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  1. Article: The occurrence of clubroot in cruciferous crops correlates with the chemical and microbial characteristics of soils.

    Kang, Huajun / Lin, Zihan / Yuan, Xiaowei / Shi, Yanxia / Xie, Xuewen / Li, Lei / Fan, Tengfei / Li, Baoju / Chai, Ali

    Frontiers in microbiology

    2024  Volume 14, Page(s) 1293360

    Abstract: Clubroot disease, caused ... ...

    Abstract Clubroot disease, caused by
    Language English
    Publishing date 2024-01-08
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2587354-4
    ISSN 1664-302X
    ISSN 1664-302X
    DOI 10.3389/fmicb.2023.1293360
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Application of ultra-low-volume spray for the control of vegetable disease in greenhouse: Investigation of formulation performance and potential dermal exposure.

    Ma, Jiayi / Xiang, Sheng / Shi, Yanxia / Xie, Xuewen / Chai, Ali / Li, Lei / Li, Baoju / Fan, Tengfei

    Pest management science

    2024  

    Abstract: Background: The use of pesticides in greenhouse vegetable cultivation is necessary and significant. However, traditional pesticide application methods such as the use of backpack sprayers with water-diluted pesticides have certain drawbacks, e.g., ... ...

    Abstract Background: The use of pesticides in greenhouse vegetable cultivation is necessary and significant. However, traditional pesticide application methods such as the use of backpack sprayers with water-diluted pesticides have certain drawbacks, e.g., uneven distribution, high labor intensity, and safety risks.
    Results: In this work, fluazinam ultra-low-volume liquids (Flu-ULVs) were prepared using oily solvents as carriers. The effects of different oils on the physical properties of the preparations were investigated. The Flu-ULV can be sprayed directly using a hand-held ultra-low-volume (ULV) sprayer without dilution with water. Compared with commercial water-based suspension concentrates of fluazinam, the Flu-ULV samples showed better wetting of tomato leaves, better atomization, and more uniform droplet distribution. At a dosage of 300 mL/ha, the coverage rate of tomato leaves ranged from 32.47% to 79.3%, with a droplet deposition density of 556 to 2017 droplets/cm
    Conclusion: Ground ULV spraying of fluazinam was proved to be an effective and safe management option for the control of tomato gray mold in greenhouses. This study laid a foundation for the use of ultra-low volume spray to control vegetable diseases in greenhouse, especially those induced by high humidity environment. © 2024 Society of Chemical Industry.
    Language English
    Publishing date 2024-02-05
    Publishing country England
    Document type Journal Article
    ZDB-ID 2001705-4
    ISSN 1526-4998 ; 1526-498X
    ISSN (online) 1526-4998
    ISSN 1526-498X
    DOI 10.1002/ps.7983
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Bonn / Germany

    Z 2731: Show issues

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  3. Article: Deciphering Differences in Microbial Community Diversity between Clubroot-Diseased and Healthy Soils.

    Kang, Huajun / Chai, Ali / Lin, Zihan / Shi, Yanxia / Xie, Xuewen / Li, Lei / Fan, Tengfei / Xiang, Sheng / Xie, Jianming / Li, Baoju

    Microorganisms

    2024  Volume 12, Issue 2

    Abstract: Clubroot ( ...

    Abstract Clubroot (
    Language English
    Publishing date 2024-01-25
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2720891-6
    ISSN 2076-2607
    ISSN 2076-2607
    DOI 10.3390/microorganisms12020251
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Monitoring

    Zhou, Rongjia / Sun, Bingxue / Zhu, Guangxue / Xie, Xuewen / Chai, Ali / Li, Lei / Fan, Tengfei / Li, Baoju / Shi, Yanxia

    Phytopathology

    2024  Volume 114, Issue 2, Page(s) 359–367

    Abstract: Cucumber leaf spot (CLS), caused ... ...

