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Article ; Online: Genetic Variants Associated with the Risk of Stroke in Sickle Cell Anemia: Systematic Review and Meta-Analysis.

Kumari, Aradhana / Chauhan, Ganesh / Chaudhuri, Partha Kumar / Kumari, Sushma / Prasad, Anupa

2024 Volume 48, Issue 2, Page(s) 101–112

Abstract: Sickle cell anemia (SCA) is the most common cause of stroke in children. As it is a rare disease, studies investigating the association with complications like stroke in SCD have small sample sizes. Here, we performed a systematic review and meta- ... ...

Abstract	Sickle cell anemia (SCA) is the most common cause of stroke in children. As it is a rare disease, studies investigating the association with complications like stroke in SCD have small sample sizes. Here, we performed a systematic review and meta-analysis of the studies exploring an association of genetic variants with stroke to get a better indication of their association with stroke. PubMed and Google Scholar were searched to identify studies that had performed an association analysis of genetic variants for the risk of stroke in SCA patients. After screening of eligible studies, summary statistics of association analysis with stroke and other general information were extracted. Meta-analysis was performed using the fixed effect method on the tool METAL and forest plots were plotted using the R program. The random effect model was performed as a sensitivity analysis for loci where significant heterogeneity was observed. 407 studies were identified using the search term and after screening 37 studies that cumulatively analyzed 11,373 SCA patients were included. These 37 studies included a total of 2,222 SCA patients with stroke, predominantly included individuals of African ancestry (N = 16). Three of these studies performed whole exome sequencing while 35 performed single nucleotide-based genotyping. Though the studies reported association with 132 loci, meta-analyses could be performed only for 12 loci that had data from two or more studies. After meta-analysis we observed that four loci were significantly associated with risk for stroke: -α3.7 kb
MeSH term(s)	Anemia, Sickle Cell/genetics ; Anemia, Sickle Cell/complications ; Humans ; Stroke/genetics ; Stroke/epidemiology ; Stroke/etiology ; Genetic Predisposition to Disease ; Genetic Variation ; Polymorphism, Single Nucleotide ; Risk Factors
Language	English
Publishing date	2024-04-18
Publishing country	England
Document type	Journal Article ; Systematic Review ; Meta-Analysis
ZDB-ID	750615-6
ISSN	1532-432X ; 0363-0269
ISSN (online)	1532-432X
ISSN	0363-0269
DOI	10.1080/03630269.2024.2340685
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Article: Prospective Observational Study of Early Tracheostomy Role in Operated Severe Head Injury Patients at A Level 1 Trauma Center.

Bharti, Rohit / Sindhu, Sindhu / Sundaram, Ponraj K / Chauhan, Ganesh

Bulletin of emergency and trauma

2021 Volume 9, Issue 4, Page(s) 188–194

Abstract: Objective: To evaluate the impact of the early tracheostomy on operated patients with severe head injury.: Methods: This prospective observational study was conducted at a level 1 trauma center and medical college over one-year period. The study ... ...

Abstract	Objective: To evaluate the impact of the early tracheostomy on operated patients with severe head injury. Methods: This prospective observational study was conducted at a level 1 trauma center and medical college over one-year period. The study included all surgically managed severe head injury patients without any other life-threatening major injuries. Patients who underwent tracheostomy within 7 days were classified as early tracheostomy. Results: The patient's mean age of this cohort study was 43.4±14.5 years. Motor-vehicle accidents were being the most common cause of severe head injury. Operated patients were undergoing early tracheostomy on an average of 2.9 days. We were observed that the patients spent on a mechanical ventilation on an average 3.67±2.26 days. This was significantly lower than previous four published studies ( Conclusion: We have shown that it is possible to decrease mechanical ventilation (MV) time, intensive care unit (ICU) stay and total hospital stay by doing early tracheostomy in operated severe head injury patients.
Language	English
Publishing date	2021-10-20
Publishing country	Iran
Document type	Journal Article
ZDB-ID	2722734-0
ISSN	2322-3960 ; 2322-2522
ISSN (online)	2322-3960
ISSN	2322-2522
DOI	10.30476/BEAT.2021.86725.1198
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Article: A comparative study between bone transport technique using Ilizarov/LRS fixator and induced membrane (Masquelet) technique in management of bone defects in the long bones of lower limb.

