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  1. Article: Synthesis and Properties of Nitrogen-Doped Carbon Quantum Dots Using Lactic Acid as Carbon Source.

    Chang, Kaixin / Zhu, Qianjin / Qi, Liyan / Guo, Mingwei / Gao, Woming / Gao, Qinwei

    Materials (Basel, Switzerland)

    2022  Volume 15, Issue 2

    Abstract: Nitrogen-doped carbon quantum dots (N-CQDs) were synthesized in a one-step hydrothermal technique utilizing L-lactic acid as that of the source of carbon and ethylenediamine as that of the source of nitrogen, and were characterized using dynamic light ... ...

    Abstract Nitrogen-doped carbon quantum dots (N-CQDs) were synthesized in a one-step hydrothermal technique utilizing L-lactic acid as that of the source of carbon and ethylenediamine as that of the source of nitrogen, and were characterized using dynamic light scattering, X-ray photoelectron spectroscopy ultraviolet-visible spectrum, Fourier-transformed infrared spectrum, high-resolution transmission electron microscopy, and fluorescence spectrum. The generated N-CQDs have a spherical structure and overall diameters ranging from 1-4 nm, and their surface comprises specific functional groups such as amino, carboxyl, and hydroxyl, resulting in greater water solubility and fluorescence. The quantum yield of N-CQDs (being 46%) is significantly higher than that of the CQDs synthesized from other biomass in literatures. Its fluorescence intensity is dependent on the excitation wavelength, and N-CQDs release blue light at 365 nm under ultraviolet light. The pH values may impact the protonation of N-CQDs surface functional groups and lead to significant fluorescence quenching of N-CQDs. Therefore, the fluorescence intensity of N-CQDs is the highest at pH 7.0, but it decreases with pH as pH values being either more than or less than pH 7.0. The N-CQDs exhibit high sensitivity to Fe
    Language English
    Publishing date 2022-01-08
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2487261-1
    ISSN 1996-1944
    ISSN 1996-1944
    DOI 10.3390/ma15020466
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Monounsaturated fatty acid-enriched olive oil exacerbates chronic alcohol-induced hepatic steatosis and liver injury in C57BL/6J mice.

    Guo, Rui / Chen, Lin / Zhu, Jinyan / Li, Jiaomei / Ding, Qingchao / Chang, Kaixin / Han, Qiang / Li, Songtao

    Food & function

    2023  Volume 14, Issue 3, Page(s) 1573–1583

    Abstract: Dietary oil composition determines the pathological processes of alcoholic fatty liver disease (AFLD). Oil rich in saturated fatty acids protects, whereas oil rich in polyunsaturated fatty acids aggravates the alcohol-induced liver injury. However, ... ...

    Abstract Dietary oil composition determines the pathological processes of alcoholic fatty liver disease (AFLD). Oil rich in saturated fatty acids protects, whereas oil rich in polyunsaturated fatty acids aggravates the alcohol-induced liver injury. However, limited studies have been conducted to address how monounsaturated fatty acids (MUFAs) enriched oil controls the pathological development of AFLD. Therefore, this study was designed to evaluate the effect of MUFA-enriched extra virgin olive oil (OO) on AFLD. Twenty C57BL/6J mice were randomly allocated into four groups and fed modified Lieber-DeCarli liquid diets containing isocaloric maltose dextrin a non-alcohol or alcohol with corn oil and OO for four weeks. Dietary OO significantly exacerbated alcohol-induced liver dysfunction, evidenced by histological examinations and disturbed biochemical parameters. Dietary OO with alcohol decreased hormone-sensitive lipase (HSL), phosphorylated 5'-AMP-activated protein kinase (p-AMPK), and carnitine palmitoyltransferase-Iα (CPT1α) expression, and increased sterol regulatory element-binding protein-1c (SREBP-1c), diacylglycerol acyltransferase-2 (DGAT2), and very low-density lipoprotein receptor (VLDLR) expression in the liver. It also promoted the expression of hepatic interleukin-6 (
    MeSH term(s) Mice ; Animals ; Olive Oil/pharmacology ; Fatty Liver, Alcoholic/etiology ; Fatty Liver, Alcoholic/metabolism ; Fatty Acids, Monounsaturated/metabolism ; Mice, Inbred C57BL ; Liver/metabolism ; Ethanol/metabolism ; Fatty Acids/metabolism ; Corn Oil/metabolism
    Chemical Substances Olive Oil ; Fatty Acids, Monounsaturated ; Ethanol (3K9958V90M) ; Fatty Acids ; Corn Oil (8001-30-7)
    Language English
    Publishing date 2023-02-06
    Publishing country England
    Document type Journal Article
    ZDB-ID 2612033-1
    ISSN 2042-650X ; 2042-6496
    ISSN (online) 2042-650X
    ISSN 2042-6496
    DOI 10.1039/d2fo03323b
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: 1-Methylnicotinamide promotes hepatic steatosis in mice: A potential mechanism in chronic alcohol-induced fatty liver disease.

