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  1. Article ; Online: Erector spinae plane block with catheter for management of percutaneous nephrolithotomy: A three case report.

    Resnick, Andrew / Chait, Michael / Landau, Steven / Krishnan, Sandeep

    Medicine

    2020  Volume 99, Issue 40, Page(s) e22477

    Abstract: Introduction: Percutaneous nephrolithotomy is a procedure used for management of refractory renal calculi. Oral and parenteral opioids, along with local anesthetic infiltration, neuraxial anesthesia, and paravertebral blocks are the most common methods ... ...

    Abstract Introduction: Percutaneous nephrolithotomy is a procedure used for management of refractory renal calculi. Oral and parenteral opioids, along with local anesthetic infiltration, neuraxial anesthesia, and paravertebral blocks are the most common methods of managing intra-operative and post-operative pain for these patients. The erector spinae plane block with catheter (ESPC) is a newer interfascial regional anesthetic technique that can be used to manage peri-operative pain in these patients.
    Clinical findings: Three patients complained of significant flank pain were scheduled for percutaneous nephrolithotomy under general anesthesia in the prone position.
    Diagnoses: Patients were diagnosed with large renal calculi.
    Therapeutic interventions: Patients received ESPC in the pre-operative holding area at the level of the T7 transverse process. The ESPCS were bolused with a solution of 30 mL 0.25% bupivacaine with 4 mg dexamethasone prior to surgery. Patients also received oral tramadol 50 mg and acetaminophen 1 g as part of the multimodal pain protocol prior to surgery. After the procedure, the patients were bolused with 0.25% bupivacaine or started on an infusion of 0.25% bupivacaine to manage their pain.
    Outcomes: No opioid or other pain medications, other than the local anesthetic solution given in the ESPCs, were used during the intra-operative or post-operative period for management of pain in these patients. Visual analogue scale (VAS) scores were below 4 for all patients in the post-operative period, and patients did not report any issues with post-operative nausea or vomiting.
    Conclusion: These patients were compared to 3 prior patients who had undergone percutaneous nephrolithotomy without ESPC. The 3 patients without ESPC placement reported increased VAS scores, had increased opioid/pain medication consumption intraoperatively and postoperatively, and had increased incidence of perioperative nausea when compared to our ESPC patients. Our report shows that ESPC, in combination with a multimodal pain protocol, can be a good option for management of patients undergoing percutaneous nephrolithotomy.
    MeSH term(s) Aged ; Anesthetics, Local/therapeutic use ; Bupivacaine/therapeutic use ; Dexamethasone/therapeutic use ; Female ; Humans ; Kidney Calculi/surgery ; Middle Aged ; Nephrolithotomy, Percutaneous/methods ; Nerve Block/methods ; Pain, Postoperative/prevention & control ; Paraspinal Muscles
    Chemical Substances Anesthetics, Local ; Dexamethasone (7S5I7G3JQL) ; Bupivacaine (Y8335394RO)
    Language English
    Publishing date 2020-10-23
    Publishing country United States
    Document type Case Reports ; Journal Article
    ZDB-ID 80184-7
    ISSN 1536-5964 ; 0025-7974
    ISSN (online) 1536-5964
    ISSN 0025-7974
    DOI 10.1097/MD.0000000000022477
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Immune and epithelial determinants of age-related risk and alveolar injury in fatal COVID-19.

    Chait, Michael / Yilmaz, Mine M / Shakil, Shanila / Ku, Amy W / Dogra, Pranay / Connors, Thomas J / Szabo, Peter A / Gray, Joshua I / Wells, Steven B / Kubota, Masaru / Matsumoto, Rei / Poon, Maya Ml / Snyder, Mark E / Baldwin, Matthew R / Sims, Peter A / Saqi, Anjali / Farber, Donna L / Weisberg, Stuart P

    JCI insight

    2022  Volume 7, Issue 11

    Abstract: Respiratory failure in COVID-19 is characterized by widespread disruption of the lung's alveolar gas exchange interface. To elucidate determinants of alveolar lung damage, we performed epithelial and immune cell profiling in lungs from 24 COVID-19 ... ...

