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  1. Article: Urine and Saliva: Relevant Specimens for Malaria Diagnosis?

    Chai, Hwa Chia / Chua, Kek Heng

    Diagnostics (Basel, Switzerland)

    2022  Volume 12, Issue 12

    Abstract: Blood remains the specimen of preference for malaria diagnosis, whether it is for microscopic, nucleic acid-based or biomarker detection ... ...

    Abstract Blood remains the specimen of preference for malaria diagnosis, whether it is for microscopic, nucleic acid-based or biomarker detection of
    Language English
    Publishing date 2022-11-29
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2662336-5
    ISSN 2075-4418
    ISSN 2075-4418
    DOI 10.3390/diagnostics12122989
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  2. Article: Exploring MiR-484 Regulation by

    Niu, Jiaojiao / Chen, Yeng / Chai, Hwa Chia / Sasidharan, Sreenivasan

    Biomedicines

    2024  Volume 12, Issue 4

    Abstract: Background: MiR-484, implicated in various carcinomas, holds promise as a prognostic marker, yet its relevance to cervical cancer (CC) remains unclear. Our prior study demonstrated the : Methods: MiR-484 levels were analyzed across cancers, including ...

    Abstract Background: MiR-484, implicated in various carcinomas, holds promise as a prognostic marker, yet its relevance to cervical cancer (CC) remains unclear. Our prior study demonstrated the
    Methods: MiR-484 levels were analyzed across cancers, including CC, from The Cancer Genome Atlas. The limma R package identified differentially expressed genes (DEGs) between high- and low-miR-484 CC cohorts. We assessed biological functions, tumor microenvironment (TME), immunotherapy, stemness, hypoxia, RNA methylation, and chemosensitivity differences. Prognostic genes relevant to miR-484 were identified through Cox regression and Kaplan-Meier analyses, and a prognostic model was captured via multivariate Cox regression. Single-cell RNA sequencing determined cell populations related to prognostic genes. qRT-PCR validated key genes, and the miR-484 effect on CC proliferation was assessed via an MTT assay.
    Results: MiR-484 was upregulated in most tumors, including CC, with DEGs enriched in skin development, PI3K signaling, and immune processes. High miR-484 expression correlated with specific immune cell infiltration, hypoxia, and drug sensitivity. Prognostic genes identified were predominantly epidermal and stratified patients with CC into risk groups, with the low-risk group showing enhanced survival and immunotherapeutic responses. qRT-PCR confirmed FGFR3 upregulation in CC cells, and an miR-484 mimic reversed the
    Conclusion: MiR-484 plays a crucial role in the CC progression and prognosis, suggesting its potential as a biomarker for targeted therapy.
    Language English
    Publishing date 2024-04-19
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2720867-9
    ISSN 2227-9059
    ISSN 2227-9059
    DOI 10.3390/biomedicines12040909
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  3. Article: The Potential Use of Volatile Biomarkers for Malaria Diagnosis.

    Chai, Hwa Chia / Chua, Kek Heng

    Diagnostics (Basel, Switzerland)

    2021  Volume 11, Issue 12

    Abstract: Pathogens may change the odor and odor-related biting behavior of the vector and host to enhance pathogen transmission. In recent years, volatile biomarker investigations have emerged to identify odors that are differentially and specifically released by ...

    Abstract Pathogens may change the odor and odor-related biting behavior of the vector and host to enhance pathogen transmission. In recent years, volatile biomarker investigations have emerged to identify odors that are differentially and specifically released by pathogens and plants, or the pathogen-infected or even cancer patients. Several studies have reported odors or volatile biomarkers specifically detected from the breath and skin of malaria-infected individuals. This review will discuss the potential use of these odors or volatile biomarkers for the diagnosis of malaria. This approach not only allows for the non-invasive mean of sample collection but also opens up the opportunity to develop a biosensor for malaria diagnosis in low-resource settings.
    Language English
    Publishing date 2021-11-30
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2662336-5
    ISSN 2075-4418
    ISSN 2075-4418
    DOI 10.3390/diagnostics11122244
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  4. Article ; Online: The first association study of Protein Tyrosine Phosphatase, Non-Receptor Type 2 (PTPN2) gene polymorphisms in Malaysian patients with Crohn's disease.

    Goh, Xiang Ting / Fong, Suh Kuan / Chai, Hwa Chia / Kee, Boon Pin / Chua, Kek Heng

    Gene

    2022  Volume 836, Page(s) 146661

    Abstract: Crohn's disease (CD) is one of the sub-entities of Inflammatory Bowel Disease which causes chronic inflammation in the gastrointestinal tract. The development of CD has shown to have a strong genetic association. Therefore, the present study aimed to ... ...