    Lai, Shanglei / Ma, Yue / Hao, Liuyi / Ding, Qinchao / Chang, Kaixin / Zhuge, Hui / Qiu, Jiannan / Xu, Tiantian / Dou, Xiaobing / Li, Songtao

    Biochimica et biophysica acta. Molecular and cell biology of lipids

    2023  Volume 1868, Issue 4, Page(s) 159286

    Abstract: Alcohol abuse and its related diseases are the major risk factors for human health. Alcohol-related liver disease (ALD) is a leading cause of morbidity and mortality worldwide. Although the mechanism of ALD has been widely investigated, liver metabolites ...

    Abstract Alcohol abuse and its related diseases are the major risk factors for human health. Alcohol-related liver disease (ALD) is a leading cause of morbidity and mortality worldwide. Although the mechanism of ALD has been widely investigated, liver metabolites associated with long-term alcohol intake-induced hepatic steatosis have not been well explored. In this study, we aimed to investigate the role and mechanisms of 1-methylnicotinamide (1-MNA), a metabolite during nicotinamide adenine dinucleotide (NAD
    MeSH term(s) Animals ; Mice ; Chronic Disease ; Ethanol/adverse effects ; Fatty Liver/chemically induced ; Fatty Liver, Alcoholic/genetics ; Mice, Inbred C57BL ; Sterol Regulatory Element Binding Protein 1/genetics ; Sterol Regulatory Element Binding Protein 1/metabolism
    Chemical Substances Ethanol (3K9958V90M) ; N(1)-methylnicotinamide (UM47085BXC) ; Sterol Regulatory Element Binding Protein 1
    Language English
    Publishing date 2023-01-21
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 60-7
    ISSN 1879-2618 ; 1879-2596 ; 1879-260X ; 1872-8006 ; 1879-2642 ; 1879-2650 ; 0006-3002 ; 0005-2728 ; 0005-2736 ; 0304-4165 ; 0167-4838 ; 1388-1981 ; 0167-4889 ; 0167-4781 ; 0304-419X ; 1570-9639 ; 0925-4439 ; 1874-9399
    ISSN (online) 1879-2618 ; 1879-2596 ; 1879-260X ; 1872-8006 ; 1879-2642 ; 1879-2650
    ISSN 0006-3002 ; 0005-2728 ; 0005-2736 ; 0304-4165 ; 0167-4838 ; 1388-1981 ; 0167-4889 ; 0167-4781 ; 0304-419X ; 1570-9639 ; 0925-4439 ; 1874-9399
    DOI 10.1016/j.bbalip.2023.159286
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: The micro-743a-3p-GSTM1 pathway is an endogenous protective mechanism against alcohol-related liver disease in mice.

    Xu, Tiantian / Pan, Yan / Ding, Qinchao / Cao, Feiwei / Chang, Kaixin / Qiu, Jiannan / Zhuge, Hui / Hao, Liuyi / Wei, Haibin / Si, Caijuan / Dou, Xiaobing / Li, Songtao

    Cellular & molecular biology letters

    2024  Volume 29, Issue 1, Page(s) 35

    Abstract: Background and aims: Epidemiological evidence suggests that the phenotype of glutathione S-transferase mu 1 (GSTM1), a hepatic high-expressed phase II detoxification enzyme, is closely associated with the incidence of alcohol-related liver disease (ALD). ...