    Abstract Respiratory failure in COVID-19 is characterized by widespread disruption of the lung's alveolar gas exchange interface. To elucidate determinants of alveolar lung damage, we performed epithelial and immune cell profiling in lungs from 24 COVID-19 autopsies and 43 uninfected organ donors ages 18-92 years. We found marked loss of type 2 alveolar epithelial (T2AE) cells and increased perialveolar lymphocyte cytotoxicity in all fatal COVID-19 cases, even at early stages before typical patterns of acute lung injury are histologically apparent. In lungs from uninfected organ donors, there was also progressive loss of T2AE cells with increasing age, which may increase susceptibility to COVID-19-mediated lung damage in older individuals. In the fatal COVID-19 cases, macrophage infiltration differed according to the histopathological pattern of lung injury. In cases with acute lung injury, we found accumulation of CD4+ macrophages that expressed distinctly high levels of T cell activation and costimulation genes and strongly correlated with increased extent of alveolar epithelial cell depletion and CD8+ T cell cytotoxicity. Together, our results show that T2AE cell deficiency may underlie age-related COVID-19 risk and initiate alveolar dysfunction shortly after infection, and we define immune cell mediators that may contribute to alveolar injury in distinct pathological stages of fatal COVID-19.
    MeSH term(s) Acute Lung Injury/pathology ; Adolescent ; Adult ; Aged ; Aged, 80 and over ; Alveolar Epithelial Cells/pathology ; Autopsy ; COVID-19 ; Humans ; Lung/pathology ; Middle Aged ; Young Adult
    Language English
    Publishing date 2022-06-08
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, N.I.H., Extramural
    ISSN 2379-3708
    ISSN (online) 2379-3708
    DOI 10.1172/jci.insight.157608
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Routine colonoscopy, diabetic eye care, mammogram and pap smear screening in vascular surgery patients.

    Lee, Young / Aurshina, Afsha / Lee, Aaron J / Ackerman, Israel M / Chait, Michael / Novak, Daniel / Hingorani, Anil / Ascher, Enrico / Marks, Natalie

    Vascular

    2017  Volume 26, Issue 4, Page(s) 372–377

    Abstract: Objective An increasing emphasis on preventive medicine has been supported by the recent reforms in United States health care system. Majority of the patients seen in vascular surgery clinics are elderly with more extensive medical comorbidities compared ...

    Abstract Objective An increasing emphasis on preventive medicine has been supported by the recent reforms in United States health care system. Majority of the patients seen in vascular surgery clinics are elderly with more extensive medical comorbidities compared to the general population. Thus, these patients would be expected at higher risk for common malignant pathologies such as colon, breast and cervical cancer, and nonmalignant diseases such as diabetic retinopathy. This study looked at the screening compliance of vascular patients compared to data provided by Centers for Disease Control on the national and state levels. Methods The office records of 851 consecutive patients seen in Brooklyn and Staten Island vascular clinics were examined. We queried patients regarding their last colonoscopy, diabetic eye exams, recent mammograms, and Pap smears. Our patient screening compliance was compared between the two clinics as well as to the national and New York state data provided by Centers for Disease Control. Compliance with regard to patient's age was also examined. Results Patients referred to the Staten Island office have a better colonoscopy compliance compared to the Brooklyn office ( P = .0001) and the national Centers for Disease Control average ( P = .026). Compliance for mammography and cervical cancer screening was higher in Staten Island office compared to the Brooklyn office ( P = .0001, P < .0001), respectively. Compliance was lower for Pap smear ( P = .0273) in Brooklyn when compared to the national average. Compliance for colonoscopy increased with age for both clinics ( P = .001, P < .001), while Pap smear decreased ( P < .001, P = .004). Conclusion Patients in vascular clinics in an urban setting had better adherence to screening protocol than the national and state average, with the exception of female patients for colonoscopy in our Brooklyn vascular office. There exists variability in both patient populations based on sub-specific locality and demographics including socioeconomic status. Overall, however patients in Staten Island had better compliance and adherence to the screening protocol than Brooklyn vascular clinic.
    MeSH term(s) Colonoscopy/trends ; Colonoscopy/utilization ; Diabetic Retinopathy/diagnosis ; Diagnostic Techniques, Ophthalmological/trends ; Diagnostic Techniques, Ophthalmological/utilization ; Female ; Guideline Adherence ; Healthcare Disparities/trends ; Humans ; Male ; Mammography/trends ; Mammography/utilization ; New York ; Office Visits/trends ; Papanicolaou Test/trends ; Papanicolaou Test/utilization ; Patient Compliance ; Practice Guidelines as Topic ; Practice Patterns, Physicians'/trends ; Urban Health Services/trends ; Vascular Surgical Procedures
    Language English
    Publishing date 2017-11-19
    Publishing country England
    Document type Comparative Study ; Journal Article ; Multicenter Study
    ZDB-ID 2137151-9
    ISSN 1708-539X ; 1708-5381
    ISSN (online) 1708-539X
    ISSN 1708-5381
    DOI 10.1177/1708538117742830
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Longitudinal profiling of respiratory and systemic immune responses reveals myeloid cell-driven lung inflammation in severe COVID-19.

