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  1. Article ; Online: Effects of Inclined Interface Angle on Compressible Rayleigh-Taylor Instability: A Numerical Study Based on the Discrete Boltzmann Method.

    Chen, Bailing / Lai, Huilin / Lin, Chuandong / Li, Demei

    Entropy (Basel, Switzerland)

    2023  Volume 25, Issue 12

    Abstract: Rayleigh-Taylor (RT) instability is a basic fluid interface instability that widely exists in nature and in the engineering field. To investigate the impact of the initial inclined interface on compressible RT instability, the two-component discrete ... ...

    Abstract Rayleigh-Taylor (RT) instability is a basic fluid interface instability that widely exists in nature and in the engineering field. To investigate the impact of the initial inclined interface on compressible RT instability, the two-component discrete Boltzmann method is employed. Both the thermodynamic non-equilibrium (TNE) and hydrodynamic non-equilibrium (HNE) effects are studied. It can be found that the global average density gradient in the horizontal direction, the non-organized energy fluxes, the global average non-equilibrium intensity and the proportion of the non-equilibrium region first increase and then reduce with time. However, the global average density gradient in the vertical direction and the non-organized moment fluxes first descend, then rise, and finally descend. Furthermore, the global average density gradient, the typical TNE intensity and the proportion of non-equilibrium region increase with increasing angle of the initial inclined interface. Physically, there are three competitive mechanisms: (1) As the perturbed interface elongates, the contact area between the two fluids expands, which results in an increasing gradient of macroscopic physical quantities and leads to a strengthening of the TNE effects. (2) Under the influence of viscosity, the perturbation pressure waves on both sides of the material interface decrease with time, which makes the gradient of the macroscopic physical quantity decrease, resulting in a weakening of the TNE strength. (3) Due to dissipation and/or mutual penetration of the two fluids, the gradient of macroscopic physical quantities gradually diminishes, resulting in a decrease in the intensity of the TNE.
    Language English
    Publishing date 2023-12-05
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2014734-X
    ISSN 1099-4300 ; 1099-4300
    ISSN (online) 1099-4300
    ISSN 1099-4300
    DOI 10.3390/e25121623
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: A novel fault diagnosis strategy for LED lamps via light output time-frequency characteristics analysis and machine learning.

    Shang, Yuhang / Sun, Fukang / Fang, Qiansheng / Chen, Bailing / Xie, Jianxia

    Heliyon

    2023  Volume 9, Issue 9, Page(s) e19737

    Abstract: Faulty LED lamps can cause a decrease in light efficiency, lead to flicker, and have a negative impact on creating a reliable, stable, and healthy light environment. However, many LED lamps' faults are difficult to detect by electrical parameter ... ...

    Abstract Faulty LED lamps can cause a decrease in light efficiency, lead to flicker, and have a negative impact on creating a reliable, stable, and healthy light environment. However, many LED lamps' faults are difficult to detect by electrical parameter measurements or naked-eye observation. Consequently, in this paper, a novel fault diagnosis strategy is proposed by analyzing light output time-frequency characteristics of LED lamps. The proposed fault diagnosis strategy contains three stages: (1) collecting the light output signal of LED lamps, (2) extracting the light output time-frequency characteristics of LED lamps by VMD and energy entropy calculation, and (3) employing SVM to construct the fault diagnosis model which used to identify the faulty LED lamps. To validate the feasibility and effectiveness of the proposed fault diagnosis strategy, simulation experiments are conducted, and the light output signals of LED lamps are collected as experiment datasets using the 10 kHz sampling frequency. The results demonstrate that the proposed fault diagnosis strategy can identify faults effectively, and average accuracy rate can reach to over 92%. This study can help promote the development of large-scale LED lamp maintenance management technology, and bring great benefits for the reliable and healthy operation of large-scale LED lamps particularly.
    Language English
    Publishing date 2023-09-01
    Publishing country England
    Document type Journal Article
    ZDB-ID 2835763-2
    ISSN 2405-8440
    ISSN 2405-8440
    DOI 10.1016/j.heliyon.2023.e19737
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Linkage mapping and QTL analysis of growth traits in Rhopilema esculentum.

