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  1. AU="Chahboune, Halima"
  2. AU="Louache, Fawzia"
  3. AU="Ghanim, Husam"
  4. AU="Cassetta, Alberto"
  5. AU="Guan, Xiao-Li"
  6. AU="de Neve-Enthoven, N G M"
  7. AU="Konjeti R. Sekhar"
  8. AU="Dufresne, Eric M"
  9. AU="Pérez-Monje, Dan A"
  10. AU="Gazzola, Stefania"
  11. AU="Gruner, William"
  12. AU="Didichenko, Svetlana A"
  13. AU="Milone F."
  14. AU="Bianski, Brandon"
  15. AU="Swan, Aili"
  16. AU="Cao, Mengli"
  17. AU="Rayya, W."
  18. AU="Bleher, Jana"
  19. AU="Fong, Fuk-Kei"
  20. AU="Bouvier, Nicolas"
  21. AU="Mims, Catherine"
  22. AU="Abbasi, Ardeshir"
  23. AU="Gailey, Samantha" AU="Gailey, Samantha"
  24. AU="Yano, Shuya"
  25. AU="Balaur, Eugeniu"
  26. AU="Ağaçfidan, Ali"
  27. AU="Perkins, James A."
  28. AU="Arkoun, Brahim"

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  1. Artikel ; Online: Triptolide reduces cyst formation in a neonatal to adult transition Pkd1 model of ADPKD.

    Leuenroth, Stephanie J / Bencivenga, Natasha / Chahboune, Halima / Hyder, Fahmeed / Crews, Craig M

    Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association

    2010  Band 25, Heft 7, Seite(n) 2187–2194

    Abstract: Background: Autosomal dominant polycystic kidney disease (ADPKD), a major cause of end-stage renal failure, results from genetic mutation of either polycystin-1 (Pkd1) or polycystin-2 (Pkd2). In order to develop novel therapies to treat the advancement ... ...

    Abstract Background: Autosomal dominant polycystic kidney disease (ADPKD), a major cause of end-stage renal failure, results from genetic mutation of either polycystin-1 (Pkd1) or polycystin-2 (Pkd2). In order to develop novel therapies to treat the advancement of disease progression, numerous rodent models of different genetic backgrounds are available to study cyst development.
    Methods: Here, a Pkd1-floxed inducible mouse model using the interferon responsive Mx1Cre-recombinase was utilized to test the effect of the small molecule triptolide. Relative to other Pkd1 inactivation models, cyst progression in this neonatal to adult transition model is attenuated. Following the characterization of inducible cyst formation in these mice, the development of kidney cysts from triptolide or vehicle-treated animals was analysed.
    Results: Although Pkd1 deletion on postnatal Days P10 and P12 resulted in numerous cysts by P35, daily injections with triptolide beginning on Day P16 significantly reduced the total number of cysts per kidney, with a pronounced effect on the number of microcysts and the overall cystic burden. Additionally, renal function as assessed by blood urea nitrogen levels was also improved in triptolide-treated mice at both the P22 and P35 time points. As the Pkd1(flox/flox);Mx1Cre model has not been previously used for drug development studies, the feasibility of a 6-month adult Pkd1 inactivation study was also tested. While kidney cyst formation was minimal and focal in nature, livers of these Pkd1-deficient mice were severely cystic, enlarged and pale.
    Conclusions: These results suggest that the Pkd1(flox/flox);Mx1Cre model of ADPKD is amenable to short-term kidney cyst formation drug studies; however, it may be problematic for long-term therapeutic research where widespread liver cysts and fibrosis could compromise drug metabolism.
    Mesh-Begriff(e) Aging/pathology ; Animals ; Antineoplastic Agents, Alkylating/pharmacology ; Antineoplastic Agents, Alkylating/therapeutic use ; Cell Proliferation/drug effects ; Cyclin-Dependent Kinase Inhibitor p21/metabolism ; Disease Models, Animal ; Disease Progression ; Diterpenes/pharmacology ; Diterpenes/therapeutic use ; Epoxy Compounds/pharmacology ; Epoxy Compounds/therapeutic use ; GTP-Binding Proteins/genetics ; GTP-Binding Proteins/metabolism ; Integrases/genetics ; Integrases/metabolism ; Kidney/drug effects ; Kidney/metabolism ; Kidney/pathology ; Mice ; Mice, Mutant Strains ; Mutation/genetics ; Myxovirus Resistance Proteins ; Phenanthrenes/pharmacology ; Phenanthrenes/therapeutic use ; Polycystic Kidney, Autosomal Dominant/genetics ; Polycystic Kidney, Autosomal Dominant/physiopathology ; Polycystic Kidney, Autosomal Dominant/prevention & control ; TRPP Cation Channels/genetics ; TRPP Cation Channels/metabolism ; Time Factors
    Chemische Substanzen Antineoplastic Agents, Alkylating ; Cyclin-Dependent Kinase Inhibitor p21 ; Diterpenes ; Epoxy Compounds ; Myxovirus Resistance Proteins ; Phenanthrenes ; TRPP Cation Channels ; polycystic kidney disease 1 protein ; triptolide (19ALD1S53J) ; Cre recombinase (EC 2.7.7.-) ; Integrases (EC 2.7.7.-) ; GTP-Binding Proteins (EC 3.6.1.-)
    Sprache Englisch
    Erscheinungsdatum 2010-02-04
    Erscheinungsland England
    Dokumenttyp Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 90594-x
    ISSN 1460-2385 ; 0931-0509
    ISSN (online) 1460-2385
    ISSN 0931-0509
    DOI 10.1093/ndt/gfp777
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  2. Artikel ; Online: Induced clustered nanoconfinement of superparamagnetic iron oxide in biodegradable nanoparticles enhances transverse relaxivity for targeted theranostics.