    Abstract Cucumber leaf spot (CLS), caused by
    MeSH term(s) Fungicides, Industrial/pharmacology ; Plant Diseases ; China ; Cucumis sativus ; Benzimidazoles ; Niacinamide/analogs & derivatives ; Imines ; Ascomycota ; Strobilurins ; Carbamates ; Acetates ; Biphenyl Compounds
    Chemical Substances trifloxystrobin (F625Z36B2D) ; Fungicides, Industrial ; 2-chloro-N-(4-chlorobiphenyl-2-yl)nicotinamide (32MS8ZRD1V) ; carbendazim (H75J14AA89) ; Benzimidazoles ; Niacinamide (25X51I8RD4) ; Imines ; Strobilurins ; Carbamates ; Acetates ; Biphenyl Compounds
    Language English
    Publishing date 2024-02-19
    Publishing country United States
    Document type Journal Article
    ZDB-ID 208889-7
    ISSN 1943-7684 ; 0031-949X
    ISSN (online) 1943-7684
    ISSN 0031-949X
    DOI 10.1094/PHYTO-06-23-0186-R
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.B 698: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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  5. Article: Identification and Genetic Diversity Analysis of the Pathogen of Anthracnose of Pepper in Guizhou.

    Zhang, Aimin / Li, Lei / Xie, Xuewen / Chai, Ali / Shi, Yanxia / Xing, Dan / Yu, Zhiguo / Li, Baoju

    Plants (Basel, Switzerland)

    2024  Volume 13, Issue 5

    Abstract: Anthracnose of pepper is a significant disease caused ... ...

    Abstract Anthracnose of pepper is a significant disease caused by
    Language English
    Publishing date 2024-03-04
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2704341-1
    ISSN 2223-7747
    ISSN 2223-7747
    DOI 10.3390/plants13050728
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: Effect of temperature and humidity on dynamics and transmission of

    Chai, Ali / Yuan, Lifang / Li, Xin / Li, Lei / Shi, Yanxia / Xie, Xuewen / Li, Baoju

    Frontiers in plant science

    2023  Volume 14, Page(s) 1087496

    Abstract: Cucumber angular leaf spot (ALS) disease, caused ... ...

    Abstract Cucumber angular leaf spot (ALS) disease, caused by
    Language English
    Publishing date 2023-02-03
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2613694-6
    ISSN 1664-462X
    ISSN 1664-462X
    DOI 10.3389/fpls.2023.1087496
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Calcium carbonate‐modified plant sporopollen capsule as an eco‐friendly microvehicle for controlled release of pesticide

    Xiang, Sheng / Kang, Huajun / Chai, Ali / Shi, Yanxia / Xie, Xuewen / Li, Lei / Fan, Tengfei / Li, Baoju

    Pest Management Science. 2023 Apr., v. 79, no. 4 p.1604-1614

    2023  

    Abstract: BACKGROUND: In this work, natural club moss (Lycopodium clavatum, LC) spores with a porous surface morphology and highly uniform size distribution were engineered into controlled‐release microvehicles for pesticide delivery. As a proof of concept, a ... ...

