LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 5 of total 5

Search options

  1. Article ; Online: Meta-topolin induced in vitro regeneration in Crinum brachynema (Amaryllidaceae): a critically endangered and endemic medicinal plant of India

    Harmeet / Chahal, Swati / Lekhak, Manoj M. / Jha, Priyanka / Ochatt, Sergio / Kumar, Vijay

    Plant Cell Tiss Organ Cult. 2022 Dec., v. 151, no. 3 p.663-672

    2022  

    Abstract: Crinum brachynema (Amaryllidaceae) is an endemic and critically endangered bulbous medicinal plant of the Northern Western Ghats of India. This species contains an important and promising alkaloid called galanthamine (GAL), an anti-Alzheimer drug. This ... ...

    Abstract Crinum brachynema (Amaryllidaceae) is an endemic and critically endangered bulbous medicinal plant of the Northern Western Ghats of India. This species contains an important and promising alkaloid called galanthamine (GAL), an anti-Alzheimer drug. This investigation aimed to establish a reproducible protocol for in vitro regeneration of C. brachynema using twin scales as initial explants for the first time. Thus, cultures were established on Murashige and Skoog (MS) medium with cytokinins (6-benzyladenine (BA), and meta-Topolin (mT). The establishment and growth of shoots from the explants was achieved on MS medium supplemented with BA alone and in combination with 1-naphthaleneacetic acid (NAA). In vitro shoots were cultured on MS medium containing 0.5–10 µM of mT, of which 5.0 µM mT gave the highest shoot regeneration frequency (11.10). In general, mT proved superior as cultures produced a maximum number of regenerants compared to the control. Rooted plants with bulblets were successfully acclimatized in the greenhouse where they were phenotypically similar to the mother plants. Antioxidant activity was estimated using oxygen radical scavenging capacity (ORAC) model. Cultures on mT exhibited a significant increase in antioxidant capacity compared to the control. These findings highlight the beneficial effect and validate the rising importance of mT for in vitro regeneration studies. This study will serve as a potential protocol to conserve and restore the medicinally important C. brachynema, while the regenerated C. brachynema may be subsequently manipulated to further increase the accumulation of antioxidant potential.
    Keywords Brachynema ; Crinum ; alkaloids ; antioxidant activity ; benzyladenine ; cytokinins ; drugs ; greenhouses ; medicinal plants ; models ; naphthaleneacetic acid ; reactive oxygen species ; India
    Language English
    Dates of publication 2022-12
    Size p. 663-672.
    Publishing place Springer Netherlands
    Document type Article ; Online
    ZDB-ID 406394-6
    ISSN 1573-5044 ; 0167-6857
    ISSN (online) 1573-5044
    ISSN 0167-6857
    DOI 10.1007/s11240-022-02380-8
    Database NAL-Catalogue (AGRICOLA)

    More links

    Kategorien

    In stock of ZB MED Bonn / Germany

    Z 3642: Show issues

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  2. Article: Harnessing plant biotechnology-based strategies for in vitro galanthamine (GAL) biosynthesis: a potent drug against Alzheimer’s disease

    Kaur, Harmeet / Chahal, Swati / Jha, Priyanka / Lekhak, Manoj M. / Shekhawat, Mahipal S. / Naidoo, Devashan / Arencibia, Ariel D. / Ochatt, Sergio J. / Kumar, Vijay

    Plant cell, tissue, and organ culture. 2022 May, v. 149, no. 1-2

    2022  

    Abstract: Medicinal plants are important for improving human health and represent an essential pool for the identification of novel pharmacological leads. Plant-derived biomolecules have historically proven their value as a source of therapeutic drugs and hold an ... ...