    Ragheb, Ragy R T / Kim, Dongin / Bandyopadhyay, Arunima / Chahboune, Halima / Bulutoglu, Beyza / Ezaldein, Harib / Criscione, Jason M / Fahmy, Tarek M

    Magnetic resonance in medicine

    2013  Band 70, Heft 6, Seite(n) 1748–1760

    Abstract: Purpose: Combined therapeutic and diagnostic agents, "theranostics" are emerging valuable tools for noninvasive imaging and drug delivery. Here, we report on a solid biodegradable multifunctional nanoparticle that combines both features.: Methods: ... ...

    Abstract Purpose: Combined therapeutic and diagnostic agents, "theranostics" are emerging valuable tools for noninvasive imaging and drug delivery. Here, we report on a solid biodegradable multifunctional nanoparticle that combines both features.
    Methods: Poly(lactide-co-glycolide) nanoparticles were engineered to confine superparamagnetic iron oxide contrast for magnetic resonance imaging while enabling controlled drug delivery and targeting to specific cells. To achieve this dual modality, fatty acids were used as anchors for surface ligands and for encapsulated iron oxide in the polymer matrix.
    Results: We demonstrate that fatty acid modified iron oxide prolonged retention of the contrast agent in the polymer matrix during degradative release of drug. Antibody-fatty acid surface modification facilitated cellular targeting and subsequent internalization in cells while inducing clustering of encapsulated fatty-acid modified superparamagnetic iron oxide during particle formulation. This induced clustered confinement led to an aggregation within the nanoparticle and, hence, higher transverse relaxivity, r2 , (294 mM(-1) s(-1) ) compared with nanoparticles without fatty-acid ligands (160 mM(-1) s(-1) ) and higher than commercially available superparamagnetic iron oxide nanoparticles (89 mM(-1) s(-1) ).
    Conclusion: Clustering of superparamagnetic iron oxide in poly(lactide-co-glycolide) did not affect the controlled release of encapsulated drugs such as methotrexate or clodronate and their subsequent pharmacological activity, thus highlighting the full theranostic capability of our system.
    Mesh-Begriff(e) Absorbable Implants ; Animals ; Cells, Cultured ; Dextrans/chemistry ; Dextrans/therapeutic use ; Diffusion ; Drug Compounding/methods ; Macrophages/chemistry ; Magnetic Resonance Imaging/methods ; Magnetite Nanoparticles/chemistry ; Magnetite Nanoparticles/therapeutic use ; Materials Testing ; Melanoma, Experimental/chemistry ; Melanoma, Experimental/diagnosis ; Melanoma, Experimental/therapy ; Mice ; Nanocapsules/chemistry ; Nanocapsules/therapeutic use
    Chemische Substanzen Dextrans ; Magnetite Nanoparticles ; Nanocapsules ; ferumoxtran-10
    Sprache Englisch
    Erscheinungsdatum 2013-02-07
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ZDB-ID 605774-3
    ISSN 1522-2594 ; 0740-3194
    ISSN (online) 1522-2594
    ISSN 0740-3194
    DOI 10.1002/mrm.24622
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  3. Artikel: Effects of chloramphenicol on brain energy metabolism using ³¹P spectroscopy: influences on sleep-wake states in rat