    Abstract BACKGROUND: In this work, natural club moss (Lycopodium clavatum, LC) spores with a porous surface morphology and highly uniform size distribution were engineered into controlled‐release microvehicles for pesticide delivery. As a proof of concept, a widely used fungicide, fluazinam (FLU), was successfully loaded into LC spores and then modified with different amounts of CaCO₃ (CaC) to extend the efficacy duration of FLU. Significantly, as the control target of FLU, clubroot disease is a worldwide destructive disease of cruciferous crops, and its development is favored by acidic soils and can be suppressed at high Ca concentrations. RESULTS: Fabricated FLU@LC‐CaC microcapsules, FLU loading and CaCO₃ deposition were systematically characterized by field emission scanning electron microscopy, Fourier transform infrared spectroscopy, and thermogravimetric analysis. The as‐prepared FLU@LC‐CaC microcapsules showed sustained‐release behaviors and were potentially able to supplement the Ca concentration in acidic environments. This approach synergistically enhanced in vivo bioactivity for the on‐demand control of clubroot disease. An in vivo bioassay revealed that the control efficacy of FLU@LC‐CaC against clubroot disease in pak choi (Brassica chinensis) (66.4%) was 1.7‐fold higher than that of a commercial FLU suspension concentrate (38.2%) over the course of the cultivation period (35 days). CONCLUSIONS: This work provides new ideas not only for developing eco‐friendly and scalable microvehicles for pesticide delivery based on natural sporopollen, but also for unconventional research perspectives in on‐demand pest management based on their occurrence characteristics. © 2022 Society of Chemical Industry.
    Keywords Brassica rapa subsp. chinensis ; Fourier transform infrared spectroscopy ; Lycopodium ; bioactive properties ; bioassays ; calcium ; clubroot ; electron microscopy ; fungicides ; mosses and liverworts ; thermogravimetry
    Language English
    Dates of publication 2023-04
    Size p. 1604-1614.
    Publishing place John Wiley & Sons, Ltd.
    Document type Article ; Online
    Note JOURNAL ARTICLE
    ZDB-ID 2001705-4
    ISSN 1526-4998 ; 1526-498X
    ISSN (online) 1526-4998
    ISSN 1526-498X
    DOI 10.1002/ps.7333
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 2731: Show issues

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  8. Article: Development of a Real-Time Quantitative PCR Assay for the Specific Detection of Bacillus velezensis and Its Application in the Study of Colonization Ability

    Xu, Shuai / Xie, Xuewen / Shi, Yanxia / Chai, Ali / Li, Baoju / Li, Lei

    Microorganisms. 2022 June 14, v. 10, no. 6

    2022  

    Abstract: Bacillus velezensis is a widely used biocontrol agent closely related to B. amyloliquefaciens, and the two species cannot be distinguished by universal primers that are currently available. The study aimed to establish a rapid, specific detection ... ...

    Abstract Bacillus velezensis is a widely used biocontrol agent closely related to B. amyloliquefaciens, and the two species cannot be distinguished by universal primers that are currently available. The study aimed to establish a rapid, specific detection approach for B. velezensis. Many unique gene sequences of B. velezensis were selected through whole genome sequence alignment of B. velezensis strains and were used to design a series of forward and reverse primers, which were then screened by PCR and qPCR using different Bacillus samples as templates. The colonization ability of B. velezensis ZF2 in different soils and different soil environmental conditions was measured by qPCR and a 10-fold dilution plating assay. A specific primer pair targeting the sequence of the D3N19_RS13500 gene of B. velezensis ZF2 was screened and could successfully distinguish B. velezensis from B. amyloliquefaciens. A rapid specific real-time qPCR detection system for B. velezensis was established. B. velezensis ZF2 had a very strong colonization ability in desert soil, and the optimal soil pH was 7–8. Moreover, the colonization ability of strain ZF2 was significantly enhanced when organic matter from different nitrogen sources was added to the substrate. This study will provide assistance for rapid specificity detection and biocontrol application of B. velezensis strains.
    Keywords Bacillus velezensis ; biological control ; biological control agents ; colonizing ability ; desert soils ; genes ; nitrogen ; nucleotide sequences ; oligodeoxyribonucleotides ; organic matter ; quantitative polymerase chain reaction ; sequence alignment ; soil pH
    Language English
    Dates of publication 2022-0614
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article
    ZDB-ID 2720891-6
    ISSN 2076-2607
    ISSN 2076-2607
    DOI 10.3390/microorganisms10061216
    Database NAL-Catalogue (AGRICOLA)

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  9. Article: Double Mutations in Succinate Dehydrogenase Are Involved in SDHI Resistance in Corynespora cassiicola

    Sun, Bingxue / Zhu, Guangxue / Xie, Xuewen / Chai, Ali / Li, Lei / Shi, Yanxia / Li, Baoju

    Microorganisms. 2022 Jan. 09, v. 10, no. 1

    2022  

    Abstract: With the further application of succinate dehydrogenase inhibitors (SDHI), the resistance caused by double mutations in target gene is gradually becoming a serious problem, leading to a decrease of control efficacy. It is important to assess the ... ...

