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Article ; Online: Une hypopigmentation cutanée.

Nabitz, C / Chantalat, L / Fourgeaud, C / Vignes, S

2020 Volume 42, Issue 7, Page(s) 513–514

Title translation	A skin hypopigmentation.
MeSH term(s)	Atrophy/pathology ; Glucocorticoids ; Humans ; Hypopigmentation/diagnosis ; Hypopigmentation/etiology ; Hypopigmentation/pathology ; Skin/pathology
Chemical Substances	Glucocorticoids
Language	French
Publishing date	2020-12-03
Publishing country	France
Document type	Journal Article
ZDB-ID	604679-4
ISSN	1768-3122 ; 0248-8663
ISSN (online)	1768-3122
ISSN	0248-8663
DOI	10.1016/j.revmed.2020.11.004
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Article ; Online: Le syndrome des grosses mains : un diagnostic méconnu.

Chantalat, L / Simon, M / Fourgeaud, C / Vignes, S

La Revue de medecine interne

2020 Volume 41, Issue 10, Page(s) 700–703

Abstract: Introduction: Puffy hand syndrome is a rare complication of intravenous drug addiction. Diagnosis is based on the patient's history and clinical examination.: Observations: A woman and two men, aged 42, 39 and 36 years old, are described. All had a ... ...

Title translation	Puffy hand syndrome: A little-known diagnosis.
Abstract	Introduction: Puffy hand syndrome is a rare complication of intravenous drug addiction. Diagnosis is based on the patient's history and clinical examination. Observations: A woman and two men, aged 42, 39 and 36 years old, are described. All had a history of intravenous drug use of heroin and oral buprenorphine misuse. Puffy hand syndrome appeared during drug addiction (n = 2) or after its withdrawal (n = 1). It was associated with acrocyanosis (n = 1) or injection scars (n = 1). Upper limb ultrasonography showed sequelae of venous (n = 3) or arterial (n = 1) thrombosis. An upper limb lymphoscintigraphy in one patient showed decreased radionuclide uptake of axillary lymph node and subdermal reflux tracer in the forearm. Treatment was based on low-stretch bandages to reduce the volume and then elastic compression sleeve for long-term stabilization. Conclusion: Puffy hand syndrome seen in intravenous drug addicts is poorly understood. It is a chronic complication despite the cessation of drug use. This syndrome has to become more widely known because its management is mandatory, although symptomatic.
MeSH term(s)	Adult ; Buprenorphine/therapeutic use ; Diagnosis, Differential ; Female ; Hand/pathology ; Heroin Dependence/complications ; Heroin Dependence/drug therapy ; Heroin Dependence/pathology ; Humans ; Lymphedema/diagnosis ; Lymphedema/etiology ; Lymphedema/pathology ; Male ; Opiate Substitution Treatment ; Substance Abuse, Intravenous/complications ; Substance Abuse, Intravenous/drug therapy ; Substance Abuse, Intravenous/pathology ; Syndrome
Chemical Substances	Buprenorphine (40D3SCR4GZ)
Language	French
Publishing date	2020-07-14
Publishing country	France
Document type	Case Reports ; Journal Article
ZDB-ID	604679-4
ISSN	1768-3122 ; 0248-8663
ISSN (online)	1768-3122
ISSN	0248-8663
DOI	10.1016/j.revmed.2020.05.020
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Article ; Online: Des veines anormales.