    Chahboune, Halima / Mahdjoub, Rachid / Desgoutte, Pierre / Rousset, Colette / Briguet, André / Cespuglio, Raymond

    Journal of neurochemistry. 2008 Aug., v. 106, no. 4

    2008  

    Abstract: Effects of chloramphenicol (antibiotic inhibiting complex-1 of respiratory chain) and thioamphenicol (TAP, a structural analog of CAP inactive on complex-1) were examined on cerebral energy metabolites and sleep-wake cycle architecture in rat. In the ... ...

    Abstract Effects of chloramphenicol (antibiotic inhibiting complex-1 of respiratory chain) and thioamphenicol (TAP, a structural analog of CAP inactive on complex-1) were examined on cerebral energy metabolites and sleep-wake cycle architecture in rat. In the first group, animals were chronically equipped with a cranial surface resonator and ³¹P spectroscopic measurements were performed using a 2 T magnetic resonance spectrometer (operating frequency 34.46 MHz). CAP administration (400 mg/kg, tail vein, light period) induced deficits in phosphocreatine (-30%, p < 0.01) and ATP (-40%, p < 0.01), whereas TAP (400 mg/kg) had no effect. In the second group, animals were chronically implanted with polygraphic electrodes for EEG and electromyogram recordings. CAP administered intraperitoneally at light-onset reduced rapid-eye movement (REM) sleep (-60% in the first 6 h of light period, p < 0.01), increased waking state (+65% in the first 6 h of light period, p < 0.01), and slightly affected slow-wave sleep (SWS). During waking state, θ and σ power bands of the EEG were, respectively, increased and decreased (p < 0.05). During SWS, delta power band was reinforced (p < 0.05), while θ, α, and σ bands were decreased (p < 0.05). No changes occurred during REM sleep. TAP had no effect on sleep-wake states and spectral components of the EEG. Overall, these data indicate that REM sleep occurrence is linked to an aerobic production of ATP.
    Schlagwörter adenosine triphosphate ; chloramphenicol
    Sprache Englisch
    Erscheinungsverlauf 2008-08
    Umfang p. 1552-1562.
    Verlag Blackwell Publishing Ltd
    Erscheinungsort Oxford, UK
    Dokumenttyp Artikel
    ZDB-ID 80158-6
    ISSN 1471-4159 ; 0022-3042 ; 1474-1644
    ISSN (online) 1471-4159
    ISSN 0022-3042 ; 1474-1644
    DOI 10.1111/j.1471-4159.2008.05499.x
    Datenquelle NAL Katalog (AGRICOLA)

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  4. Artikel ; Online: Effects of chloramphenicol on brain energy metabolism using 31P spectroscopy: influences on sleep-wake states in rat.

    Chahboune, Halima / Mahdjoub, Rachid / Desgoutte, Pierre / Rousset, Colette / Briguet, André / Cespuglio, Raymond

    Journal of neurochemistry

    2008  Band 106, Heft 4, Seite(n) 1552–1562

    Abstract: Effects of chloramphenicol (antibiotic inhibiting complex-1 of respiratory chain) and thioamphenicol (TAP, a structural analog of CAP inactive on complex-1) were examined on cerebral energy metabolites and sleep-wake cycle architecture in rat. In the ... ...