Gupta, Govind Kumar / Majhee, Amit Kumar / Rani, Sudha / Shekhar, Shubhendu / Prasad, Pancham / Chauhan, Ganesh

Journal of family medicine and primary care

2022 Volume 11, Issue 7, Page(s) 3660–3666

Abstract: Introduction: In earlier times due to difficulty in managing segmental long bone defects, amputation was the preferred treatment. Nonunion with bone loss of long bones is a challenging problem, requiring serious attention. Post-traumatic segmental bone ... ...

Abstract	Introduction: In earlier times due to difficulty in managing segmental long bone defects, amputation was the preferred treatment. Nonunion with bone loss of long bones is a challenging problem, requiring serious attention. Post-traumatic segmental bone defects can have severe long-term ill impact on patient's lives. Reconstruction is more difficult and functional outcome is usually less satisfactory compared to bony outcome. Distraction osteogenesis and induced membrane technique are the techniques that can be used. Aims and objectives: To find out and compare clinical, radiological, and functional outcome of bone transport technique and induced membrane technique in management of bone defects in the long bones of lower limb. Materials and methods: A comparative study was conducted on 24 patients (22 males and 2 females) of lower extremity fractures with bone defect more than 3 cm. Patients were divided into two groups according to the method of reconstruction used, that is, either bone transport technique in 12 patients (group A) or masquelet in the other 12 patients (group B). The mean age of the patients was 44 years in group A and was 38 years in group B. Regular follow-up was done with a mean period of follow up of 18.35 ± 5.58 months in group A and 18.25 ± 3.95 months in group B. Result: In group A (bone transport), 67% showed union, 25% showed union with bone graft and 8% showed delayed union. In group B (masquelet), 75% showed union and 25% showed delayed union. bone transport technique showed excellent results in 58.3% and good in 41.7% while Masquelet technique showed excellent result in 50% and good in 50%. Conclusion: For an orthopaedic surgeon, long bones defects with a substantial loss of bone volume are one of the most challenging bone defects encountered in clinical practice. Induced membrane technique and bone transport both offer successful options for filling of bone defects. Both techniques have its own pros and cons and provide varied option for healing. In our study, both methods have comparable results statistically although induced membrane technique required soft tissue reconstructive procedures.
Language	English
Publishing date	2022-07-22
Publishing country	India
Document type	Journal Article
ZDB-ID	2735275-4
ISSN	2278-7135 ; 2249-4863
ISSN (online)	2278-7135
ISSN	2249-4863
DOI	10.4103/jfmpc.jfmpc_2447_21
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Article ; Online: Genetic Risk Factors for Ischemic and Hemorrhagic Stroke.

Chauhan, Ganesh / Debette, Stéphanie

Current cardiology reports

2016 Volume 18, Issue 12, Page(s) 124

Abstract: Understanding the genetic risk factors for stroke is an essential step to decipher the underlying mechanisms, facilitate the identification of novel therapeutic targets, and optimize the design of prevention strategies. A very small proportion of strokes ...

