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  1. Article: Multiscale label-free imaging of myelin in human brain tissue with polarization-sensitive optical coherence tomography and birefringence microscopy.

    Blanke, Nathan / Chang, Shuaibin / Novoseltseva, Anna / Wang, Hui / Boas, David A / Bigio, Irving J

    Biomedical optics express

    2023  Volume 14, Issue 11, Page(s) 5946–5964

    Abstract: The combination of polarization-sensitive optical coherence tomography (PS-OCT) and birefringence microscopy (BRM) enables multiscale assessment of myelinated axons in postmortem brain tissue, and these tools are promising for the study of brain ... ...

    Abstract The combination of polarization-sensitive optical coherence tomography (PS-OCT) and birefringence microscopy (BRM) enables multiscale assessment of myelinated axons in postmortem brain tissue, and these tools are promising for the study of brain connectivity and organization. We demonstrate label-free imaging of myelin structure across the mesoscopic and microscopic spatial scales by performing serial-sectioning PS-OCT of a block of human brain tissue and periodically sampling thin sections for high-resolution imaging with BRM. In co-registered birefringence parameter maps, we observe good correspondence and demonstrate that BRM enables detailed validation of myelin (hence, axonal) organization, thus complementing the volumetric information content of PS-OCT.
    Language English
    Publishing date 2023-10-24
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2572216-5
    ISSN 2156-7085
    ISSN 2156-7085
    DOI 10.1364/BOE.499354
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Enhanced Multiscale Human Brain Imaging by Semi-supervised Digital Staining and Serial Sectioning Optical Coherence Tomography.

    Cheng, Shiyi / Chang, Shuaibin / Li, Yunzhe / Novoseltseva, Anna / Lin, Sunni / Wu, Yicun / Zhu, Jiahui / McKee, Ann C / Rosene, Douglas L / Wang, Hui / Bigio, Irving J / Boas, David A / Tian, Lei

    Research square

    2024  

    Abstract: A major challenge in neuroscience is to visualize the structure of the human brain at different scales. Traditional histology reveals micro- and meso-scale brain features, but suffers from staining variability, tissue damage and distortion that impedes ... ...

    Abstract A major challenge in neuroscience is to visualize the structure of the human brain at different scales. Traditional histology reveals micro- and meso-scale brain features, but suffers from staining variability, tissue damage and distortion that impedes accurate 3D reconstructions. Here, we present a new 3D imaging framework that combines serial sectioning optical coherence tomography (S-OCT) with a deep-learning digital staining (DS) model. We develop a novel semi-supervised learning technique to facilitate DS model training on weakly paired images. The DS model performs translation from S-OCT to Gallyas silver staining. We demonstrate DS on various human cerebral cortex samples with consistent staining quality. Additionally, we show that DS enhances contrast across cortical layer boundaries. Furthermore, we showcase geometry-preserving 3D DS on cubic-centimeter tissue blocks and visualization of meso-scale vessel networks in the white matter. We believe that our technique offers the potential for high-throughput, multiscale imaging of brain tissues and may facilitate studies of brain structures.
    Language English
    Publishing date 2024-03-21
    Publishing country United States
    Document type Preprint
    DOI 10.21203/rs.3.rs-4014687/v1
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Multi-Scale Label-free Human Brain Imaging with Integrated Serial Sectioning Polarization Sensitive Optical Coherence Tomography and Two-Photon Microscopy.

    Chang, Shuaibin / Yang, Jiarui / Novoseltseva, Anna / Fu, Xinlei / Li, Chenglin / Chen, Shih-Chi / Augustinack, Jean C / Magnain, Caroline / Fischl, Bruce / Mckee, Ann C / Boas, David A / Chen, Ichun Anderson / Wang, Hui

    bioRxiv : the preprint server for biology

    2023  

    Abstract: The study of neurodegenerative processes in the human brain requires a comprehensive understanding of cytoarchitectonic, myeloarchitectonic, and vascular structures. Recent computational advances have enabled volumetric reconstruction of the human brain ... ...