    Abstract Background and aims: Epidemiological evidence suggests that the phenotype of glutathione S-transferase mu 1 (GSTM1), a hepatic high-expressed phase II detoxification enzyme, is closely associated with the incidence of alcohol-related liver disease (ALD). However, whether and how hepatic GSTM1 determines the development of ALD is largely unclear. This study was designed to elucidate the role and potential mechanism(s) of hepatic GSTM1 in the pathological process of ALD.
    Methods: GSTM1 was detected in the liver of various ALD mice models and cultured hepatocytes. Liver-specific GSTM1 or/and micro (miR)-743a-3p deficiency mice were generated by adenoassociated virus-8 delivered shRNA, respectively. The potential signal pathways involving in alcohol-regulated GSTM1 and GSTM1-associated ALD were explored via both genetic manipulation and pharmacological approaches.
    Results: GSTM1 was significantly upregulated in both chronic alcohol-induced mice liver and ethanol-exposed murine primary hepatocytes. Alcohol-reduced miR-743a-3p directly contributed to the upregulation of GSTM1, since liver specific silencing miR-743a-3p enhanced GSTM1 and miR-743a-3p loss protected alcohol-induced liver dysfunctions, which was significantly blocked by GSTM1 knockdown. GSTM1 loss robustly aggravated alcohol-induced hepatic steatosis, oxidative stress, inflammation, and early fibrotic-like changes, which was associated with the activation of apoptosis signal-regulating kinase 1 (ASK1), c-Jun N-terminal kinase (JNK), and p38. GSTM1 antagonized ASK1 phosphorylation and its downstream JNK/p38 signaling pathway upon chronic alcohol consumption via binding with ASK1. ASK1 blockage significantly rescued hepatic GSTM1 loss-enhanced disorders in alcohol-fed mice liver.
    Conclusions: Chronic alcohol consumption-induced upregulation of GSTM1 in the liver provides a feedback protection against hepatic steatosis and liver injury by counteracting ASK1 activation. Down-regulation of miR-743a-3p improves alcohol intake-induced hepatic steatosis and liver injury via direct targeting on GSTM1. The miR-743a-3p-GSTM1 axis functions as an innate protective pathway to defend the early stage of ALD.
    MeSH term(s) Animals ; Mice ; Glutathione Transferase/metabolism ; Hepatocytes/metabolism ; Hepatocytes/pathology ; Liver/pathology ; MicroRNAs/metabolism ; Fatty Liver, Alcoholic/metabolism
    Chemical Substances Glutathione Transferase (EC 2.5.1.18) ; MicroRNAs ; Mirn743 microRNA, mouse ; glutathione S-transferase M1 (EC 2.5.1.18)
    Language English
    Publishing date 2024-03-12
    Publishing country England
    Document type Journal Article
    ZDB-ID 2108724-6
    ISSN 1689-1392 ; 1689-1392
    ISSN (online) 1689-1392
    ISSN 1689-1392
    DOI 10.1186/s11658-024-00557-x
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Xie Zhuo Tiao Zhi formula ameliorates chronic alcohol-induced liver injury in mice.

    Chang, Kaixin / Guo, Rui / Hu, Wenbo / Wang, Xuezhu / Cao, Feiwei / Qiu, Jiannan / Li, Jiaomei / Han, Qiang / Du, Zhongyan / Dou, Xiaobing / Li, Songtao

    Frontiers in pharmacology

    2024  Volume 15, Page(s) 1363131

    Abstract: This study aimed to evaluate the protective role and potential mechanisms of Xie Zhuo Tiao Zhi decoction (XZTZ) on alcohol-associated liver disease (ALD). XZTZ significantly alleviated alcohol-induced liver dysfunction, based on histological examinations ...