    Abstract Crohn's disease (CD) is one of the sub-entities of Inflammatory Bowel Disease which causes chronic inflammation in the gastrointestinal tract. The development of CD has shown to have a strong genetic association. Therefore, the present study aimed to investigate the association between genetic polymorphisms in a susceptible locus of CD, the protein tyrosine phosphatase, non-receptor type 2 (PTPN2) gene and the development of CD in Malaysian patients. A total of 137 CD patients and 274 matched healthy controls were recruited in the present study. Genomic DNA was extracted from the venous blood of participants and five targeted single nucleotide polymorphisms (SNPs) in the PTPN2 gene were genotyped using polymerase chain reaction. Associations between the SNPs and CD were determined using Fisher's exact test and odds ratio. Findings showed that all five selected SNPs were not significantly associated with the development of CD in Malaysian patients, which was in contrast to studies among the European populations. Malaysian Chinese with rs487273 heterozygous G/T genotype was found to have a lower occurrence of CD (P-value = 0.0253; OR = 0.4396). Patients with rs2542152 homozygous T genotype were associated with stricturing behaviour (P-value = 0.0302, OR = 2.9944). The rs16939895 A/G genotype was associated with inflammation at the ileum site (P-value = 0.0387, OR = 2.2105)while homozygous G genotype was associated with colonic CD (P-value = 0.0164, OR = 2.3917). Functional studies of these SNPs are needed to evaluate their potential use as a biomarker for disease phenotypes among Asian patients.
    MeSH term(s) Crohn Disease/genetics ; Genetic Predisposition to Disease ; Genotype ; Humans ; Inflammation ; Malaysia ; Polymorphism, Single Nucleotide ; Protein Tyrosine Phosphatase, Non-Receptor Type 2/genetics ; Protein Tyrosine Phosphatase, Non-Receptor Type 2/metabolism
    Chemical Substances PTPN2 protein, human (EC 3.1.3.48) ; Protein Tyrosine Phosphatase, Non-Receptor Type 2 (EC 3.1.3.48)
    Language English
    Publishing date 2022-06-06
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 391792-7
    ISSN 1879-0038 ; 0378-1119
    ISSN (online) 1879-0038
    ISSN 0378-1119
    DOI 10.1016/j.gene.2022.146661
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1357: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
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  5. Article: The first association study of Protein Tyrosine Phosphatase, Non-Receptor Type 2 (PTPN2) gene polymorphisms in Malaysian patients with Crohn’s disease

    Goh, Xiang Ting / Fong, Suh Kuan / Chai, Hwa Chia / Kee, Boon Pin / Chua, Kek Heng

    Gene. 2022 Aug. 20, v. 836

    2022  

    Abstract: Crohn’s disease (CD) is one of the sub-entities of Inflammatory Bowel Disease which causes chronic inflammation in the gastrointestinal tract. The development of CD has shown to have a strong genetic association. Therefore, the present study aimed to ... ...

    Abstract Crohn’s disease (CD) is one of the sub-entities of Inflammatory Bowel Disease which causes chronic inflammation in the gastrointestinal tract. The development of CD has shown to have a strong genetic association. Therefore, the present study aimed to investigate the association between genetic polymorphisms in a susceptible locus of CD, the protein tyrosine phosphatase, non-receptor type 2 (PTPN2) gene and the development of CD in Malaysian patients. A total of 137 CD patients and 274 matched healthy controls were recruited in the present study. Genomic DNA was extracted from the venous blood of participants and five targeted single nucleotide polymorphisms (SNPs) in the PTPN2 gene were genotyped using polymerase chain reaction. Associations between the SNPs and CD were determined using Fisher’s exact test and odds ratio. Findings showed that all five selected SNPs were not significantly associated with the development of CD in Malaysian patients, which was in contrast to studies among the European populations. Malaysian Chinese with rs487273 heterozygous G/T genotype was found to have a lower occurrence of CD (P-value = 0.0253; OR = 0.4396). Patients with rs2542152 homozygous T genotype were associated with stricturing behaviour (P-value = 0.0302, OR = 2.9944). The rs16939895 A/G genotype was associated with inflammation at the ileum site (P-value = 0.0387, OR = 2.2105)while homozygous G genotype was associated with colonic CD (P-value = 0.0164, OR = 2.3917). Functional studies of these SNPs are needed to evaluate their potential use as a biomarker for disease phenotypes among Asian patients.
    Keywords DNA ; biomarkers ; blood ; digestive tract ; genes ; genotyping ; heterozygosity ; homozygosity ; ileum ; inflammation ; loci ; odds ratio ; polymerase chain reaction ; protein-tyrosine-phosphatase
    Language English
    Dates of publication 2022-0820
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 391792-7
    ISSN 1879-0038 ; 0378-1119
    ISSN (online) 1879-0038
    ISSN 0378-1119
    DOI 10.1016/j.gene.2022.146661
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    In stock of ZB MED Bonn / Germany