Chantalat, L / Fourgeaud, C / Simon, M / Vignes, S

La Revue de medecine interne

2019 Volume 41, Issue 7, Page(s) 502–503

Title translation	Abnormal veins.
MeSH term(s)	Aged, 80 and over ; Cardiovascular Abnormalities/diagnosis ; Cardiovascular Abnormalities/pathology ; Collateral Circulation/physiology ; Female ; Humans ; Shoulder/blood supply ; Shoulder/pathology ; Veins/abnormalities ; Veins/pathology
Language	French
Publishing date	2019-12-26
Publishing country	France
Document type	Case Reports ; Journal Article
ZDB-ID	604679-4
ISSN	1768-3122 ; 0248-8663
ISSN (online)	1768-3122
ISSN	0248-8663
DOI	10.1016/j.revmed.2019.12.010
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Article ; Online: [No title information]

Martiano, M / Chantalat, L / Simon, M / Fourgeaud, C / Vignes, S

La Revue de medecine interne

2020 Volume 41, Issue 9, Page(s) 637–638

Title translation	Des vésicules cutanées.
MeSH term(s)	Adult ; Antineoplastic Combined Chemotherapy Protocols/therapeutic use ; Breast Neoplasms/radiotherapy ; Breast Neoplasms/therapy ; Carcinoma, Ductal, Breast/radiotherapy ; Carcinoma, Ductal, Breast/therapy ; Combined Modality Therapy/adverse effects ; Diagnosis, Differential ; Female ; Humans ; Lymphangiectasis/diagnosis ; Lymphangiectasis/etiology ; Mastectomy ; Radiation Injuries/diagnosis ; Radiotherapy, Conformal/adverse effects ; Skin Diseases, Vesiculobullous/diagnosis ; Skin Diseases, Vesiculobullous/etiology ; Tamoxifen/administration & dosage
Chemical Substances	Tamoxifen (094ZI81Y45)
Language	French
Publishing date	2020-04-28
Publishing country	France
Document type	Case Reports ; Journal Article
ZDB-ID	604679-4
ISSN	1768-3122 ; 0248-8663
ISSN (online)	1768-3122
ISSN	0248-8663
DOI	10.1016/j.revmed.2020.03.007
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Article: Structural studies of streptococcus agalactiae hyaluronate lyase.

Jedrzejas, M J / Chantalat, L

Acta crystallographica. Section D, Biological crystallography

2000 Volume 56, Issue Pt 4, Page(s) 460–463

Abstract: The bacteria Streptococcus agalactiae, part of normal human flora, produce an enzyme, hyaluronate lyase, which appears to contribute to the invasive capacity of this pathogen by degrading hyaluronan and chondroitin sulfates of the extracellular matrix of ...

Abstract	The bacteria Streptococcus agalactiae, part of normal human flora, produce an enzyme, hyaluronate lyase, which appears to contribute to the invasive capacity of this pathogen by degrading hyaluronan and chondroitin sulfates of the extracellular matrix of host tissues. The native enzyme, the product of the hylB(3502) allele, has a molecular mass of 111 kDa but undergoes an autocatalytic conversion to a smaller enzymatically active 92 kDa form. To initiate the determination of the catalytic mechanism of action of these enzymes, the 111 and 92 kDa enzymes were crystallized by a vapor-diffusion method using polyethylene glycol monomethyl ether 5000 and potassium thiocyanate as precipitating agents. The 111 kDa enzyme crystals are of poor quality and diffract X-rays to a very low resolution. However, the crystals of the truncated 92 kDa enzyme diffract X-rays to 2.1 A resolution. The crystal symmetry is C222(1) and the unit-cell parameters are a = 51.69, b = 157.03, c = 239.20 A (alpha = beta = gamma = 90 degrees ). The V(m) of 2.64 A(3) Da(-1) is consistent with the presence of one molecule of hyaluronate lyase in the asymmetric unit and a crystal solvent content of 53%. An isomorphous ethylmercuricthiosalicylic acid heavy-atom derivative diffraction data set has been obtained in order to solve the structure.
MeSH term(s)	Catalysis ; Crystallography, X-Ray/methods ; Humans ; Kinetics ; Molecular Weight ; Polysaccharide-Lyases/chemistry ; Polysaccharide-Lyases/isolation & purification ; Polysaccharide-Lyases/metabolism ; Recombinant Proteins/chemistry ; Recombinant Proteins/isolation & purification ; Recombinant Proteins/metabolism ; Streptococcus agalactiae/enzymology
Chemical Substances	Recombinant Proteins ; Polysaccharide-Lyases (EC 4.2.2.-) ; hyaluronate lyase (EC 4.2.2.1)
Language	English
Publishing date	2000-04
Publishing country	United States
Document type	Journal Article ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, U.S. Gov't, P.H.S.
ZDB-ID	2020492-9
ISSN	2059-7983 ; 1399-0047 ; 0907-4449
ISSN (online)	2059-7983 ; 1399-0047
ISSN	0907-4449
DOI	10.1107/s0907444900000706
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Article ; Online: Measurement of Nocturnal Scratching in Patients with Pruritus Using a Smartwatch: Initial Clinical Studies with the Itch Tracker App.