    Abstract The study of neurodegenerative processes in the human brain requires a comprehensive understanding of cytoarchitectonic, myeloarchitectonic, and vascular structures. Recent computational advances have enabled volumetric reconstruction of the human brain using thousands of stained slices, however, tissue distortions and loss resulting from standard histological processing have hindered deformation-free reconstruction of the human brain. The development of a multi-scale and volumetric human brain imaging technique that can measure intact brain structure would be a major technical advance. Here, we describe the development of integrated serial sectioning Polarization Sensitive Optical Coherence Tomography (PSOCT) and Two Photon Microscopy (2PM) to provide label-free multi-contrast imaging, including scattering, birefringence and autofluorescence of human brain tissue. We demonstrate that high-throughput reconstruction of 4×4×2cm
    Language English
    Publishing date 2023-05-24
    Publishing country United States
    Document type Preprint
    DOI 10.1101/2023.05.22.541785
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Multi-Scale Label-Free Human Brain Imaging with Integrated Serial Sectioning Polarization Sensitive Optical Coherence Tomography and Two-Photon Microscopy.

    Chang, Shuaibin / Yang, Jiarui / Novoseltseva, Anna / Abdelhakeem, Ayman / Hyman, Mackenzie / Fu, Xinlei / Li, Chenglin / Chen, Shih-Chi / Augustinack, Jean C / Magnain, Caroline / Fischl, Bruce / Mckee, Ann C / Boas, David A / Chen, Ichun Anderson / Wang, Hui

    Advanced science (Weinheim, Baden-Wurttemberg, Germany)

    2023  Volume 10, Issue 35, Page(s) e2303381

    Abstract: The study of aging and neurodegenerative processes in the human brain requires a comprehensive understanding of cytoarchitectonic, myeloarchitectonic, and vascular structures. Recent computational advances have enabled volumetric reconstruction of the ... ...

    Abstract The study of aging and neurodegenerative processes in the human brain requires a comprehensive understanding of cytoarchitectonic, myeloarchitectonic, and vascular structures. Recent computational advances have enabled volumetric reconstruction of the human brain using thousands of stained slices, however, tissue distortions and loss resulting from standard histological processing have hindered deformation-free reconstruction. Here, the authors describe an integrated serial sectioning polarization-sensitive optical coherence tomography (PSOCT) and two photon microscopy (2PM) system to provide label-free multi-contrast imaging of intact brain structures, including scattering, birefringence, and autofluorescence of human brain tissue. The authors demonstrate high-throughput reconstruction of 4 × 4 × 2cm
    MeSH term(s) Humans ; Tomography, Optical Coherence/methods ; Microscopy/methods ; Lipofuscin ; Brain/diagnostic imaging ; Neuroimaging
    Chemical Substances Lipofuscin
    Language English
    Publishing date 2023-10-26
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2808093-2
    ISSN 2198-3844 ; 2198-3844
    ISSN (online) 2198-3844
    ISSN 2198-3844
    DOI 10.1002/advs.202303381
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Scalable mapping of myelin and neuron density in the human brain with micrometer resolution.

    Chang, Shuaibin / Varadarajan, Divya / Yang, Jiarui / Chen, Ichun Anderson / Kura, Sreekanth / Magnain, Caroline / Augustinack, Jean C / Fischl, Bruce / Greve, Douglas N / Boas, David A / Wang, Hui

    Scientific reports

    2022  Volume 12, Issue 1, Page(s) 363

    Abstract: Optical coherence tomography (OCT) is an emerging 3D imaging technique that allows quantification of intrinsic optical properties such as scattering coefficient and back-scattering coefficient, and has proved useful in distinguishing delicate ... ...