    Szabo, Peter A / Dogra, Pranay / Gray, Joshua I / Wells, Steven B / Connors, Thomas J / Weisberg, Stuart P / Krupska, Izabela / Matsumoto, Rei / Poon, Maya M L / Idzikowski, Emma / Morris, Sinead E / Pasin, Chloé / Yates, Andrew J / Ku, Amy / Chait, Michael / Davis-Porada, Julia / Guo, Xinzheng V / Zhou, Jing / Steinle, Matthew /
    Mackay, Sean / Saqi, Anjali / Baldwin, Matthew R / Sims, Peter A / Farber, Donna L

    Immunity

    2021  Volume 54, Issue 4, Page(s) 797–814.e6

    Abstract: Immune response dynamics in coronavirus disease 2019 (COVID-19) and their severe manifestations have largely been studied in circulation. Here, we examined the relationship between immune processes in the respiratory tract and circulation through ... ...

    Abstract Immune response dynamics in coronavirus disease 2019 (COVID-19) and their severe manifestations have largely been studied in circulation. Here, we examined the relationship between immune processes in the respiratory tract and circulation through longitudinal phenotypic, transcriptomic, and cytokine profiling of paired airway and blood samples from patients with severe COVID-19 relative to heathy controls. In COVID-19 airways, T cells exhibited activated, tissue-resident, and protective profiles; higher T cell frequencies correlated with survival and younger age. Myeloid cells in COVID-19 airways featured hyperinflammatory signatures, and higher frequencies of these cells correlated with mortality and older age. In COVID-19 blood, aberrant CD163
    MeSH term(s) Adolescent ; Adult ; Age Factors ; Aged ; Aged, 80 and over ; COVID-19/blood ; COVID-19/immunology ; COVID-19/mortality ; COVID-19/pathology ; Cytokines/immunology ; Cytokines/metabolism ; Humans ; Inflammation ; Longitudinal Studies ; Lung/immunology ; Lung/pathology ; Macrophages/immunology ; Macrophages/pathology ; Middle Aged ; Monocytes/immunology ; Monocytes/pathology ; Myeloid Cells/immunology ; Myeloid Cells/pathology ; SARS-CoV-2 ; T-Lymphocytes/immunology ; T-Lymphocytes/pathology ; Transcriptome ; Young Adult
    Chemical Substances Cytokines
    Language English
    Publishing date 2021-03-11
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 1217235-2
    ISSN 1097-4180 ; 1074-7613
    ISSN (online) 1097-4180
    ISSN 1074-7613
    DOI 10.1016/j.immuni.2021.03.005
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Tissue-Resident Memory T Cells Mediate Immune Homeostasis in the Human Pancreas through the PD-1/PD-L1 Pathway.

    Weisberg, Stuart P / Carpenter, Dustin J / Chait, Michael / Dogra, Pranay / Gartrell-Corrado, Robyn D / Chen, Andrew X / Campbell, Sean / Liu, Wei / Saraf, Pooja / Snyder, Mark E / Kubota, Masaru / Danzl, Nichole M / Schrope, Beth A / Rabadan, Raul / Saenger, Yvonne / Chen, Xiaojuan / Farber, Donna L

    Cell reports

    2019  Volume 29, Issue 12, Page(s) 3916–3932.e5

    Abstract: Non-recirculating tissue-resident memory T cells (TRMs) are the predominant T cell subset in diverse tissue sites, where they mediate protective immune responses in situ. Here, we reveal a role for TRM in maintaining immune homeostasis in the human ... ...