    Chen, Bailing / Li, Yulong / Tian, Meilin / Su, Hao / Sun, Wei / Li, Yunfeng

    Scientific reports

    2022  Volume 12, Issue 1, Page(s) 471

    Abstract: R. esculentum is a popular seafood in Asian countries and an economic marine fishery resource in China. However, the genetic linkage map and growth-related molecular markers are still lacking, hindering marker assisted selection (MAS) for genetic ... ...

    Abstract R. esculentum is a popular seafood in Asian countries and an economic marine fishery resource in China. However, the genetic linkage map and growth-related molecular markers are still lacking, hindering marker assisted selection (MAS) for genetic improvement of R. esculentum. Therefore, we firstly used 2b-restriction site-associated DNA (2b-RAD) method to sequence 152 R. esculentum specimens and obtained 9100 single nucleotide polymorphism (SNP) markers. A 1456.34 cM linkage map was constructed using 2508 SNP markers with an average interval of 0.58 cM. Then, six quantitative trait loci (QTLs) for umbrella diameter and body weight were detected by QTL analysis based on the new linkage map. The six QTLs are located on four linkage groups (LGs), LG4, LG13, LG14 and LG15, explaining 9.4% to 13.4% of the phenotypic variation. Finally, 27 candidate genes in QTLs regions of LG 14 and 15 were found associated with growth and one gene named RE13670 (sushi, von Willebrand factor type A, EGF and pentraxin domain-containing protein 1-like) may play an important role in controlling the growth of R. esculentum. This study provides valuable information for investigating the growth mechanism and MAS breeding in R. esculentum.
    MeSH term(s) Animals ; Chromosome Mapping ; Phenotype ; Polymorphism, Single Nucleotide ; Quantitative Trait Loci ; Scyphozoa/classification ; Scyphozoa/genetics ; Scyphozoa/growth & development
    Language English
    Publishing date 2022-01-10
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-021-04431-0
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Cyclosporin-A reduced the cytotoxicity of propranolol in HUVECs via p38 MAPK signaling.

    Lv, Zhong / Xie, Guanhao / Cui, Haowen / Yao, Zhi / Shao, Congxiang / Yuan, Weiquan / Chen, Bailing

    Medicine

    2022  Volume 101, Issue 4, Page(s) e28329

    Abstract: Abstract: Propranolol (PROP) is a nonselective β-adrenergic receptor antagonist used to treat hypertension and cardiac arrhythmias. Oral administration of PROP has recently emerged as a new treatment modality for hemangiomas. However, the side effects ... ...