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  6. Article: Proteomic profiling of clinical and environmental strains of Pseudomonas aeruginosa

    Liew, Siew Mun / Puthucheary, Savithiri D / Rajasekaram, Ganeswrei / Chai, Hwa Chia / Chua, Kek Heng

    Molecular biology reports. 2021 Mar., v. 48, no. 3

    2021  

    Abstract: Pseudomonas aeruginosa is a ubiquitous bacterium, which is able to change its physiological characteristics in response to different habitats. Environmental strains are presumably less pathogenic than clinical strains and whether or not the clinical ... ...

    Abstract Pseudomonas aeruginosa is a ubiquitous bacterium, which is able to change its physiological characteristics in response to different habitats. Environmental strains are presumably less pathogenic than clinical strains and whether or not the clinical strains originate from the environment or through inter-host transmission remains poorly understood. To minimize the risk of infection, a better understanding of proteomic profiling of P. aeruginosa is necessary for elucidating the correlation between environmental and clinical strains. Based on antimicrobial susceptibility and patterns of virulence, we selected 12 clinical and environmental strains: (i) environmental, (ii) multidrug resistant (MDR) clinical and (iii) susceptible clinical strains. Whole-cell protein was extracted from each strain and subjected to two-dimensional differential gel electrophoresis (2-D DIGE) and liquid chromatography tandem mass spectrometry quadrupole time-of-flight (LC-MS QTOF). All 12 strains were clustered into 3 distinct groups based on their variance in protein expression. A total of 526 matched spots were detected and four differentially expressed protein spots (p < 0.05) were identified and all differential spots were downregulated in MDR strain J3. Upregulation of chitin binding and BON domain proteins was present in the environmental and some MDR strains, whereas the clinical strains exhibited distinct proteomic profiles with increased expression of serine protein kinase and arginine/ornithine transport ATP-binding proteins. Significant difference in expression was observed between susceptible clinical and MDR strains, as well as susceptible clinical and environmental strains. Transition from an environmental saprophyte to a clinical strain could alter its physiological characteristics to further increase its adaptation.
    Keywords Pseudomonas aeruginosa ; antibiotic resistance ; arginine ; bacteria ; chitin ; gel electrophoresis ; gene expression regulation ; liquid chromatography ; molecular biology ; multiple drug resistance ; non-specific serine/threonine protein kinase ; ornithine ; protein synthesis ; proteomics ; risk ; saprophytes ; tandem mass spectrometry ; variance ; virulence
    Language English
    Dates of publication 2021-03
    Size p. 2325-2333.
    Publishing place Springer Netherlands
    Document type Article
    Note NAL-AP-2-clean
    ZDB-ID 186544-4
    ISSN 1573-4978 ; 0301-4851
    ISSN (online) 1573-4978
    ISSN 0301-4851
    DOI 10.1007/s11033-021-06262-8
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  7. Article ; Online: Proteomic profiling of clinical and environmental strains of Pseudomonas aeruginosa.

    Liew, Siew Mun / Puthucheary, Savithiri D / Rajasekaram, Ganeswrei / Chai, Hwa Chia / Chua, Kek Heng

    Molecular biology reports

    2021  Volume 48, Issue 3, Page(s) 2325–2333

    Abstract: Pseudomonas aeruginosa is a ubiquitous bacterium, which is able to change its physiological characteristics in response to different habitats. Environmental strains are presumably less pathogenic than clinical strains and whether or not the clinical ... ...

