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Article ; Online: "Nationwide fever″ vs "Nationwide fear″ after optimizing the COVID-19 response in China.

Chen, Hongguang / Zhang, Konglai

2023 Volume 82, Page(s) 103499

MeSH term(s)	Humans ; COVID-19 ; Fear ; SARS-CoV-2 ; China/epidemiology
Language	English
Publishing date	2023-02-01
Publishing country	Netherlands
Document type	Letter
ZDB-ID	2456678-0
ISSN	1876-2026 ; 1876-2018
ISSN (online)	1876-2026
ISSN	1876-2018
DOI	10.1016/j.ajp.2023.103499
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Article ; Online: Observation of the Effects of Different Enzyme Digestion Time and Enzyme Digestion Methods on the Extraction of Primary Renal Tubular Epithelial Cells from Sprague-Dawley Suckling Mice.

Sun, Tian / Chen, Hongguang / Zhang, Zhenwang

Biochemical genetics

2024

Abstract: Renal tubular epithelial cells are one of the essential functional cells in the kidney. Optimizing the isolation and culture method of primary renal tubular epithelial cells from SD mammary rats provides better experimental materials for renal tubule- ... ...

Abstract	Renal tubular epithelial cells are one of the essential functional cells in the kidney. Optimizing the isolation and culture method of primary renal tubular epithelial cells from SD mammary rats provides better experimental materials for renal tubule-related studies, which is essential for studying the pathogenesis of renal diseases, especially diabetic nephropathy and drug screening. SD rat renal tubular epithelial cells were isolated and purified by 2.5-mg/ml collagenase II or 2 mg/ml trypsin + 2.5 mg/ml collagenase II enzymatic digestion. The isolation and purification were observed at different time points (15 min, 30 min, 45 min, and 60 min) to determine the optimal extraction time for the enzymatic digestion method. After comparing the two enzymatic methods, it was determined that the trypsin + collagenase II enzymatic method was more effective. The primary renal tubular epithelial cells extracted by the trypsin + collagenase II digestion method were identified by the marker Cytokeratin 18 of renal tubular epithelial cells at 45 min of digestion with high purity. We established a simple, efficient, and reproducible method for isolation and culture of renal tubular epithelial cells in SD mammary gland rats.
Language	English
Publishing date	2024-03-13
Publishing country	United States
Document type	Journal Article
ZDB-ID	2168-4
ISSN	1573-4927 ; 0006-2928
ISSN (online)	1573-4927
ISSN	0006-2928
DOI	10.1007/s10528-024-10729-1
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Zs.A 578: Show issues

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Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

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Article ; Online: Craniotomy hematoma was removed from the bone flap and multiple holes were drilled on the bone flap: A case report.

Zhu, Wei / Ma, Lei / Li, Tuo / Chen, Hongguang

Medicine

2023 Volume 102, Issue 21, Page(s) e33781

Abstract: Background: Chronic subdural hematoma (CSDH) is a common disease in neurosurgery department. Burr-hole drainage is the main surgical treatment. And the recurrence rate is as high as 25%.: Case report: In this case, a male patient with CSDH in the ... ...

Abstract	Background: Chronic subdural hematoma (CSDH) is a common disease in neurosurgery department. Burr-hole drainage is the main surgical treatment. And the recurrence rate is as high as 25%. Case report: In this case, a male patient with CSDH in the left frontotemporal parietal region underwent 2 drilling and drainage operations in the local hospital, but the hematoma recurred after operations. Being unable to bearing the repeated and progressive aggravation of headache, he came to our hospital for treatment. After considering the comprehensive situation, we use a new surgical method, removal of hematoma by drilling multiple holes in the lateral skull, to cure the patient. Conclusions: We get inspirations from the treatment of moyamoya disease surgery, through the bone holes the scalp forms many "meat column" like structures which have powerful capability in absorption, so the scalp could deep into the hematoma, then the CSDH could be cured. Provide a new surgical method for the treatment of refractory CSDH.
MeSH term(s)	Humans ; Male ; Craniotomy/adverse effects ; Craniotomy/methods ; Trephining/adverse effects ; Drainage/methods ; Headache ; Skull/surgery ; Hematoma, Subdural, Chronic/surgery ; Recurrence ; Treatment Outcome
Language	English
Publishing date	2023-05-24
Publishing country	United States
Document type	Case Reports ; Journal Article
ZDB-ID	80184-7
ISSN	1536-5964 ; 0025-7974
ISSN (online)	1536-5964
ISSN	0025-7974
DOI	10.1097/MD.0000000000033781
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Ua VI Zs.171: Show issues

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Article ; Online: Study on the interaction protein of transcription factor Smad3 based on TurboID proximity labeling technology.