    Abstract Abstract: Propranolol (PROP) is a nonselective β-adrenergic receptor antagonist used to treat hypertension and cardiac arrhythmias. Oral administration of PROP has recently emerged as a new treatment modality for hemangiomas. However, the side effects of PROP at the cellular level have not been adequately described.The present study investigates and highlights the mechanisms of coupling of the drugs cyclosporin-A (CyA) and PROP on cell proliferation and the occurrence of apoptosis. It also relays the antioxidant effect of PROP on human umbilical vein endothelial cells (HUVECs).HUVECs were treated with CyA and PROP. At 24 hours after treatment, the levels of reactive oxygen species (ROS), cell proliferation, and apoptosis were determined using the ROS kit, MTT assay, and Annexin V staining. In addition, the related proteins of phospho-p38 mitogen-activated protein kinase were determined by western blotting. Subsequently, HUVECs pretreated with CyA or PROP were treated with the p38 inhibitor (SB203580). Finally, the ROS level, cell proliferation, and apoptosis were measured again in both active HUVECs and HUVECs, in which the p38 proteins were inhibited.The combination of CyA and PROP reversed the effect of CyA on cell viability, reduced the ROS level and the cell apoptosis induced by PROP. Moreover, inhibition of p38 protein catalase activity immediately stopped the effect of CyA-propranolol in HUVECs.The effect of the CyA-propranolol combination on HUVECs is associated with the p38 pathway changes, which is proven to be a potential chemotherapeutic agent that minimizes the side effects of PROP in hemangioma therapy.
    MeSH term(s) Antioxidants/pharmacology ; Apoptosis/drug effects ; Cell Proliferation/drug effects ; Cyclosporine/pharmacology ; Hemangioma/drug therapy ; Human Umbilical Vein Endothelial Cells/drug effects ; Humans ; Propranolol/pharmacology ; Reactive Oxygen Species ; p38 Mitogen-Activated Protein Kinases
    Chemical Substances Antioxidants ; Reactive Oxygen Species ; Cyclosporine (83HN0GTJ6D) ; Propranolol (9Y8NXQ24VQ) ; p38 Mitogen-Activated Protein Kinases (EC 2.7.11.24)
    Language English
    Publishing date 2022-01-04
    Publishing country United States
    Document type Journal Article
    ZDB-ID 80184-7
    ISSN 1536-5964 ; 0025-7974
    ISSN (online) 1536-5964
    ISSN 0025-7974
    DOI 10.1097/MD.0000000000028329
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Greater Consumption of Total and Individual Lignans and Dietary Fibers Were Significantly Associated with Lowered Risk of Hip Fracture—A 1:1 Matched Case–Control Study among Chinese Elderly Men and Women

    Liu, Zhaomin / Chen, Bailing / Li, Baolin / Wang, Cheng / Li, Guoyi / Cao, Wenting / Zeng, Fangfang / Chen, Yuming

    Nutrients. 2022 Mar. 05, v. 14, no. 5

    2022  

    Abstract: The study aims to examine the association of dietary intake of lignans with the risk of hip fractures in Chinese older adults. This was a 1:1 age- and gender- matched case–control study. Dietary survey was conducted by face-to-face interviews using a 79- ... ...

    Abstract The study aims to examine the association of dietary intake of lignans with the risk of hip fractures in Chinese older adults. This was a 1:1 age- and gender- matched case–control study. Dietary survey was conducted by face-to-face interviews using a 79-item validated food frequency questionnaire. Habitual intake of total and individual lignans (matairesinol, secoisolariciresinol, pinoresinol, and lariciresinol) was estimated based on the available lignans databases. Conditional logistic regression was used to examine the relationship of dietary total and individual lignans, lignan-rich foods (vegetables, fruits, nuts, and cereals) and dietary fibers with the risk of hip fracture. A total of 1070 pairs of hip fracture incident cases and controls were recruited. Compared with the lowest quartile, the highest quartile group showed a reduced hip fracture risk by 76.3% (0.237, 95% CI: 0.103–0.544, Pₜᵣₑₙd < 0.001) for total lignans, and 62.5% (0.375, 95% CI: 0.194–0.724, Pₜᵣₑₙd = 0.001) for dietary fibers. Similar findings were observed for individual lignans, the estimated enterolactone level, as well as lignans from vegetables and nuts. We concluded that greater consumption of total and individual lignans, and lignan-rich foods were significantly associated with decreased risk of hip fracture.
    Keywords case-control studies ; dietary surveys ; elderly ; food frequency questionnaires ; food intake ; hip fractures ; lignans ; matairesinol ; regression analysis ; risk ; risk reduction
    Language English
    Dates of publication 2022-0305
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article
    ZDB-ID 2518386-2
    ISSN 2072-6643
    ISSN 2072-6643
    DOI 10.3390/nu14051100
    Database NAL-Catalogue (AGRICOLA)

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  6. Article ; Online: Structure of Lumbar Intervertebral Foraminal Ligaments Based on 3-Dimensional Reconstruction Through Ultrathin Cryomilling of a Human Cadaver.