    Abstract Non-recirculating tissue-resident memory T cells (TRMs) are the predominant T cell subset in diverse tissue sites, where they mediate protective immune responses in situ. Here, we reveal a role for TRM in maintaining immune homeostasis in the human pancreas through interactions with resident macrophages and the PD-1/PD-L1 inhibitory pathway. Using tissues obtained from organ donors, we identify that pancreas T cells comprise CD8
    MeSH term(s) B7-H1 Antigen/genetics ; B7-H1 Antigen/metabolism ; CD58 Antigens/metabolism ; Humans ; Immunity, Mucosal/genetics ; Immunity, Mucosal/physiology ; Immunologic Memory/genetics ; Immunologic Memory/physiology ; Macrophages/immunology ; Macrophages/metabolism ; Pancreas/immunology ; Pancreas/metabolism ; Pancreas/pathology ; Pancreatitis/genetics ; Pancreatitis/immunology ; Pancreatitis/metabolism ; Programmed Cell Death 1 Receptor/genetics ; Programmed Cell Death 1 Receptor/metabolism ; Signal Transduction ; T-Lymphocyte Subsets/immunology ; T-Lymphocyte Subsets/metabolism
    Chemical Substances B7-H1 Antigen ; CD274 protein, human ; CD58 Antigens ; PDCD1 protein, human ; Programmed Cell Death 1 Receptor
    Language English
    Publishing date 2019-12-18
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 2649101-1
    ISSN 2211-1247 ; 2211-1247
    ISSN (online) 2211-1247
    ISSN 2211-1247
    DOI 10.1016/j.celrep.2019.11.056
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: Analysis of respiratory and systemic immune responses in COVID-19 reveals mechanisms of disease pathogenesis.

    Szabo, Peter A / Dogra, Pranay / Gray, Joshua I / Wells, Steven B / Connors, Thomas J / Weisberg, Stuart P / Krupska, Izabela / Matsumoto, Rei / Poon, Maya M L / Idzikowski, Emma / Morris, Sinead E / Pasin, Chloé / Yates, Andrew J / Ku, Amy / Chait, Michael / Davis-Porada, Julia / Zhou, Jing / Steinle, Matthew / Mackay, Sean /
    Saqi, Anjali / Baldwin, Matthew / Sims, Peter A / Farber, Donna L

    medRxiv : the preprint server for health sciences

    2020  

    Abstract: Immune responses to respiratory viruses like SARS-CoV-2 originate and function in the lung, yet assessments of human immunity are often limited to blood. Here, we conducted longitudinal, high-dimensional profiling of paired airway and blood samples from ... ...

    Abstract Immune responses to respiratory viruses like SARS-CoV-2 originate and function in the lung, yet assessments of human immunity are often limited to blood. Here, we conducted longitudinal, high-dimensional profiling of paired airway and blood samples from patients with severe COVID-19, revealing immune processes in the respiratory tract linked to disease pathogenesis. Survival from severe disease was associated with increased CD4
    Keywords covid19
    Language English
    Publishing date 2020-10-18
    Publishing country United States
    Document type Preprint
    DOI 10.1101/2020.10.15.20208041
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article: Antibody responses to SARS-CoV2 are distinct in children with MIS-C compared to adults with COVID-19.

    Weisberg, Stuart P / Connors, Thomas / Zhu, Yun / Baldwin, Matthew / Lin, Wen-Hsuan / Wontakal, Sandeep / Szabo, Peter A / Wells, Steven B / Dogra, Pranay / Gray, Joshua I / Idzikowski, Emma / Bovier, Francesca / Davis-Porada, Julia / Matsumoto, Rei / Li Poon, Maya Meimei / Chait, Michael P / Mathieu, Cyrille / Horvat, Branka / Decimo, Didier /
    Bitan, Zachary C / La Carpia, Francesca / Ferrara, Stephen A / Mace, Emily / Milner, Joshua / Moscona, Anne / Hod, Eldad A / Porotto, Matteo / Farber, Donna L

    medRxiv : the preprint server for health sciences

    2020  

    Abstract: Clinical manifestations of COVID-19 caused by the novel coronavirus SARS-CoV-2 are associated with age. While children are largely spared from severe respiratory disease, they can present with a SARS-CoV-2-associated multisystem inflammatory syndrome ( ... ...