    Abstract Optical coherence tomography (OCT) is an emerging 3D imaging technique that allows quantification of intrinsic optical properties such as scattering coefficient and back-scattering coefficient, and has proved useful in distinguishing delicate microstructures in the human brain. The origins of scattering in brain tissues are contributed by the myelin content, neuron size and density primarily; however, no quantitative relationships between them have been reported, which hampers the use of OCT in fundamental studies of architectonic areas in the human brain and the pathological evaluations of diseases. Here, we built a generalized linear model based on Mie scattering theory that quantitatively links tissue scattering to myelin content and neuron density in the human brain. We report a strong linear relationship between scattering coefficient and the myelin content that is retained across different regions of the brain. Neuronal cell body turns out to be a secondary contribution to the overall scattering. The optical property of OCT provides a label-free solution for quantifying volumetric myelin content and neuron cells in the human brain.
    MeSH term(s) Adult ; Aged ; Brain/cytology ; Brain/diagnostic imaging ; Brain/metabolism ; Cadaver ; Female ; Humans ; Imaging, Three-Dimensional ; Lasers ; Male ; Middle Aged ; Myelin Sheath ; Neuroimaging ; Neurons/chemistry ; Predictive Value of Tests ; Reproducibility of Results ; Scattering, Radiation ; Tomography, Optical Coherence
    Language English
    Publishing date 2022-01-10
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-021-04093-y
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: Refractive-index matching enhanced polarization sensitive optical coherence tomography quantification in human brain tissue.

    Liu, Chao J / Ammon, William / Jones, Robert J / Nolan, Jackson / Wang, Ruopeng / Chang, Shuaibin / Frosch, Matthew P / Yendiki, Anastasia / Boas, David A / Magnain, Caroline / Fischl, Bruce / Wang, Hui

    Biomedical optics express

    2021  Volume 13, Issue 1, Page(s) 358–372

    Abstract: The importance of polarization-sensitive optical coherence tomography (PS-OCT) has been increasingly recognized in human brain imaging. Despite the recent progress of PS-OCT in revealing white matter architecture and orientation, quantification of fine- ... ...

    Abstract The importance of polarization-sensitive optical coherence tomography (PS-OCT) has been increasingly recognized in human brain imaging. Despite the recent progress of PS-OCT in revealing white matter architecture and orientation, quantification of fine-scale fiber tracts in the human brain cortex has been a challenging problem, due to a low birefringence in the gray matter. In this study, we investigated the effect of refractive index matching by 2,2'-thiodiethanol (TDE) immersion on the improvement of PS-OCT measurements in
    Language English
    Publishing date 2021-12-15
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2572216-5
    ISSN 2156-7085
    ISSN 2156-7085
    DOI 10.1364/BOE.443066
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Volumetric Characterization of Microvasculature in Ex Vivo Human Brain Samples By Serial Sectioning Optical Coherence Tomography.

    Yang, Jiarui / Chang, Shuaibin / Chen, Ichun Anderson / Kura, Sreekanth / Rosen, Grace A / Saltiel, Nicole A / Huber, Bertrand R / Varadarajan, Divya / Balbastre, Yael / Magnain, Caroline / Chen, Shih-Chi / Fischl, Bruce / McKee, Ann C / Boas, David A / Wang, Hui

    IEEE transactions on bio-medical engineering

    2022  Volume 69, Issue 12, Page(s) 3645–3656

    Abstract: Objective: Serial sectioning optical coherence tomography (OCT) enables accurate volumetric reconstruction of several cubic centimeters of human brain samples. We aimed to identify anatomical features of the ex vivo human brain, such as intraparenchymal ...

    Abstract Objective: Serial sectioning optical coherence tomography (OCT) enables accurate volumetric reconstruction of several cubic centimeters of human brain samples. We aimed to identify anatomical features of the ex vivo human brain, such as intraparenchymal blood vessels and axonal fiber bundles, from the OCT data in 3D, using intrinsic optical contrast.
    Methods: We developed an automatic processing pipeline to enable characterization of the intraparenchymal microvascular network in human brain samples.
    Results: We demonstrated the automatic extraction of the vessels down to a 20 μm in diameter using a filtering strategy followed by a graphing representation and characterization of the geometrical properties of microvascular network in 3D. We also showed the ability to extend this processing strategy to extract axonal fiber bundles from the volumetric OCT image.
    Conclusion: This method provides a viable tool for quantitative characterization of volumetric microvascular network as well as the axonal bundle properties in normal and pathological tissues of the ex vivo human brain.
    MeSH term(s) Humans ; Tomography, Optical Coherence/methods ; Imaging, Three-Dimensional/methods ; Brain/diagnostic imaging ; Microvessels/diagnostic imaging ; Histological Techniques
    Language English
    Publishing date 2022-11-23
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, N.I.H., Extramural
    ZDB-ID 160429-6
    ISSN 1558-2531 ; 0018-9294
    ISSN (online) 1558-2531
    ISSN 0018-9294
    DOI 10.1109/TBME.2022.3175072
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