    Zhao, Shengli / Min, Shaoxiong / Wu, Penghuan / Huang, Chengyan / Zhao, Qinghao / Chen, Bailing / Shi, Benchao

    World neurosurgery

    2021  Volume 148, Page(s) e609–e616

    Abstract: Background: This study aimed to assess the feasibility of using the 3-dimensional (3-D) reconstruction technique based on ultrathin cryomilling to show the lumbar intervertebral foraminal ligaments in situ.: Methods: Cryomilling was performed on an ... ...

    Abstract Background: This study aimed to assess the feasibility of using the 3-dimensional (3-D) reconstruction technique based on ultrathin cryomilling to show the lumbar intervertebral foraminal ligaments in situ.
    Methods: Cryomilling was performed on an embalmed human cadaver to acquire successive cross-sectional images. In each of the images, the boundaries of lumbar intervertebral foraminal ligaments and their adjacent structures were outlined, labeled, and reconstructed for 3-D modeling. The morphology, attachments, and spatial orientation of ligaments were described.
    Results: A total of 9 ligaments in 10 lumbar intervertebral foramina (IVFs) were identified and reconstructed. These ligaments can be divided into 5 types. The IVFs were divided into 2 or 3 main portions by the first 4 types of ligaments (transforaminal ligaments, corporotransverse ligaments, "reticular" ligaments, and "Y-shaped" ligaments). The radiating ligaments (the fifth type of ligaments) attached to the surrounding structures of the IVF and were connected directly to the nerve root sleeves. Although there was no indication of neurovascular compromise in this normal specimen, these ligaments limit the space within the bony IVF such that under certain pathologic conditions (e.g., inflammation), their presence would make neurovascular compression more likely than if they were absent.
    Conclusions: The 3-D reconstruction technique based on ultrathin cryomilling can effectively show the lumbar intervertebral foraminal ligaments and their anatomical characteristics in situ, providing a new way to clarify the relationships between these ligaments and their adjacent structures.
    MeSH term(s) Adult ; Anatomy, Cross-Sectional ; Cadaver ; Female ; Histological Techniques ; Humans ; Image Processing, Computer-Assisted ; Imaging, Three-Dimensional ; Intervertebral Disc/anatomy & histology ; Intervertebral Disc/diagnostic imaging ; Ligaments, Articular/anatomy & histology ; Ligaments, Articular/diagnostic imaging ; Lumbar Vertebrae/anatomy & histology ; Lumbar Vertebrae/diagnostic imaging ; Lumbosacral Region ; Spinal Nerve Roots/anatomy & histology ; Spinal Nerve Roots/diagnostic imaging
    Language English
    Publishing date 2021-01-19
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2534351-8
    ISSN 1878-8769 ; 1878-8750
    ISSN (online) 1878-8769
    ISSN 1878-8750
    DOI 10.1016/j.wneu.2021.01.032
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article: Osteocyte-specific dentin matrix protein 1 : the role of mineralization regulation in low-magnitude high-frequency vibration enhanced osteoporotic fracture healing.

    Li, Meng C M / Chow, Simon K-H / Wong, Ronald M Y / Chen, Bailing / Cheng, Jack C Y / Qin, Ling / Cheung, Wing-Hoi

    Bone & joint research

    2022  Volume 11, Issue 7, Page(s) 465–476

    Abstract: Aims: There is an increasing concern of osteoporotic fractures in the ageing population. Low-magnitude high-frequency vibration (LMHFV) was shown to significantly enhance osteoporotic fracture healing through alteration of osteocyte lacuno-canalicular ... ...