    Abstract Effects of chloramphenicol (antibiotic inhibiting complex-1 of respiratory chain) and thioamphenicol (TAP, a structural analog of CAP inactive on complex-1) were examined on cerebral energy metabolites and sleep-wake cycle architecture in rat. In the first group, animals were chronically equipped with a cranial surface resonator and (31)P spectroscopic measurements were performed using a 2 T magnetic resonance spectrometer (operating frequency 34.46 MHz). CAP administration (400 mg/kg, tail vein, light period) induced deficits in phosphocreatine (-30%, p < 0.01) and ATP (-40%, p < 0.01), whereas TAP (400 mg/kg) had no effect. In the second group, animals were chronically implanted with polygraphic electrodes for EEG and electromyogram recordings. CAP administered intraperitoneally at light-onset reduced rapid-eye movement (REM) sleep (-60% in the first 6 h of light period, p < 0.01), increased waking state (+65% in the first 6 h of light period, p < 0.01), and slightly affected slow-wave sleep (SWS). During waking state, theta and sigma power bands of the EEG were, respectively, increased and decreased (p < 0.05). During SWS, delta power band was reinforced (p < 0.05), while theta, alpha, and sigma bands were decreased (p < 0.05). No changes occurred during REM sleep. TAP had no effect on sleep-wake states and spectral components of the EEG. Overall, these data indicate that REM sleep occurrence is linked to an aerobic production of ATP.
    Mesh-Begriff(e) Adenosine Triphosphate/deficiency ; Adenosine Triphosphate/metabolism ; Animals ; Brain/drug effects ; Brain/metabolism ; Chloramphenicol/pharmacology ; Electron Transport Complex I/deficiency ; Electron Transport Complex I/metabolism ; Energy Metabolism/drug effects ; Energy Metabolism/physiology ; Magnetic Resonance Spectroscopy/methods ; Male ; Phosphocreatine/deficiency ; Phosphocreatine/metabolism ; Phosphorus Radioisotopes/metabolism ; Rats ; Rats, Wistar ; Sleep/drug effects ; Sleep/physiology ; Sleep Stages/drug effects ; Sleep Stages/physiology ; Wakefulness/drug effects ; Wakefulness/physiology
    Chemische Substanzen Phosphorus Radioisotopes ; Phosphocreatine (020IUV4N33) ; Chloramphenicol (66974FR9Q1) ; Adenosine Triphosphate (8L70Q75FXE) ; Electron Transport Complex I (EC 1.6.5.3)
    Sprache Englisch
    Erscheinungsdatum 2008-08
    Erscheinungsland England
    Dokumenttyp Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 80158-6
    ISSN 1471-4159 ; 0022-3042 ; 1474-1644
    ISSN (online) 1471-4159
    ISSN 0022-3042 ; 1474-1644
    DOI 10.1111/j.1471-4159.2008.05499.x
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  5. Artikel: Neurodevelopment of C57B/L6 mouse brain assessed by in vivo diffusion tensor imaging.

    Chahboune, Halima / Ment, Laura R / Stewart, William B / Ma, Xiaoxian / Rothman, Douglas L / Hyder, Fahmeed

    NMR in biomedicine

    2007  Band 20, Heft 3, Seite(n) 375–382

    Abstract: Heterogeneous spatiotemporal patterns of C57B/L6 murine brain maturation during the first 7 weeks after birth (i.e. P15 to P45) were assessed in vivo by diffusion tensor imaging (DTI) at 9.4 T. Maps of apparent diffusion coefficient (ADC) and fractional ... ...