    Abstract This study aimed to evaluate the protective role and potential mechanisms of Xie Zhuo Tiao Zhi decoction (XZTZ) on alcohol-associated liver disease (ALD). XZTZ significantly alleviated alcohol-induced liver dysfunction, based on histological examinations and biochemical parameters after 4-week administration. Mechanically, alcohol-stimulated hepatic oxidative stress was ameliorated by XZTZ, accompanied by the improvement of Nrf2/Keap1 expression and alcohol-activated phosphorylation of pro-inflammatory transcription factors, including JNK, P38, P65, and IκBα, were rescued by XZTZ. In conclusion, XZTZ demonstrates potential in alleviating alcohol-induced liver injury, oxidative stress, and inflammation possibly through modulation of Nrf2/Keap1 and MAPKs/NF-κB signaling pathways, suggesting its potential as a therapeutic option for patients with alcoholic liver disease.
    Language English
    Publishing date 2024-04-12
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2587355-6
    ISSN 1663-9812
    ISSN 1663-9812
    DOI 10.3389/fphar.2024.1363131
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Surface Modification of Poly(l-lactic acid) through Stereocomplexation with Enantiomeric Poly(d-lactic acid) and Its Copolymer.

    Zhu, Qianjin / Chang, Kaixin / Qi, Liyan / Li, Xinyi / Gao, Woming / Gao, Qinwei

    Polymers

    2021  Volume 13, Issue 11

    Abstract: Poly(l-lactic acid) with high molecular weight was used to prepare PLLA films by means of the solvent casting technique. Poly(d-lactic acid) (PDLA) and poly(d-lactic acid- ...

    Abstract Poly(l-lactic acid) with high molecular weight was used to prepare PLLA films by means of the solvent casting technique. Poly(d-lactic acid) (PDLA) and poly(d-lactic acid-
    Language English
    Publishing date 2021-05-27
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2527146-5
    ISSN 2073-4360 ; 2073-4360
    ISSN (online) 2073-4360
    ISSN 2073-4360
    DOI 10.3390/polym13111757
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Lactobacillus plantarum ZY08 relieves chronic alcohol-induced hepatic steatosis and liver injury in mice via restoring intestinal flora homeostasis.

    Ding, Qinchao / Cao, Feiwei / Lai, Shanglei / Zhuge, Hui / Chang, Kaixin / Valencak, Teresa G / Liu, Jianxin / Li, Songtao / Ren, Daxi

    Food research international (Ottawa, Ont.)

    2022  Volume 157, Page(s) 111259

    Abstract: This present study was designed to test the protective role of two Lactobacillus plantarum strains, E680 and ZY08, against alcoholic liver disease (ALD) in C57BL/6 mice. The ALD mouse model was established by exposing the mice to a Lieber-DeCarli ethanol ...

    Abstract This present study was designed to test the protective role of two Lactobacillus plantarum strains, E680 and ZY08, against alcoholic liver disease (ALD) in C57BL/6 mice. The ALD mouse model was established by exposing the mice to a Lieber-DeCarli ethanol liquid diet. The two probiotic strains (10
    MeSH term(s) Animals ; Fatty Liver, Alcoholic/prevention & control ; Gastrointestinal Microbiome ; Homeostasis ; Lactobacillus plantarum ; Liver Diseases, Alcoholic/prevention & control ; Mice ; Mice, Inbred C57BL
    Language English
    Publishing date 2022-04-16
    Publishing country Canada
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1111695-x
    ISSN 1873-7145 ; 0963-9969
    ISSN (online) 1873-7145
    ISSN 0963-9969
    DOI 10.1016/j.foodres.2022.111259
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Lactobacillus plantarum ZY08 relieves chronic alcohol-induced hepatic steatosis and liver injury in mice via restoring intestinal flora homeostasis

    Ding, Qinchao / Cao, Feiwei / Lai, Shanglei / Zhuge, Hui / Chang, Kaixin / Valencak, Teresa G. / Liu, Jianxin / Li, Songtao / Ren, Daxi

    Food Research International. 2022 July, v. 157 p.111259-

    2022  

    Abstract: This present study was designed to test the protective role of two Lactobacillus plantarum strains, E680 and ZY08, against alcoholic liver disease (ALD) in C57BL/6 mice. The ALD mouse model was established by exposing the mice to a Lieber-DeCarli ethanol ...