Abstract	Understanding the genetic risk factors for stroke is an essential step to decipher the underlying mechanisms, facilitate the identification of novel therapeutic targets, and optimize the design of prevention strategies. A very small proportion of strokes are attributable to monogenic conditions, the vast majority being multifactorial, with multiple genetic and environmental risk factors of small effect size. Genome-wide association studies and large international consortia have been instrumental in finding genetic risk factors for stroke. While initial studies identified risk loci for specific stroke subtypes, more recent studies also revealed loci associated with all stroke and all ischemic stroke. Risk loci for ischemic stroke and its subtypes have been implicated in atrial fibrillation (PITX2 and ZFHX3), coronary artery disease (ABO, chr9p21, HDAC9, and ALDH2), blood pressure (ALDH2 and HDAC9), pericyte and smooth muscle cell development (FOXF2), coagulation (HABP2), carotid plaque formation (MMP12), and neuro-inflammation (TSPAN2). For hemorrhagic stroke, two loci (APOE and PMF1) have been identified.
MeSH term(s)	Atrial Fibrillation/epidemiology ; Atrial Fibrillation/genetics ; Atrial Fibrillation/physiopathology ; Brain Ischemia/epidemiology ; Brain Ischemia/genetics ; Brain Ischemia/physiopathology ; Genetic Predisposition to Disease/genetics ; Genome-Wide Association Study ; Humans ; Polymorphism, Single Nucleotide/genetics ; Risk Factors ; Stroke/epidemiology ; Stroke/genetics ; Stroke/physiopathology
Language	English
Publishing date	2016-12
Publishing country	United States
Document type	Journal Article ; Review
ZDB-ID	2055373-0
ISSN	1534-3170 ; 1523-3782
ISSN (online)	1534-3170
ISSN	1523-3782
DOI	10.1007/s11886-016-0804-z
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Article: Complications of Different Types of Cranioplasty and Identification of Risk Factors Associated with Cranioplasty at a Tertiary Care Centre: A Prospective Observational Study

Shukla, Yatendra / Sundaram, Ponraj Kamatchi / Ramalingam, Jinendra Kumar / Costa, Jorson D. / Parab, Amey / Jakhar, Subhash / Chauhan, Ganesh / Bharti, Rohit

Indian Journal of Neurosurgery

2023 Volume 13, Issue 01, Page(s) 27–34

Abstract: Objective: Decompressive craniectomy (DC) is an urgent procedure which is done to decrease intracranial pressure. A study of the complications would suggest measures to improve the care. This study was focused on analyzing the complications after ... ...

Abstract	Objective: Decompressive craniectomy (DC) is an urgent procedure which is done to decrease intracranial pressure. A study of the complications would suggest measures to improve the care. This study was focused on analyzing the complications after cranioplasty (CP) and to identify risk factors that may be associated with the failure of the procedure. Method: This study was conducted over 41 months at level-1 trauma center and medical college. It included patients undergoing CP for a defect arising out of previous DC or inability to replace the bone flap (Glasgow Coma Scale > 13, size > 5 cm, without surgical site infection). All patients underwent CT imaging before and after the procedure. The CP technique largely depended on the patients, based on the cost of prosthesis and availability. Result: Hundred patients were included in the study. Postoperatively, total 22 patients suffered complications of which few had more than one complication. Titanium implant appeared to be a better implant, with no complication ( p < 0.05). Complication was common in younger age group, chemically cured PMMA (polymethyl methacrylate) and ethylene oxide) sterilized bone flap). Complications were higher among patients with multiple comorbidity and stroke patients. Conclusion: Titanium flap had no complication and in case of autologous abdominal subcutaneous flap, apart from bone flap absorption, patients had no major complication. Therefore, both implants are preferred implants for CP. Heat-cured PMMA can be used in case of nonavailability of a better option, as it is economical feasible and can be molded at any dental lab.
Keywords	Decompressive craniectomy ; cranioplasty ; infection ; titanium ; methyl methacrylate
Language	English
Publishing date	2023-02-23
Publisher	Thieme Medical and Scientific Publishers Pvt. Ltd.
Publishing place	Stuttgart ; New York
Document type	Article
ZDB-ID	2698542-1
ISSN	2277-9167 ; 2277-954X ; 2277-9167
ISSN (online)	2277-9167
ISSN	2277-954X ; 2277-9167
DOI	10.1055/s-0043-1761603
Database	Thieme publisher's database

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Article ; Online: Procalcitonin as a predictive marker in COVID-19: A systematic review and meta-analysis.