    Abstract Heterogeneous spatiotemporal patterns of C57B/L6 murine brain maturation during the first 7 weeks after birth (i.e. P15 to P45) were assessed in vivo by diffusion tensor imaging (DTI) at 9.4 T. Maps of apparent diffusion coefficient (ADC) and fractional anisotropy (FA) were used to assess developmental changes. Because directionally encoded color (DEC) maps provide an efficient and straightforward way to visualize anisotropy direction, they were used to highlight the orientation-dominant anisotropic tissues. In the corpus callosum, the increases in FA (approximately 0.4 to approximately 0.6 from P15 to P45) were primarily dominant in the medial-lateral direction, whereas the ADC decreased slightly (approximately 0.8 x 10(-3) to approximately 0.5 x 10(-3) mm(2)/s from P15 to P45). Similar increases in FA (approximately 0.3 to approximately 0.4 from P15 to P45) and decreases in ADC (approximately 0.8 x 10(-3) to approximately 0.5 x 10(-3) mm(2)/s from P15 to P45) were found in the cingulate, but these anisotropic changes were dominant in the anterior-posterior direction. In the caudate putamen, there were significant FA increases (approximately 0.1 to approximately 0.2 from P15 to P45) dominant in the dorsal-ventral and anterior-posterior directions, whereas the ADC increased rapidly early in development (approximately 0.3 x 10(-3) to approximately 0.7 x 10(-3) mm(2)/s from P15 to P17). There were no significant changes in tissue anisotropy in the somatosensory regions (whisker, forelimb), but the ADC decreased slightly (approximately 0.7 x 10(-3) to approximately 0.5 x 10(-3) mm(2)/s from P15 to P45). Although the major differences in DEC values were mainly observed in white matter pathways, other cortical and subcortical regions showed some potential morphological changes that were consistent with classical histological findings. In summary, these results show that high-resolution DTI at high magnetic fields allows detection and quantification of brain structures throughout normal development in C57B/L6 mice in vivo.
    Mesh-Begriff(e) Animals ; Anisotropy ; Brain/growth & development ; Brain Mapping ; Caudate Nucleus/growth & development ; Corpus Callosum/growth & development ; Diffusion ; Magnetic Resonance Imaging/methods ; Male ; Mice ; Mice, Inbred C57BL
    Sprache Englisch
    Erscheinungsdatum 2007-05
    Erscheinungsland England
    Dokumenttyp Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 1000976-0
    ISSN 1099-1492 ; 0952-3480
    ISSN (online) 1099-1492
    ISSN 0952-3480
    DOI 10.1002/nbm.1130
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  6. Artikel ; Online: Hypoxic injury during neonatal development in murine brain: correlation between in vivo DTI findings and behavioral assessment.

    Chahboune, Halima / Ment, Laura R / Stewart, William B / Rothman, Douglas L / Vaccarino, Flora M / Hyder, Fahmeed / Schwartz, Michael L

    Cerebral cortex (New York, N.Y. : 1991)

    2009  Band 19, Heft 12, Seite(n) 2891–2901

    Abstract: Preterm birth results in significant neurodevelopmental disability. A neonatal rodent model of chronic sublethal hypoxia (CSH), which mimics effects of preterm birth, was used to characterize neurodevelopmental consequences of prolonged exposure to ... ...

