeus vannamei
    Language English
    Dates of publication 2023-0708
    Publishing place Elsevier Inc.
    Document type Article ; Online
    ZDB-ID 390885-9
    ISSN 1096-0805 ; 0022-2011
    ISSN (online) 1096-0805
    ISSN 0022-2011
    DOI 10.1016/j.jip.2023.107958
    Database NAL-Catalogue (AGRICOLA)

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  2. Article ; Online: Application of PCR-based diagnostic tools that target Enterocytozoon hepatopenaei for the molecular detection of a Vittaforma-like microsporidium that infects Penaeus vannamei from Costa Rica.

    Peña-Navarro, Nelson / López-Carvallo, Antonio / Chacón Perez, Brandon / Cruz-Flores, Roberto

    Journal of invertebrate pathology

    2023  Volume 200, Page(s) 107958

    Abstract: Several PCR methodologies are available for the detection of Enterocytozoon hepatopenaei (EHP) that target the SSU rRNA gene. However, these methodologies are reported as unsuitable for the detection of EHP due to specificity issues. Here, we report the ... ...

    Abstract Several PCR methodologies are available for the detection of Enterocytozoon hepatopenaei (EHP) that target the SSU rRNA gene. However, these methodologies are reported as unsuitable for the detection of EHP due to specificity issues. Here, we report the applicability of two commonly used SSU rRNA methodologies for the detection of additional microsporidia from the genus Vittaforma that is present in cultured Penaeus vannamei from Costa Rica. The molecular detection of DNA of the novel microsporidia can only be achieved using SSU rRNA targeting methodologies and does not cross-react with the highly specific spore wall protein gene PCR detection method.
    MeSH term(s) Animals ; Microsporidia, Unclassified/genetics ; Penaeidae/genetics ; Vittaforma/genetics ; Costa Rica ; Polymerase Chain Reaction/methods ; Enterocytozoon/genetics ; Microsporidia/genetics ; RNA, Ribosomal
    Chemical Substances RNA, Ribosomal
    Language English
    Publishing date 2023-07-08
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 390885-9
    ISSN 1096-0805 ; 0022-2011
    ISSN (online) 1096-0805
    ISSN 0022-2011
    DOI 10.1016/j.jip.2023.107958
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.B 958: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

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