    Abstract Clinical manifestations of COVID-19 caused by the novel coronavirus SARS-CoV-2 are associated with age. While children are largely spared from severe respiratory disease, they can present with a SARS-CoV-2-associated multisystem inflammatory syndrome (MIS-C) similar to Kawasaki's disease. Here, we show distinct antibody (Ab) responses in children with MIS-C compared to adults with severe COVID-19 causing acute respiratory distress syndrome (ARDS), and those who recovered from mild disease. There was a reduced breadth and specificity of anti-SARS-CoV-2-specific antibodies in MIS-C patients compared to the COVID patient groups; MIS-C predominantly generated IgG Abs specific for the Spike (S) protein but not for the nucleocapsid (N) protein, while both COVID-19 cohorts had anti-S IgG, IgM and IgA Abs, as well as anti-N IgG Abs. Moreover, MIS-C patients had reduced neutralizing activity compared to COVID-19 cohorts, indicating a reduced protective serological response. These results suggest a distinct infection course and immune response in children and adults who develop severe disease, with implications for optimizing treatments based on symptom and age.
    Keywords covid19
    Language English
    Publishing date 2020-07-14
    Publishing country United States
    Document type Preprint
    DOI 10.1101/2020.07.12.20151068
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Distinct antibody responses to SARS-CoV-2 in children and adults across the COVID-19 clinical spectrum.

    Weisberg, Stuart P / Connors, Thomas J / Zhu, Yun / Baldwin, Matthew R / Lin, Wen-Hsuan / Wontakal, Sandeep / Szabo, Peter A / Wells, Steven B / Dogra, Pranay / Gray, Joshua / Idzikowski, Emma / Stelitano, Debora / Bovier, Francesca T / Davis-Porada, Julia / Matsumoto, Rei / Poon, Maya Meimei Li / Chait, Michael / Mathieu, Cyrille / Horvat, Branka /
    Decimo, Didier / Hudson, Krystalyn E / Zotti, Flavia Dei / Bitan, Zachary C / La Carpia, Francesca / Ferrara, Stephen A / Mace, Emily / Milner, Joshua / Moscona, Anne / Hod, Eldad / Porotto, Matteo / Farber, Donna L

    Nature immunology

    2020  Volume 22, Issue 1, Page(s) 25–31

    Abstract: Clinical manifestations of COVID-19 caused by the new coronavirus SARS-CoV-2 are associated with ... ...

    Abstract Clinical manifestations of COVID-19 caused by the new coronavirus SARS-CoV-2 are associated with age
    MeSH term(s) Adolescent ; Adult ; Aged ; Antibodies, Viral/immunology ; Antibody Formation/immunology ; COVID-19/immunology ; COVID-19/virology ; Child ; Child, Preschool ; Female ; Humans ; Immunoglobulin A/immunology ; Immunoglobulin G/immunology ; Immunoglobulin M/immunology ; Male ; Middle Aged ; Nucleocapsid Proteins/immunology ; SARS-CoV-2/immunology ; SARS-CoV-2/physiology ; Spike Glycoprotein, Coronavirus/immunology ; Young Adult
    Chemical Substances Antibodies, Viral ; Immunoglobulin A ; Immunoglobulin G ; Immunoglobulin M ; Nucleocapsid Proteins ; Spike Glycoprotein, Coronavirus ; spike protein, SARS-CoV-2
    Keywords covid19
    Language English
    Publishing date 2020-11-05
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 2016987-5
    ISSN 1529-2916 ; 1529-2908
    ISSN (online) 1529-2916
    ISSN 1529-2908
    DOI 10.1038/s41590-020-00826-9
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Analysis of respiratory and systemic immune responses in COVID-19 reveals mechanisms of disease pathogenesis

    Szabo, Peter A. / Dogra, Pranay / Gray, Joshua I. / Wells, Steven B. / Connors, Thomas J. / Weisberg, Stuart P. / Krupska, Izabela / Matsumoto, Rei / Poon, Maya M.L. / Idzikowski, Emma / Morris, Sinead E. / Chloe, Pasin / Yates, Andrew J. / Ku, Amy / Chait, Michael / Davis-Porada, Julia M. / Zhou, Jing / Steinle, Matthew / Mackay, Sean /
    Saqi, Anjali / Baldwin, Matthew R. / Sims, Peter A. / Farber, Donna L

    medRxiv

    Abstract: Immune responses to respiratory viruses like SARS-CoV-2 originate and function in the lung, yet assessments of human immunity are often limited to blood. Here, we conducted longitudinal, high-dimensional profiling of paired airway and blood samples from ... ...