    Abstract Aims: There is an increasing concern of osteoporotic fractures in the ageing population. Low-magnitude high-frequency vibration (LMHFV) was shown to significantly enhance osteoporotic fracture healing through alteration of osteocyte lacuno-canalicular network (LCN). Dentin matrix protein 1 (DMP1) in osteocytes is known to be responsible for maintaining the LCN and mineralization. This study aimed to investigate the role of osteocyte-specific DMP1 during osteoporotic fracture healing augmented by LMHFV.
    Methods: A metaphyseal fracture was created in the distal femur of ovariectomy-induced osteoporotic Sprague Dawley rats. Rats were randomized to five different groups: 1) DMP1 knockdown (KD), 2) DMP1 KD + vibration (VT), 3) Scramble + VT, 4) VT, and 5) control (CT), where KD was performed by injection of short hairpin RNA (shRNA) into marrow cavity; vibration treatment was conducted at 35 Hz, 0.3 g; 20 minutes/day, five days/week). Assessments included radiography, micro-CT, dynamic histomorphometry and immunohistochemistry on DMP1, sclerostin, E11, and fibroblast growth factor 23 (FGF23). In vitro, murine long bone osteocyte-Y4 (MLO-Y4) osteocyte-like cells were randomized as in vivo groupings. DMP1 KD was performed by transfecting cells with shRNA plasmid. Assessments included immunocytochemistry on osteocyte-specific markers as above, and mineralized nodule staining.
    Results: Healing capacities in DMP1 KD groups were impaired. Results showed that DMP1 KD significantly abolished vibration-enhanced fracture healing at week 6. DMP1 KD significantly altered the expression of osteocyte-specific markers. The lower mineralization rate in DMP1 KD groups indicated that DMP1 knockdown was associated with poor fracture healing process.
    Conclusion: The blockage of DMP1 would impair healing outcomes and negate LMHFV-induced enhancement on fracture healing. These findings reveal the importance of DMP1 in response to the mechanical signal during osteoporotic fracture healing. Cite this article:
    Language English
    Publishing date 2022-07-05
    Publishing country England
    Document type Journal Article
    ZDB-ID 2669244-2
    ISSN 2046-3758
    ISSN 2046-3758
    DOI 10.1302/2046-3758.117.BJR-2021-0476.R2
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Study on Risk Factors of Primary Non-traumatic OVCF in Chinese Elderly and a Novel Prediction Model.

    Wen, Zhenxing / Mo, Xiaoyi / Zhao, Shengli / Qi, Zhichao / Fu, Dan / Wen, Shifeng / Cheung, Wing Hoi / Chen, Bailing

    Orthopaedic surgery

    2022  Volume 14, Issue 11, Page(s) 2925–2938

    Abstract: Objective: Prevention of fragility fractures is one of the public health priorities worldwide, whilst the incidence of osteoporotic vertebral compression fractures (OVCF) continues to rise and lacks the corresponding accurate prediction model. This ... ...