    Abstract Medicinal plants are important for improving human health and represent an essential pool for the identification of novel pharmacological leads. Plant-derived biomolecules have historically proven their value as a source of therapeutic drugs and hold an important potential for the identification and characterization of novel drug leads. Many different alkaloids possessing a broad range of pharmacological activities have been isolated from plants belonging to the Amaryllidaceae family. Galanthamine (GAL) is a selective, reversible and an Amaryllidaceae-derived acetylcholinesterase inhibitor used for the treatment of Alzheimer’s disease (AD) and other neurological diseases. Naturally, the bioavailability of Amaryllidaceae alkaloids including GAL is low. Due to the significantly increased demand of GAL by the pharmaceutical industries and the inadequate availability of natural resources, in vitro culture offers an alternative approach for its sustainable production. Thus, different biotechnological tools can optimize the in vitro GAL biosynthesis for treating AD, such as manipulation of plant growth regulators, photoperiod, elicitors, and bioreactors systems, besides being an environmentally sustainable approach, which protects the native biodiversity in a circular bioeconomy context. In the present review, we highlight the biosynthesis of GAL by plant in vitro systems including its mode of action. This article should also provide a starting point in the scaling-up of the biotechnological production of this valuable alkaloid.
    Keywords Amaryllidaceae ; alkaloids ; bioavailability ; biodiversity ; bioeconomics ; bioreactors ; biosynthesis ; cholinesterase inhibitors ; drugs ; human health ; mechanism of action ; organ culture ; plant growth ; therapeutics
    Language English
    Dates of publication 2022-05
    Size p. 81-103.
    Publishing place Springer Netherlands
    Document type Article
    Note Review
    ZDB-ID 406394-6
    ISSN 1573-5044 ; 0167-6857
    ISSN (online) 1573-5044
    ISSN 0167-6857
    DOI 10.1007/s11240-022-02229-0
    Database NAL-Catalogue (AGRICOLA)

    More links

    Kategorien

    In stock of ZB MED Bonn / Germany

    Z 3642: Show issues

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  3. Article: Meta-topolin-mediated regeneration and accumulation of phenolic acids in the critically endangered medicinal plant Crinum malabaricum (Amaryllidaceae): A potent source of galanthamine

    Chahal, Swati / Kaur, Harmeet / Lekhak, Manoj M. / Shekhawat, Mahipal S. / Goutam, Umesh / Singh, Sachin Kumar / Ochatt, Sergio J. / Kumar, Vijay

    SAAB South African journal of botany. 2022 Jan. 03,

    2022  

    Abstract: Crinum malabaricum (Family: Amaryllidaceae) is a critically endangered aquatic medicinal plant endemic to India. This species is a promising natural source of bioactive compounds including galanthamine (GAL), an anti-Alzheimer drug. In vitro regeneration ...

    Abstract Crinum malabaricum (Family: Amaryllidaceae) is a critically endangered aquatic medicinal plant endemic to India. This species is a promising natural source of bioactive compounds including galanthamine (GAL), an anti-Alzheimer drug. In vitro regeneration in the Amaryllidaceae is often challenging. This study assessed the use of meta-Topolin (mT) on in vitro regeneration of C. malabaricum. Shoot explants were cultured on Murashige and Skoog (MS) medium supplemented with 0.5, 2.5, 5.0, 7.5 and 10.0 µM mT for six weeks, whereby 7.5 µM mT resulted in the maximum multiplication of adventitious shoots, much higher than the control. The biochemical accumulation of eleven different phenolic acids was quantified by UHPLC-MS/MS analysis, and it appeared that mT-treated cultures exhibited the highest concentration of phenolic acids. In particular, increased concentrations of gallic acid, protocatechuic acid, syringic acid, p-hydroxybenzoic acid, salicylic acid and vanillic acid were detected compared to the control. mT (2.5 and 5.0 µM) produced the maximum amount of chlorogenic acid, ferulic acid, p-coumaric acid and sinapic acid. However, an increased content of caffeic acid was produced on PGR-free medium. These findings highlight the beneficial effect and validate the rising importance of mT for in vitro regeneration studies. This study will serve as a potential protocol to conserve and restore the medically important C. malabaricum.
    Keywords 4-hydroxybenzoic acid ; Crinum ; caffeic acid ; chlorogenic acid ; drugs ; ferulic acid ; gallic acid ; medicinal plants ; p-coumaric acid ; protocatechuic acid ; salicylic acid ; sinapic acid ; syringic acid ; vanillic acid ; India
    Language English
    Dates of publication 2022-0103
    Publishing place Elsevier B.V.
    Document type Article
    Note Pre-press version
    ZDB-ID 2126918-X
    ISSN 0254-6299
    ISSN 0254-6299
    DOI 10.1016/j.sajb.2022.01.016
    Database NAL-Catalogue (AGRICOLA)

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  4. Article: Nardostachys jatamansi (D.Don) DC.: An invaluable and constantly dwindling resource of the Himalayas

    Kaur, Harmeet / Lekhak, Manoj M / Chahal, Swati / Goutam, Umesh / Jha, Priyanka / Naidoo, Devashan / Ochatt, Sergio J / Kumar, Vijay

    SAAB South African journal of botany. 2020 Dec., v. 135

    2020  

    Abstract: The use of medicinal plant species for different therapeutic effects is well recognized around the globe. Nardostachys jatamansi (D.Don) DC. (Family: Caprifoliaceae Juss.), commonly known as Indian spikenard is a critically endangered medicinal plant ... ...