    Abstract Pseudomonas aeruginosa is a ubiquitous bacterium, which is able to change its physiological characteristics in response to different habitats. Environmental strains are presumably less pathogenic than clinical strains and whether or not the clinical strains originate from the environment or through inter-host transmission remains poorly understood. To minimize the risk of infection, a better understanding of proteomic profiling of P. aeruginosa is necessary for elucidating the correlation between environmental and clinical strains. Based on antimicrobial susceptibility and patterns of virulence, we selected 12 clinical and environmental strains: (i) environmental, (ii) multidrug resistant (MDR) clinical and (iii) susceptible clinical strains. Whole-cell protein was extracted from each strain and subjected to two-dimensional differential gel electrophoresis (2-D DIGE) and liquid chromatography tandem mass spectrometry quadrupole time-of-flight (LC-MS QTOF). All 12 strains were clustered into 3 distinct groups based on their variance in protein expression. A total of 526 matched spots were detected and four differentially expressed protein spots (p < 0.05) were identified and all differential spots were downregulated in MDR strain J3. Upregulation of chitin binding and BON domain proteins was present in the environmental and some MDR strains, whereas the clinical strains exhibited distinct proteomic profiles with increased expression of serine protein kinase and arginine/ornithine transport ATP-binding proteins. Significant difference in expression was observed between susceptible clinical and MDR strains, as well as susceptible clinical and environmental strains. Transition from an environmental saprophyte to a clinical strain could alter its physiological characteristics to further increase its adaptation.
    MeSH term(s) Cluster Analysis ; Drug Resistance, Multiple, Bacterial ; Environmental Microbiology ; Gene Expression Regulation, Bacterial ; Mass Spectrometry ; Principal Component Analysis ; Proteomics ; Pseudomonas aeruginosa/genetics ; Pseudomonas aeruginosa/metabolism
    Language English
    Publishing date 2021-03-16
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 186544-4
    ISSN 1573-4978 ; 0301-4851
    ISSN (online) 1573-4978
    ISSN 0301-4851
    DOI 10.1007/s11033-021-06262-8
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1140: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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  8. Article ; Online: Natural-derived compounds and their mechanisms in potential autosomal dominant polycystic kidney disease (ADPKD) treatment.

    Mahendran, Rhubaniya / Lim, Soo Kun / Ong, Kien Chai / Chua, Kek Heng / Chai, Hwa Chia

    Clinical and experimental nephrology

    2021  Volume 25, Issue 11, Page(s) 1163–1172

    Abstract: Background: Autosomal dominant polycystic kidney disease (ADPKD) is a monogenic kidney disorder that impairs renal functions progressively leading to kidney failure. The disease affects between 1:400 and 1:1000 ratio of the people worldwide. It is ... ...

    Abstract Background: Autosomal dominant polycystic kidney disease (ADPKD) is a monogenic kidney disorder that impairs renal functions progressively leading to kidney failure. The disease affects between 1:400 and 1:1000 ratio of the people worldwide. It is caused by the mutated PKD1 and PKD2 genes which encode for the defective polycystins. Polycystins mimic the receptor protein or protein channel and mediate aberrant cell signaling that causes cystic development in the renal parenchyma. The cystic development is driven by the increased cyclic AMP stimulating fluid secretion and infinite cell growth. In recent years, natural product-derived small molecules or drugs targeting specific signaling pathways have caught attention in the drug discovery discipline. The advantages of natural products over synthetic drugs enthusiast researchers to utilize the medicinal benefits in various diseases including ADPKD.
    Conclusion: Overall, this review discusses some of the previously studied and reported natural products and their mechanisms of action which may potentially be redirected into ADPKD.
    MeSH term(s) Antioxidants/pharmacology ; Chalcones/pharmacology ; Curcumin/pharmacology ; Diterpenes/pharmacology ; Diterpenes, Kaurane/pharmacology ; Emodin/pharmacology ; Epoxy Compounds/pharmacology ; Estrogen Antagonists/pharmacology ; Flavanones/pharmacology ; Humans ; Hypoglycemic Agents/pharmacology ; Metformin/pharmacology ; Phenanthrenes/pharmacology ; Plant Extracts/pharmacology ; Plant Extracts/therapeutic use ; Polycystic Kidney, Autosomal Dominant/drug therapy ; Protein Kinase Inhibitors/pharmacology ; Quercetin/pharmacology ; Resveratrol/pharmacology
    Chemical Substances Antioxidants ; Chalcones ; Diterpenes ; Diterpenes, Kaurane ; Epoxy Compounds ; Estrogen Antagonists ; Flavanones ; Hypoglycemic Agents ; Phenanthrenes ; Plant Extracts ; Protein Kinase Inhibitors ; triptolide (19ALD1S53J) ; steviol (4741LYX6RT) ; Metformin (9100L32L2N) ; Quercetin (9IKM0I5T1E) ; cardamonin (H8KP1OJ8JX) ; naringenin (HN5425SBF2) ; Curcumin (IT942ZTH98) ; Emodin (KA46RNI6HN) ; Resveratrol (Q369O8926L)
    Language English
    Publishing date 2021-07-12
    Publishing country Japan
    Document type Journal Article ; Review
    ZDB-ID 1338768-6
    ISSN 1437-7799 ; 1342-1751
    ISSN (online) 1437-7799
    ISSN 1342-1751
    DOI 10.1007/s10157-021-02111-x
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4922: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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    Jg. 1995 - 2021: Lesesall (2.OG)
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  9. Article ; Online: Development of insulated isothermal PCR for rapid on-site malaria detection.