Yan, Biao / Zeng, Ting / Liu, Xiaoshan / Guo, Yuanyuan / Chen, Hongguang / Guo, Shuang / Liu, Wu

Genomics

2024 Volume 116, Issue 3, Page(s) 110839

Abstract: TurboID is a highly efficient biotin-labelling enzyme, which can be used to explore a number of new intercalating proteins due to the very transient binding and catalytic functions of many proteins. TGF-β/Smad3 signaling pathway is involved in many ... ...

Abstract	TurboID is a highly efficient biotin-labelling enzyme, which can be used to explore a number of new intercalating proteins due to the very transient binding and catalytic functions of many proteins. TGF-β/Smad3 signaling pathway is involved in many diseases, especially in diabetic nephropathy and inflammation. In this paper, a stably cell line transfected with Smad3 were constructed by using lentiviral infection. To further investigate the function of TGF-β/Smad3, the protein labeling experiment was conducted to find the interacting protein with Smad3 gene. Label-free mass spectrometry analysis was performed to obtain 491 interacting proteins, and the interacting protein hnRNPM was selected for IP and immunofluorescence verification, and it was verified that the Smad3 gene had a certain promoting effect on the expression of hnRNPM gene, and then had an inhibitory effect on IL-6. It lays a foundation for further study of the function of Smad3 gene and its involved regulatory network.
Language	English
Publishing date	2024-03-26
Publishing country	United States
Document type	Journal Article
ZDB-ID	356334-0
ISSN	1089-8646 ; 0888-7543
ISSN (online)	1089-8646
ISSN	0888-7543
DOI	10.1016/j.ygeno.2024.110839
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Zs.A 2367: Show issues

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Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

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Article: Modulation of GABA

Wu, Nan / Sun, Tao / Wu, Xin / Chen, Hongguang / Zhang, Zhen

Frontiers in behavioral neuroscience

2023 Volume 16, Page(s) 1042227

Abstract: Background: Stimulation of gamma-aminobutyric acid (GABA) activity through GABA receptor agonists is the basic mechanism of many anticonvulsant drugs. Nevertheless, many of these GABergic drugs have adverse cognitive effects. We previously found that ... ...

Abstract	Background: Stimulation of gamma-aminobutyric acid (GABA) activity through GABA receptor agonists is the basic mechanism of many anticonvulsant drugs. Nevertheless, many of these GABergic drugs have adverse cognitive effects. We previously found that GABAB receptors (GABA Methods: The lithium-pilocarpine model of temporal lobe epilepsy (TLE) was established in male Sprague-Dawley rats. A 22-gauge stainless-steel guide cannula was surgically implanted into the granular insula cortex of the epileptic rats. Baclofen (125 ng/μl, 1 μl), CGP35348 (12.5 μg/μl, 1 μl), or saline (1 μl) was slowly infused through the guide cannula. The Intellicage automated behavioral testing system was used to evaluate operant associative memory of the epileptic rats, including non-spatial operant tasks (basic nosepoke learning and skilled nosepoke learning) and spatial operant tasks (chamber position learning). The expression of the GABA Results: The Intellicage tests demonstrated that baclofen significantly impaired basic nosepoke learning, skilled nosepoke learning and chamber position learning of the epileptic rats, while CGP35348 boosted these functions. Immunofluorescence staining revealed that GB1 and GB2 were expressed in the insula of the epileptic rats, and Western blotting analysis showed that baclofen enhanced while CGP35348 inhibited the expression of these subunits. Conclusion: GABA
Language	English
Publishing date	2023-01-04
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2452960-6
ISSN	1662-5153
ISSN	1662-5153
DOI	10.3389/fnbeh.2022.1042227
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Article: Association of perceived discrimination with the risk of depression among US older adults: A prospective population-based cohort study.

Wang, Yaping / Liao, Jiaojiao / Chen, Hongguang / Tao, Liyuan / Liu, Jue

Heliyon

2023 Volume 10, Issue 1, Page(s) e23843

Abstract: Aims: Association between perceived discrimination and depression has been demonstrated in some sources of discrimination, such as age, sex, and race. However, the effects of perceived discrimination both in any domain and each domain on development of ... ...