    Abstract This present study was designed to test the protective role of two Lactobacillus plantarum strains, E680 and ZY08, against alcoholic liver disease (ALD) in C57BL/6 mice. The ALD mouse model was established by exposing the mice to a Lieber-DeCarli ethanol liquid diet. The two probiotic strains (10⁹ cfu/day) were administered by oral gavage, respectively. Our data showed that L. plantarum ZY08, but not E680, intervention significantly mitigated alcohol-related hepatic steatosis, liver injury, intestinal barrier, and it alleviated plasma endotoxin (LPS) levels, and affected hepatic genes relating to lipid metabolism. Furthermore, Lactobacillus plantarum ZY08 effectively restored intestinal flora homeostasis via recovering flora abundance, including Blautia, Oscillibacter, Lachnoclostridium and Intestimonas, and consequently elevated intestinalshort-chain fatty acid (SCFA) content. More importantly, removing intestinal microorganisms through ABX gavage markedly abolished the beneficial aspects of Lactobacillus plantarum ZY08, indicating that the regulative role of Lactobacillus plantarum ZY08 contributed to its protective role against ALD. Overall, Lactobacillus plantarum ZY08 is a potential candidate for mitigating alcohol-induced hepatic steatosis and liver injury.
    Keywords Lactobacillus plantarum ; endotoxins ; ethanol ; fatty acids ; fatty liver ; flora ; food research ; homeostasis ; intestinal microorganisms ; intestines ; lipid metabolism ; liquid diet ; liver ; mice ; models ; probiotics ; protective effect ; Lactobacillus plantarum ZY08 ; Alcoholic liver disease ; Intestinal flora homeostasis ; Intestinal barrier function ; Hepatic steatosis ; Liver injury
    Language English
    Dates of publication 2022-07
    Publishing place Elsevier Ltd
    Document type Article ; Online
    ZDB-ID 1111695-x
    ISSN 1873-7145 ; 0963-9969
    ISSN (online) 1873-7145
    ISSN 0963-9969
    DOI 10.1016/j.foodres.2022.111259
    Database NAL-Catalogue (AGRICOLA)

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  9. Article: Lactobacillus plantarum

    Cao, Feiwei / Ding, Qinchao / Zhuge, Hui / Lai, Shanglei / Chang, Kaixin / Le, Chunyan / Yang, Guorong / Valencak, Teresa G / Li, Songtao / Ren, Daxi

    Frontiers in nutrition

    2023  Volume 9, Page(s) 1071284

    Abstract: This present study was designed to explore the protective role ... ...

    Abstract This present study was designed to explore the protective role of
    Language English
    Publishing date 2023-01-09
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2776676-7
    ISSN 2296-861X
    ISSN 2296-861X
    DOI 10.3389/fnut.2022.1071284
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Xie Zhuo Tiao Zhi formula modulates intestinal microbiota and liver purine metabolism to suppress hepatic steatosis and pyroptosis in NAFLD therapy.

    Qiu, Jiannan / Chen, Lin / Zhang, Ling / Xu, Fangying / Zhang, Congcong / Ren, Guilin / Chang, Kaixin / He, Guonong / Du, Zhongyan / Le, Yifei / Yu, Zhiling / Li, Songtao / Liu, Qingsheng / Dou, Xiaobing

    Phytomedicine : international journal of phytotherapy and phytopharmacology

    2023  Volume 121, Page(s) 155111

    Abstract: Background: Current evidence indicates a rising global prevalence of Non-Alcoholic Fatty Liver Disease (NAFLD), which is closely associated to conditions such as obesity, dyslipidemia, insulin resistance, and metabolic syndrome. The relationship between ...