    Abstract With the further application of succinate dehydrogenase inhibitors (SDHI), the resistance caused by double mutations in target gene is gradually becoming a serious problem, leading to a decrease of control efficacy. It is important to assess the sensitivity and fitness of double mutations to SDHI in Corynespora cassiicola and analysis the evolution of double mutations. We confirmed, by site-directed mutagenesis, that all double mutations (B-I280V+D-D95E/D-G109V/D-H105R, B-H278R+D-D95E/D-G109V, B-H278Y+D-D95E/D-G109V) conferred resistance to all SDHI and exhibited the increased resistance to at least one fungicide than single point mutation. Analyses of fitness showed that all double mutations had lower fitness than the wild type; most of double mutations suffered more fitness penalties than the corresponding single mutants. We also further found that double mutations (B-I280V+D-D95E/D-G109V/D-H105R) containing low SDHI-resistant single point mutation (B-I280V) exhibited higher resistance to SDHI and low fitness penalty than double mutations (B-H278Y+D-D95E/D-G109V) containing high SDHI-resistant single mutations (B-H278Y). Therefore, we may infer that a single mutation conferring low resistance is more likely to evolve into a double mutation conferring higher resistance under the selective pressure of SDHI. Taken together, our results provide some important reference for resistance management.
    Keywords Corynespora cassiicola ; evolution ; fungicides ; genes ; point mutation ; resistance management ; site-directed mutagenesis ; succinate dehydrogenase (quinone)
    Language English
    Dates of publication 2022-0109
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article
    ZDB-ID 2720891-6
    ISSN 2076-2607
    ISSN 2076-2607
    DOI 10.3390/microorganisms10010132
    Database NAL-Catalogue (AGRICOLA)

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  10. Article: Emergence of bacterial soft rot in calla lily caused by Pectobacterium aroidearum in China

    Li, Lei / Yuan, Lifang / Zhao, Yurong / Shi, Yanxia / Chai, Ali / Xie, Xuewen / Li, Baoju

    Crop protection. 2022 Feb., v. 152

    2022  

    Abstract: Bacterial soft rot is a destructive disease affecting calla lily production, and it is mainly caused by Pectobacterium carotovorum (Pc). In 2019, this destructive disease was observed in the Shandong and Jiangsu Provinces of China and resulted in serious ...

    Abstract Bacterial soft rot is a destructive disease affecting calla lily production, and it is mainly caused by Pectobacterium carotovorum (Pc). In 2019, this destructive disease was observed in the Shandong and Jiangsu Provinces of China and resulted in serious economic losses for commercial greenhouse producers of ornamental plants. The disease symptoms on leaves and stems were yellow and dark brown patches, and then water-soaked lesions that turned to wet rot occurred on the stems. In total, we obtained twenty-eight calla lily soft rot samples collected from Shandong and Jiangsu. Fifteen colonies on LB medium were milky white, nearly round, with a smooth surface and neat edges. The soft rot symptoms in calla lily caused by artificial inoculation were the same as the natural symptoms in the field. Based on the phenotypic properties determined by morphological, biochemical and physiological analyses, 16S ribosomal DNA gene sequence analysis, and multilocus sequence analysis, the pathogen was identified as P. aroidearum. In addition, all P. aroidearum strains exhibited high virulence and a wide host range on monocotyledonous and dicotyledonous plants. This study is the first official report of P. aroidearum causing calla lily soft rot in China, and the results contribute to a better understanding of the disease.
    Keywords Pectobacterium carotovorum ; Zantedeschia aethiopica ; bacterial soft rot ; greenhouses ; host range ; multilocus sequence typing ; nucleotide sequences ; pathogens ; phenotype ; plant protection ; ribosomal DNA ; virulence ; China
    Language English
    Dates of publication 2022-02
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 786839-x
    ISSN 1873-6904 ; 0261-2194
    ISSN (online) 1873-6904
    ISSN 0261-2194
    DOI 10.1016/j.cropro.2021.105854
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