Kumar, Amit / Karn, Era / Trivedi, Kiran / Kumar, Pramod / Chauhan, Ganesh / Kumari, Aradhana / Pant, Pragya / Munisamy, Murali / Prakash, Jay / Sarkar, Prattay Guha / Prasad, Kameshwar / Prasad, Anupa

PloS one

2022 Volume 17, Issue 9, Page(s) e0272840

Abstract: Background: Coronavirus disease 2019 has emerged as a global pandemic causing millions of critical cases and deaths. Early identification of at-risk patients is crucial for planning triage and treatment strategies.: Methods and findings: We performed ...

Abstract	Background: Coronavirus disease 2019 has emerged as a global pandemic causing millions of critical cases and deaths. Early identification of at-risk patients is crucial for planning triage and treatment strategies. Methods and findings: We performed this systematic review and meta-analysis to determine the pooled prognostic significance of procalcitonin in predicting mortality and severity in patients with COVID-19 using a robust methodology and clear clinical implications. Design: We used Preferred Reporting Items for Systematic Reviews and Meta-Analyses and Cochrane Handbook for Systematic Reviews of Interventions guidelines. We included thirty-two prospective and retrospective cohort studies involving 13,154 patients. Results: The diagnostic odds ratio of procalcitonin for predicting mortality were estimated to be 11 (95% CI: 7 to 17) with sensitivity, specificity, and summary area under the curveof 0.83 (95% CI: 0.70 to 0.91), 0.69 (95% CI: 0.58 to 0.79), and 0.83 (95% CI: 0.79 to 0.86) respectively. While for identifying severe cases of COVID-19, the odds ratio was 8.0 (95% CI 5.0 to 12.0) with sensitivity, specificity, and summary area under the curve of 0.73 (95% CI 0.67 to 0.78), 0.74 (0.66 to 0.81), and 0.78 (95% CI 0.74 to 0.82) respectively. Conclusion: Procalcitonin has good discriminatory power for predicting mortality and disease severity in COVID-19 patients. Therefore, procalcitonin measurement may help identify potentially severe cases and thus decrease mortality by offering early aggressive treatment.
MeSH term(s)	Biomarkers ; COVID-19/diagnosis ; Humans ; Pandemics ; Procalcitonin ; Prospective Studies ; Retrospective Studies
Chemical Substances	Biomarkers ; Procalcitonin
Language	English
Publishing date	2022-09-09
Publishing country	United States
Document type	Journal Article ; Meta-Analysis ; Systematic Review
ZDB-ID	2267670-3
ISSN	1932-6203 ; 1932-6203
ISSN (online)	1932-6203
ISSN	1932-6203
DOI	10.1371/journal.pone.0272840
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Book: Kārāmad gharelū g̲h̲izāī nusk̲h̲e

Chauhān, Ganesh Nārāyan

g̲h̲izā ke z̲arīʻe ʻilāj par ʻamal aur mufaṣṣal kitāb

2000

Author's details	Ganesh Nārāyan Chauhān
MeSH term(s)	Medicine, Ayurvedic ; Medicine, Unani ; Disease
Language	Urdu
Size	245 p.
Edition	Bār 1.
Publisher	Al-K̲h̲ayyām Pablisharz
Publishing place	Lāhaur
Document type	Book
Note	In Urdu.
Database	Catalogue of the US National Library of Medicine (NLM)

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Article ; Online: Association of SUMOylation Pathway Genes With Stroke in a Genome-Wide Association Study in India.