Abstract	Aims: Association between perceived discrimination and depression has been demonstrated in some sources of discrimination, such as age, sex, and race. However, the effects of perceived discrimination both in any domain and each domain on development of depression are still unclear. We aimed to estimate the association of any and each domain of perceived discrimination with the risk of depression among US older adults. Methods: We did a population-based cohort study using eight waves (from 2006 to 2020) of data from the Health and Retirement Study (HRS), a nationally representative study of US older adults aged 51 years and above. Perceived discrimination was measured by the shortened 5-item version of Williams' discrimination scale, including five domains (less courtesy, service setting, not smart, threatened or harassed, and medical setting). Depressive symptoms were assessed with shortened 8-item version of the Center for Epidemiological Depression scale (8-item CES-D). Cox proportional hazards models were used to estimate the crude and adjusted hazards ratio (HRs) and their 95 % confidence intervals (CIs) between perceived discrimination and risk of depression, after controlling for potential confounders. Results: A total of 18502 participants were included in our final analyses. 42.8 % of them had any perceived discrimination at baseline, and the most prevalent perceived discrimination was feeling less courtesy, which was observed in 5893 people (31.6 %). During a median of 9.8 years follow-up, 44.7 % of participants developed depression. The risk of depression was 46 % (adjusted HR: 1.46, 95 % CI: 1.39-1.52) higher among people with perceived discrimination than those without. The associations between perceived discrimination in each domain and risk of depression were all prominent. Conclusions: Both any and each domain of perceived discrimination were associated with an increased risk of depression. Considering the high prevalence of perceived discrimination and the following poor health outcomes, our findings suggested the integrated measures of providing public education and diversified communication to reduce discrimination, as well as accessible emotional supports to prevent depression are urgently needed.
Language	English
Publishing date	2023-12-21
Publishing country	England
Document type	Journal Article
ZDB-ID	2835763-2
ISSN	2405-8440
ISSN	2405-8440
DOI	10.1016/j.heliyon.2023.e23843
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Article ; Online: Mechanochemical Nickel-Catalyzed Carbon-Sulfur Bond Formation between Aryl Iodides and Aromatic Sulfur Surrogates.

Hao, Xiujia / Feng, Daming / Chen, Hongguang / Huang, Peng / Guo, Fang

Chemistry (Weinheim an der Bergstrasse, Germany)

2023 Volume 29, Issue 60, Page(s) e202302119

Abstract: The formation of aromatic thioethers from C-S coupling is of great importance in synthetic chemistry. Traditional solution strategies through transition-metal catalysis generally require bulk solution, heat, and longer reaction time. Herein, a mechano- ... ...

Abstract	The formation of aromatic thioethers from C-S coupling is of great importance in synthetic chemistry. Traditional solution strategies through transition-metal catalysis generally require bulk solution, heat, and longer reaction time. Herein, a mechano-promoted sulfenylation of aryl iodides with nickel catalysis is described. The active aromatic sulfide agents are in-situ generated from aromatic thiol or disulfide and subsequently adapted in the nickel catalytic cycle, with a tolerance of broad substituted groups under optimized conditions. In addition to the gram-scale synthesis that reveals the application potential of the method, the radical trapping and competitive experiments are also conducted for the mechanistic study, thus providing a plausible mechanism rationally. Furthermore, the proposed methodology is certificated as being versatile and following the green principles with ideal calculated values of green chemistry metrics, and the comparison with other approaches for C-S bond formation is also demonstrated.
Language	English
Publishing date	2023-09-28
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1478547-X
ISSN	1521-3765 ; 0947-6539
ISSN (online)	1521-3765
ISSN	0947-6539
DOI	10.1002/chem.202302119
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Article ; Online: HPF1 regulates tendon stem/progenitor cell senescence and tendon repair via PARP1-mediated poly-ADP ribosylation of HuR.

Han, Weifeng / Gu, Dongqiang / Chen, Hongguang / Tao, Xu / Chen, Lei

Genes & genomics

2023 Volume 46, Issue 1, Page(s) 27–36

Abstract: Background: Tendon stem/progenitor cells (TSPCs) play a vital role in tendon repair, regeneration and homeostasis. However, the specific mechanism of TSPCs aging is still unclear.: Objective: This study aims to explore the role and molecular ... ...