    Abstract Preterm birth results in significant neurodevelopmental disability. A neonatal rodent model of chronic sublethal hypoxia (CSH), which mimics effects of preterm birth, was used to characterize neurodevelopmental consequences of prolonged exposure to hypoxia using tissue anisotropy measurements from diffusion tensor imaging. Corpus callosum, cingulum, and fimbria of the hippocampus revealed subtle, yet significant, hypoxia-induced modifications during maturation (P15-P51). Anisotropy differences between control and CSH mice were greatest at older ages (>P40) in these regions. Neither somatosensory cortex nor caudate putamen revealed significant differences between control and CSH mice at any age. We assessed control and CSH mice using tests of general activity and cognition for behavioral correlates of morphological changes. Open-field task revealed greater locomotor activity in CSH mice early in maturation (P16-P18), whereas by adolescence (P40-P45) differences between control and CSH mice were insignificant. These results may be associated with lack of cortical and subcortical anisotropy differences between control and CSH mice. Spatial-delayed alternation and free-swim tasks in adulthood revealed lasting impairments for CSH mice in spatial memory and behavioral laterality. These differences may correlate with anisotropy decreases in hippocampal and callosal connectivities of CSH mice. Thus, CSH mice revealed developmental and behavioral deficits that are similar to those observed in low birth weight preterm infants.
    Mesh-Begriff(e) Animals ; Animals, Newborn ; Behavior, Animal ; Brain/pathology ; Brain/physiopathology ; Diffusion Magnetic Resonance Imaging/methods ; Hypoxia, Brain/embryology ; Hypoxia, Brain/pathology ; Hypoxia, Brain/physiopathology ; Male ; Mice ; Mice, Inbred C57BL ; Statistics as Topic
    Sprache Englisch
    Erscheinungsdatum 2009-04-20
    Erscheinungsland United States
    Dokumenttyp Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 1077450-6
    ISSN 1460-2199 ; 1047-3211
    ISSN (online) 1460-2199
    ISSN 1047-3211
    DOI 10.1093/cercor/bhp068
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  7. Artikel ; Online: Consequences of intraventricular hemorrhage in a rabbit pup model.

    Chua, Caroline O / Chahboune, Halima / Braun, Alex / Dummula, Krishna / Chua, Charles Edrick / Yu, Jen / Ungvari, Zoltan / Sherbany, Ariel A / Hyder, Fahmeed / Ballabh, Praveen

    Stroke

    2009  Band 40, Heft 10, Seite(n) 3369–3377

    Abstract: Background and purpose: Intraventricular hemorrhage (IVH) is a common complication of prematurity that results in neurological sequelae, including cerebral palsy, posthemorrhagic hydrocephalus, and cognitive deficits. Despite this, there is no ... ...

    Abstract Background and purpose: Intraventricular hemorrhage (IVH) is a common complication of prematurity that results in neurological sequelae, including cerebral palsy, posthemorrhagic hydrocephalus, and cognitive deficits. Despite this, there is no standardized animal model exhibiting neurological consequences of IVH in prematurely delivered animals. We asked whether induction of moderate-to-severe IVH in premature rabbit pups would produce long-term sequelae of cerebral palsy, posthemorrhagic hydrocephalus, reduced myelination, and gliosis.
    Methods: The premature rabbit pups, delivered by cesarean section, were treated with intraperitoneal glycerol at 2 hours postnatal age to induce IVH. The development of IVH was diagnosed by head ultrasound at 24 hours of age. Neurobehavioral, histological, and ultrastructural evaluation and diffusion tensor imaging studies were performed at 2 weeks of age.
    Results: Although 25% of pups with IVH (IVH pups) developed motor impairment with hypertonia and 42% developed posthemorrhagic ventriculomegaly, pups without IVH (non-IVH) were unremarkable. Immunolabeling revealed reduced myelination in the white matter of IVH pups compared with saline- and glycerol-treated non-IVH controls. Reduced myelination was confirmed by Western blot analysis. There was evidence of gliosis in IVH pups. Ultrastructural studies in IVH pups showed that myelinated and unmyelinated fibers were relatively preserved except for focal axonal injury. Diffusion tensor imaging showed reduction in fractional anisotropy and white matter volume confirming white matter injury in IVH pups.
    Conclusion: The rabbit pups with IVH displayed posthemorrhagic ventriculomegaly, gliosis, reduced myelination, and motor deficits, like humans. The study highlights an instructive animal model of the neurological consequences of IVH, which can be used to evaluate strategies in the prevention and treatment of posthemorrhagic complications.
    Mesh-Begriff(e) Age Factors ; Aging/physiology ; Animals ; Animals, Newborn ; Brain/anatomy & histology ; Brain/pathology ; Brain/physiopathology ; Cerebral Hemorrhage/complications ; Cerebral Hemorrhage/pathology ; Cerebral Hemorrhage/physiopathology ; Diffuse Axonal Injury/etiology ; Diffuse Axonal Injury/pathology ; Diffuse Axonal Injury/physiopathology ; Diffusion Magnetic Resonance Imaging ; Disease Models, Animal ; Gliosis/etiology ; Gliosis/pathology ; Gliosis/physiopathology ; Glycerol/toxicity ; Hypertrophy/etiology ; Hypertrophy/pathology ; Hypertrophy/physiopathology ; Lateral Ventricles/blood supply ; Lateral Ventricles/pathology ; Lateral Ventricles/physiopathology ; Microscopy, Electron, Transmission ; Movement Disorders/pathology ; Movement Disorders/physiopathology ; Nerve Fibers, Myelinated/pathology ; Premature Birth/pathology ; Premature Birth/physiopathology ; Rabbits ; Ultrasonography
    Chemische Substanzen Glycerol (PDC6A3C0OX)
    Sprache Englisch
    Erscheinungsdatum 2009-08-06
    Erscheinungsland United States
    Dokumenttyp Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 80381-9
    ISSN 1524-4628 ; 0039-2499 ; 0749-7954
    ISSN (online) 1524-4628
    ISSN 0039-2499 ; 0749-7954
    DOI 10.1161/STROKEAHA.109.549212
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  8. Artikel ; Online: Determining the fate of seeded cells in venous tissue-engineered vascular grafts using serial MRI.