Ikoma, Akihiko / Ebata, Toshiya / Chantalat, Laurent / Takemura, Kimitoshi / Mizzi, Fabienne / Poncet, Michel / LeClercq, Didier

Acta dermato-venereologica

2018 Volume 99, Issue 3, Page(s) 268–273

Abstract: Three clinical studies were conducted to test a newly-developed app for smartwatches, which included an algorithm to measure nocturnal scratching using acceleration data. The first study in 5 patients with atopic dermatitis demonstrated high reliability ... ...

Abstract	Three clinical studies were conducted to test a newly-developed app for smartwatches, which included an algorithm to measure nocturnal scratching using acceleration data. The first study in 5 patients with atopic dermatitis demonstrated high reliability of the app for measurement of scratching compared with video monitoring (positive predictive value 90.2 ± 6.6%, sensitivity 84.6 ± 10.2%, correlation of scratching duration per h r = 0.851-0.901, p < 0.001). The second study in 20 patients with atopic dermatitis and 10 healthy volunteers showed that total scratching duration in patients was significantly longer than in healthy volunteers and correlated positively with Eczema Area and Severity Index (EASI) scores. In the third study, conducted in an open-entry manner in which 201 evaluable participants measured nocturnal scratching, those who self-reported itch or pruritic diseases had a significantly longer duration of scratching than those who did not. In conclusion, this app has a high reliability and potential clinical usefulness for measurement of nocturnal scratching.
MeSH term(s)	Adult ; Case-Control Studies ; Circadian Rhythm ; Computers, Handheld ; Dermatitis, Atopic/complications ; Dermatitis, Atopic/diagnosis ; Dermatitis, Atopic/physiopathology ; Female ; Humans ; Male ; Middle Aged ; Mobile Applications ; Movement ; Predictive Value of Tests ; Pruritus/diagnosis ; Pruritus/etiology ; Pruritus/physiopathology ; Reproducibility of Results ; Severity of Illness Index ; Sleep ; Time Factors
Language	English
Publishing date	2018-12-07
Publishing country	Sweden
Document type	Clinical Study ; Journal Article
ZDB-ID	80007-7
ISSN	1651-2057 ; 0001-5555
ISSN (online)	1651-2057
ISSN	0001-5555
DOI	10.2340/00015555-3105
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Article: Crystallization and preliminary X-ray analysis of Streptococcus pneumoniae hyaluronate lyase.

Jedrzejas, M J / Chantalat, L / Mewbourne, R B

Journal of structural biology

1998 Volume 121, Issue 1, Page(s) 73–75

Abstract: A fully active 83-kDa truncated form of recombinant hyaluronate lyase from Streptococcus pneumoniae was crystallized by the hanging drop vapor diffusion method using ammonium sulfate as a precipitating agent. Crystals grew at room temperature using a ... ...

