Article ; Online: Long noncoding RNA MEG3 regulates cell proliferation and apoptosis by disrupting microRNA-9-5p-mediated inhibition of NDRG1 in prostate cancer.
Aging
2024 Volume 16, Issue 2, Page(s) 1938–1951
Abstract: Background: Long noncoding RNA MEG3 has been described to be involved in the regulation of gene expression and cancer progression. However, the role of lncMEG3 in prostate cancer (PCa) remains largely uncharted.: Methods: Differential expression of ... ...
Abstract | Background: Long noncoding RNA MEG3 has been described to be involved in the regulation of gene expression and cancer progression. However, the role of lncMEG3 in prostate cancer (PCa) remains largely uncharted. Methods: Differential expression of lncMEG3 was identified in PCa tissues using RNA-sequencing analysis. qRT-PCR was performed to examine the level of lncMEG3. Additionally, cellular fractionation and fluorescent Results: We observed downregulation of lncMEG3 in PCa cells and tissues. Patients with lower levels of lncMEG3 had a higher likelihood of experiencing biochemical recurrence. Overexpression of lncMEG3 resulted in the inhibition of PCa cell proliferation and the promotion of apoptosis. Moreover, lncMEG3 is competitively bound to miR-9-5p, preventing its inhibitory effect on the target gene NDRG1. This ultimately led to the inhibition of PCa cell proliferation and the promotion of apoptosis. Furthermore, increasing lncMEG3 levels also demonstrated inhibitory effects on PCa proliferation and promotion of apoptosis Conclusions: Our findings uncover a crucial role for lncMEG3 in inhibiting PCa proliferation and promoting apoptosis through disruption of miR-9-5p-mediated inhibition of NDRG1. |
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MeSH term(s) | Humans ; Male ; Apoptosis/genetics ; Cell Line, Tumor ; Cell Proliferation/genetics ; Gene Expression Regulation, Neoplastic ; In Situ Hybridization, Fluorescence ; MicroRNAs/metabolism ; Prostatic Neoplasms/genetics ; Prostatic Neoplasms/metabolism ; RNA, Long Noncoding/genetics ; RNA, Long Noncoding/pharmacology |
Chemical Substances | MicroRNAs ; RNA, Long Noncoding ; N-myc downstream-regulated gene 1 protein ; MIRN9 microRNA, human ; MEG3 non-coding RNA, human |
Language | English |
Publishing date | 2024-01-24 |
Publishing country | United States |
Document type | Journal Article |
ISSN | 1945-4589 |
ISSN (online) | 1945-4589 |
DOI | 10.18632/aging.205472 |
Database | MEDical Literature Analysis and Retrieval System OnLINE |
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