Kumar, Amit / Chauhan, Ganesh / Sharma, Shriram / Dabla, Surekha / Sylaja, P N / Chaudhary, Neera / Gupta, Salil / Agrawal, Chandra Sekhar / Anand, Kuljeet Singh / Srivastava, Achal Kumar / Vibha, Deepti / Sagar, Ram / Raj, Ritesh / Maheshwari, Ankita / Vivekanandhan, Subbiah / Kaul, Bhavna / Raghavan, Samudrala / Gorthi, Sankar Prasad / Mohania, Dheeraj /

Kaushik, Samander / Yadav, Rohtas Kanwar / Hazarika, Anjali / Sharma, Pankaj / Prasad, Kameshwar

Neurology

2021 Volume 97, Issue 4, Page(s) e345–e356

Abstract: Objective: To undertake a genome-wide association study (GWAS) to identify genetic variants for stroke in an Indian population.: Methods: In a hospital-based case-control study, 8 teaching hospitals in India recruited 4,088 participants, including 1, ... ...

Abstract	Objective: To undertake a genome-wide association study (GWAS) to identify genetic variants for stroke in an Indian population. Methods: In a hospital-based case-control study, 8 teaching hospitals in India recruited 4,088 participants, including 1,609 stroke cases. Imputed genetic variants were tested for association with stroke subtypes using both single-marker and gene-based tests. Association with vascular risk factors was performed with logistic regression. Various databases were searched for replication, functional annotation, and association with related traits. Status of candidate genes previously reported in the Indian population was also checked. Results: Associations of vascular risk factors with stroke were similar to previous reports and show modifiable risk factors such as hypertension, smoking, and alcohol consumption as having the highest effect. Single-marker-based association revealed 2 loci for cardioembolic stroke (1p21 and 16q24), 2 for small vessel disease stroke (3p26 and 16p13), and 4 for hemorrhagic stroke (3q24, 5q33, 6q13, and 19q13) at Conclusions: This GWAS of stroke in an Indian population identified novel loci and replicated previously known loci. Genetic variants in the SUMOylation pathway, which has been implicated in brain ischemia, were identified for association with stroke.
MeSH term(s)	Adult ; Aged ; Alleles ; Brain Ischemia/genetics ; Case-Control Studies ; Female ; Genetic Predisposition to Disease ; Genome-Wide Association Study ; Humans ; India ; Male ; Middle Aged ; Phenotype ; Polymorphism, Single Nucleotide ; Quantitative Trait Loci ; Risk Factors ; Stroke/genetics ; Sumoylation
Language	English
Publishing date	2021-05-24
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	207147-2
ISSN	1526-632X ; 0028-3878
ISSN (online)	1526-632X
ISSN	0028-3878
DOI	10.1212/WNL.0000000000012258
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Article ; Online: Evaluation of DOK5 as a susceptibility gene for type 2 diabetes and obesity in North Indian population

Tandon Nikhil / Ghosh Saurabh / Dwivedi Om / Chauhan Ganesh / Mahajan Anubha / Tabassum Rubina / Bharadwaj Dwaipayan

BMC Medical Genetics, Vol 11, Iss 1, p

2010 Volume 35

Abstract: Abstract Background Type 2 diabetes is a complex metabolic disorder with obesity being a major contributing factor in its development. Susceptibility loci for type 2 diabetes and obesity have been localized on different chromosomal regions by various ... ...