    Chua, Kek Heng / Lee, Ping Chin / Chai, Hwa Chia

    Malaria journal

    2016  Volume 15, Page(s) 134

    Abstract: Background: Detection of Plasmodium spp. is sometimes inconvenient especially in rural areas that are distant from a laboratory. In this study a portable diagnostic test of Plasmodium spp. was developed using insulated isothermal polymerase chain ... ...

    Abstract Background: Detection of Plasmodium spp. is sometimes inconvenient especially in rural areas that are distant from a laboratory. In this study a portable diagnostic test of Plasmodium spp. was developed using insulated isothermal polymerase chain reaction (iiPCR) as an alternative approach to improve this situation.
    Methods: A pair of universal primers and probe were designed to amplify and detect gene encoding 18S small sub-unit rRNA of Plasmodium spp using iiPCR method in a portable device, POCKIT™. The efficiency and detection limit of the assay were evaluated using quantitative real-time polymerase chain reaction (qPCR) approach before being subjected to testing in POCKIT™. Detection results of POCKIT™ were displayed as '+', '-' or '?' based on the fluorescence ratio after/before reaction. A total of 55 and 35 samples from malaria patients and healthy subjects, respectively, were screened to evaluate the feasibility of this newly designed iiPCR assay.
    Results: The iiPCR assay allowed the detection of various species of Plasmodium, including those infecting humans (Plasmodium falciparum, P. vivax, P. knowlesi, P. malariae, P. ovale), monkeys, birds, and rodents. Efficiency of the assay achieved 96.9 % while the lower detection limit was ≥100 copies of plasmodial DNA. Specificity of the assay was assured as it could not detect human, bacterial and other parasitic DNA. Among the 55 clinical samples tested, 47 (85.4 %) of them were detected as positive by POCKIT™. Four (7.3 %) samples with fluorescence ratio after/before reaction of <1.2 were reported as negative while another four (7.3 %) were ambiguously detected as they had fluorescence ratios between 1.2 and 1.3. The fluorescence ratio was not found to be associated with the copy number of plasmodial DNA. This approach can only be considered as a qualitative method.
    Conclusions: The portable iiPCR system may serve as an alternative approach for preliminary screening of malaria in endemic rural areas. The system may also be useful for detecting animal malaria in the field. Although it is not as quantitative as qPCR method, it is comparatively fast and easy to handle. It is believed that the POCKIT-iiPCR assay is able to achieve 100 % sensitivity if increased amount of DNA from each sample is used. The iiPCR assay can also be upgraded in future to detect multiple Plasmodium spp. at the same time by designing the specific primers and probes.
    MeSH term(s) DNA Primers/genetics ; DNA, Protozoan/analysis ; DNA, Protozoan/genetics ; Humans ; Malaria/diagnosis ; Parasitology ; Plasmodium/genetics ; Real-Time Polymerase Chain Reaction/methods ; Rural Health Services ; Sensitivity and Specificity
    Chemical Substances DNA Primers ; DNA, Protozoan
    Language English
    Publishing date 2016-03-01
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1475-2875
    ISSN (online) 1475-2875
    DOI 10.1186/s12936-016-1183-z
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  10. Article: Rapid identification of melioidosis agent by an insulated isothermal PCR on a field-deployable device.

    Chua, Kek Heng / Tan, E Wei / Chai, Hwa Chia / Puthucheary, S D / Lee, Ping Chin / Puah, Suat Moi

    PeerJ

    2020  Volume 8, Page(s) e9238

    Abstract: Background: Burkholderia pseudomallei: Method: In this study, we developed a rapid, sensitive and specific insulated isothermal Polymerase Chain Reaction (iiPCR) targeting : Results: All 121 : Conclusion: ... ...

    Abstract Background: Burkholderia pseudomallei
    Method: In this study, we developed a rapid, sensitive and specific insulated isothermal Polymerase Chain Reaction (iiPCR) targeting
    Results: All 121
    Conclusion: This
    Language English
    Publishing date 2020-05-27
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2703241-3
    ISSN 2167-8359
    ISSN 2167-8359
    DOI 10.7717/peerj.9238
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