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  1. Article ; Online: Rare occurrence of Coxsackievirus B4 along with Coxsackieviruses A6 and A10 from hand, foot and mouth disease

    Sampada Tambolkar / Manasi Chavan / Prachi Prasad / Avinash Lendave / Chandrashekhar Raut / Sharad Agarkhedkar / Jitendra Bhawalkar

    Journal of Krishna Institute of Medical Sciences University, Vol 12, Iss 03, Pp 3-

    2023  Volume 11

    Abstract: Background: Hand, Foot and Mouth Disease (HFMD) has been widely spread in Asia and resulted in a high disease burden among children in many countries. It remains a common problem in India, yet its etiology is largely unknown as diagnosis is based on ... ...

    Abstract Background: Hand, Foot and Mouth Disease (HFMD) has been widely spread in Asia and resulted in a high disease burden among children in many countries. It remains a common problem in India, yet its etiology is largely unknown as diagnosis is based on clinical characteristics. Aim and Objectives: To investigate the circulation of strains, clinical, molecular and virological aspects of the disease. Material and Methods: Vesicular lesion and throat swab specimens were referred from eight children for laboratory diagnosis of HFMD. The detection of enteroviruses was performed by nested PCR, virus isolation using HeLa cell line and sequencing of 5′UTR. Results: Coxsackieviruses A6, A10 and B4 were identified by nested PCR, isolated in HeLa cell line and reconfirmed. The phylogenetic analysis of Coxsackieviruses A6, A10 and B4 showed similarity with Beijing-China, Pune-India, and France respectively. Conclusion: The findings of this study indicate the co-circulation of Coxsackieviruses A6, A10 and B4. Coxsackievirus B4 was isolated and detected for the first time from Pune, India. Additionally, results emphasize the importance of virus isolation using cell lines for laboratory diagnosis. Point-of-Care testing and vaccine development would be beneficial towards control and prevention of HFMD.
    Keywords coxsackievirus b4 ; enteroviruses ; hand ; foot and mouth disease ; Medicine ; R ; Medicine (General) ; R5-920
    Subject code 610
    Language English
    Publishing date 2023-07-01T00:00:00Z
    Publisher Krishna Institute of Medical Sciences University
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Presence of SARS-CoV-2 in human tears detected by quantitative real time PCR (qRT-PCR)

    Neeta Mishra / Chandrashekhar Raut / Neelam Prasad / J S Bhawalkar / Safia Farooqui / Shahzad Mirza / Veshal V Madan

    Medical Journal of Dr. D.Y. Patil Vidyapeeth, Vol 15, Iss 7, Pp 46-

    2022  Volume 48

    Abstract: Background: COVID-19 has caused a pandemic since the end of the year 2019. Controversy regarding the presence of the SARS-CoV-2 in tears and conjunctival sac has created suspense throughout. Moderate to severe dry eye has been observed in the patients ... ...

    Abstract Background: COVID-19 has caused a pandemic since the end of the year 2019. Controversy regarding the presence of the SARS-CoV-2 in tears and conjunctival sac has created suspense throughout. Moderate to severe dry eye has been observed in the patients infected with COVID-19. Material and Method: We conducted a descriptive, prospective observational study. We enrolled 16 patients fulfilling the inclusion criteria for our study. Patients affected with acute COVID-19 from 28-9-2020 to 9-10-2020 registered at our hospital and willing to participate were included. Tears and conjunctival swabs were taken at the interval of 24 h; consecutively three samples were taken from one COVID-19 infected patient. In total, 48 samples were analyzed by qRT-PCR. Result: We had 16 participants, 47% were females and 53% were males; 2/16 [12.5%] patients showed the presence of SARS-CoV-2 in tears and conjunctival swabs. Both the infected patients were females, and they did not have any obvious abnormal ocular conditions. Schirmer strip reading, however, showed a severe dry eye in both the patients. Conclusion: We identified dry eye ranging from mild to moderate to severe [Schirmer 1 reading ranged from 2 to 8 mm] in all our patients admitted to the COVID-19 ward. The SARS-CoV-2 gene seems to be mutating. More work needs to be done to find the viral load in the tears and any abnormalities in the eyes accordingly.
    Keywords covid -19 ; qrt-pcr ; schirmer strip ; Medicine ; R
    Subject code 610 ; 616
    Language English
    Publishing date 2022-01-01T00:00:00Z
    Publisher Wolters Kluwer Medknow Publications
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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