    Abstract Objective: Prevention of fragility fractures is one of the public health priorities worldwide, whilst the incidence of osteoporotic vertebral compression fractures (OVCF) continues to rise and lacks the corresponding accurate prediction model. This study aimed to screen potential causes and risk factors for primary non-traumatic osteoporotic vertebral compression fractures (NTOVCF) in the elderly by characterizing a patient population with NTOVCF and comparing it with a population of osteoporotic patients.
    Methods: Between January 2013 and January 2022, 208 elderly patients with unequivocal evidence of bone fragility manifested as painful NTOVCF were enrolled, and compared with 220 patients with osteoporosis and no fractures. The demographic data, bone turnover markers, blood routine, serum biochemical values, and radiological findings were investigated. Differences between the fracture and non-fracture groups were analyzed, and variables significant in univariate analysis and correlation analysis were included in the logistic analysis to build the risk prediction model for osteoporotic vertebral fractures. Univariate analysis using student's t-tests for continuous variables or a chi-squared test for categorical variables was conducted to identify risk factors.
    Results: No significant differences were revealed regarding age, gender, BMI, smoking, alcohol consumption, blood glucose, propeptide of type I procollagen (P1NP), and N-terminal middle segment osteocalcin (N-MID) (P > 0.05). Parathyroid Hormone (PTH), 25(OH)D, serum albumin (ALB), hemoglobin (HB), bone mineral density (BMD), and cross-sectional area (CSA) of the paraspinal muscle in the fracture group were significantly lower than those in the control group; however, b-C-terminal telopeptide of type I collagen (β-CTX), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), non-prostatic acid phosphatase (NACP), and fatty degeneration ratio (FDR) were significantly higher than those in the control group (P < 0.05). Logistic regression analysis showed that ALB, HB, CSA, and BMD were negatively correlated with NTOVCF, while β-CTX, HDL-C, NACP, and FDR were positively correlated with NTOVCF.
    Conclusion: Decreased physical activity, anemia, hypoproteinemia, imbalances in bone metabolism, abnormal lipid metabolism, and degenerative and decreased muscle mass, were all risk factors for OVCF in the elderly, spontaneous fractures may be the consequence of cumulative declines in multiple physiological systems over the lifespan. Based on this risk model, timely detection of patients with high OVCF risk and implementation of targeted preventive measures is expected to improve the effect of fracture prevention.
    MeSH term(s) Female ; Humans ; Aged ; Fractures, Compression ; Osteoporosis, Postmenopausal ; Spinal Fractures ; Osteoporotic Fractures/epidemiology ; Bone Density ; Bone Diseases, Metabolic ; Risk Factors ; China/epidemiology ; Cholesterol
    Chemical Substances Cholesterol (97C5T2UQ7J)
    Language English
    Publishing date 2022-09-28
    Publishing country Australia
    Document type Journal Article
    ZDB-ID 2503162-4
    ISSN 1757-7861 ; 1757-7853 ; 1757-7861
    ISSN (online) 1757-7861 ; 1757-7853
    ISSN 1757-7861
    DOI 10.1111/os.13531
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Declining serum bone turnover markers are associated with the short-term positive change of lumbar spine bone mineral density in postmenopausal women.

    Zhao, Shengli / Mo, Xiaoyi / Wen, Zhenxing / Liu, Ming / Chen, Zhipeng / Lin, Wei / Huang, Zifang / Chen, Bailing

    Menopause (New York, N.Y.)

    2022  Volume 29, Issue 3, Page(s) 335–343

    Abstract: Objective: While serum bone turnover markers (BTMs) and bone mineral density (BMD) have been confirmed as useable risk assessment tools for postmenopausal osteoporosis, the associations between BTMs and BMD changes are still ambiguous. The aim of this ... ...

    Abstract Objective: While serum bone turnover markers (BTMs) and bone mineral density (BMD) have been confirmed as useable risk assessment tools for postmenopausal osteoporosis, the associations between BTMs and BMD changes are still ambiguous. The aim of this study was to explore the underlying associations between BTMs and BMD changes in postmenopausal women.
    Methods: Between January 2015 and October 2020, 135 postmenopausal women were retrospectively enrolled. They were divided into two groups according to lumbar spine (LS) 1-4 BMD change (1 y T-score minus baseline T-score, Group 1 [n = 36] < 0 and Group 2 [n = 99] ≥ 0). The changes of BTMs (N-terminal middle segment osteocalcin [N-MID], propeptide of type I procollagen [P1NP], and β-C-terminal telopeptide of type I collagen [β-CTX]) and their associations with LS 1-4 BMD change were analyzed. The biochemical indices and clinical parameters related with LS 1-4 BMD change were also evaluated.
    Results: The 1 year N-MID, P1NP, β-CTX and Phosphorus in Group 2 were lower than those in Group 1 (P < 0.05), their changes within 1 year were significantly negatively correlated with LS 1-4 BMD change (R2 = -0.200, P < 0.001; R2 = -0.230, P < 0.001; R2 = -0.186, P < 0.001; R2 = -0.044, P = 0.015; respectively). Except for the Phosphorus change (area under the curve [AUC] = 0.623), the changes of N-MID, P1NP, and β-CTX and their 1 year levels had similar AUC to diagnose the short-term LS 1-4 BMD change (AUC > 0.7 for all, with the AUC of 1 y P1NP being the largest at 0.803). Binary logistic regression analysis showed that the physical activity and drug intervention were the determinant factors for the LS 1-4 BMD change (odds ratio = 6.856, 95% confidence interval: 2.058-22.839, P = 0.002; odds ratio  = 5.114, 95% confidence interval: 1.551-16.864, P = 0.007; respectively).
    Conclusions: Declining N-MID, P1NP, β-CTX, and Phosphorus are associated with the short-term increase of LS 1-4 BMD within 1 year. Physical activity and drug intervention are factors significantly influencing the change of LS 1-4 BMD in postmenopausal women.
    MeSH term(s) Biomarkers ; Bone Density ; Bone Remodeling ; Collagen Type I ; Female ; Humans ; Osteoporosis, Postmenopausal/complications ; Postmenopause ; Retrospective Studies
    Chemical Substances Biomarkers ; Collagen Type I
    Language English
    Publishing date 2022-01-31
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1205262-0
    ISSN 1530-0374 ; 1072-3714
    ISSN (online) 1530-0374
    ISSN 1072-3714
    DOI 10.1097/GME.0000000000001920
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Comprehensive bioinformatics analysis reveals the hub genes and pathways associated with multiple myeloma.