Abstract	A fully active 83-kDa truncated form of recombinant hyaluronate lyase from Streptococcus pneumoniae was crystallized by the hanging drop vapor diffusion method using ammonium sulfate as a precipitating agent. Crystals grew at room temperature using a variety of buffers with pH around 6. The crystals diffract X-rays beyond 2.0 A resolution using Cu K alpha radiation and a rotating-anode X-ray source. They belong to the orthorhombic space group P2(1)2(1)2(1) with unit cell dimensions, a = 84.2, b = 104.2, c = 104.6 A, and alpha = beta = gamma = 90.0 degrees. The VM value of 2.9 A3/Da is consistent with only one molecule of the enzyme in the asymmetric unit and the solvent content of 57%. Diffraction data 94.7% complete to 2.0 A resolution with Rsym of 5.4% were collected from one native crystal at room temperature. The search for heavy-atom derivatives to solve the structure is in progress.
MeSH term(s)	Crystallization ; Crystallography, X-Ray ; Escherichia coli/genetics ; Molecular Weight ; Peptide Fragments/chemistry ; Peptide Fragments/genetics ; Peptide Fragments/isolation & purification ; Polysaccharide-Lyases/chemistry ; Polysaccharide-Lyases/genetics ; Polysaccharide-Lyases/isolation & purification ; Recombinant Proteins/chemistry ; Recombinant Proteins/genetics ; Recombinant Proteins/isolation & purification ; Streptococcus pneumoniae/enzymology ; Streptococcus pneumoniae/genetics
Chemical Substances	Peptide Fragments ; Recombinant Proteins ; Polysaccharide-Lyases (EC 4.2.2.-) ; hyaluronate lyase (EC 4.2.2.1)
Language	English
Publishing date	1998-01
Publishing country	United States
Document type	Journal Article
ZDB-ID	1032718-6
ISSN	1095-8657 ; 1047-8477
ISSN (online)	1095-8657
ISSN	1047-8477
DOI	10.1006/jsbi.1998.3963
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Article: On the Operational Aspects of Measuring Nanoparticle Sizes.

Teulon, Jean-Marie / Godon, Christian / Chantalat, Louis / Moriscot, Christine / Cambedouzou, Julien / Odorico, Michael / Ravaux, Johann / Podor, Renaud / Gerdil, Adèle / Habert, Aurélie / Herlin-Boime, Nathalie / Chen, Shu-Wen W / Pellequer, Jean-Luc

Nanomaterials (Basel, Switzerland)

2018 Volume 9, Issue 1

Abstract: Nanoparticles are defined as elementary particles with a size between 1 and 100 nm for at least 50% (in number). They can be made from natural materials, or manufactured. Due to their small sizes, novel toxicological issues are raised and thus ... ...

Abstract	Nanoparticles are defined as elementary particles with a size between 1 and 100 nm for at least 50% (in number). They can be made from natural materials, or manufactured. Due to their small sizes, novel toxicological issues are raised and thus determining the accurate size of these nanoparticles is a major challenge. In this study, we performed an intercomparison experiment with the goal to measure sizes of several nanoparticles, in a first step, calibrated beads and monodispersed SiO₂ Ludox®, and, in a second step, nanoparticles (NPs) of toxicological interest, such as Silver NM-300 K and PVP-coated Ag NPs, Titanium dioxide A12, P25(Degussa), and E171(A), using commonly available laboratory techniques such as transmission electron microscopy, scanning electron microscopy, small-angle X-ray scattering, dynamic light scattering, wet scanning transmission electron microscopy (and its dry state, STEM) and atomic force microscopy. With monomodal distributed NPs (polystyrene beads and SiO₂ Ludox®), all tested techniques provide a global size value amplitude within 25% from each other, whereas on multimodal distributed NPs (Ag and TiO₂) the inter-technique variation in size values reaches 300%. Our results highlight several pitfalls of NP size measurements such as operational aspects, which are unexpected consequences in the choice of experimental protocols. It reinforces the idea that averaging the NP size from different biophysical techniques (and experimental protocols) is more robust than focusing on repetitions of a single technique. Besides, when characterizing a heterogeneous NP in size, a size distribution is more informative than a simple average value. This work emphasizes the need for nanotoxicologists (and regulatory agencies) to test a large panel of different techniques before making a choice for the most appropriate technique(s)/protocol(s) to characterize a peculiar NP.
Language	English
Publishing date	2018-12-23
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2662255-5
ISSN	2079-4991
ISSN	2079-4991
DOI	10.3390/nano9010018
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Article: Exploratory clinical trial to evaluate the efficacy of a topical traditional chinese herbal medicine in psoriasis vulgaris.