Abstract	Abstract Background Type 2 diabetes is a complex metabolic disorder with obesity being a major contributing factor in its development. Susceptibility loci for type 2 diabetes and obesity have been localized on different chromosomal regions by various genome-wide linkage scans. Of these chromosomal regions, 20q13 is one of the strongest linked regions for type 2 diabetes as well as obesity. On 20q13 lies DOK5 that seems to be a strong functional and positional candidate for type 2 diabetes and obesity because of its involvement in insulin signaling and immune responses. Hence, for the first time, we explored DOK5 as a potential type 2 diabetes and obesity susceptibility gene. Methods We sequenced 43 subjects for polymorphisms in functionally relevant regions of DOK5 . A total of 10 SNPs that included 5 that were identified by sequencing and 5 additional SNPs from NCBI Variation Database were genotyped in 2,115 participants comprising of 1,073 patients with type 2 diabetes and 1,042 controls of Indo-European ethnicity from North India. Results We identified a novel variant in intron 7 referred to as DK176673. We found nominal association of three SNPs-rs6064099 (OR = 0.75, P = 0.019), rs873079 (OR = 0.76, P = 0.036) and DK176673 (OR = 1.55, P = 0.037) with type 2 diabetes among normal-weight subjects [BMI < 23 kg/m 2 ]. The haplotype GGC harboring rs6068916, rs6064099 and rs873079 showed strong association with type 2 diabetes among normal-weight subjects (OR = 1.37, P / P perm = 5.8 × 10 -3 /0.037). Association analysis with obesity revealed that rs6064099 is associated with reduced susceptibility for obesity (OR = 0.48, P = 6.8 × 10 -3 ). Also, haplotype GGC conferred increased susceptibility for obesity (OR = 1.27, P / P perm = 9.0 × 10 -3 /0.039). Also, rs6064099 was significantly associated with reduced BMI [median(IQR) = 24.0(20.7-27.1) vs 23.9(20.2-26.8) vs 21.8(19.2-24.7) for GG vs GC vs CC, P = 7.0 × 10 -3 ]. Conclusions We identified DOK5 as a novel susceptibility gene for obesity and type 2 diabetes ...
Keywords	Internal medicine ; RC31-1245 ; Genetics ; QH426-470
Subject code	616
Language	English
Publishing date	2010-02-01T00:00:00Z
Publisher	BMC
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Burden of Dilated Perivascular Spaces, an Emerging Marker of Cerebral Small Vessel Disease, Is Highly Heritable.

Duperron, Marie-Gabrielle / Tzourio, Christophe / Sargurupremraj, Muralidharan / Mazoyer, Bernard / Soumaré, Aïcha / Schilling, Sabrina / Amouyel, Philippe / Chauhan, Ganesh / Zhu, Yi-Cheng / Debette, Stéphanie

Stroke

2018 Volume 49, Issue 2, Page(s) 282–287

Abstract: Background and purpose: The genetic contribution to dilated perivascular space (dPVS) burden-an emerging MRI marker of cerebral small vessel disease-is unknown. We measured the heritability of dPVS burden and its shared heritability with other MRI ... ...

Abstract	Background and purpose: The genetic contribution to dilated perivascular space (dPVS) burden-an emerging MRI marker of cerebral small vessel disease-is unknown. We measured the heritability of dPVS burden and its shared heritability with other MRI markers of cerebral small vessel disease. Methods: The study sample comprised 1597 participants from the population-based Three City (3C) Dijon Study, with brain MRI and genome-wide genotyping (mean age, 72.8±4.1 years; 61% women). dPVS burden and lacunar brain infarcts were rated on a semiquantitative scale, whereas an automated algorithm generated white matter hyperintensity volume (WMHV). We estimated dPVS burden heritability and shared heritability with WMHV and lacunar brain infarcts using the genome-wide complex trait analysis tool, on unrelated participants, adjusting for age, sex, intracranial volume, and principal components of population stratification. Results: dPVS burden was significantly correlated with WMHV and lacunar brain infarcts, the strongest correlation being found between WMHV and dPVS in basal ganglia. Heritability estimates were h Conclusions: We provide evidence for important genetic contribution to dPVS burden in older community-dwelling people, some of which may be shared with WMHV. Differential heritability patterns for dPVS in white matter and basal ganglia suggest at least partly distinct underlying biological processes.
MeSH term(s)	Aged ; Aged, 80 and over ; Brain/blood supply ; Cerebral Small Vessel Diseases/complications ; Cerebral Small Vessel Diseases/genetics ; Female ; Genotype ; Humans ; Magnetic Resonance Imaging/methods ; Male ; Risk Factors ; Stroke, Lacunar/complications ; Stroke, Lacunar/genetics
Language	English
Publishing date	2018
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	80381-9
ISSN	1524-4628 ; 0039-2499 ; 0749-7954
ISSN (online)	1524-4628
ISSN	0039-2499 ; 0749-7954
DOI	10.1161/STROKEAHA.117.019309
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