Article ; Online: A sandwich ELISA for the detection of mollusks and mollusk products.
2023 Volume 427, Page(s) 136732
Abstract: Mollusks are a major allergenic food under the food allergen regulations of many countries and must be declared on food products to reduce the risk of allergic reactions. However, a reliable immunoassay for detecting edible mollusks (cephalopods, ... ...
Abstract | Mollusks are a major allergenic food under the food allergen regulations of many countries and must be declared on food products to reduce the risk of allergic reactions. However, a reliable immunoassay for detecting edible mollusks (cephalopods, gastropods, and bivalves) has not been reported. In this study, the developed sandwich enzyme-linked immunosorbent assay (sELISA) detected 32 edible mollusk species in raw and heated without cross-reaction with non-mollusk species. The detection limits of the assay were 0.1 ppm for heated mollusks and 0.1-0.5 ppm for raw mollusks, depending on the mollusk species tested. The inter-assay and intra-assay coefficients of variation (CVs) were ≤14.83 and ≤8.11, respectively. The assay detected steamed, boiled, baked, fried, and autoclaved mollusk samples and all commercial mollusk products tested. In this study, we developed a mollusk-specific sELISA to protect people allergic to mollusks. |
---|---|
MeSH term(s) | Humans ; Animals ; Enzyme-Linked Immunosorbent Assay ; Food Hypersensitivity ; Bivalvia ; Gastropoda ; Allergens/analysis |
Chemical Substances | Allergens |
Language | English |
Publishing date | 2023-06-26 |
Publishing country | England |
Document type | Journal Article |
ZDB-ID | 243123-3 |
ISSN | 1873-7072 ; 0308-8146 |
ISSN (online) | 1873-7072 |
ISSN | 0308-8146 |
DOI | 10.1016/j.foodchem.2023.136732 |
Database | MEDical Literature Analysis and Retrieval System OnLINE |
More links
Kategorien
In stock of ZB MED Bonn / Germany
Z 3634: Show issues |
Order via subito
This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.
Inter-library loan at ZB MED
Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.