    Zhao, Shengli / Mo, Xiaoyi / Wen, Zhenxing / Ren, Lijuan / Chen, Zhipeng / Lin, Wei / Wang, Qi / Min, Shaoxiong / Chen, Bailing

    Hematology (Amsterdam, Netherlands)

    2022  Volume 27, Issue 1, Page(s) 280–292

    Abstract: Purpose: While the prognosis of multiple myeloma (MM) has significantly improved over the last decade because of new treatment options, it remains incurable. Aetiological explanations and biological targets based on genomics may provide additional help ... ...

    Abstract Purpose: While the prognosis of multiple myeloma (MM) has significantly improved over the last decade because of new treatment options, it remains incurable. Aetiological explanations and biological targets based on genomics may provide additional help for rational disease intervention.
    Materials and methods: Three microarray datasets associated with MM were downloaded from the Gene Expression Omnibus (GEO) database. GSE125364 and GSE39754 were used as the training set, and GSE13591 was used as the verification set. The differentially expressed genes (DEGs) were obtained from the training set, and Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were performed to annotate their functions. The hub genes were derived from the combined results of a protein-protein interaction (PPI) network and weighted gene coexpression network analysis (WGCNA). The receiver operating characteristic (ROC) curves of hub genes were plotted to evaluate their clinical diagnostic value. Biological processes and signaling pathways associated with hub genes were explained by gene set enrichment analysis (GSEA).
    Results: A total of 1759 DEGs were identified. GO and KEGG pathway analyses suggested that the DEGs were related to the process of protein metabolism. RPN1, SEC61A1, SPCS1, SRPR, SRPRB, SSR1 and TRAM1 were proven to have clinical diagnostic value for MM. The GSEA results suggested that the hub genes were widely involved in the N-glycan biosynthesis pathway.
    Conclusion: The hub genes identified in this study can partially explain the potential molecular mechanisms of MM and serve as candidate biomarkers for disease diagnosis.
    MeSH term(s) Computational Biology/methods ; Genomics/methods ; Humans ; Multiple Myeloma/genetics ; Prognosis ; Signal Transduction
    Language English
    Publishing date 2022-02-23
    Publishing country England
    Document type Journal Article
    ZDB-ID 1341428-8
    ISSN 1607-8454 ; 1024-5332 ; 1024-5340
    ISSN (online) 1607-8454
    ISSN 1024-5332 ; 1024-5340
    DOI 10.1080/16078454.2022.2040123
    Database MEDical Literature Analysis and Retrieval System OnLINE

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