Yan, Yuhe / Liu, Wali / Andres, Philippe / Pernin, Colette / Chantalat, Laurent / Briantais, Philippe / Lin, Albert / Feng, Lilian

Evidence-based complementary and alternative medicine : eCAM

2015 Volume 2015, Page(s) 719641

Abstract: Objective. To evaluate the efficacy and safety of herbal ointment, Shi Du Ruan Gao, in patients with plaque-type psoriasis. Design. Single-center, randomized, investigator-blinded, parallel group, placebo-controlled study. Participants. One hundred ... ...

Abstract	Objective. To evaluate the efficacy and safety of herbal ointment, Shi Du Ruan Gao, in patients with plaque-type psoriasis. Design. Single-center, randomized, investigator-blinded, parallel group, placebo-controlled study. Participants. One hundred outpatients with mild to moderate chronic plaque-type psoriasis were enrolled. Intervention. The patients applied either Shi Du Ruan Gao ointment or vehicle ointment topically to for 8 weeks. Main Outcome Measures. The outcomes were assessed using the following criteria: Total Severity Score (TSS, sum of erythema, scaling, and plaque elevation/induration, on a 0 to 4 scale), Investigator Global Assessment (IGA) evaluated on a 0 (Clear) to 4 (s to very severe) scale, and Global Subjects' Assessment of treatment response on a 7-point scale from -1 (worse) to 5 (Cleared). Results. Significant reductions in the Total Severity Score (P < 0.001) (mean score: 2.7 after Shi Du Ruan Gao treatment versus 5.1 in control subjects). Both Investigator Global Assessment (IGA) and Global Subjects' Assessment of treatment are better in the Shi Du Ruan Gao group than the control group (P < 0.001). Conclusion. Shi Du Ruan Gao ointment was a safe, and effective therapy for plaque-type psoriasis.
Language	English
Publishing date	2015-03-05
Publishing country	United States
Document type	Journal Article
ZDB-ID	2171158-6
ISSN	1741-4288 ; 1741-427X
ISSN (online)	1741-4288
ISSN	1741-427X
DOI	10.1155/2015/719641
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Article ; Online: New Caspase-1 inhibitor by scaffold hopping into bio-inspired 3D-fragment space.

Brethon, Anne / Chantalat, Laurent / Christin, Olivier / Clary, Laurence / Fournier, Jean-François / Gastreich, Marcus / Harris, Craig S / Isabet, Tatiana / Pascau, Jonathan / Thoreau, Etienne / Roche, Didier / Rodeschini, Vincent

Bioorganic & medicinal chemistry letters

2017

Abstract: Virtual fragmentation of a library of 12,000 compounds inspired by natural products led to a dataset of 153,000 fragments that was used as a source to identify effective P2-P3 scaffold replacement solutions for peptidic Caspase-1 inhibitors. Our strategy ...

Abstract	Virtual fragmentation of a library of 12,000 compounds inspired by natural products led to a dataset of 153,000 fragments that was used as a source to identify effective P2-P3 scaffold replacement solutions for peptidic Caspase-1 inhibitors. Our strategy led to the identification of an original 2-azabicyclo-octane scaffold (2-ABO) that was further elaborated into the potent Caspase-1 inhibitor CD10847 (IC
Language	English
Publishing date	2017-11-09
Publishing country	England
Document type	Journal Article
ZDB-ID	1063195-1
ISSN	1464-3405 ; 0960-894X
ISSN (online)	1464-3405
ISSN	0960-894X
DOI	10.1016/j.bmcl.2017.11.015
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