    Abstract Background: Current evidence indicates a rising global prevalence of Non-Alcoholic Fatty Liver Disease (NAFLD), which is closely associated to conditions such as obesity, dyslipidemia, insulin resistance, and metabolic syndrome. The relationship between the gut microbiome and metabolites in NAFLD is gaining attention understanding the pathogenesis and progression of dysregulated lipid metabolism and inflammation. The Xie Zhuo Tiao Zhi (XZTZ) decoction has been employed in clinical practice for alleviating hyperlipidemia and symptoms related to metabolic disorders. However, the pharmacological mechanisms underlying the effects of XZTZ remain to be elucidated.
    Purpose: The objective of this study was to examine the pharmacological mechanisms underlying the hypolipidemic and anti-inflammatory effects of XZTZ decoction in a mouse model of NAFLD, as well as the effects of supplementing exogenous metabolites on PO induced cell damage and lipid accumulation in cultured hepatocytes.
    Methods: A high-fat diet (HFD) mouse model was established to examine the effects of XZTZ through oral gavage. The general condition of mice and the protective effect of XZTZ on liver injury were evaluated using histological and biochemical methods. Hematoxylin and eosin staining (H&E) staining and oil red O staining were performed to assess inflammatory and lipid accumulation detection, and cytokine levels were quantitatively analyzed. Additionally, the study included full-length 16S rRNA sequencing, liver transcriptome analysis, and non-targeted metabolomics analysis to investigate the relationship among intestinal microbiome, liver metabolic function, and XZTZ decoction.
    Results: XZTZ had a significant impact on the microbial community structure in NAFLD mice. Notably, the abundance of Ileibacterium valens, which was significantly enriched by XZTZ, exhibited a negative correlation with liver injury biomarkers such as, alanine transaminase (ALT) and aspartate transaminase (AST) activity. Moreover, treatment with XZTZ led to a significant enrichment of the purine metabolism pathway in liver tissue metabolites, with inosine, a purine metabolite, showing a significant positive correlation with the abundance of I. valens. XZTZ and inosine also significantly enhanced fatty acid β-oxidation, which led to a reduction in the expression of pro-inflammatory cytokines and the inhibition of liver pyroptosis. These effects contributed to the mitigation of liver injury and hepatocyte damage, both in vivo and vitro. Furthermore, the utilization of HPLC fingerprints and UPLC-Q-TOF-MS elucidated the principal constituents within the XZTZ decoction, including naringin, neohesperidin, atractylenolide III, 23-o-Acetylalisol B, pachymic acid, and ursolic acid which are likely responsible for its therapeutic efficacy. Further investigations are imperative to fully uncover and validate the pharmacodynamic mechanisms underlying these observations.
    Conclusion: The administration of XZTZ decoction demonstrates a protective effect on the livers of NAFLD mice by inhibiting lipid accumulation and reducing hepatocyte inflammatory damage. This protective effect is mediated by the upregulation of I.valens abundance in the intestine, highlighting the importance of the gut-liver axis. Furthermore, the presesnce of inosine, adenosine, and their derivatives are important in promoting the protective effects of XZTZ. Furthermore, the in vitro approaching, we provide hitherto undocumented evidence indicating that the inosine significantly improves lipid accumulation, inflammatory damage, and pyroptosis in AML12 cells incubated with free fatty acids.
    MeSH term(s) Animals ; Mice ; Non-alcoholic Fatty Liver Disease/metabolism ; Gastrointestinal Microbiome ; Pyroptosis ; RNA, Ribosomal, 16S ; Liver ; Lipid Metabolism ; Diet, High-Fat/adverse effects ; Fatty Acids, Nonesterified/metabolism ; Purines/pharmacology ; Inosine/metabolism ; Inosine/pharmacology ; Inosine/therapeutic use ; Mice, Inbred C57BL
    Chemical Substances RNA, Ribosomal, 16S ; Fatty Acids, Nonesterified ; Purines ; Inosine (5A614L51CT)
    Language English
    Publishing date 2023-09-23
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 1205240-1
    ISSN 1618-095X ; 0944-7113
    ISSN (online) 1618-095X
    ISSN 0944-7113
    DOI 10.1016/j.phymed.2023.155111
    Database MEDical Literature Analysis and Retrieval System OnLINE

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