    Abstract Immune responses to respiratory viruses like SARS-CoV-2 originate and function in the lung, yet assessments of human immunity are often limited to blood. Here, we conducted longitudinal, high-dimensional profiling of paired airway and blood samples from patients with severe COVID-19, revealing immune processes in the respiratory tract linked to disease pathogenesis. Survival from severe disease was associated with increased CD4+T cells and decreased monocyte/macrophage frequencies in the airway, but not in blood. Airway T cells and macrophages exhibited tissue-resident phenotypes and activation signatures, including high level expression and secretion of monocyte chemoattractants CCL2 and CCL3 by airway macrophages. By contrast, monocytes in blood expressed the CCL2-receptor CCR2 and aberrant CD163+ and immature phenotypes. Extensive accumulation of CD163+monocyte/macrophages within alveolar spaces in COVID-19 lung autopsies suggested recruitment from circulation. Our findings provide evidence that COVID-19 pathogenesis is driven by respiratory immunity, and rationale for site-specific treatment and prevention strategies.
    Keywords covid19
    Language English
    Publishing date 2020-10-19
    Publisher Cold Spring Harbor Laboratory Press
    Document type Article ; Online
    DOI 10.1101/2020.10.15.20208041
    Database COVID19

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  10. Article ; Online: Antibody responses to SARS-CoV2 are distinct in children with MIS-C compared to adults with COVID-19

    Weisberg, Stuart P / Connors, Thomas / Zhu, Yun / Baldwin, Matthew / Lin, Wen-Hsuan / Wontakal, Sandeep / Szabo, Peter A / Wells, Steven B / Dogra, Pranay / Gray, Joshua I / Idzikowski, Emma / Bovier, Francesca / Davis-Porada, Julia / Matsumoto, Rei / Li Poon, Maya Meimei / Chait, Michael P / Mathieu, Cyrille / Horvat, Branka / Decimo, Didier /
    Bitan, Zachary C / La Carpia, Francesca / Ferrara, Stephen A / Mace, Emily / Milner, Joshua / Moscona, Anne / Hod, Eldad A / Porotto, Matteo / Farber, Donna L

    medRxiv

    Abstract: Clinical manifestations of COVID-19 caused by the novel coronavirus SARS-CoV-2 are associated with age. While children are largely spared from severe respiratory disease, they can present with a SARS-CoV-2-associated multisystem inflammatory syndrome ( ... ...

    Abstract Clinical manifestations of COVID-19 caused by the novel coronavirus SARS-CoV-2 are associated with age. While children are largely spared from severe respiratory disease, they can present with a SARS-CoV-2-associated multisystem inflammatory syndrome (MIS-C) similar to Kawasaki9s disease. Here, we show distinct antibody (Ab) responses in children with MIS-C compared to adults with severe COVID-19 causing acute respiratory distress syndrome (ARDS), and those who recovered from mild disease. There was a reduced breadth and specificity of anti-SARS-CoV-2-specific antibodies in MIS-C patients compared to the COVID patient groups; MIS-C predominantly generated IgG Abs specific for the Spike (S) protein but not for the nucleocapsid (N) protein, while both COVID-19 cohorts had anti-S IgG, IgM and IgA Abs, as well as anti-N IgG Abs. Moreover, MIS-C patients had reduced neutralizing activity compared to COVID-19 cohorts, indicating a reduced protective serological response. These results suggest a distinct infection course and immune response in children and adults who develop severe disease, with implications for optimizing treatments based on symptom and age.
    Keywords covid19
    Language English
    Publishing date 2020-07-14
    Publisher Cold Spring Harbor Laboratory Press
    Document type Article ; Online
    DOI 10.1101/2020.07.12.20151068
    Database COVID19

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