    Abstract The use of medicinal plant species for different therapeutic effects is well recognized around the globe. Nardostachys jatamansi (D.Don) DC. (Family: Caprifoliaceae Juss.), commonly known as Indian spikenard is a critically endangered medicinal plant which grows at high altitudes in the alpine and sub-alpine regions of the Himalayas. Its medicinal use is well-recognized in the Bhutanese, Chinese, Indian, Japanese, Nepalese and Tibetan medicine. Moreover, its medicinal properties are well established in traditional medicines including Ayurveda, Ben-Cao- Shi-Yi, Homer's Iliad, the Old Testament, as in conventional systems. The increasing national and international demand of N. jatamansi, mostly for the rhizomes (underground tissue), as well as illegal/unsustainable harvesting has brought this valuable species to the edge of extinction. Therefore, more research input including in vitro biotechnological approaches is required to ensure its sustainable utilization and long-term conservation. Further research is also needed to improve our current knowledge about its conservation status, clinical relevance, and bioactive components. This review comprehensively summarizes the currently available information on the ethnomedicinal uses, pharmacology, phytochemistry, trade value and potential role of modern plant biotechnology tools for the conservation of this high value plant.
    Keywords Ayurvedic medicine ; Caprifoliaceae ; Nardostachys ; Oriental traditional medicine ; Valeriana jatamansi ; altitude ; bioactive compounds ; biotechnology ; conservation status ; extinction ; harvesting ; information ; knowledge ; medicinal plants ; medicinal properties ; plant biochemistry ; research ; rhizomes ; therapeutics ; trade ; Himalayan region
    Language English
    Dates of publication 2020-12
    Size p. 252-267.
    Publishing place Elsevier B.V.
    Document type Article
    Note NAL-light
    ZDB-ID 2126918-X
    ISSN 0254-6299
    ISSN 0254-6299
    DOI 10.1016/j.sajb.2020.08.010
    Database NAL-Catalogue (AGRICOLA)

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  5. Article: Unraveling the medicinal potential and conservation of Indian Crinum (Amaryllidaceae) species

    Chahal, Swati / Lekhak, Manoj M / Kaur, Harmeet / Shekhawat, Mahipal S / Dey, Abhijit / Jha, Priyanka / Pandey, Devendra Kumar / Kumar, Vijay

    SAAB South African journal of botany. 2021 Jan., v. 136

    2021  

    Abstract: The commercialization of medicinal plants as recognized in their uses by nutraceutical, cosmeceutical and pharmaceutical industries is gaining more popularity and interest around the globe. The plant family Amaryllidaceae has a long history in the ... ...

    Abstract The commercialization of medicinal plants as recognized in their uses by nutraceutical, cosmeceutical and pharmaceutical industries is gaining more popularity and interest around the globe. The plant family Amaryllidaceae has a long history in the traditional medicinal system and used to treat diverse diseases. It is well known for its structurally-diverse alkaloids which exhibit a wide range of pharmacological activities. Crinum, an important bulbous geophyte is well known for its ornamental and therapeutic properties. In India, Crinum is represented by 15 different species which contain several alkaloids with biological properties. However, some of the medicinal uses of Indian Crinum species still require pharmacological analysis. In addition, more extensive research including different biotechnological approaches is needed in order to guarantee its sustainable utilization and long-term conservation. Further research is essential to improve our current knowledge about the bioactive components, toxicity, clinical relevance as well as its conservation status. The present review summarizes the research that has been done on the phytochemistry, pharmacology and in vitro studies of the Indian Crinum. Potential toxicity aspects as well as knowledge gaps and future perspectives are also discussed.
    Keywords Crinum ; alkaloids ; bioactive compounds ; commercialization ; conservation status ; cosmetics ; dietary supplements ; geophytes ; history ; in vitro studies ; knowledge ; medicinal plants ; medicinal properties ; pharmaceutical industry ; plant biochemistry ; research ; toxicity ; India
    Language English
    Dates of publication 2021-01
    Size p. 7-15.
    Publishing place Elsevier B.V.
    Document type Article
    Note NAL-light
    ZDB-ID 2126918-X
    ISSN 0254-6299
    ISSN 0254-6299
    DOI 10.1016/j.sajb.2020.04.029
    Database NAL-Catalogue (AGRICOLA)

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

To top