Abstract	Background: Tendon stem/progenitor cells (TSPCs) play a vital role in tendon repair, regeneration and homeostasis. However, the specific mechanism of TSPCs aging is still unclear. Objective: This study aims to explore the role and molecular mechanism of HPF1 in the aging of TSPCs. Methods: Young and aged TSPCs (Y-TSPCs and A-TSPCs) were acquired from 3 to 4 and 24-26-month-old Sprague-Dawley male rats, TSPCs (Y-TSPCs and A-TSPCs) were subjected to senescence-associated β-galactosidase (SA-β-Gal))staining and telomerase activity detection, p16, p21, Scx, Tnmd, Col1, Col3HPF1 and PAPR1 expression levels were detected by Western blot or Reverse Transcription-quantitative Polymerase Chain Reaction (RT-qPCR), Reciprocal co-immunoprecipitation (co-IP) was used to explore the interaction between HPF1 and PARP1. Ribonucleoprotein immunoprecipitation (RNP-IP) was used to analyze the binding of HuR to the senescence marker gene mRNAs, IP was used to perform HPF1 to the PARylation of HuR, and the half-life of p16 and p21 were detected. Finally, we established an in vivo model, and the tendon tissue was used to perform hematoxylin and eosin (HE) and masson's trichrome staining, as well as the immunohistochemical analysis of Col I and TNMD. Results: Compared with Y-TSPCs, A-TSPCs had significantly enhanced cell senescence and significantly reduced tendon differentiation ability, and significantly increased the expression of HPF1 and PARP1. In addition, HPF1 and PARP1 interacted and coordinated the senescence and differentiation of TSPCs, HPF1 could also regulate the expression of p21 and p21, the interaction of p16 or p21 with HuR, and the poly-ADP ribosylation of PARP1 to HuR. HPF1 overexpression and siHuR co-transfection significantly reduced the half-life of p16 and p21, and HPF1 and PARP1 regulated the mRNA levels of p16 and p21 through HuR. Finally, in vivo experiments have shown that HPF1 or PARP1 overexpression could both inhibit the ability of tendon differentiation and promote cell senescence. Conclusions: HPF1 promoted the senescence of TSPCs and inhibits the tendon differentiation of TSPCs through PARP1-mediated poly-ADP ribosylation of HuR.
MeSH term(s)	Rats ; Animals ; Male ; Poly ADP Ribosylation ; Rats, Sprague-Dawley ; Cellular Senescence ; Tendons/metabolism ; Stem Cells/metabolism
Language	English
Publishing date	2023-09-15
Publishing country	Korea (South)
Document type	Journal Article
ZDB-ID	2504587-8
ISSN	2092-9293 ; 1976-9571
ISSN (online)	2092-9293
ISSN	1976-9571
DOI	10.1007/s13258-023-01447-w
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Article ; Online: Insight into the impact of the COVID-19 epidemic on tuberculosis burden in China.

Chen, Hongguang / Zhang, Konglai

The European respiratory journal

2020 Volume 56, Issue 3

MeSH term(s)	Betacoronavirus ; COVID-19 ; China ; Coronavirus Infections/epidemiology ; Humans ; Pandemics ; Pneumonia, Viral/epidemiology ; SARS-CoV-2 ; Tuberculosis
Keywords	covid19
Language	English
Publishing date	2020-09-24
Publishing country	England
Document type	Letter ; Comment
ZDB-ID	639359-7
ISSN	1399-3003 ; 0903-1936
ISSN (online)	1399-3003
ISSN	0903-1936
DOI	10.1183/13993003.02710-2020
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Zs.A 2322: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (2.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MO 292: Show issues

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Article ; Online: Insight into the psychological problems on the epidemic of COVID-19 in China by online searching behaviors.

Chen, Hongguang / Zhang, Konglai

Journal of affective disorders

2020 Volume 276, Page(s) 1093–1094

MeSH term(s)	Animals ; Appetitive Behavior ; Betacoronavirus ; COVID-19 ; China ; Coronavirus Infections ; Humans ; Mental Health Services ; Pandemics ; Pneumonia, Viral ; SARS-CoV-2
Keywords	covid19
Language	English
Publishing date	2020-08-01
Publishing country	Netherlands
Document type	Letter ; Comment
ZDB-ID	135449-8
ISSN	1573-2517 ; 0165-0327
ISSN (online)	1573-2517
ISSN	0165-0327
DOI	10.1016/j.jad.2020.07.128
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