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  8. Article: Improving the characterization of

    Yang, Jiarui / Chen, Ichun Anderson / Chang, Shuaibin / Tang, Jianbo / Lee, Blaire / Kılıç, Kıvılcım / Sunil, Smrithi / Wang, Hui / Varadarajan, Divya / Magnain, Caroline / Chen, Shih-Chi / Costantini, Irene / Pavone, Francesco / Fischl, Bruce / Boas, David A

    Neurophotonics

    2020  Volume 7, Issue 4, Page(s) 45005

    Abstract: Significance: ...

    Abstract Significance:
    Language English
    Publishing date 2020-10-21
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2781943-7
    ISSN 2329-4248 ; 2329-423X
    ISSN (online) 2329-4248
    ISSN 2329-423X
    DOI 10.1117/1.NPh.7.4.045005
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  9. Article ; Online: A cellular resolution atlas of Broca's area.

    Costantini, Irene / Morgan, Leah / Yang, Jiarui / Balbastre, Yael / Varadarajan, Divya / Pesce, Luca / Scardigli, Marina / Mazzamuto, Giacomo / Gavryusev, Vladislav / Castelli, Filippo Maria / Roffilli, Matteo / Silvestri, Ludovico / Laffey, Jessie / Raia, Sophia / Varghese, Merina / Wicinski, Bridget / Chang, Shuaibin / Chen, Ichun Anderson / Wang, Hui /
    Cordero, Devani / Vera, Matthew / Nolan, Jackson / Nestor, Kimberly / Mora, Jocelyn / Iglesias, Juan Eugenio / Garcia Pallares, Erendira / Evancic, Kathryn / Augustinack, Jean C / Fogarty, Morgan / Dalca, Adrian V / Frosch, Matthew P / Magnain, Caroline / Frost, Robert / van der Kouwe, Andre / Chen, Shih-Chi / Boas, David A / Pavone, Francesco Saverio / Fischl, Bruce / Hof, Patrick R

    Science advances

    2023  Volume 9, Issue 41, Page(s) eadg3844

    Abstract: Brain cells are arranged in laminar, nuclear, or columnar structures, spanning a range of scales. Here, we construct a reliable cell census in the frontal lobe of human cerebral cortex at micrometer resolution in a magnetic resonance imaging (MRI)- ... ...

    Abstract Brain cells are arranged in laminar, nuclear, or columnar structures, spanning a range of scales. Here, we construct a reliable cell census in the frontal lobe of human cerebral cortex at micrometer resolution in a magnetic resonance imaging (MRI)-referenced system using innovative imaging and analysis methodologies. MRI establishes a macroscopic reference coordinate system of laminar and cytoarchitectural boundaries. Cell counting is obtained with a digital stereological approach on the 3D reconstruction at cellular resolution from a custom-made inverted confocal light-sheet fluorescence microscope (LSFM). Mesoscale optical coherence tomography enables the registration of the distorted histological cell typing obtained with LSFM to the MRI-based atlas coordinate system. The outcome is an integrated high-resolution cellular census of Broca's area in a human postmortem specimen, within a whole-brain reference space atlas.
    MeSH term(s) Humans ; Broca Area ; Cerebral Cortex ; Brain/diagnostic imaging ; Magnetic Resonance Imaging/methods ; Brain Mapping
    Language English
    Publishing date 2023-10-12
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2810933-8
    ISSN 2375-2548 ; 2375-2548
    ISSN (online) 2375-2548
    ISSN 2375-2548
    DOI 10.1126/sciadv.adg3844
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