    Harrington, Jamie K / Chahboune, Halima / Criscione, Jason M / Li, Alice Y / Hibino, Narutoshi / Yi, Tai / Villalona, Gustavo A / Kobsa, Serge / Meijas, Dane / Duncan, Daniel R / Devine, Lesley / Papademetri, Xenophon / Shin'oka, Toshiharu / Fahmy, Tarek M / Breuer, Christopher K

    FASEB journal : official publication of the Federation of American Societies for Experimental Biology

    2011  Band 25, Heft 12, Seite(n) 4150–4161

    Abstract: A major limitation of tissue engineering research is the lack of noninvasive monitoring techniques for observations of dynamic changes in single tissue-engineered constructs. We use cellular magnetic resonance imaging (MRI) to track the fate of cells ... ...

    Abstract A major limitation of tissue engineering research is the lack of noninvasive monitoring techniques for observations of dynamic changes in single tissue-engineered constructs. We use cellular magnetic resonance imaging (MRI) to track the fate of cells seeded onto functional tissue-engineered vascular grafts (TEVGs) through serial imaging. After in vitro optimization, murine macrophages were labeled with ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles and seeded onto scaffolds that were surgically implanted as inferior vena cava interposition grafts in SCID/bg mice. Serial MRI showed the transverse relaxation times (T(2)) were significantly lower immediately following implantation of USPIO-labeled scaffolds (T(2) = 44 ± 6.8 vs. 71 ± 10.2 ms) but increased rapidly at 2 h to values identical to control implants seeded with unlabeled macrophages (T(2) = 63 ± 12 vs. 63 ± 14 ms). This strongly indicates the rapid loss of seeded cells from the scaffolds, a finding verified using Prussian blue staining for iron containing macrophages on explanted TEVGs. Our results support a novel paradigm where seeded cells are rapidly lost from implanted scaffolds instead of developing into cells of the neovessel, as traditionally thought. Our findings confirm and validate this paradigm shift while demonstrating the first successful application of noninvasive MRI for serial study of cellular-level processes in tissue engineering.
    Mesh-Begriff(e) Animals ; Blood Vessel Prosthesis ; Cell Line ; Cell Survival ; Macrophages/cytology ; Macrophages/metabolism ; Magnetic Resonance Imaging ; Magnetite Nanoparticles ; Mice ; Mice, SCID ; Tissue Engineering ; Tissue Scaffolds ; Vena Cava, Inferior/cytology ; Vena Cava, Inferior/surgery
    Chemische Substanzen Magnetite Nanoparticles
    Sprache Englisch
    Erscheinungsdatum 2011-08-16
    Erscheinungsland United States
    Dokumenttyp Evaluation Study ; Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 639186-2
    ISSN 1530-6860 ; 0892-6638
    ISSN (online) 1530-6860
    ISSN 0892-6638
    DOI 10.1096/fj.11-185140
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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